Nonenzymatic Sensor for Lactate Detection in Human Sweat
For noninvasive diagnostics of hypoxia, we propose the nonenzymatic sensor based on screen-printed structures with the working surface modified in course of electropolymerization of 3-aminophenylboronic acid (3-APBA) with imprinting of lactate. Impedimetric sensor allows lactate detection in the ran...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2017-11, Vol.89 (21), p.11198-11202 |
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| creator | Zaryanov, Nikolay V Nikitina, Vita N Karpova, Elena V Karyakina, Elena E Karyakin, Arkady A |
| description | For noninvasive diagnostics of hypoxia, we propose the nonenzymatic sensor based on screen-printed structures with the working surface modified in course of electropolymerization of 3-aminophenylboronic acid (3-APBA) with imprinting of lactate. Impedimetric sensor allows lactate detection in the range from 3 mM to 100 mM with the detection limit of 1.5 mM; response time is 2–3 min. Sensor sensitivity remains unchanged within 6 months of storage unpacked in dry state at a room temperature, which is unachievable for enzyme based devices. Analysis of human sweat with poly(3-APBA) based sensor is possible due to (i) much higher lactate content compared to other polyols and (ii) high sensor selectivity (K lactate glucose < 3 × 10–2). Successful detection of lactate in human sweat by means of the poly(3-APBA) based sensor has been confirmed using the highly specific reference method based on lactate oxidase enzyme (correlation coefficient r > 0.9). The attractive performance characteristics of poly(3-APBA) based enzyme-free sensors justify their future use for noninvasive clinical analysis and sports medicine. |
| doi_str_mv | 10.1021/acs.analchem.7b03662 |
| format | Article |
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Impedimetric sensor allows lactate detection in the range from 3 mM to 100 mM with the detection limit of 1.5 mM; response time is 2–3 min. Sensor sensitivity remains unchanged within 6 months of storage unpacked in dry state at a room temperature, which is unachievable for enzyme based devices. Analysis of human sweat with poly(3-APBA) based sensor is possible due to (i) much higher lactate content compared to other polyols and (ii) high sensor selectivity (K lactate glucose < 3 × 10–2). Successful detection of lactate in human sweat by means of the poly(3-APBA) based sensor has been confirmed using the highly specific reference method based on lactate oxidase enzyme (correlation coefficient r > 0.9). The attractive performance characteristics of poly(3-APBA) based enzyme-free sensors justify their future use for noninvasive clinical analysis and sports medicine.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/acs.analchem.7b03662</identifier><identifier>PMID: 29065687</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Biosensors ; Body fluids ; Boronic Acids - chemistry ; Chemistry ; Correlation coefficient ; Correlation coefficients ; Electrochemical Techniques - methods ; Enzymes ; Humans ; Hypoxia ; Lactate oxidase ; Lactic acid ; Lactic Acid - analysis ; Limit of Detection ; Polymerization ; Polymers - chemistry ; Polyols ; Response time ; Sports medicine ; Sweat ; Sweat - chemistry</subject><ispartof>Analytical chemistry (Washington), 2017-11, Vol.89 (21), p.11198-11202</ispartof><rights>Copyright © 2017 American Chemical Society</rights><rights>Copyright American Chemical Society Nov 7, 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a422t-178e51175d894b16ff70b6e8395a1bdb90c1b5d53a338200753422de601bb9193</citedby><cites>FETCH-LOGICAL-a422t-178e51175d894b16ff70b6e8395a1bdb90c1b5d53a338200753422de601bb9193</cites><orcidid>0000-0002-0457-7638</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.analchem.7b03662$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.analchem.7b03662$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2751,27055,27903,27904,56716,56766</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29065687$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zaryanov, Nikolay V</creatorcontrib><creatorcontrib>Nikitina, Vita N</creatorcontrib><creatorcontrib>Karpova, Elena V</creatorcontrib><creatorcontrib>Karyakina, Elena E</creatorcontrib><creatorcontrib>Karyakin, Arkady A</creatorcontrib><title>Nonenzymatic Sensor for Lactate Detection in Human Sweat</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>For noninvasive diagnostics of hypoxia, we propose the nonenzymatic sensor based on screen-printed structures with the working surface modified in course of electropolymerization of 3-aminophenylboronic acid (3-APBA) with imprinting of lactate. Impedimetric sensor allows lactate detection in the range from 3 mM to 100 mM with the detection limit of 1.5 mM; response time is 2–3 min. Sensor sensitivity remains unchanged within 6 months of storage unpacked in dry state at a room temperature, which is unachievable for enzyme based devices. Analysis of human sweat with poly(3-APBA) based sensor is possible due to (i) much higher lactate content compared to other polyols and (ii) high sensor selectivity (K lactate glucose < 3 × 10–2). Successful detection of lactate in human sweat by means of the poly(3-APBA) based sensor has been confirmed using the highly specific reference method based on lactate oxidase enzyme (correlation coefficient r > 0.9). The attractive performance characteristics of poly(3-APBA) based enzyme-free sensors justify their future use for noninvasive clinical analysis and sports medicine.</description><subject>Biosensors</subject><subject>Body fluids</subject><subject>Boronic Acids - chemistry</subject><subject>Chemistry</subject><subject>Correlation coefficient</subject><subject>Correlation coefficients</subject><subject>Electrochemical Techniques - methods</subject><subject>Enzymes</subject><subject>Humans</subject><subject>Hypoxia</subject><subject>Lactate oxidase</subject><subject>Lactic acid</subject><subject>Lactic Acid - analysis</subject><subject>Limit of Detection</subject><subject>Polymerization</subject><subject>Polymers - chemistry</subject><subject>Polyols</subject><subject>Response time</subject><subject>Sports medicine</subject><subject>Sweat</subject><subject>Sweat - chemistry</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9LwzAYh4Mobk6_gUjBi5fO902aND3K_AtDD9NzSdIUO9p0Ni0yP70Z2xQ8eAjv5Xl-gYeQc4QpAsVrZfxUOVWbd9tMUw1MCHpAxsgpxEJKekjGAMBimgKMyIn3SwBEQHFMRjQDwYVMx0Q-t866r3Wj-spEC-t820VleHNletXb6Nb21vRV66LKRY9Do1y0-LSqPyVHpaq9PdvdCXm7v3udPcbzl4en2c08VgmlfYyptBwx5YXMEo2iLFPQwkqWcYW60BkY1LzgTDEmKUDKWfAKKwC1zjBjE3K13V117cdgfZ83lTe2rpWz7eBzzDgPMAMZ0Ms_6LIdupBoQ0mOLFRJApVsKdO13ne2zFdd1ahunSPkm7J5KJvvy-a7skG72I0PurHFj7RPGQDYAhv99-P_Nr8BNmOFGA</recordid><startdate>20171107</startdate><enddate>20171107</enddate><creator>Zaryanov, Nikolay V</creator><creator>Nikitina, Vita N</creator><creator>Karpova, Elena V</creator><creator>Karyakina, Elena E</creator><creator>Karyakin, Arkady A</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0457-7638</orcidid></search><sort><creationdate>20171107</creationdate><title>Nonenzymatic Sensor for Lactate Detection in Human Sweat</title><author>Zaryanov, Nikolay V ; 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| subjects | Biosensors Body fluids Boronic Acids - chemistry Chemistry Correlation coefficient Correlation coefficients Electrochemical Techniques - methods Enzymes Humans Hypoxia Lactate oxidase Lactic acid Lactic Acid - analysis Limit of Detection Polymerization Polymers - chemistry Polyols Response time Sports medicine Sweat Sweat - chemistry |
| title | Nonenzymatic Sensor for Lactate Detection in Human Sweat |
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