Downregulation of microRNA-448 improves isoflurane-induced learning and memory impairment in rats
The present study aimed to investigate the potential role of microRNA-448 (miR-448) in isoflurane-induced learning and memory impairment in rats. Sprague-Dawley rats were used for the construction of isoflurane-treated models. The Morris water maze test was used to evaluate the effects of isoflurane...
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| Veröffentlicht in: | Molecular medicine reports 2017-08, Vol.16 (2), p.1578-1583 |
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| creator | Wu, Qun Dai, Qinxue Jiang, Liuming Wang, Yichuan Yang, Tao Miao, Jianxia Wang, Junlu Han, Yu |
| description | The present study aimed to investigate the potential role of microRNA-448 (miR-448) in isoflurane-induced learning and memory impairment in rats. Sprague-Dawley rats were used for the construction of isoflurane-treated models. The Morris water maze test was used to evaluate the effects of isoflurane on rats regarding the following parameters: Swimming speed, escape latency and time in original quadrant. Influences of isoflurane on neuron apoptosis and miR-448 expression in rat hippocampus tissue were analyzed by flow cytometry and reverse transcription-quantitative polymerase chain reaction, respectively. Furthermore, the effects of miR-448 on the expression of cell apoptosis-associated proteins were investigated by flow cytometry. The results demonstrated that isoflurane treatment induced higher escape latency and lower time spent in original quadrant compared with the control rats. In addition, isoflurane treatment induced neuron apoptosis and miR-448 was highly expressed in the hippocampal tissue of isoflurane-treated rats. Furthermore, Bcl-x was significantly downregulated while caspase-3 expression was upregulated by an miR-448 inhibitor. Combined the results of the current study indicate that miR-448 knockdown may have pivotal roles in improving isoflurane-induced learning and memory impairment via suppressing neuron apoptosis. |
| doi_str_mv | 10.3892/mmr.2017.6724 |
| format | Article |
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Sprague-Dawley rats were used for the construction of isoflurane-treated models. The Morris water maze test was used to evaluate the effects of isoflurane on rats regarding the following parameters: Swimming speed, escape latency and time in original quadrant. Influences of isoflurane on neuron apoptosis and miR-448 expression in rat hippocampus tissue were analyzed by flow cytometry and reverse transcription-quantitative polymerase chain reaction, respectively. Furthermore, the effects of miR-448 on the expression of cell apoptosis-associated proteins were investigated by flow cytometry. The results demonstrated that isoflurane treatment induced higher escape latency and lower time spent in original quadrant compared with the control rats. In addition, isoflurane treatment induced neuron apoptosis and miR-448 was highly expressed in the hippocampal tissue of isoflurane-treated rats. Furthermore, Bcl-x was significantly downregulated while caspase-3 expression was upregulated by an miR-448 inhibitor. Combined the results of the current study indicate that miR-448 knockdown may have pivotal roles in improving isoflurane-induced learning and memory impairment via suppressing neuron apoptosis.</description><identifier>ISSN: 1791-2997</identifier><identifier>EISSN: 1791-3004</identifier><identifier>DOI: 10.3892/mmr.2017.6724</identifier><identifier>PMID: 29067448</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>Alzheimer's disease ; Anesthesia ; Animal models ; Animals ; Antagomirs - metabolism ; Apoptosis ; Apoptosis - drug effects ; bcl-2-Associated X Protein - metabolism ; Bcl-x protein ; Care and treatment ; Caspase ; Caspase 3 - metabolism ; Caspase-3 ; cell apoptosis ; Cells, Cultured ; Cognitive disorders ; Complications and side effects ; Down-Regulation ; Flow cytometry ; Gene expression ; Genetic aspects ; Genetic regulation ; Health aspects ; Hippocampus ; Hippocampus - cytology ; Hippocampus - drug effects ; Hippocampus - metabolism ; Isoflurane ; Isoflurane - pharmacology ; Laboratory animals ; Latency ; Learning ; learning and memory impairment ; Male ; Maze Learning - drug effects ; Memory ; Memory - drug effects ; MicroRNA ; microRNA-448 ; MicroRNAs - antagonists & inhibitors ; MicroRNAs - genetics ; MicroRNAs - metabolism ; miRNA ; Neurons ; Polymerase chain reaction ; Properties ; Proteins ; Rats ; Rats, Sprague-Dawley ; Reverse transcription ; Rodents ; Studies ; Swimming</subject><ispartof>Molecular medicine reports, 2017-08, Vol.16 (2), p.1578-1583</ispartof><rights>Copyright © 2017, Spandidos Publications</rights><rights>COPYRIGHT 2017 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c525t-bc063f5fafef947c57f45d0974166155f5342d480cd9bbb7ed8400765eeeec383</citedby><cites>FETCH-LOGICAL-c525t-bc063f5fafef947c57f45d0974166155f5342d480cd9bbb7ed8400765eeeec383</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,5571,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29067448$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Qun</creatorcontrib><creatorcontrib>Dai, Qinxue</creatorcontrib><creatorcontrib>Jiang, Liuming</creatorcontrib><creatorcontrib>Wang, Yichuan</creatorcontrib><creatorcontrib>Yang, Tao</creatorcontrib><creatorcontrib>Miao, Jianxia</creatorcontrib><creatorcontrib>Wang, Junlu</creatorcontrib><creatorcontrib>Han, Yu</creatorcontrib><title>Downregulation of microRNA-448 improves isoflurane-induced learning and memory impairment in rats</title><title>Molecular medicine reports</title><addtitle>Mol Med Rep</addtitle><description>The present study aimed to investigate the potential role of microRNA-448 (miR-448) in isoflurane-induced learning and memory impairment in rats. Sprague-Dawley rats were used for the construction of isoflurane-treated models. The Morris water maze test was used to evaluate the effects of isoflurane on rats regarding the following parameters: Swimming speed, escape latency and time in original quadrant. Influences of isoflurane on neuron apoptosis and miR-448 expression in rat hippocampus tissue were analyzed by flow cytometry and reverse transcription-quantitative polymerase chain reaction, respectively. Furthermore, the effects of miR-448 on the expression of cell apoptosis-associated proteins were investigated by flow cytometry. The results demonstrated that isoflurane treatment induced higher escape latency and lower time spent in original quadrant compared with the control rats. In addition, isoflurane treatment induced neuron apoptosis and miR-448 was highly expressed in the hippocampal tissue of isoflurane-treated rats. Furthermore, Bcl-x was significantly downregulated while caspase-3 expression was upregulated by an miR-448 inhibitor. Combined the results of the current study indicate that miR-448 knockdown may have pivotal roles in improving isoflurane-induced learning and memory impairment via suppressing neuron apoptosis.</description><subject>Alzheimer's disease</subject><subject>Anesthesia</subject><subject>Animal models</subject><subject>Animals</subject><subject>Antagomirs - metabolism</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>bcl-2-Associated X Protein - metabolism</subject><subject>Bcl-x protein</subject><subject>Care and treatment</subject><subject>Caspase</subject><subject>Caspase 3 - metabolism</subject><subject>Caspase-3</subject><subject>cell apoptosis</subject><subject>Cells, Cultured</subject><subject>Cognitive disorders</subject><subject>Complications and side effects</subject><subject>Down-Regulation</subject><subject>Flow cytometry</subject><subject>Gene expression</subject><subject>Genetic aspects</subject><subject>Genetic regulation</subject><subject>Health aspects</subject><subject>Hippocampus</subject><subject>Hippocampus - cytology</subject><subject>Hippocampus - drug effects</subject><subject>Hippocampus - metabolism</subject><subject>Isoflurane</subject><subject>Isoflurane - pharmacology</subject><subject>Laboratory animals</subject><subject>Latency</subject><subject>Learning</subject><subject>learning and memory impairment</subject><subject>Male</subject><subject>Maze Learning - drug effects</subject><subject>Memory</subject><subject>Memory - drug effects</subject><subject>MicroRNA</subject><subject>microRNA-448</subject><subject>MicroRNAs - antagonists & inhibitors</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>miRNA</subject><subject>Neurons</subject><subject>Polymerase chain reaction</subject><subject>Properties</subject><subject>Proteins</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reverse transcription</subject><subject>Rodents</subject><subject>Studies</subject><subject>Swimming</subject><issn>1791-2997</issn><issn>1791-3004</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNptkctrHSEYxYfS0jzaZbdF6KLZeKMzPsblJX0FQgOlXYsz6sUw6q3OpOS_7zfc25SW6EKR33c8nNM0byjZdL1qL2Msm5ZQuRGyZc-aUyoVxR0h7Pnx3iolT5qzWu8IEbzl6mVz0ioiJGP9aWM-5F-puN0ymTnkhLJHMYwlf_u6xQCgEPcl37uKQs1-WopJDodkl9FZNDlTUkg7ZJJF0cVcHlbehBJdmlFIqJi5vmpeeDNV9_p4njc_Pn38fvUF39x-vr7a3uARTM14GInoPPfGO6-YHLn0jFuiJKNCUM4971hrWU9Gq4ZhkM72jBApuIM1dn133lwcdMHwz8XVWcdQRzdNYDkvVVPFuaBCMQLou__Qu7yUBO6AEhARfMX-UjszOR2Sz3Mx4yqqt5ywjlHWKqA2T1CwrYMgc3I-wPs_A_gwACnXWpzX-xKiKQ-aEr1WqqFSvVaq10qBf3s0uwzR2Uf6T4cAvD8AdQ9FBJvrIwNKmApMWky57LvfSPGoCg</recordid><startdate>20170801</startdate><enddate>20170801</enddate><creator>Wu, Qun</creator><creator>Dai, Qinxue</creator><creator>Jiang, Liuming</creator><creator>Wang, Yichuan</creator><creator>Yang, Tao</creator><creator>Miao, Jianxia</creator><creator>Wang, Junlu</creator><creator>Han, Yu</creator><general>D.A. Spandidos</general><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20170801</creationdate><title>Downregulation of microRNA-448 improves isoflurane-induced learning and memory impairment in rats</title><author>Wu, Qun ; Dai, Qinxue ; Jiang, Liuming ; Wang, Yichuan ; Yang, Tao ; Miao, Jianxia ; Wang, Junlu ; Han, Yu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c525t-bc063f5fafef947c57f45d0974166155f5342d480cd9bbb7ed8400765eeeec383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Alzheimer's disease</topic><topic>Anesthesia</topic><topic>Animal models</topic><topic>Animals</topic><topic>Antagomirs - metabolism</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>bcl-2-Associated X Protein - metabolism</topic><topic>Bcl-x protein</topic><topic>Care and treatment</topic><topic>Caspase</topic><topic>Caspase 3 - metabolism</topic><topic>Caspase-3</topic><topic>cell apoptosis</topic><topic>Cells, Cultured</topic><topic>Cognitive disorders</topic><topic>Complications and side effects</topic><topic>Down-Regulation</topic><topic>Flow cytometry</topic><topic>Gene expression</topic><topic>Genetic aspects</topic><topic>Genetic regulation</topic><topic>Health aspects</topic><topic>Hippocampus</topic><topic>Hippocampus - cytology</topic><topic>Hippocampus - drug effects</topic><topic>Hippocampus - metabolism</topic><topic>Isoflurane</topic><topic>Isoflurane - pharmacology</topic><topic>Laboratory animals</topic><topic>Latency</topic><topic>Learning</topic><topic>learning and memory impairment</topic><topic>Male</topic><topic>Maze Learning - drug effects</topic><topic>Memory</topic><topic>Memory - drug effects</topic><topic>MicroRNA</topic><topic>microRNA-448</topic><topic>MicroRNAs - antagonists & inhibitors</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>miRNA</topic><topic>Neurons</topic><topic>Polymerase chain reaction</topic><topic>Properties</topic><topic>Proteins</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reverse transcription</topic><topic>Rodents</topic><topic>Studies</topic><topic>Swimming</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Qun</creatorcontrib><creatorcontrib>Dai, Qinxue</creatorcontrib><creatorcontrib>Jiang, Liuming</creatorcontrib><creatorcontrib>Wang, Yichuan</creatorcontrib><creatorcontrib>Yang, Tao</creatorcontrib><creatorcontrib>Miao, Jianxia</creatorcontrib><creatorcontrib>Wang, Junlu</creatorcontrib><creatorcontrib>Han, Yu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular medicine reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Qun</au><au>Dai, Qinxue</au><au>Jiang, Liuming</au><au>Wang, Yichuan</au><au>Yang, Tao</au><au>Miao, Jianxia</au><au>Wang, Junlu</au><au>Han, Yu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Downregulation of microRNA-448 improves isoflurane-induced learning and memory impairment in rats</atitle><jtitle>Molecular medicine reports</jtitle><addtitle>Mol Med Rep</addtitle><date>2017-08-01</date><risdate>2017</risdate><volume>16</volume><issue>2</issue><spage>1578</spage><epage>1583</epage><pages>1578-1583</pages><issn>1791-2997</issn><eissn>1791-3004</eissn><abstract>The present study aimed to investigate the potential role of microRNA-448 (miR-448) in isoflurane-induced learning and memory impairment in rats. Sprague-Dawley rats were used for the construction of isoflurane-treated models. The Morris water maze test was used to evaluate the effects of isoflurane on rats regarding the following parameters: Swimming speed, escape latency and time in original quadrant. Influences of isoflurane on neuron apoptosis and miR-448 expression in rat hippocampus tissue were analyzed by flow cytometry and reverse transcription-quantitative polymerase chain reaction, respectively. Furthermore, the effects of miR-448 on the expression of cell apoptosis-associated proteins were investigated by flow cytometry. The results demonstrated that isoflurane treatment induced higher escape latency and lower time spent in original quadrant compared with the control rats. In addition, isoflurane treatment induced neuron apoptosis and miR-448 was highly expressed in the hippocampal tissue of isoflurane-treated rats. Furthermore, Bcl-x was significantly downregulated while caspase-3 expression was upregulated by an miR-448 inhibitor. Combined the results of the current study indicate that miR-448 knockdown may have pivotal roles in improving isoflurane-induced learning and memory impairment via suppressing neuron apoptosis.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>29067448</pmid><doi>10.3892/mmr.2017.6724</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Molecular medicine reports, 2017-08, Vol.16 (2), p.1578-1583 |
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| source | Spandidos Publications Journals; MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Alzheimer's disease Anesthesia Animal models Animals Antagomirs - metabolism Apoptosis Apoptosis - drug effects bcl-2-Associated X Protein - metabolism Bcl-x protein Care and treatment Caspase Caspase 3 - metabolism Caspase-3 cell apoptosis Cells, Cultured Cognitive disorders Complications and side effects Down-Regulation Flow cytometry Gene expression Genetic aspects Genetic regulation Health aspects Hippocampus Hippocampus - cytology Hippocampus - drug effects Hippocampus - metabolism Isoflurane Isoflurane - pharmacology Laboratory animals Latency Learning learning and memory impairment Male Maze Learning - drug effects Memory Memory - drug effects MicroRNA microRNA-448 MicroRNAs - antagonists & inhibitors MicroRNAs - genetics MicroRNAs - metabolism miRNA Neurons Polymerase chain reaction Properties Proteins Rats Rats, Sprague-Dawley Reverse transcription Rodents Studies Swimming |
| title | Downregulation of microRNA-448 improves isoflurane-induced learning and memory impairment in rats |
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