Exhaled breath condensate methods adapted from human studies using longitudinal metabolomics for predicting early health alterations in dolphins
Monitoring health conditions is essential to detect early asymptomatic stages of a disease. To achieve this, blood, urine and breath samples are commonly used as a routine clinical diagnostic. These samples offer the opportunity to detect specific metabolites related to diseases and provide a better...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2017-11, Vol.409 (28), p.6523-6536 |
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| creator | Borras, Eva Aksenov, Alexander A. Baird, Mark Novick, Brittany Schivo, Michael Zamuruyev, Konstantin O. Pasamontes, Alberto Parry, Celeste Foutouhi, Soraya Venn-Watson, Stephanie Weimer, Bart C. Davis, Cristina E. |
| description | Monitoring health conditions is essential to detect early asymptomatic stages of a disease. To achieve this, blood, urine and breath samples are commonly used as a routine clinical diagnostic. These samples offer the opportunity to detect specific metabolites related to diseases and provide a better understanding of their development. Although blood samples are commonly used routinely to monitor health, the implementation of a relatively noninvasive technique, such as exhaled breath condensate (EBC) analysis, may further benefit the well-being of both humans and other animals. EBC analysis can be used to track possible physical or biochemical alterations caused by common diseases of the bottlenose dolphin (
Tursiops truncatus
), such as infections or inflammatory-mediated processes. We have used an untargeted metabolomic method with liquid chromatography–mass spectrometry analysis of EBC samples to determine biomarkers related to disease development. In this study, five dolphins under human care were followed up for 1 year. We collected paired blood, physical examination information, and EBC samples. We then statistically correlated this information to predict specific health alterations. Three dolphins provided promising case study information about biomarkers related to cutaneous infections, respiratory infections, dental disease, or hormonal changes (pregnancy). The use of complementary liquid chromatography platforms, with hydrophilic interaction chromatography and reverse-phased columns, allowed us to detect a wide spectrum of EBC biomarker compounds that could be related to these health alterations. Moreover, these two analytical techniques not only provided complementary metabolite information but in both cases they also provided promising diagnostic information for these health conditions.
Graphical abstract
Collection of the exhaled condensed breath from a bottlenose dolphin from U.S. Navy Marine Mammal Program (MMP) |
| doi_str_mv | 10.1007/s00216-017-0581-6 |
| format | Article |
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Tursiops truncatus
), such as infections or inflammatory-mediated processes. We have used an untargeted metabolomic method with liquid chromatography–mass spectrometry analysis of EBC samples to determine biomarkers related to disease development. In this study, five dolphins under human care were followed up for 1 year. We collected paired blood, physical examination information, and EBC samples. We then statistically correlated this information to predict specific health alterations. Three dolphins provided promising case study information about biomarkers related to cutaneous infections, respiratory infections, dental disease, or hormonal changes (pregnancy). The use of complementary liquid chromatography platforms, with hydrophilic interaction chromatography and reverse-phased columns, allowed us to detect a wide spectrum of EBC biomarker compounds that could be related to these health alterations. Moreover, these two analytical techniques not only provided complementary metabolite information but in both cases they also provided promising diagnostic information for these health conditions.
Graphical abstract
Collection of the exhaled condensed breath from a bottlenose dolphin from U.S. Navy Marine Mammal Program (MMP)</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-017-0581-6</identifier><identifier>PMID: 29063162</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Analytical Chemistry ; Aquatic mammals ; Biochemistry ; Biomarkers ; Blood ; Cetacea ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Chromatography ; Condensates ; Condensation (Physics) ; Diagnostic systems ; Disease ; Dolphins ; Dolphins & porpoises ; Dolphins (Mammals) ; Food Science ; Health ; Infections ; Inflammation ; Laboratory Medicine ; Liquid chromatography ; Longitudinal studies ; Mass spectrometry ; Mass spectroscopy ; Metabolites ; Metabolomics ; Monitoring/Environmental Analysis ; Observations ; Paper in Forefront ; Physiological aspects ; Pregnancy ; Respiration ; Statistical analysis ; Statistical methods ; Tursiops truncatus ; Urine ; Well being</subject><ispartof>Analytical and bioanalytical chemistry, 2017-11, Vol.409 (28), p.6523-6536</ispartof><rights>Springer-Verlag GmbH Germany 2017</rights><rights>COPYRIGHT 2017 Springer</rights><rights>Analytical and Bioanalytical Chemistry is a copyright of Springer, (2017). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c476t-c17a79ceaee982cc7f6b152ffdfe879afea3d4e1474cd3e4e9714c13584607e83</citedby><cites>FETCH-LOGICAL-c476t-c17a79ceaee982cc7f6b152ffdfe879afea3d4e1474cd3e4e9714c13584607e83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00216-017-0581-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00216-017-0581-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29063162$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Borras, Eva</creatorcontrib><creatorcontrib>Aksenov, Alexander A.</creatorcontrib><creatorcontrib>Baird, Mark</creatorcontrib><creatorcontrib>Novick, Brittany</creatorcontrib><creatorcontrib>Schivo, Michael</creatorcontrib><creatorcontrib>Zamuruyev, Konstantin O.</creatorcontrib><creatorcontrib>Pasamontes, Alberto</creatorcontrib><creatorcontrib>Parry, Celeste</creatorcontrib><creatorcontrib>Foutouhi, Soraya</creatorcontrib><creatorcontrib>Venn-Watson, Stephanie</creatorcontrib><creatorcontrib>Weimer, Bart C.</creatorcontrib><creatorcontrib>Davis, Cristina E.</creatorcontrib><title>Exhaled breath condensate methods adapted from human studies using longitudinal metabolomics for predicting early health alterations in dolphins</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>Monitoring health conditions is essential to detect early asymptomatic stages of a disease. To achieve this, blood, urine and breath samples are commonly used as a routine clinical diagnostic. These samples offer the opportunity to detect specific metabolites related to diseases and provide a better understanding of their development. Although blood samples are commonly used routinely to monitor health, the implementation of a relatively noninvasive technique, such as exhaled breath condensate (EBC) analysis, may further benefit the well-being of both humans and other animals. EBC analysis can be used to track possible physical or biochemical alterations caused by common diseases of the bottlenose dolphin (
Tursiops truncatus
), such as infections or inflammatory-mediated processes. We have used an untargeted metabolomic method with liquid chromatography–mass spectrometry analysis of EBC samples to determine biomarkers related to disease development. In this study, five dolphins under human care were followed up for 1 year. We collected paired blood, physical examination information, and EBC samples. We then statistically correlated this information to predict specific health alterations. Three dolphins provided promising case study information about biomarkers related to cutaneous infections, respiratory infections, dental disease, or hormonal changes (pregnancy). The use of complementary liquid chromatography platforms, with hydrophilic interaction chromatography and reverse-phased columns, allowed us to detect a wide spectrum of EBC biomarker compounds that could be related to these health alterations. Moreover, these two analytical techniques not only provided complementary metabolite information but in both cases they also provided promising diagnostic information for these health conditions.
Graphical abstract
Collection of the exhaled condensed breath from a bottlenose dolphin from U.S. Navy Marine Mammal Program (MMP)</description><subject>Analytical Chemistry</subject><subject>Aquatic mammals</subject><subject>Biochemistry</subject><subject>Biomarkers</subject><subject>Blood</subject><subject>Cetacea</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chromatography</subject><subject>Condensates</subject><subject>Condensation (Physics)</subject><subject>Diagnostic systems</subject><subject>Disease</subject><subject>Dolphins</subject><subject>Dolphins & porpoises</subject><subject>Dolphins (Mammals)</subject><subject>Food Science</subject><subject>Health</subject><subject>Infections</subject><subject>Inflammation</subject><subject>Laboratory Medicine</subject><subject>Liquid chromatography</subject><subject>Longitudinal studies</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Metabolites</subject><subject>Metabolomics</subject><subject>Monitoring/Environmental Analysis</subject><subject>Observations</subject><subject>Paper in Forefront</subject><subject>Physiological aspects</subject><subject>Pregnancy</subject><subject>Respiration</subject><subject>Statistical analysis</subject><subject>Statistical methods</subject><subject>Tursiops truncatus</subject><subject>Urine</subject><subject>Well 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breath condensate methods adapted from human studies using longitudinal metabolomics for predicting early health alterations in dolphins</title><author>Borras, Eva ; Aksenov, Alexander A. ; Baird, Mark ; Novick, Brittany ; Schivo, Michael ; Zamuruyev, Konstantin O. ; Pasamontes, Alberto ; Parry, Celeste ; Foutouhi, Soraya ; Venn-Watson, Stephanie ; Weimer, Bart C. ; Davis, Cristina E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c476t-c17a79ceaee982cc7f6b152ffdfe879afea3d4e1474cd3e4e9714c13584607e83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Analytical Chemistry</topic><topic>Aquatic mammals</topic><topic>Biochemistry</topic><topic>Biomarkers</topic><topic>Blood</topic><topic>Cetacea</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chromatography</topic><topic>Condensates</topic><topic>Condensation (Physics)</topic><topic>Diagnostic systems</topic><topic>Disease</topic><topic>Dolphins</topic><topic>Dolphins & porpoises</topic><topic>Dolphins (Mammals)</topic><topic>Food Science</topic><topic>Health</topic><topic>Infections</topic><topic>Inflammation</topic><topic>Laboratory Medicine</topic><topic>Liquid chromatography</topic><topic>Longitudinal studies</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Metabolites</topic><topic>Metabolomics</topic><topic>Monitoring/Environmental Analysis</topic><topic>Observations</topic><topic>Paper in Forefront</topic><topic>Physiological aspects</topic><topic>Pregnancy</topic><topic>Respiration</topic><topic>Statistical analysis</topic><topic>Statistical methods</topic><topic>Tursiops truncatus</topic><topic>Urine</topic><topic>Well 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dolphins</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2017-11-01</date><risdate>2017</risdate><volume>409</volume><issue>28</issue><spage>6523</spage><epage>6536</epage><pages>6523-6536</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>Monitoring health conditions is essential to detect early asymptomatic stages of a disease. To achieve this, blood, urine and breath samples are commonly used as a routine clinical diagnostic. These samples offer the opportunity to detect specific metabolites related to diseases and provide a better understanding of their development. Although blood samples are commonly used routinely to monitor health, the implementation of a relatively noninvasive technique, such as exhaled breath condensate (EBC) analysis, may further benefit the well-being of both humans and other animals. EBC analysis can be used to track possible physical or biochemical alterations caused by common diseases of the bottlenose dolphin (
Tursiops truncatus
), such as infections or inflammatory-mediated processes. We have used an untargeted metabolomic method with liquid chromatography–mass spectrometry analysis of EBC samples to determine biomarkers related to disease development. In this study, five dolphins under human care were followed up for 1 year. We collected paired blood, physical examination information, and EBC samples. We then statistically correlated this information to predict specific health alterations. Three dolphins provided promising case study information about biomarkers related to cutaneous infections, respiratory infections, dental disease, or hormonal changes (pregnancy). The use of complementary liquid chromatography platforms, with hydrophilic interaction chromatography and reverse-phased columns, allowed us to detect a wide spectrum of EBC biomarker compounds that could be related to these health alterations. Moreover, these two analytical techniques not only provided complementary metabolite information but in both cases they also provided promising diagnostic information for these health conditions.
Graphical abstract
Collection of the exhaled condensed breath from a bottlenose dolphin from U.S. Navy Marine Mammal Program (MMP)</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>29063162</pmid><doi>10.1007/s00216-017-0581-6</doi><tpages>14</tpages></addata></record> |
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| identifier | ISSN: 1618-2642 |
| ispartof | Analytical and bioanalytical chemistry, 2017-11, Vol.409 (28), p.6523-6536 |
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| language | eng |
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| source | SpringerNature Journals |
| subjects | Analytical Chemistry Aquatic mammals Biochemistry Biomarkers Blood Cetacea Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Chromatography Condensates Condensation (Physics) Diagnostic systems Disease Dolphins Dolphins & porpoises Dolphins (Mammals) Food Science Health Infections Inflammation Laboratory Medicine Liquid chromatography Longitudinal studies Mass spectrometry Mass spectroscopy Metabolites Metabolomics Monitoring/Environmental Analysis Observations Paper in Forefront Physiological aspects Pregnancy Respiration Statistical analysis Statistical methods Tursiops truncatus Urine Well being |
| title | Exhaled breath condensate methods adapted from human studies using longitudinal metabolomics for predicting early health alterations in dolphins |
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