Scopoletin‐standardized Morinda elliptica leaf extract suppressed inflammation and cartilage degradation to alleviate osteoarthritis: A preclinical study
The effect of scopoletin‐standardized Morinda elliptica leaf extract against osteoarthritis was investigated in ex vivo explant culture and preclinical rodent model. Thirty male rats were grouped (n = 6) into untreated osteoarthritis (OA), OA + Diclofenac (5 mg/kg), and OA + extract (200 and 400 mg/...
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| Veröffentlicht in: | Phytotherapy research 2017-12, Vol.31 (12), p.1954-1961 |
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| creator | Wan Osman, Wan Nurfarahin Lau, Seng Fong Mohamed, Suhaila |
| description | The effect of scopoletin‐standardized Morinda elliptica leaf extract against osteoarthritis was investigated in ex vivo explant culture and preclinical rodent model. Thirty male rats were grouped (n = 6) into untreated osteoarthritis (OA), OA + Diclofenac (5 mg/kg), and OA + extract (200 and 400 mg/kg) and compared with healthy control. Monosodium iodoacetate were injected into the right intra‐articular knee joints to induce OA. The rats were evaluated for OA severity via physical (micro‐CT and histological observations), biochemical, ELISA, and mRNA expression analysis (for inflammation and cartilage degradation biomarkers), after 28 days of treatment. The extract suppressed glycosaminoglycan release from the cartilage explant in the presence of Interleukin‐1β. The 200 mg/kg dose appeared better than 400 mg/kg dose, at reducing cartilage and subchondral bone erosions in OA‐induced rats, by significantly down‐regulating the collagenases and aggrecanase. The extract dose‐dependently reduced serum inflammation biomarkers and increased bone formation biomarkers to near normal levels in the OA‐induced rats. M. elliptica leaf scopoletin‐standardised extract alleviated OA progression and articular cartilage structure, by ameliorating cartilage degradation, nitric oxide levels, inflammation, bone /cartilage homeostasis, collagenase/aggrecanase activities, chondrocytes survival, subchondral bone structure and integrity. |
| doi_str_mv | 10.1002/ptr.5949 |
| format | Article |
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Thirty male rats were grouped (n = 6) into untreated osteoarthritis (OA), OA + Diclofenac (5 mg/kg), and OA + extract (200 and 400 mg/kg) and compared with healthy control. Monosodium iodoacetate were injected into the right intra‐articular knee joints to induce OA. The rats were evaluated for OA severity via physical (micro‐CT and histological observations), biochemical, ELISA, and mRNA expression analysis (for inflammation and cartilage degradation biomarkers), after 28 days of treatment. The extract suppressed glycosaminoglycan release from the cartilage explant in the presence of Interleukin‐1β. The 200 mg/kg dose appeared better than 400 mg/kg dose, at reducing cartilage and subchondral bone erosions in OA‐induced rats, by significantly down‐regulating the collagenases and aggrecanase. The extract dose‐dependently reduced serum inflammation biomarkers and increased bone formation biomarkers to near normal levels in the OA‐induced rats. M. elliptica leaf scopoletin‐standardised extract alleviated OA progression and articular cartilage structure, by ameliorating cartilage degradation, nitric oxide levels, inflammation, bone /cartilage homeostasis, collagenase/aggrecanase activities, chondrocytes survival, subchondral bone structure and integrity.</description><identifier>ISSN: 0951-418X</identifier><identifier>EISSN: 1099-1573</identifier><identifier>DOI: 10.1002/ptr.5949</identifier><identifier>PMID: 29067744</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Animals ; Arthritis ; articular cartilage ; Biocompatibility ; Biomarkers ; Bone growth ; Cartilage ; Cartilage (articular) ; Cartilage diseases ; Cartilage, Articular - drug effects ; Chondrocytes ; Collagen ; Collagenase ; Computed tomography ; Degradation ; Diclofenac ; Disease Models, Animal ; Enzyme-linked immunosorbent assay ; Female ; Gene expression ; Homeostasis ; Humans ; Inflammation ; Inflammation - metabolism ; Interleukin 1 ; Joint diseases ; Knee ; Leaves ; Male ; MIA (monosodium iodoacetate) ; Middle Aged ; Morinda ; Morinda - chemistry ; Morinda Elliptica ; Nitric oxide ; Osteoarthritis ; Osteoarthritis - drug therapy ; Osteoarthritis - pathology ; Osteogenesis ; rat ; Rats ; Rodents ; Scopoletin - chemistry ; Subchondral bone</subject><ispartof>Phytotherapy research, 2017-12, Vol.31 (12), p.1954-1961</ispartof><rights>Copyright © 2017 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3499-72e0cb0175afbb038c429c0fef00acbca306d298793f4669c6cad47f51d13c3</citedby><cites>FETCH-LOGICAL-c3499-72e0cb0175afbb038c429c0fef00acbca306d298793f4669c6cad47f51d13c3</cites><orcidid>0000-0002-2954-3821</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fptr.5949$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fptr.5949$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29067744$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wan Osman, Wan Nurfarahin</creatorcontrib><creatorcontrib>Lau, Seng Fong</creatorcontrib><creatorcontrib>Mohamed, Suhaila</creatorcontrib><title>Scopoletin‐standardized Morinda elliptica leaf extract suppressed inflammation and cartilage degradation to alleviate osteoarthritis: A preclinical study</title><title>Phytotherapy research</title><addtitle>Phytother Res</addtitle><description>The effect of scopoletin‐standardized Morinda elliptica leaf extract against osteoarthritis was investigated in ex vivo explant culture and preclinical rodent model. Thirty male rats were grouped (n = 6) into untreated osteoarthritis (OA), OA + Diclofenac (5 mg/kg), and OA + extract (200 and 400 mg/kg) and compared with healthy control. Monosodium iodoacetate were injected into the right intra‐articular knee joints to induce OA. The rats were evaluated for OA severity via physical (micro‐CT and histological observations), biochemical, ELISA, and mRNA expression analysis (for inflammation and cartilage degradation biomarkers), after 28 days of treatment. The extract suppressed glycosaminoglycan release from the cartilage explant in the presence of Interleukin‐1β. The 200 mg/kg dose appeared better than 400 mg/kg dose, at reducing cartilage and subchondral bone erosions in OA‐induced rats, by significantly down‐regulating the collagenases and aggrecanase. The extract dose‐dependently reduced serum inflammation biomarkers and increased bone formation biomarkers to near normal levels in the OA‐induced rats. M. elliptica leaf scopoletin‐standardised extract alleviated OA progression and articular cartilage structure, by ameliorating cartilage degradation, nitric oxide levels, inflammation, bone /cartilage homeostasis, collagenase/aggrecanase activities, chondrocytes survival, subchondral bone structure and integrity.</description><subject>Animals</subject><subject>Arthritis</subject><subject>articular cartilage</subject><subject>Biocompatibility</subject><subject>Biomarkers</subject><subject>Bone growth</subject><subject>Cartilage</subject><subject>Cartilage (articular)</subject><subject>Cartilage diseases</subject><subject>Cartilage, Articular - drug effects</subject><subject>Chondrocytes</subject><subject>Collagen</subject><subject>Collagenase</subject><subject>Computed tomography</subject><subject>Degradation</subject><subject>Diclofenac</subject><subject>Disease Models, Animal</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Female</subject><subject>Gene expression</subject><subject>Homeostasis</subject><subject>Humans</subject><subject>Inflammation</subject><subject>Inflammation - metabolism</subject><subject>Interleukin 1</subject><subject>Joint diseases</subject><subject>Knee</subject><subject>Leaves</subject><subject>Male</subject><subject>MIA (monosodium iodoacetate)</subject><subject>Middle Aged</subject><subject>Morinda</subject><subject>Morinda - chemistry</subject><subject>Morinda Elliptica</subject><subject>Nitric oxide</subject><subject>Osteoarthritis</subject><subject>Osteoarthritis - drug therapy</subject><subject>Osteoarthritis - pathology</subject><subject>Osteogenesis</subject><subject>rat</subject><subject>Rats</subject><subject>Rodents</subject><subject>Scopoletin - chemistry</subject><subject>Subchondral bone</subject><issn>0951-418X</issn><issn>1099-1573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc1u1TAQRi0EopeCxBMgS2zYpIwTJ7lmV1X8SUUg2gW7aGKPiysnDrYDXFY8AnvejifB5RaQkFiNRnN0ZkYfY_cFHAmA-vGS41GrpLrBNgKUqkTbNzfZBlQrKim27w7YnZQuAUDVIG-zg1pB1_dSbtj3Mx2W4Cm7-cfXbynjbDAa94UMfxWiKx0n792SnUbuCS2nzzmizjytyxIppUK62XqcJswuzLwYuMaYnccL4oYuIpr9JAeO3tNHh5l4SJlCwd5Hl116wo95sWnv5rLI85RXs7vLbln0ie5d10N29uzp-cmL6vT185cnx6eVbmR5tq8J9Aiib9GOIzRbLWulwZIFQD1qbKAztdr2qrGy65TuNBrZ21YY0ejmkD3aW5cYPqyU8jC5pMvTOFNY0yBU23aibzpZ0If_oJdhjXO5rVB9XUtoAf4KdQwpRbLDEt2EcTcIGK7iGkpcw1VcBX1wLVzHicwf8Hc-Baj2wCfnafdf0fDm_O0v4U9-faQy</recordid><startdate>201712</startdate><enddate>201712</enddate><creator>Wan Osman, Wan Nurfarahin</creator><creator>Lau, Seng Fong</creator><creator>Mohamed, Suhaila</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-2954-3821</orcidid></search><sort><creationdate>201712</creationdate><title>Scopoletin‐standardized Morinda elliptica leaf extract suppressed inflammation and cartilage degradation to alleviate osteoarthritis: A preclinical study</title><author>Wan Osman, Wan Nurfarahin ; Lau, Seng Fong ; Mohamed, Suhaila</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3499-72e0cb0175afbb038c429c0fef00acbca306d298793f4669c6cad47f51d13c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Arthritis</topic><topic>articular cartilage</topic><topic>Biocompatibility</topic><topic>Biomarkers</topic><topic>Bone growth</topic><topic>Cartilage</topic><topic>Cartilage (articular)</topic><topic>Cartilage diseases</topic><topic>Cartilage, Articular - drug effects</topic><topic>Chondrocytes</topic><topic>Collagen</topic><topic>Collagenase</topic><topic>Computed tomography</topic><topic>Degradation</topic><topic>Diclofenac</topic><topic>Disease Models, Animal</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Female</topic><topic>Gene expression</topic><topic>Homeostasis</topic><topic>Humans</topic><topic>Inflammation</topic><topic>Inflammation - metabolism</topic><topic>Interleukin 1</topic><topic>Joint diseases</topic><topic>Knee</topic><topic>Leaves</topic><topic>Male</topic><topic>MIA (monosodium iodoacetate)</topic><topic>Middle Aged</topic><topic>Morinda</topic><topic>Morinda - chemistry</topic><topic>Morinda Elliptica</topic><topic>Nitric oxide</topic><topic>Osteoarthritis</topic><topic>Osteoarthritis - drug therapy</topic><topic>Osteoarthritis - pathology</topic><topic>Osteogenesis</topic><topic>rat</topic><topic>Rats</topic><topic>Rodents</topic><topic>Scopoletin - chemistry</topic><topic>Subchondral bone</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wan Osman, Wan Nurfarahin</creatorcontrib><creatorcontrib>Lau, Seng Fong</creatorcontrib><creatorcontrib>Mohamed, Suhaila</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Phytotherapy research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wan Osman, Wan Nurfarahin</au><au>Lau, Seng Fong</au><au>Mohamed, Suhaila</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Scopoletin‐standardized Morinda elliptica leaf extract suppressed inflammation and cartilage degradation to alleviate osteoarthritis: A preclinical study</atitle><jtitle>Phytotherapy research</jtitle><addtitle>Phytother Res</addtitle><date>2017-12</date><risdate>2017</risdate><volume>31</volume><issue>12</issue><spage>1954</spage><epage>1961</epage><pages>1954-1961</pages><issn>0951-418X</issn><eissn>1099-1573</eissn><abstract>The effect of scopoletin‐standardized Morinda elliptica leaf extract against osteoarthritis was investigated in ex vivo explant culture and preclinical rodent model. Thirty male rats were grouped (n = 6) into untreated osteoarthritis (OA), OA + Diclofenac (5 mg/kg), and OA + extract (200 and 400 mg/kg) and compared with healthy control. Monosodium iodoacetate were injected into the right intra‐articular knee joints to induce OA. The rats were evaluated for OA severity via physical (micro‐CT and histological observations), biochemical, ELISA, and mRNA expression analysis (for inflammation and cartilage degradation biomarkers), after 28 days of treatment. The extract suppressed glycosaminoglycan release from the cartilage explant in the presence of Interleukin‐1β. The 200 mg/kg dose appeared better than 400 mg/kg dose, at reducing cartilage and subchondral bone erosions in OA‐induced rats, by significantly down‐regulating the collagenases and aggrecanase. The extract dose‐dependently reduced serum inflammation biomarkers and increased bone formation biomarkers to near normal levels in the OA‐induced rats. M. elliptica leaf scopoletin‐standardised extract alleviated OA progression and articular cartilage structure, by ameliorating cartilage degradation, nitric oxide levels, inflammation, bone /cartilage homeostasis, collagenase/aggrecanase activities, chondrocytes survival, subchondral bone structure and integrity.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>29067744</pmid><doi>10.1002/ptr.5949</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-2954-3821</orcidid></addata></record> |
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| subjects | Animals Arthritis articular cartilage Biocompatibility Biomarkers Bone growth Cartilage Cartilage (articular) Cartilage diseases Cartilage, Articular - drug effects Chondrocytes Collagen Collagenase Computed tomography Degradation Diclofenac Disease Models, Animal Enzyme-linked immunosorbent assay Female Gene expression Homeostasis Humans Inflammation Inflammation - metabolism Interleukin 1 Joint diseases Knee Leaves Male MIA (monosodium iodoacetate) Middle Aged Morinda Morinda - chemistry Morinda Elliptica Nitric oxide Osteoarthritis Osteoarthritis - drug therapy Osteoarthritis - pathology Osteogenesis rat Rats Rodents Scopoletin - chemistry Subchondral bone |
| title | Scopoletin‐standardized Morinda elliptica leaf extract suppressed inflammation and cartilage degradation to alleviate osteoarthritis: A preclinical study |
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