Influence of exposure time on the release of bacteria from a biofilm on Ti6Al4V discs using sonication: An in vitro model
Implant sonication is considered a useful method for the diagnosis of implant-related infections. We designed an in vitro study using Ti6Al4V discs and 5 different bacteria to determine the optimal sonication time for recovery of most bacteria tested to enable use of sonication in clinical practice...
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| Veröffentlicht in: | Diagnostic microbiology and infectious disease 2017-12, Vol.89 (4), p.258-261 |
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| creator | Prieto-Borja, Laura Conde, Ana Arenas, María A. de Damborenea, Juan J. Esteban, Jaime |
| description | Implant sonication is considered a useful method for the diagnosis of implant-related infections. We designed an in vitro study using Ti6Al4V discs and 5 different bacteria to determine the optimal sonication time for recovery of most bacteria tested to enable use of sonication in clinical practice for microbiological diagnosis of implant-related infections. We carried out a specific protocol for the adherence and subsequent biofilm formation on the materials used. The discs were then sonicated and the retrieved bacteria were quantified. From minute 1 to 5, the amount of recovered organisms grew progressively for all bacteria. Between minute 6 and minute 10, the number was irregular for all strains except E. coli, though no pattern was evidenced. E. coli was the only microorganism with a progressive increase in liberation throughout the process. Significant differences were observed in each of the 10minutes analyzed as concerns the release of the 5 strains (P |
| doi_str_mv | 10.1016/j.diagmicrobio.2017.08.018 |
| format | Article |
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Considering that infections in which biofilms are involved could be polymicrobial, we concluded that 5minutes is the optimal time of sonication in order to recover the maximum amount of most bacteria attached to Ti6Al4V discs.
•An in vitro trial using Ti discs and 5 different bacterial strains is proposed.•The study includes biofilm formation and exposure to ultrasound of samples.•The optimal sonication time to recover the maximum bacteria attached is 5minutes.•Gram-positive strains have higher release from the discs than gram negatives.</description><identifier>ISSN: 0732-8893</identifier><identifier>EISSN: 1879-0070</identifier><identifier>DOI: 10.1016/j.diagmicrobio.2017.08.018</identifier><identifier>PMID: 29037465</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject><![CDATA[Biofilm ; Biofilms - growth & development ; Bioimplants ; Colony Count, Microbial ; Enterococcus faecalis - growth & development ; Enterococcus faecalis - isolation & purification ; Escherichia coli - growth & development ; Escherichia coli - isolation & purification ; Prostheses and Implants ; Prosthesis-Related Infections ; Pseudomonas aeruginosa - growth & development ; Pseudomonas aeruginosa - isolation & purification ; Release of bacteria ; Sonication ; Staphylococcus aureus - growth & development ; Staphylococcus aureus - isolation & purification ; Staphylococcus epidermidis - growth & development ; Staphylococcus epidermidis - isolation & purification ; Ti6Al4V ; Time ; Time Factors ; Titanium - chemistry]]></subject><ispartof>Diagnostic microbiology and infectious disease, 2017-12, Vol.89 (4), p.258-261</ispartof><rights>2017 Elsevier Inc.</rights><rights>Copyright © 2017 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c380t-5c643f07be367c1010a78ae59b85bea144bd773d1b916481d5911c22833668863</citedby><cites>FETCH-LOGICAL-c380t-5c643f07be367c1010a78ae59b85bea144bd773d1b916481d5911c22833668863</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0732889317302705$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29037465$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Prieto-Borja, Laura</creatorcontrib><creatorcontrib>Conde, Ana</creatorcontrib><creatorcontrib>Arenas, María A.</creatorcontrib><creatorcontrib>de Damborenea, Juan J.</creatorcontrib><creatorcontrib>Esteban, Jaime</creatorcontrib><title>Influence of exposure time on the release of bacteria from a biofilm on Ti6Al4V discs using sonication: An in vitro model</title><title>Diagnostic microbiology and infectious disease</title><addtitle>Diagn Microbiol Infect Dis</addtitle><description>Implant sonication is considered a useful method for the diagnosis of implant-related infections. We designed an in vitro study using Ti6Al4V discs and 5 different bacteria to determine the optimal sonication time for recovery of most bacteria tested to enable use of sonication in clinical practice for microbiological diagnosis of implant-related infections. We carried out a specific protocol for the adherence and subsequent biofilm formation on the materials used. The discs were then sonicated and the retrieved bacteria were quantified. From minute 1 to 5, the amount of recovered organisms grew progressively for all bacteria. Between minute 6 and minute 10, the number was irregular for all strains except E. coli, though no pattern was evidenced. E. coli was the only microorganism with a progressive increase in liberation throughout the process. Significant differences were observed in each of the 10minutes analyzed as concerns the release of the 5 strains (P<0.021) as well as in the mean dislodgement (of the 10minutes) of all tested strains (P<0.00001).
Considering that infections in which biofilms are involved could be polymicrobial, we concluded that 5minutes is the optimal time of sonication in order to recover the maximum amount of most bacteria attached to Ti6Al4V discs.
•An in vitro trial using Ti discs and 5 different bacterial strains is proposed.•The study includes biofilm formation and exposure to ultrasound of samples.•The optimal sonication time to recover the maximum bacteria attached is 5minutes.•Gram-positive strains have higher release from the discs than gram negatives.</description><subject>Biofilm</subject><subject>Biofilms - growth & development</subject><subject>Bioimplants</subject><subject>Colony Count, Microbial</subject><subject>Enterococcus faecalis - growth & development</subject><subject>Enterococcus faecalis - isolation & purification</subject><subject>Escherichia coli - growth & development</subject><subject>Escherichia coli - isolation & purification</subject><subject>Prostheses and Implants</subject><subject>Prosthesis-Related Infections</subject><subject>Pseudomonas aeruginosa - growth & development</subject><subject>Pseudomonas aeruginosa - isolation & purification</subject><subject>Release of bacteria</subject><subject>Sonication</subject><subject>Staphylococcus aureus - growth & development</subject><subject>Staphylococcus aureus - isolation & purification</subject><subject>Staphylococcus epidermidis - growth & development</subject><subject>Staphylococcus epidermidis - isolation & purification</subject><subject>Ti6Al4V</subject><subject>Time</subject><subject>Time Factors</subject><subject>Titanium - chemistry</subject><issn>0732-8893</issn><issn>1879-0070</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMFu1DAQhi1ERbeFV0AWJy4JduzYTm-rUkqlSr0UrpbjTMqsEnuxk4q-fb1sQRw5WRp_M__MR8gHzmrOuPq0qwd0DzP6FHuMdcO4rpmpGTevyIYb3VWMafaabJgWTWVMJ07JWc47xnjTSfaGnDYdE1qqdkOebsI4rRA80DhS-LWPeU1AF5xLIdDlB9AEE7j8-793foGEjo4pztTREj_iNB_Ie1TbSX6nA2af6ZoxPNAcA3q3YAwXdBsoBvqIS4p0jgNMb8nJ6KYM717ec_Lty9X95dfq9u765nJ7W3lh2FK1XkkxMt2DUNqX-5nTxkHb9abtwXEp-0FrMfC-40oaPrQd575pjBBKGaPEOfl4nLtP8ecKebFzWRGmyQWIa7a8axvOuZayoBdHtJjNOcFo9wlnl54sZ_ag3u7sv-rtQb1lxhb1pfn9S87azzD8bf3jugCfjwCUax8Rks0eD-YHTOAXO0T8n5xnG1CbSg</recordid><startdate>201712</startdate><enddate>201712</enddate><creator>Prieto-Borja, Laura</creator><creator>Conde, Ana</creator><creator>Arenas, María A.</creator><creator>de Damborenea, Juan J.</creator><creator>Esteban, Jaime</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201712</creationdate><title>Influence of exposure time on the release of bacteria from a biofilm on Ti6Al4V discs using sonication: An in vitro model</title><author>Prieto-Borja, Laura ; Conde, Ana ; Arenas, María A. ; de Damborenea, Juan J. ; Esteban, Jaime</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c380t-5c643f07be367c1010a78ae59b85bea144bd773d1b916481d5911c22833668863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Biofilm</topic><topic>Biofilms - growth & development</topic><topic>Bioimplants</topic><topic>Colony Count, Microbial</topic><topic>Enterococcus faecalis - growth & development</topic><topic>Enterococcus faecalis - isolation & purification</topic><topic>Escherichia coli - growth & development</topic><topic>Escherichia coli - isolation & purification</topic><topic>Prostheses and Implants</topic><topic>Prosthesis-Related Infections</topic><topic>Pseudomonas aeruginosa - growth & development</topic><topic>Pseudomonas aeruginosa - isolation & purification</topic><topic>Release of bacteria</topic><topic>Sonication</topic><topic>Staphylococcus aureus - growth & development</topic><topic>Staphylococcus aureus - isolation & purification</topic><topic>Staphylococcus epidermidis - growth & development</topic><topic>Staphylococcus epidermidis - isolation & purification</topic><topic>Ti6Al4V</topic><topic>Time</topic><topic>Time Factors</topic><topic>Titanium - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Prieto-Borja, Laura</creatorcontrib><creatorcontrib>Conde, Ana</creatorcontrib><creatorcontrib>Arenas, María A.</creatorcontrib><creatorcontrib>de Damborenea, Juan J.</creatorcontrib><creatorcontrib>Esteban, Jaime</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Diagnostic microbiology and infectious disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Prieto-Borja, Laura</au><au>Conde, Ana</au><au>Arenas, María A.</au><au>de Damborenea, Juan J.</au><au>Esteban, Jaime</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of exposure time on the release of bacteria from a biofilm on Ti6Al4V discs using sonication: An in vitro model</atitle><jtitle>Diagnostic microbiology and infectious disease</jtitle><addtitle>Diagn Microbiol Infect Dis</addtitle><date>2017-12</date><risdate>2017</risdate><volume>89</volume><issue>4</issue><spage>258</spage><epage>261</epage><pages>258-261</pages><issn>0732-8893</issn><eissn>1879-0070</eissn><abstract>Implant sonication is considered a useful method for the diagnosis of implant-related infections. We designed an in vitro study using Ti6Al4V discs and 5 different bacteria to determine the optimal sonication time for recovery of most bacteria tested to enable use of sonication in clinical practice for microbiological diagnosis of implant-related infections. We carried out a specific protocol for the adherence and subsequent biofilm formation on the materials used. The discs were then sonicated and the retrieved bacteria were quantified. From minute 1 to 5, the amount of recovered organisms grew progressively for all bacteria. Between minute 6 and minute 10, the number was irregular for all strains except E. coli, though no pattern was evidenced. E. coli was the only microorganism with a progressive increase in liberation throughout the process. Significant differences were observed in each of the 10minutes analyzed as concerns the release of the 5 strains (P<0.021) as well as in the mean dislodgement (of the 10minutes) of all tested strains (P<0.00001).
Considering that infections in which biofilms are involved could be polymicrobial, we concluded that 5minutes is the optimal time of sonication in order to recover the maximum amount of most bacteria attached to Ti6Al4V discs.
•An in vitro trial using Ti discs and 5 different bacterial strains is proposed.•The study includes biofilm formation and exposure to ultrasound of samples.•The optimal sonication time to recover the maximum bacteria attached is 5minutes.•Gram-positive strains have higher release from the discs than gram negatives.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>29037465</pmid><doi>10.1016/j.diagmicrobio.2017.08.018</doi><tpages>4</tpages></addata></record> |
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| ispartof | Diagnostic microbiology and infectious disease, 2017-12, Vol.89 (4), p.258-261 |
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| subjects | Biofilm Biofilms - growth & development Bioimplants Colony Count, Microbial Enterococcus faecalis - growth & development Enterococcus faecalis - isolation & purification Escherichia coli - growth & development Escherichia coli - isolation & purification Prostheses and Implants Prosthesis-Related Infections Pseudomonas aeruginosa - growth & development Pseudomonas aeruginosa - isolation & purification Release of bacteria Sonication Staphylococcus aureus - growth & development Staphylococcus aureus - isolation & purification Staphylococcus epidermidis - growth & development Staphylococcus epidermidis - isolation & purification Ti6Al4V Time Time Factors Titanium - chemistry |
| title | Influence of exposure time on the release of bacteria from a biofilm on Ti6Al4V discs using sonication: An in vitro model |
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