Differentiation of pathogenic leptospires spp by PCR of ligB gene and sequencing
Leptospirosis is a zoonosis having worldwide distribution. The objective of this work was to develop a molecular technique to differentiate pathogenic Leptospira spp. A region of adhesin ligB, present only in the pathogenic species was amplified by PCR and sequenced. ligBRpet and ligBFpet primers we...
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| Veröffentlicht in: | Revista argentina de microbiología 2018-04, Vol.50 (2), p.126-130 |
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| container_title | Revista argentina de microbiología |
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| creator | Martínez, Mara Leila Grune Loffler, Sylvia Romero, Graciela Noemi Brihuega, Bibiana Felicitas |
| description | Leptospirosis is a zoonosis having worldwide distribution. The objective of this work was to develop a molecular technique to differentiate pathogenic Leptospira spp. A region of adhesin ligB, present only in the pathogenic species was amplified by PCR and sequenced. ligBRpet and ligBFpet primers were used, which amplified the target DNA from pathogenic L. interrogans reference strains serovars Pomona strain Pomona, Canicola strain Hond Utrecht IV, Copenhageni strain M 20, Wolffi strain 3705, Pyrogenes strain Salinem, Hardjo strain Hardjoprajitmo, L. borgpetersenii serovar Castellonis strain Castellon 3 and 4 pathogenic strains isolated from bovines, pigs, rats and opossums. L. biflexa serovars Patoc strain Patoc I and Andamana strain Andamana were not amplified. Sequencing of the amplified products exhibited sufficient variation among serovars, which differentiates them. |
| doi_str_mv | 10.1016/j.ram.2016.11.008 |
| format | Article |
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The objective of this work was to develop a molecular technique to differentiate pathogenic Leptospira spp. A region of adhesin ligB, present only in the pathogenic species was amplified by PCR and sequenced. ligBRpet and ligBFpet primers were used, which amplified the target DNA from pathogenic L. interrogans reference strains serovars Pomona strain Pomona, Canicola strain Hond Utrecht IV, Copenhageni strain M 20, Wolffi strain 3705, Pyrogenes strain Salinem, Hardjo strain Hardjoprajitmo, L. borgpetersenii serovar Castellonis strain Castellon 3 and 4 pathogenic strains isolated from bovines, pigs, rats and opossums. L. biflexa serovars Patoc strain Patoc I and Andamana strain Andamana were not amplified. 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The objective of this work was to develop a molecular technique to differentiate pathogenic Leptospira spp. A region of adhesin ligB, present only in the pathogenic species was amplified by PCR and sequenced. ligBRpet and ligBFpet primers were used, which amplified the target DNA from pathogenic L. interrogans reference strains serovars Pomona strain Pomona, Canicola strain Hond Utrecht IV, Copenhageni strain M 20, Wolffi strain 3705, Pyrogenes strain Salinem, Hardjo strain Hardjoprajitmo, L. borgpetersenii serovar Castellonis strain Castellon 3 and 4 pathogenic strains isolated from bovines, pigs, rats and opossums. L. biflexa serovars Patoc strain Patoc I and Andamana strain Andamana were not amplified. Sequencing of the amplified products exhibited sufficient variation among serovars, which differentiates them.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Cattle</subject><subject>DNA Primers</subject><subject>Leptospira - genetics</subject><subject>Leptospira interrogans</subject><subject>Leptospirosis - diagnosis</subject><subject>Polymerase Chain Reaction</subject><subject>Rats</subject><subject>Swine</subject><issn>0325-7541</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo10D9PwzAQBXAPIFoKH4AFeWRJuIsTOxmh_JUqUSGYIyc5B1eJY-J06LcniDLdG356TzrGrhBiBJS3u3jUfZzMMUaMAfITtgSRZJHKUlyw8xB2ACmkQpyxRVKAlCDEkm0frDE0kpusnuzg-GC419PX0JKzNe_IT0PwdqTAg_e8OvDt-v0Xdba95zMirl3DA33vydXWtRfs1Ogu0OXxrtjn0-PH-iXavD2_ru82kU8Qp0gqVYGRDWY1kVaZyWVVIyUKVY0SczANydQYAK0RC0qhaKDJiyRBVVEDYsVu_nr9OMzbYSp7G2rqOu1o2IcSiyyTgCLPZ3p9pPuqp6b0o-31eCj_vyB-AFVwXWE</recordid><startdate>201804</startdate><enddate>201804</enddate><creator>Martínez, Mara Leila</creator><creator>Grune Loffler, Sylvia</creator><creator>Romero, Graciela Noemi</creator><creator>Brihuega, Bibiana Felicitas</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201804</creationdate><title>Differentiation of pathogenic leptospires spp by PCR of ligB gene and sequencing</title><author>Martínez, Mara Leila ; Grune Loffler, Sylvia ; Romero, Graciela Noemi ; Brihuega, Bibiana Felicitas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p211t-677b0f6d15ceea75f86bc1e2717c16180fde64ff00aa119e409d0d892217bed03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>spa</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Cattle</topic><topic>DNA Primers</topic><topic>Leptospira - genetics</topic><topic>Leptospira interrogans</topic><topic>Leptospirosis - diagnosis</topic><topic>Polymerase Chain Reaction</topic><topic>Rats</topic><topic>Swine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Martínez, Mara Leila</creatorcontrib><creatorcontrib>Grune Loffler, Sylvia</creatorcontrib><creatorcontrib>Romero, Graciela Noemi</creatorcontrib><creatorcontrib>Brihuega, Bibiana Felicitas</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Revista argentina de microbiología</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Martínez, Mara Leila</au><au>Grune Loffler, Sylvia</au><au>Romero, Graciela Noemi</au><au>Brihuega, Bibiana Felicitas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differentiation of pathogenic leptospires spp by PCR of ligB gene and sequencing</atitle><jtitle>Revista argentina de microbiología</jtitle><addtitle>Rev Argent Microbiol</addtitle><date>2018-04</date><risdate>2018</risdate><volume>50</volume><issue>2</issue><spage>126</spage><epage>130</epage><pages>126-130</pages><issn>0325-7541</issn><abstract>Leptospirosis is a zoonosis having worldwide distribution. The objective of this work was to develop a molecular technique to differentiate pathogenic Leptospira spp. A region of adhesin ligB, present only in the pathogenic species was amplified by PCR and sequenced. ligBRpet and ligBFpet primers were used, which amplified the target DNA from pathogenic L. interrogans reference strains serovars Pomona strain Pomona, Canicola strain Hond Utrecht IV, Copenhageni strain M 20, Wolffi strain 3705, Pyrogenes strain Salinem, Hardjo strain Hardjoprajitmo, L. borgpetersenii serovar Castellonis strain Castellon 3 and 4 pathogenic strains isolated from bovines, pigs, rats and opossums. L. biflexa serovars Patoc strain Patoc I and Andamana strain Andamana were not amplified. Sequencing of the amplified products exhibited sufficient variation among serovars, which differentiates them.</abstract><cop>Argentina</cop><pmid>29066033</pmid><doi>10.1016/j.ram.2016.11.008</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| language | spa |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Base Sequence Cattle DNA Primers Leptospira - genetics Leptospira interrogans Leptospirosis - diagnosis Polymerase Chain Reaction Rats Swine |
| title | Differentiation of pathogenic leptospires spp by PCR of ligB gene and sequencing |
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