Identification of wheat gliadins as an allergen family related to baker's asthma
Background Flour is still one of the most common causes of occupational asthma worldwide. Thus far, little is known about the relevant allergens causing baker's asthma. Therefore the reliability of current diagnostic procedures is insufficient. Only few of the suspected causative wheat allergen...
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| Veröffentlicht in: | Journal of allergy and clinical immunology 2008-03, Vol.121 (3), p.744-749 |
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| description | Background Flour is still one of the most common causes of occupational asthma worldwide. Thus far, little is known about the relevant allergens causing baker's asthma. Therefore the reliability of current diagnostic procedures is insufficient. Only few of the suspected causative wheat allergens have been hitherto characterized on the molecular level. Objective The aim was to identify and characterize unknown wheat allergens related to baker's asthma to improve the reliability of diagnostic procedures. Methods A wheat pJuFo cDNA phage display library was created and screened for IgE binding to wheat proteins with pooled sera from patients with baker's asthma. After identifying an αβ-gliadin, the frequency of sensitization was investigated by means of ELISA screening of 153 bakers' sera with the recombinant αβ-gliadin. Furthermore, the allergenicity of native total gliadin (αβ, γ, ω) was analyzed by means of ImmunoCAP. Results One cDNA clone was identified as an αβ-gliadin. Serum IgE antibodies to the recombinant allergen were found in 12% of bakers with occupational asthma. Of the asthmatic bakers, 33% showed sensitization to native total gliadin; 4% of them had negative results on routine IgE testing with wheat extract. Conclusions Gliadins represent a newly discovered family of inhalable allergens in baker's asthma. This finding demonstrates that water-insoluble proteins might also represent causative allergens. |
| doi_str_mv | 10.1016/j.jaci.2007.09.051 |
| format | Article |
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Thus far, little is known about the relevant allergens causing baker's asthma. Therefore the reliability of current diagnostic procedures is insufficient. Only few of the suspected causative wheat allergens have been hitherto characterized on the molecular level. Objective The aim was to identify and characterize unknown wheat allergens related to baker's asthma to improve the reliability of diagnostic procedures. Methods A wheat pJuFo cDNA phage display library was created and screened for IgE binding to wheat proteins with pooled sera from patients with baker's asthma. After identifying an αβ-gliadin, the frequency of sensitization was investigated by means of ELISA screening of 153 bakers' sera with the recombinant αβ-gliadin. Furthermore, the allergenicity of native total gliadin (αβ, γ, ω) was analyzed by means of ImmunoCAP. Results One cDNA clone was identified as an αβ-gliadin. Serum IgE antibodies to the recombinant allergen were found in 12% of bakers with occupational asthma. Of the asthmatic bakers, 33% showed sensitization to native total gliadin; 4% of them had negative results on routine IgE testing with wheat extract. Conclusions Gliadins represent a newly discovered family of inhalable allergens in baker's asthma. This finding demonstrates that water-insoluble proteins might also represent causative allergens.</description><identifier>ISSN: 0091-6749</identifier><identifier>EISSN: 1097-6825</identifier><identifier>DOI: 10.1016/j.jaci.2007.09.051</identifier><identifier>PMID: 18036646</identifier><identifier>CODEN: JACIBY</identifier><language>eng</language><publisher>New York, NY: Mosby, Inc</publisher><subject>Adult ; Allergens - immunology ; Allergies ; Allergy and Immunology ; Aqueous solutions ; Asthma - blood ; Asthma - diagnosis ; Asthma - immunology ; Baker's asthma ; Binding sites ; Biological and medical sciences ; cDNA phage display ; Cloning, Molecular ; Electrophoresis, Polyacrylamide Gel ; Enzyme-Linked Immunosorbent Assay ; Female ; Food ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Gliadin - immunology ; gliadins ; Humans ; Immunoglobulin E - blood ; Immunoglobulin E - immunology ; Libraries ; Male ; Medical sciences ; Occupational Diseases - blood ; Occupational Diseases - diagnosis ; Occupational Diseases - immunology ; Occupational Exposure ; Peptide Library ; Plasmids ; Polymerase Chain Reaction ; Proteins ; recombinant allergens ; Recombinant Proteins - immunology ; recombinant αβ-gliadin ; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis ; Skin ; Triticum - immunology ; Triticum aestivum ; wheat ; Wheat Hypersensitivity - immunology</subject><ispartof>Journal of allergy and clinical immunology, 2008-03, Vol.121 (3), p.744-749</ispartof><rights>American Academy of Allergy, Asthma & Immunology</rights><rights>2008 American Academy of Allergy, Asthma & Immunology</rights><rights>2008 INIST-CNRS</rights><rights>Copyright Elsevier Limited Mar 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c626t-243da7be0497b6a7831236c63dca179ae88662821c7c5e4a3916aed3d3ecfb143</citedby><cites>FETCH-LOGICAL-c626t-243da7be0497b6a7831236c63dca179ae88662821c7c5e4a3916aed3d3ecfb143</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jaci.2007.09.051$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20664346$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18036646$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bittner, Cordula, MD</creatorcontrib><creatorcontrib>Grassau, Britta, MS</creatorcontrib><creatorcontrib>Frenzel, Karsten, PhD</creatorcontrib><creatorcontrib>Baur, Xaver, MD</creatorcontrib><title>Identification of wheat gliadins as an allergen family related to baker's asthma</title><title>Journal of allergy and clinical immunology</title><addtitle>J Allergy Clin Immunol</addtitle><description>Background Flour is still one of the most common causes of occupational asthma worldwide. Thus far, little is known about the relevant allergens causing baker's asthma. Therefore the reliability of current diagnostic procedures is insufficient. Only few of the suspected causative wheat allergens have been hitherto characterized on the molecular level. Objective The aim was to identify and characterize unknown wheat allergens related to baker's asthma to improve the reliability of diagnostic procedures. Methods A wheat pJuFo cDNA phage display library was created and screened for IgE binding to wheat proteins with pooled sera from patients with baker's asthma. After identifying an αβ-gliadin, the frequency of sensitization was investigated by means of ELISA screening of 153 bakers' sera with the recombinant αβ-gliadin. Furthermore, the allergenicity of native total gliadin (αβ, γ, ω) was analyzed by means of ImmunoCAP. Results One cDNA clone was identified as an αβ-gliadin. Serum IgE antibodies to the recombinant allergen were found in 12% of bakers with occupational asthma. Of the asthmatic bakers, 33% showed sensitization to native total gliadin; 4% of them had negative results on routine IgE testing with wheat extract. Conclusions Gliadins represent a newly discovered family of inhalable allergens in baker's asthma. This finding demonstrates that water-insoluble proteins might also represent causative allergens.</description><subject>Adult</subject><subject>Allergens - immunology</subject><subject>Allergies</subject><subject>Allergy and Immunology</subject><subject>Aqueous solutions</subject><subject>Asthma - blood</subject><subject>Asthma - diagnosis</subject><subject>Asthma - immunology</subject><subject>Baker's asthma</subject><subject>Binding sites</subject><subject>Biological and medical sciences</subject><subject>cDNA phage display</subject><subject>Cloning, Molecular</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Food</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Gliadin - immunology</subject><subject>gliadins</subject><subject>Humans</subject><subject>Immunoglobulin E - blood</subject><subject>Immunoglobulin E - immunology</subject><subject>Libraries</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Occupational Diseases - blood</subject><subject>Occupational Diseases - diagnosis</subject><subject>Occupational Diseases - immunology</subject><subject>Occupational Exposure</subject><subject>Peptide Library</subject><subject>Plasmids</subject><subject>Polymerase Chain Reaction</subject><subject>Proteins</subject><subject>recombinant allergens</subject><subject>Recombinant Proteins - immunology</subject><subject>recombinant αβ-gliadin</subject><subject>Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis</subject><subject>Skin</subject><subject>Triticum - immunology</subject><subject>Triticum aestivum</subject><subject>wheat</subject><subject>Wheat Hypersensitivity - immunology</subject><issn>0091-6749</issn><issn>1097-6825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0m1r1TAUB_AgirtOv4AvpCDqq9Y8tEkDIozhw2CgoILvwml6uqVL05n0Tu63N_VeHOyFDAIh8DshJ_9DyHNGK0aZfDtWI1hXcUpVRXVFG_aAbBjVqpQtbx6SDaWalVLV-og8SWmk-Sxa_ZgcsZYKKWu5IV_PegyLG5yFxc2hmIfi9yXCUlx4B70LqYC8QgHeY7zAUAwwOb8rInpYsC-WuejgCuObFS6XEzwljwbwCZ8d9mPy4-OH76efy_Mvn85OT85LK7lcSl6LHlSHtNaqk6BawbiQVoreAlMasG2l5C1nVtkGaxCaScBe9ALt0LFaHJPX-3uv4_xri2kxk0sWvYeA8zYZpptaSa7uAetWCi3uBZmUTYYv78Bx3saQuzWsobVqldI0K75XNs4pRRzMdXQTxJ1h1Kz5mdGs-Zk1P0O1yfnloheHq7fdhP1tySGwDF4dACQLfogQrEv_HKdZib_u3d5hzuDGYTTJOgwWexfRLqaf3f_f8f5OufUu5CHxV7jDdNuvSdxQ822dtHXQqKL5m-RP8Qd99cvH</recordid><startdate>20080301</startdate><enddate>20080301</enddate><creator>Bittner, Cordula, MD</creator><creator>Grassau, Britta, MS</creator><creator>Frenzel, Karsten, PhD</creator><creator>Baur, Xaver, MD</creator><general>Mosby, Inc</general><general>Elsevier</general><general>Elsevier Limited</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7T5</scope><scope>H94</scope><scope>K9.</scope><scope>NAPCQ</scope></search><sort><creationdate>20080301</creationdate><title>Identification of wheat gliadins as an allergen family related to baker's asthma</title><author>Bittner, Cordula, MD ; Grassau, Britta, MS ; Frenzel, Karsten, PhD ; Baur, Xaver, MD</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c626t-243da7be0497b6a7831236c63dca179ae88662821c7c5e4a3916aed3d3ecfb143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Adult</topic><topic>Allergens - immunology</topic><topic>Allergies</topic><topic>Allergy and Immunology</topic><topic>Aqueous solutions</topic><topic>Asthma - blood</topic><topic>Asthma - diagnosis</topic><topic>Asthma - immunology</topic><topic>Baker's asthma</topic><topic>Binding sites</topic><topic>Biological and medical sciences</topic><topic>cDNA phage display</topic><topic>Cloning, Molecular</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Food</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Gliadin - immunology</topic><topic>gliadins</topic><topic>Humans</topic><topic>Immunoglobulin E - blood</topic><topic>Immunoglobulin E - immunology</topic><topic>Libraries</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Occupational Diseases - blood</topic><topic>Occupational Diseases - diagnosis</topic><topic>Occupational Diseases - immunology</topic><topic>Occupational Exposure</topic><topic>Peptide Library</topic><topic>Plasmids</topic><topic>Polymerase Chain Reaction</topic><topic>Proteins</topic><topic>recombinant allergens</topic><topic>Recombinant Proteins - immunology</topic><topic>recombinant αβ-gliadin</topic><topic>Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis</topic><topic>Skin</topic><topic>Triticum - immunology</topic><topic>Triticum aestivum</topic><topic>wheat</topic><topic>Wheat Hypersensitivity - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bittner, Cordula, MD</creatorcontrib><creatorcontrib>Grassau, Britta, MS</creatorcontrib><creatorcontrib>Frenzel, Karsten, PhD</creatorcontrib><creatorcontrib>Baur, Xaver, MD</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><jtitle>Journal of allergy and clinical immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bittner, Cordula, MD</au><au>Grassau, Britta, MS</au><au>Frenzel, Karsten, PhD</au><au>Baur, Xaver, MD</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of wheat gliadins as an allergen family related to baker's asthma</atitle><jtitle>Journal of allergy and clinical immunology</jtitle><addtitle>J Allergy Clin Immunol</addtitle><date>2008-03-01</date><risdate>2008</risdate><volume>121</volume><issue>3</issue><spage>744</spage><epage>749</epage><pages>744-749</pages><issn>0091-6749</issn><eissn>1097-6825</eissn><coden>JACIBY</coden><abstract>Background Flour is still one of the most common causes of occupational asthma worldwide. Thus far, little is known about the relevant allergens causing baker's asthma. Therefore the reliability of current diagnostic procedures is insufficient. Only few of the suspected causative wheat allergens have been hitherto characterized on the molecular level. Objective The aim was to identify and characterize unknown wheat allergens related to baker's asthma to improve the reliability of diagnostic procedures. Methods A wheat pJuFo cDNA phage display library was created and screened for IgE binding to wheat proteins with pooled sera from patients with baker's asthma. After identifying an αβ-gliadin, the frequency of sensitization was investigated by means of ELISA screening of 153 bakers' sera with the recombinant αβ-gliadin. Furthermore, the allergenicity of native total gliadin (αβ, γ, ω) was analyzed by means of ImmunoCAP. Results One cDNA clone was identified as an αβ-gliadin. Serum IgE antibodies to the recombinant allergen were found in 12% of bakers with occupational asthma. Of the asthmatic bakers, 33% showed sensitization to native total gliadin; 4% of them had negative results on routine IgE testing with wheat extract. Conclusions Gliadins represent a newly discovered family of inhalable allergens in baker's asthma. This finding demonstrates that water-insoluble proteins might also represent causative allergens.</abstract><cop>New York, NY</cop><pub>Mosby, Inc</pub><pmid>18036646</pmid><doi>10.1016/j.jaci.2007.09.051</doi><tpages>6</tpages></addata></record> |
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| subjects | Adult Allergens - immunology Allergies Allergy and Immunology Aqueous solutions Asthma - blood Asthma - diagnosis Asthma - immunology Baker's asthma Binding sites Biological and medical sciences cDNA phage display Cloning, Molecular Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Female Food Fundamental and applied biological sciences. Psychology Fundamental immunology Gliadin - immunology gliadins Humans Immunoglobulin E - blood Immunoglobulin E - immunology Libraries Male Medical sciences Occupational Diseases - blood Occupational Diseases - diagnosis Occupational Diseases - immunology Occupational Exposure Peptide Library Plasmids Polymerase Chain Reaction Proteins recombinant allergens Recombinant Proteins - immunology recombinant αβ-gliadin Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis Skin Triticum - immunology Triticum aestivum wheat Wheat Hypersensitivity - immunology |
| title | Identification of wheat gliadins as an allergen family related to baker's asthma |
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