Evaluation of the spatial arrangement of Purkinje cells in ataxic rat's cerebellum after Sertoli cells transplantation
Purkinje cells (PCs) pathology is important in cerebellar disorders like ataxia. The spatial arrangement of PCs after different treatments has not been studied extensively. Immunohistochemistry (IHC) analysis of cerebellum can give a proper tool for explaining the pathophysiology of PCs in ataxia. H...
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| Veröffentlicht in: | Folia morphologica 2018-01, Vol.77 (2), p.194-200 |
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| creator | Mohammadi, R Heidari, M H Sadeghi, Y Abdollahifar, M A Aghaei, A |
| description | Purkinje cells (PCs) pathology is important in cerebellar disorders like ataxia. The spatial arrangement of PCs after different treatments has not been studied extensively. Immunohistochemistry (IHC) analysis of cerebellum can give a proper tool for explaining the pathophysiology of PCs in ataxia. Here we stereologically analysed the 3-dimensional spatial arrangement of PCs in the cerebellum of rats after ataxia induction with 3-acetylpyridine (3-AP).
Ataxia was induced in rats by intraperitoneal injection of 3-AP (65 mg/kg). Spatial arrangement of PCs for differences in ataxic rats with (3-AP-SC) or without (3-AP) Sertoli cells (SCs) transplantation was evaluated using second-order stereology. The IHC method by using antibodies to anti-calbindin in the cerebellum was applied.
Our results showed that a random arrangement is at larger distances between PCs in 3-AP and 3-Ap-SC groups. Therefore the PCs are not normally arranged after 3-AP and SCs transplantation stored the spatial arrangements of the cells after ataxia induction in rats. IHC analyse shows that number of PCs was significantly improved after the SC transplantation.
Segregation of PCs can be observed at some areas in the ataxic rats' cerebellum. However, the spatial arrangement of PCs was unchanged in SCs transplanted rats. (Folia Morphol 2018; 77, 2: 194-200). |
| doi_str_mv | 10.5603/FM.a2017.0091 |
| format | Article |
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Ataxia was induced in rats by intraperitoneal injection of 3-AP (65 mg/kg). Spatial arrangement of PCs for differences in ataxic rats with (3-AP-SC) or without (3-AP) Sertoli cells (SCs) transplantation was evaluated using second-order stereology. The IHC method by using antibodies to anti-calbindin in the cerebellum was applied.
Our results showed that a random arrangement is at larger distances between PCs in 3-AP and 3-Ap-SC groups. Therefore the PCs are not normally arranged after 3-AP and SCs transplantation stored the spatial arrangements of the cells after ataxia induction in rats. IHC analyse shows that number of PCs was significantly improved after the SC transplantation.
Segregation of PCs can be observed at some areas in the ataxic rats' cerebellum. However, the spatial arrangement of PCs was unchanged in SCs transplanted rats. (Folia Morphol 2018; 77, 2: 194-200).</description><identifier>ISSN: 0015-5659</identifier><identifier>EISSN: 1644-3284</identifier><identifier>DOI: 10.5603/FM.a2017.0091</identifier><identifier>PMID: 29064552</identifier><language>eng</language><publisher>Poland: Wydawnictwo Via Medica</publisher><subject>Animals ; Ataxia ; Calbindin ; Cerebellar Ataxia - chemically induced ; Cerebellar Ataxia - metabolism ; Cerebellar Ataxia - pathology ; Cerebellar Ataxia - therapy ; Cerebellum ; Cerebellum - metabolism ; Cerebellum - pathology ; Immunohistochemistry ; Male ; Motor Activity - drug effects ; Purkinje cells ; Purkinje Cells - metabolism ; Purkinje Cells - pathology ; Pyridines - toxicity ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Rats, Wistar ; Sertoli cells ; Sertoli Cells - metabolism ; Sertoli Cells - pathology ; Sertoli Cells - transplantation ; Transplantation</subject><ispartof>Folia morphologica, 2018-01, Vol.77 (2), p.194-200</ispartof><rights>2018. This work is published under https://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c360t-39a5169715b9180fea4a6fee6160da5dd5ec4f9ac444e61bfd653bd883043f483</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29064552$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mohammadi, R</creatorcontrib><creatorcontrib>Heidari, M H</creatorcontrib><creatorcontrib>Sadeghi, Y</creatorcontrib><creatorcontrib>Abdollahifar, M A</creatorcontrib><creatorcontrib>Aghaei, A</creatorcontrib><title>Evaluation of the spatial arrangement of Purkinje cells in ataxic rat's cerebellum after Sertoli cells transplantation</title><title>Folia morphologica</title><addtitle>Folia Morphol (Warsz)</addtitle><description>Purkinje cells (PCs) pathology is important in cerebellar disorders like ataxia. The spatial arrangement of PCs after different treatments has not been studied extensively. Immunohistochemistry (IHC) analysis of cerebellum can give a proper tool for explaining the pathophysiology of PCs in ataxia. Here we stereologically analysed the 3-dimensional spatial arrangement of PCs in the cerebellum of rats after ataxia induction with 3-acetylpyridine (3-AP).
Ataxia was induced in rats by intraperitoneal injection of 3-AP (65 mg/kg). Spatial arrangement of PCs for differences in ataxic rats with (3-AP-SC) or without (3-AP) Sertoli cells (SCs) transplantation was evaluated using second-order stereology. The IHC method by using antibodies to anti-calbindin in the cerebellum was applied.
Our results showed that a random arrangement is at larger distances between PCs in 3-AP and 3-Ap-SC groups. Therefore the PCs are not normally arranged after 3-AP and SCs transplantation stored the spatial arrangements of the cells after ataxia induction in rats. IHC analyse shows that number of PCs was significantly improved after the SC transplantation.
Segregation of PCs can be observed at some areas in the ataxic rats' cerebellum. However, the spatial arrangement of PCs was unchanged in SCs transplanted rats. (Folia Morphol 2018; 77, 2: 194-200).</description><subject>Animals</subject><subject>Ataxia</subject><subject>Calbindin</subject><subject>Cerebellar Ataxia - chemically induced</subject><subject>Cerebellar Ataxia - metabolism</subject><subject>Cerebellar Ataxia - pathology</subject><subject>Cerebellar Ataxia - therapy</subject><subject>Cerebellum</subject><subject>Cerebellum - metabolism</subject><subject>Cerebellum - pathology</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Motor Activity - drug effects</subject><subject>Purkinje cells</subject><subject>Purkinje Cells - metabolism</subject><subject>Purkinje Cells - pathology</subject><subject>Pyridines - toxicity</subject><subject>Random Allocation</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Rats, Wistar</subject><subject>Sertoli cells</subject><subject>Sertoli Cells - metabolism</subject><subject>Sertoli Cells - pathology</subject><subject>Sertoli Cells - transplantation</subject><subject>Transplantation</subject><issn>0015-5659</issn><issn>1644-3284</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpdkc1P3DAQxS3UChbKkSuy1EO5ZBnHHxsfEWKhEggk6NmaJOM2Sz4W20Htf08C2x56Gs28n9486TF2ImCpDcjz9d0ScxCrJYAVe2whjFKZzAv1iS0AhM600faAHca4AdBKrWCfHeQWjNI6X7DXq1dsR0zN0PPB8_SLeNxOK7YcQ8D-J3XUp1l6GMNz02-IV9S2kTc9x4S_m4oHTN_idA1UTsrYcfSJAn-kkIa22eFp8orbFvv0_usL--yxjXS8m0fsx_rq6fImu72__n55cZtV0kDKpEUtjF0JXVpRgCdUaDyREQZq1HWtqVLeYqWUmo6lr42WZV0UEpT0qpBH7OzDdxuGl5Ficl0T50TY0zBGJ6zWYAorVhP69T90M4yhn9K5XBmVCyFgprIPqgpDjIG824amw_DHCXBzIW59594LcXMhE3-6cx3Ljup_9N8G5Bt_BIcp</recordid><startdate>20180101</startdate><enddate>20180101</enddate><creator>Mohammadi, R</creator><creator>Heidari, M H</creator><creator>Sadeghi, Y</creator><creator>Abdollahifar, M A</creator><creator>Aghaei, A</creator><general>Wydawnictwo Via Medica</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H9R</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20180101</creationdate><title>Evaluation of the spatial arrangement of Purkinje cells in ataxic rat's cerebellum after Sertoli cells transplantation</title><author>Mohammadi, R ; Heidari, M H ; Sadeghi, Y ; Abdollahifar, M A ; Aghaei, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-39a5169715b9180fea4a6fee6160da5dd5ec4f9ac444e61bfd653bd883043f483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Ataxia</topic><topic>Calbindin</topic><topic>Cerebellar Ataxia - chemically induced</topic><topic>Cerebellar Ataxia - metabolism</topic><topic>Cerebellar Ataxia - pathology</topic><topic>Cerebellar Ataxia - therapy</topic><topic>Cerebellum</topic><topic>Cerebellum - metabolism</topic><topic>Cerebellum - pathology</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Motor Activity - drug effects</topic><topic>Purkinje cells</topic><topic>Purkinje Cells - metabolism</topic><topic>Purkinje Cells - pathology</topic><topic>Pyridines - toxicity</topic><topic>Random Allocation</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Rats, Wistar</topic><topic>Sertoli cells</topic><topic>Sertoli Cells - metabolism</topic><topic>Sertoli Cells - pathology</topic><topic>Sertoli Cells - transplantation</topic><topic>Transplantation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mohammadi, R</creatorcontrib><creatorcontrib>Heidari, M H</creatorcontrib><creatorcontrib>Sadeghi, Y</creatorcontrib><creatorcontrib>Abdollahifar, M A</creatorcontrib><creatorcontrib>Aghaei, A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Illustrata: Natural Sciences</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Folia morphologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mohammadi, R</au><au>Heidari, M H</au><au>Sadeghi, Y</au><au>Abdollahifar, M A</au><au>Aghaei, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of the spatial arrangement of Purkinje cells in ataxic rat's cerebellum after Sertoli cells transplantation</atitle><jtitle>Folia morphologica</jtitle><addtitle>Folia Morphol (Warsz)</addtitle><date>2018-01-01</date><risdate>2018</risdate><volume>77</volume><issue>2</issue><spage>194</spage><epage>200</epage><pages>194-200</pages><issn>0015-5659</issn><eissn>1644-3284</eissn><abstract>Purkinje cells (PCs) pathology is important in cerebellar disorders like ataxia. The spatial arrangement of PCs after different treatments has not been studied extensively. Immunohistochemistry (IHC) analysis of cerebellum can give a proper tool for explaining the pathophysiology of PCs in ataxia. Here we stereologically analysed the 3-dimensional spatial arrangement of PCs in the cerebellum of rats after ataxia induction with 3-acetylpyridine (3-AP).
Ataxia was induced in rats by intraperitoneal injection of 3-AP (65 mg/kg). Spatial arrangement of PCs for differences in ataxic rats with (3-AP-SC) or without (3-AP) Sertoli cells (SCs) transplantation was evaluated using second-order stereology. The IHC method by using antibodies to anti-calbindin in the cerebellum was applied.
Our results showed that a random arrangement is at larger distances between PCs in 3-AP and 3-Ap-SC groups. Therefore the PCs are not normally arranged after 3-AP and SCs transplantation stored the spatial arrangements of the cells after ataxia induction in rats. IHC analyse shows that number of PCs was significantly improved after the SC transplantation.
Segregation of PCs can be observed at some areas in the ataxic rats' cerebellum. However, the spatial arrangement of PCs was unchanged in SCs transplanted rats. (Folia Morphol 2018; 77, 2: 194-200).</abstract><cop>Poland</cop><pub>Wydawnictwo Via Medica</pub><pmid>29064552</pmid><doi>10.5603/FM.a2017.0091</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Ataxia Calbindin Cerebellar Ataxia - chemically induced Cerebellar Ataxia - metabolism Cerebellar Ataxia - pathology Cerebellar Ataxia - therapy Cerebellum Cerebellum - metabolism Cerebellum - pathology Immunohistochemistry Male Motor Activity - drug effects Purkinje cells Purkinje Cells - metabolism Purkinje Cells - pathology Pyridines - toxicity Random Allocation Rats Rats, Sprague-Dawley Rats, Wistar Sertoli cells Sertoli Cells - metabolism Sertoli Cells - pathology Sertoli Cells - transplantation Transplantation |
| title | Evaluation of the spatial arrangement of Purkinje cells in ataxic rat's cerebellum after Sertoli cells transplantation |
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