Quantitative competitive (qc) RT-PCR as a tool in biomarker analysis
All inducible proteins which respond to known pollutants are potential molecular biomarkers. Quantitative competitive (qc) RT-PCR represents a uniquely sensitive tool for measuring the extent of induction of molecular biomarkers such as metallothionein, which is responsive to inducers that include a...
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| Veröffentlicht in: | Biomarkers 2001, Vol.6 (1), p.7-14 |
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| container_title | Biomarkers |
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| creator | Evans, Clive W. Wilson, David A. Mills, Geoffrey N. |
| description | All inducible proteins which respond to known pollutants are potential molecular biomarkers. Quantitative competitive (qc) RT-PCR represents a uniquely sensitive tool for measuring the extent of induction of molecular biomarkers such as metallothionein, which is responsive to inducers that include a range of heavy metals. Using the yellowbelly flounder Rhombosolea leporina collected from sites in the Manukau Harbour and Hauraki Gulf (Auckland, New Zealand) as an indicator species, we describe the methodology underpinning the use of qcRT-PCR as a tool in biomarker analysis with reference to the induction of metallothionein. The results show reasonable correlation between the extent of metallothionein induction and the liver burden of Cu and Zn. |
| doi_str_mv | 10.1080/135475001452733 |
| format | Article |
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| identifier | ISSN: 1354-750X |
| ispartof | Biomarkers, 2001, Vol.6 (1), p.7-14 |
| issn | 1354-750X 1366-5804 |
| language | eng |
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| source | Taylor & Francis Medical Library - CRKN; Taylor & Francis Journals Complete |
| subjects | Flounder Biomarker Metallothionein Rt-PCR Heavy Metals Pollution Marine Platichthys Pleuronectiformes Rhombosolea leporina |
| title | Quantitative competitive (qc) RT-PCR as a tool in biomarker analysis |
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