High-throughput screening of cell lysates for ganglioside synthesis

A high-throughput screen to detect the synthesis of natural and non-natural gangliosides by cell lysates has been developed and automated. Utilizing the binding specificity of cholera toxin B-subunit for the oligosaccharide moiety of the ganglioside G M1, the synthesis of sugar–sphingolipid glycosid...

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Veröffentlicht in:	Analytical biochemistry 2008-11, Vol.382 (1), p.48-54
Hauptverfasser:	Hancock, Susan M., Tarling, Chris A., Withers, Stephen G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies - metabolism Biotin - metabolism Biotinylation Catalysis Cattle Cell Extracts - analysis Cell Extracts - chemistry Chemical modification Cholera Toxin - metabolism Cholera toxin B-subunit Endoglycoceramidase Fluorides - chemistry Fluorides - metabolism Ganglioside Gangliosides - biosynthesis Glycosidase Glycoside Hydrolases - metabolism Glycosphingolipid Glycosynthase High-throughput screening Horseradish Peroxidase - metabolism Mice Sphingosine - metabolism Streptavidin - metabolism
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