Applying a riboregulator as a new chromosomal gene regulation tool for higher glycogen production in Synechocystis sp. PCC 6803
Cyanobacteria are one of the most attractive hosts for biofuel production; however, genetic approaches to regulate specific chromosomal genes in cyanobacteria remain limited. With the aim of developing a novel method to regulate chromosomal gene expression in cyanobacteria, we focused on riboregulat...
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| Veröffentlicht in: | Applied microbiology and biotechnology 2017-12, Vol.101 (23-24), p.8465-8474 |
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| creator | Ueno, Kinuko Sakai, Yuta Shono, Chika Sakamoto, Ippei Tsukakoshi, Kaori Hihara, Yukako Sode, Koji Ikebukuro, Kazunori |
| description | Cyanobacteria are one of the most attractive hosts for biofuel production; however, genetic approaches to regulate specific chromosomal genes in cyanobacteria remain limited. With the aim of developing a novel method to regulate chromosomal gene expression in cyanobacteria, we focused on riboregulatory technology. Riboregulators are composed of two RNA fragments whose interaction leads to target gene regulation with high specificity. In this study, we inserted a riboregulator sequence upstream of the chromosomal gene encoding AbrB-like transcriptional regulator, cyAbrB2, to investigate the utility of this tool. The inserted riboregulator was able to regulate
cyabrB2
gene expression, with a high ON-OFF ratio up to approximately 50-fold. The transcription levels of several genes for which cyAbrB2 acts as a transcriptional upregulator were also decreased
.
Further, the cyAbrB2 expression-repressed mutant showed high glycogen accumulation, equivalent to that in the
cyabrB2
deletion mutant (Δ
cyabrB2
). Phenotypic similarities between the
cyabrB2
expression-repressed mutant and the Δ
cyabrB2
mutant suggest that the riboregulator can potentially be used as a new chromosomal gene regulation tool in cyanobacteria. |
| doi_str_mv | 10.1007/s00253-017-8570-4 |
| format | Article |
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cyabrB2
gene expression, with a high ON-OFF ratio up to approximately 50-fold. The transcription levels of several genes for which cyAbrB2 acts as a transcriptional upregulator were also decreased
.
Further, the cyAbrB2 expression-repressed mutant showed high glycogen accumulation, equivalent to that in the
cyabrB2
deletion mutant (Δ
cyabrB2
). Phenotypic similarities between the
cyabrB2
expression-repressed mutant and the Δ
cyabrB2
mutant suggest that the riboregulator can potentially be used as a new chromosomal gene regulation tool in cyanobacteria.</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s00253-017-8570-4</identifier><identifier>PMID: 29038975</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Applied Genetics and Molecular Biotechnology ; Biofuels ; Biomedical and Life Sciences ; Biotechnology ; Cyanobacteria ; Deletion mutant ; Gene expression ; Gene Expression Regulation, Bacterial ; Gene regulation ; Genes ; Genes, Regulator ; Genetic aspects ; Genetic regulation ; Glycogen ; Glycogen - biosynthesis ; Life Sciences ; Metabolic Engineering - methods ; Methods ; Microbial Genetics and Genomics ; Microbiology ; Ribonucleic acid ; RNA ; Synechocystis - genetics ; Synechocystis - metabolism ; Transcription ; Transcription, Genetic</subject><ispartof>Applied microbiology and biotechnology, 2017-12, Vol.101 (23-24), p.8465-8474</ispartof><rights>Springer-Verlag GmbH Germany 2017</rights><rights>COPYRIGHT 2017 Springer</rights><rights>Applied Microbiology and Biotechnology is a copyright of Springer, (2017). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c576t-662da1e63c1a8ee2721e61bda25cbd930be2bd25ed3a9aaaf7f7d8481100204b3</citedby><cites>FETCH-LOGICAL-c576t-662da1e63c1a8ee2721e61bda25cbd930be2bd25ed3a9aaaf7f7d8481100204b3</cites><orcidid>0000-0003-2838-0562</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00253-017-8570-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00253-017-8570-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29038975$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ueno, Kinuko</creatorcontrib><creatorcontrib>Sakai, Yuta</creatorcontrib><creatorcontrib>Shono, Chika</creatorcontrib><creatorcontrib>Sakamoto, Ippei</creatorcontrib><creatorcontrib>Tsukakoshi, Kaori</creatorcontrib><creatorcontrib>Hihara, Yukako</creatorcontrib><creatorcontrib>Sode, Koji</creatorcontrib><creatorcontrib>Ikebukuro, Kazunori</creatorcontrib><title>Applying a riboregulator as a new chromosomal gene regulation tool for higher glycogen production in Synechocystis sp. PCC 6803</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><addtitle>Appl Microbiol Biotechnol</addtitle><description>Cyanobacteria are one of the most attractive hosts for biofuel production; however, genetic approaches to regulate specific chromosomal genes in cyanobacteria remain limited. With the aim of developing a novel method to regulate chromosomal gene expression in cyanobacteria, we focused on riboregulatory technology. Riboregulators are composed of two RNA fragments whose interaction leads to target gene regulation with high specificity. In this study, we inserted a riboregulator sequence upstream of the chromosomal gene encoding AbrB-like transcriptional regulator, cyAbrB2, to investigate the utility of this tool. The inserted riboregulator was able to regulate
cyabrB2
gene expression, with a high ON-OFF ratio up to approximately 50-fold. The transcription levels of several genes for which cyAbrB2 acts as a transcriptional upregulator were also decreased
.
Further, the cyAbrB2 expression-repressed mutant showed high glycogen accumulation, equivalent to that in the
cyabrB2
deletion mutant (Δ
cyabrB2
). Phenotypic similarities between the
cyabrB2
expression-repressed mutant and the Δ
cyabrB2
mutant suggest that the riboregulator can potentially be used as a new chromosomal gene regulation tool in cyanobacteria.</description><subject>Applied Genetics and Molecular Biotechnology</subject><subject>Biofuels</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cyanobacteria</subject><subject>Deletion mutant</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Gene regulation</subject><subject>Genes</subject><subject>Genes, Regulator</subject><subject>Genetic aspects</subject><subject>Genetic regulation</subject><subject>Glycogen</subject><subject>Glycogen - biosynthesis</subject><subject>Life Sciences</subject><subject>Metabolic Engineering - methods</subject><subject>Methods</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Synechocystis - genetics</subject><subject>Synechocystis - metabolism</subject><subject>Transcription</subject><subject>Transcription, Genetic</subject><issn>0175-7598</issn><issn>1432-0614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp10k2P0zAQBuAIgdiy8AO4IEtc4JBiO7GdHKuKj5VWArFwthxnknqV2MVOtOTEX2dKy0cRyIfI9jOjcfRm2VNG14xS9SpRykWRU6bySiial_eyFSsLnlPJyvvZCi9ErkRdXWSPUrqllPFKyofZBa9pUdVKrLJvm_1-WJzviSHRNSFCPw9mCpGYhEce7ojdxTCGFEYzkB48kJNxwZMphIF0qHeu30Ek_bDYgIjsY2hn-8M4T24WD3YX7JIml0jar8mH7ZbIihaPswedGRI8OX0vs89vXn_avsuv37-92m6ucyuUnHIpeWsYyMIyUwFwxXHDmtZwYZu2LmgDvGm5gLYwtTGmU51qq7Ji-J84LZviMntx7IuDfZkhTXp0ycIwGA9hTprVgqOlqkL6_C96G-bocTpUEsdBVf9WvRlAO9-FKRp7aKo3gpW1rIWgqNb_ULhaGJ0NHjqH52cFL88K0EzwderNnJK-uvl4btnR2hhSitDpfXSjiYtmVB8Coo8B0ZgDfQiILrHm2elxczNC-6viZyIQ8CNIeOV7iH-8_r9dvwNNIMP9</recordid><startdate>20171201</startdate><enddate>20171201</enddate><creator>Ueno, Kinuko</creator><creator>Sakai, Yuta</creator><creator>Shono, Chika</creator><creator>Sakamoto, Ippei</creator><creator>Tsukakoshi, Kaori</creator><creator>Hihara, Yukako</creator><creator>Sode, Koji</creator><creator>Ikebukuro, Kazunori</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>LK8</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2838-0562</orcidid></search><sort><creationdate>20171201</creationdate><title>Applying a riboregulator as a new chromosomal gene regulation tool for higher glycogen production in Synechocystis sp. PCC 6803</title><author>Ueno, Kinuko ; Sakai, Yuta ; Shono, Chika ; Sakamoto, Ippei ; Tsukakoshi, Kaori ; Hihara, Yukako ; Sode, Koji ; Ikebukuro, Kazunori</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c576t-662da1e63c1a8ee2721e61bda25cbd930be2bd25ed3a9aaaf7f7d8481100204b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Applied Genetics and Molecular Biotechnology</topic><topic>Biofuels</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Cyanobacteria</topic><topic>Deletion mutant</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Gene regulation</topic><topic>Genes</topic><topic>Genes, Regulator</topic><topic>Genetic aspects</topic><topic>Genetic regulation</topic><topic>Glycogen</topic><topic>Glycogen - biosynthesis</topic><topic>Life Sciences</topic><topic>Metabolic Engineering - methods</topic><topic>Methods</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Synechocystis - 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Academic</collection><jtitle>Applied microbiology and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ueno, Kinuko</au><au>Sakai, Yuta</au><au>Shono, Chika</au><au>Sakamoto, Ippei</au><au>Tsukakoshi, Kaori</au><au>Hihara, Yukako</au><au>Sode, Koji</au><au>Ikebukuro, Kazunori</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Applying a riboregulator as a new chromosomal gene regulation tool for higher glycogen production in Synechocystis sp. PCC 6803</atitle><jtitle>Applied microbiology and biotechnology</jtitle><stitle>Appl Microbiol Biotechnol</stitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>2017-12-01</date><risdate>2017</risdate><volume>101</volume><issue>23-24</issue><spage>8465</spage><epage>8474</epage><pages>8465-8474</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><abstract>Cyanobacteria are one of the most attractive hosts for biofuel production; however, genetic approaches to regulate specific chromosomal genes in cyanobacteria remain limited. With the aim of developing a novel method to regulate chromosomal gene expression in cyanobacteria, we focused on riboregulatory technology. Riboregulators are composed of two RNA fragments whose interaction leads to target gene regulation with high specificity. In this study, we inserted a riboregulator sequence upstream of the chromosomal gene encoding AbrB-like transcriptional regulator, cyAbrB2, to investigate the utility of this tool. The inserted riboregulator was able to regulate
cyabrB2
gene expression, with a high ON-OFF ratio up to approximately 50-fold. The transcription levels of several genes for which cyAbrB2 acts as a transcriptional upregulator were also decreased
.
Further, the cyAbrB2 expression-repressed mutant showed high glycogen accumulation, equivalent to that in the
cyabrB2
deletion mutant (Δ
cyabrB2
). Phenotypic similarities between the
cyabrB2
expression-repressed mutant and the Δ
cyabrB2
mutant suggest that the riboregulator can potentially be used as a new chromosomal gene regulation tool in cyanobacteria.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>29038975</pmid><doi>10.1007/s00253-017-8570-4</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-2838-0562</orcidid></addata></record> |
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| identifier | ISSN: 0175-7598 |
| ispartof | Applied microbiology and biotechnology, 2017-12, Vol.101 (23-24), p.8465-8474 |
| issn | 0175-7598 1432-0614 |
| language | eng |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Applied Genetics and Molecular Biotechnology Biofuels Biomedical and Life Sciences Biotechnology Cyanobacteria Deletion mutant Gene expression Gene Expression Regulation, Bacterial Gene regulation Genes Genes, Regulator Genetic aspects Genetic regulation Glycogen Glycogen - biosynthesis Life Sciences Metabolic Engineering - methods Methods Microbial Genetics and Genomics Microbiology Ribonucleic acid RNA Synechocystis - genetics Synechocystis - metabolism Transcription Transcription, Genetic |
| title | Applying a riboregulator as a new chromosomal gene regulation tool for higher glycogen production in Synechocystis sp. PCC 6803 |
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