PTGER2 activation induces PTGS-2 and growth factor gene expression in endometrial epithelial cells of cattle
The prostaglandin E2 receptor 2 (PTGER2) is present in the endometrium and its gene expression is accompanied with endometrial growth, however, it is unknown whether there is endometrial repair through stimulation of growth factor gene expression that is promoted by PTGER2 activation in cattle. The...
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| Veröffentlicht in: | Animal reproduction science 2017-12, Vol.187, p.54-63 |
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| creator | Gao, Long Liu, Bo Mao, Wei Gao, Ruifeng Zhang, Shuangyi Duritahala Fu, Changqi Shen, Yuan Zhang, Ying Zhang, Nan Wu, Jindi Deng, Yang Wu, Xing Cao, Jinshan |
| description | The prostaglandin E2 receptor 2 (PTGER2) is present in the endometrium and its gene expression is accompanied with endometrial growth, however, it is unknown whether there is endometrial repair through stimulation of growth factor gene expression that is promoted by PTGER2 activation in cattle. The aim of this study was to investigate whether PTGER2 activation can induce prostaglandin-endoperoxide synthase-2 (PTGS-2) and growth factor gene expression by activating PKA and ERK signaling pathways in endometrial epithelial cells of cattle. Results demonstrated that the PTGER2 agonist, butaprost, induced cAMP/PKA and ERK activation and up-regulated PTGS-2, VEGF, CTGF, TGF-β1 and IL-8 gene expression. These activations were less after PTGER2 antagonist, AH6809, treatment. Data suggested that PTGS-2 gene expression was induced by PTGER2 activation through the PKA and ERK pathways. Furthermore, PTGER2 activation promoted several growth factor gene expressions in endometrial epithelial cells. One potential implication of this finding is that PTGER2 activation in the endometrium of cattle could induce endometrial repair by stimulating VEGF, CTGF, TGF-β1 and IL-8 gene expression. |
| doi_str_mv | 10.1016/j.anireprosci.2017.10.005 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1951569345</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0378432017304220</els_id><sourcerecordid>1951569345</sourcerecordid><originalsourceid>FETCH-LOGICAL-c443t-e2a3198d2df110470381dc24ed711c7ba98b07947eae98ea719d34994b1375473</originalsourceid><addsrcrecordid>eNqNkE1vGyEQhlHVqnbc_oWI3HpZlwHWLMfKctJKkRo1yRlhmI2x1rsOYCf592HltMqxJxDvxwwPIRfA5sBg8X07t32IuI9DcmHOGajyPmes_kCm0ChRcS74RzJlQjWVFJxNyFlKW8aYWiz0ZzLhmgkBoKaku7m7Wv3h1LocjjaHoaeh9weHiRbltipK7-lDHJ7yhrbFNUT6gD1SfN5HTOkUoNj7YYc5BttR3Ie8wW68Ouy6RIeWOptzh1_Ip9Z2Cb--nTNyf7m6W_6srn9f_Vr-uK6clCJXyK0A3XjuWwAmFRMNeMclegXg1NrqZs2Ulgot6gatAu2F1FquQahaKjEj3069hdDjAVM2u5DGXWyPwyEZ0DXUCy1kXaz6ZHUFZorYmn0MOxtfDDAzwjZb8w62GWGPUoFdsudvYw7rHfp_yb90i2F5MmD57DFgNKUCe4e-FLps_BD-Y8wrCfKWVg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1951569345</pqid></control><display><type>article</type><title>PTGER2 activation induces PTGS-2 and growth factor gene expression in endometrial epithelial cells of cattle</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Gao, Long ; Liu, Bo ; Mao, Wei ; Gao, Ruifeng ; Zhang, Shuangyi ; Duritahala ; Fu, Changqi ; Shen, Yuan ; Zhang, Ying ; Zhang, Nan ; Wu, Jindi ; Deng, Yang ; Wu, Xing ; Cao, Jinshan</creator><creatorcontrib>Gao, Long ; Liu, Bo ; Mao, Wei ; Gao, Ruifeng ; Zhang, Shuangyi ; Duritahala ; Fu, Changqi ; Shen, Yuan ; Zhang, Ying ; Zhang, Nan ; Wu, Jindi ; Deng, Yang ; Wu, Xing ; Cao, Jinshan</creatorcontrib><description>The prostaglandin E2 receptor 2 (PTGER2) is present in the endometrium and its gene expression is accompanied with endometrial growth, however, it is unknown whether there is endometrial repair through stimulation of growth factor gene expression that is promoted by PTGER2 activation in cattle. The aim of this study was to investigate whether PTGER2 activation can induce prostaglandin-endoperoxide synthase-2 (PTGS-2) and growth factor gene expression by activating PKA and ERK signaling pathways in endometrial epithelial cells of cattle. Results demonstrated that the PTGER2 agonist, butaprost, induced cAMP/PKA and ERK activation and up-regulated PTGS-2, VEGF, CTGF, TGF-β1 and IL-8 gene expression. These activations were less after PTGER2 antagonist, AH6809, treatment. Data suggested that PTGS-2 gene expression was induced by PTGER2 activation through the PKA and ERK pathways. Furthermore, PTGER2 activation promoted several growth factor gene expressions in endometrial epithelial cells. One potential implication of this finding is that PTGER2 activation in the endometrium of cattle could induce endometrial repair by stimulating VEGF, CTGF, TGF-β1 and IL-8 gene expression.</description><identifier>ISSN: 0378-4320</identifier><identifier>EISSN: 1873-2232</identifier><identifier>DOI: 10.1016/j.anireprosci.2017.10.005</identifier><identifier>PMID: 29033117</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Alprostadil - analogs & derivatives ; Alprostadil - pharmacology ; Animals ; Bovine endometrial epithelial cell ; Cattle - genetics ; Cattle - metabolism ; Cells, Cultured ; Cyclooxygenase 2 - genetics ; Cyclooxygenase 2 - metabolism ; Dinoprostone - genetics ; Dinoprostone - metabolism ; Endometrium - cytology ; Endometrium - drug effects ; Endometrium - metabolism ; Epithelial Cells - cytology ; Epithelial Cells - drug effects ; Epithelial Cells - metabolism ; Female ; Gene Expression Regulation - drug effects ; Growth factors ; Intercellular Signaling Peptides and Proteins - genetics ; Interleukin-8 - genetics ; Interleukin-8 - metabolism ; MAP Kinase Signaling System - drug effects ; Prostaglandin Antagonists - pharmacology ; PTGER2 activation ; PTGS-2 ; Receptors, Prostaglandin E, EP2 Subtype - chemistry ; Receptors, Prostaglandin E, EP2 Subtype - genetics ; Receptors, Prostaglandin E, EP2 Subtype - metabolism ; Transforming Growth Factor beta1 - genetics ; Transforming Growth Factor beta1 - metabolism ; Vascular Endothelial Growth Factor A - genetics ; Vascular Endothelial Growth Factor A - metabolism ; Xanthones - pharmacology</subject><ispartof>Animal reproduction science, 2017-12, Vol.187, p.54-63</ispartof><rights>2017 Elsevier B.V.</rights><rights>Copyright © 2017 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c443t-e2a3198d2df110470381dc24ed711c7ba98b07947eae98ea719d34994b1375473</citedby><cites>FETCH-LOGICAL-c443t-e2a3198d2df110470381dc24ed711c7ba98b07947eae98ea719d34994b1375473</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378432017304220$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29033117$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gao, Long</creatorcontrib><creatorcontrib>Liu, Bo</creatorcontrib><creatorcontrib>Mao, Wei</creatorcontrib><creatorcontrib>Gao, Ruifeng</creatorcontrib><creatorcontrib>Zhang, Shuangyi</creatorcontrib><creatorcontrib>Duritahala</creatorcontrib><creatorcontrib>Fu, Changqi</creatorcontrib><creatorcontrib>Shen, Yuan</creatorcontrib><creatorcontrib>Zhang, Ying</creatorcontrib><creatorcontrib>Zhang, Nan</creatorcontrib><creatorcontrib>Wu, Jindi</creatorcontrib><creatorcontrib>Deng, Yang</creatorcontrib><creatorcontrib>Wu, Xing</creatorcontrib><creatorcontrib>Cao, Jinshan</creatorcontrib><title>PTGER2 activation induces PTGS-2 and growth factor gene expression in endometrial epithelial cells of cattle</title><title>Animal reproduction science</title><addtitle>Anim Reprod Sci</addtitle><description>The prostaglandin E2 receptor 2 (PTGER2) is present in the endometrium and its gene expression is accompanied with endometrial growth, however, it is unknown whether there is endometrial repair through stimulation of growth factor gene expression that is promoted by PTGER2 activation in cattle. The aim of this study was to investigate whether PTGER2 activation can induce prostaglandin-endoperoxide synthase-2 (PTGS-2) and growth factor gene expression by activating PKA and ERK signaling pathways in endometrial epithelial cells of cattle. Results demonstrated that the PTGER2 agonist, butaprost, induced cAMP/PKA and ERK activation and up-regulated PTGS-2, VEGF, CTGF, TGF-β1 and IL-8 gene expression. These activations were less after PTGER2 antagonist, AH6809, treatment. Data suggested that PTGS-2 gene expression was induced by PTGER2 activation through the PKA and ERK pathways. Furthermore, PTGER2 activation promoted several growth factor gene expressions in endometrial epithelial cells. One potential implication of this finding is that PTGER2 activation in the endometrium of cattle could induce endometrial repair by stimulating VEGF, CTGF, TGF-β1 and IL-8 gene expression.</description><subject>Alprostadil - analogs & derivatives</subject><subject>Alprostadil - pharmacology</subject><subject>Animals</subject><subject>Bovine endometrial epithelial cell</subject><subject>Cattle - genetics</subject><subject>Cattle - metabolism</subject><subject>Cells, Cultured</subject><subject>Cyclooxygenase 2 - genetics</subject><subject>Cyclooxygenase 2 - metabolism</subject><subject>Dinoprostone - genetics</subject><subject>Dinoprostone - metabolism</subject><subject>Endometrium - cytology</subject><subject>Endometrium - drug effects</subject><subject>Endometrium - metabolism</subject><subject>Epithelial Cells - cytology</subject><subject>Epithelial Cells - drug effects</subject><subject>Epithelial Cells - metabolism</subject><subject>Female</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Growth factors</subject><subject>Intercellular Signaling Peptides and Proteins - genetics</subject><subject>Interleukin-8 - genetics</subject><subject>Interleukin-8 - metabolism</subject><subject>MAP Kinase Signaling System - drug effects</subject><subject>Prostaglandin Antagonists - pharmacology</subject><subject>PTGER2 activation</subject><subject>PTGS-2</subject><subject>Receptors, Prostaglandin E, EP2 Subtype - chemistry</subject><subject>Receptors, Prostaglandin E, EP2 Subtype - genetics</subject><subject>Receptors, Prostaglandin E, EP2 Subtype - metabolism</subject><subject>Transforming Growth Factor beta1 - genetics</subject><subject>Transforming Growth Factor beta1 - metabolism</subject><subject>Vascular Endothelial Growth Factor A - genetics</subject><subject>Vascular Endothelial Growth Factor A - metabolism</subject><subject>Xanthones - pharmacology</subject><issn>0378-4320</issn><issn>1873-2232</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1vGyEQhlHVqnbc_oWI3HpZlwHWLMfKctJKkRo1yRlhmI2x1rsOYCf592HltMqxJxDvxwwPIRfA5sBg8X07t32IuI9DcmHOGajyPmes_kCm0ChRcS74RzJlQjWVFJxNyFlKW8aYWiz0ZzLhmgkBoKaku7m7Wv3h1LocjjaHoaeh9weHiRbltipK7-lDHJ7yhrbFNUT6gD1SfN5HTOkUoNj7YYc5BttR3Ie8wW68Ouy6RIeWOptzh1_Ip9Z2Cb--nTNyf7m6W_6srn9f_Vr-uK6clCJXyK0A3XjuWwAmFRMNeMclegXg1NrqZs2Ulgot6gatAu2F1FquQahaKjEj3069hdDjAVM2u5DGXWyPwyEZ0DXUCy1kXaz6ZHUFZorYmn0MOxtfDDAzwjZb8w62GWGPUoFdsudvYw7rHfp_yb90i2F5MmD57DFgNKUCe4e-FLps_BD-Y8wrCfKWVg</recordid><startdate>201712</startdate><enddate>201712</enddate><creator>Gao, Long</creator><creator>Liu, Bo</creator><creator>Mao, Wei</creator><creator>Gao, Ruifeng</creator><creator>Zhang, Shuangyi</creator><creator>Duritahala</creator><creator>Fu, Changqi</creator><creator>Shen, Yuan</creator><creator>Zhang, Ying</creator><creator>Zhang, Nan</creator><creator>Wu, Jindi</creator><creator>Deng, Yang</creator><creator>Wu, Xing</creator><creator>Cao, Jinshan</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201712</creationdate><title>PTGER2 activation induces PTGS-2 and growth factor gene expression in endometrial epithelial cells of cattle</title><author>Gao, Long ; Liu, Bo ; Mao, Wei ; Gao, Ruifeng ; Zhang, Shuangyi ; Duritahala ; Fu, Changqi ; Shen, Yuan ; Zhang, Ying ; Zhang, Nan ; Wu, Jindi ; Deng, Yang ; Wu, Xing ; Cao, Jinshan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c443t-e2a3198d2df110470381dc24ed711c7ba98b07947eae98ea719d34994b1375473</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Alprostadil - analogs & derivatives</topic><topic>Alprostadil - pharmacology</topic><topic>Animals</topic><topic>Bovine endometrial epithelial cell</topic><topic>Cattle - genetics</topic><topic>Cattle - metabolism</topic><topic>Cells, Cultured</topic><topic>Cyclooxygenase 2 - genetics</topic><topic>Cyclooxygenase 2 - metabolism</topic><topic>Dinoprostone - genetics</topic><topic>Dinoprostone - metabolism</topic><topic>Endometrium - cytology</topic><topic>Endometrium - drug effects</topic><topic>Endometrium - metabolism</topic><topic>Epithelial Cells - cytology</topic><topic>Epithelial Cells - drug effects</topic><topic>Epithelial Cells - metabolism</topic><topic>Female</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Growth factors</topic><topic>Intercellular Signaling Peptides and Proteins - genetics</topic><topic>Interleukin-8 - genetics</topic><topic>Interleukin-8 - metabolism</topic><topic>MAP Kinase Signaling System - drug effects</topic><topic>Prostaglandin Antagonists - pharmacology</topic><topic>PTGER2 activation</topic><topic>PTGS-2</topic><topic>Receptors, Prostaglandin E, EP2 Subtype - chemistry</topic><topic>Receptors, Prostaglandin E, EP2 Subtype - genetics</topic><topic>Receptors, Prostaglandin E, EP2 Subtype - metabolism</topic><topic>Transforming Growth Factor beta1 - genetics</topic><topic>Transforming Growth Factor beta1 - metabolism</topic><topic>Vascular Endothelial Growth Factor A - genetics</topic><topic>Vascular Endothelial Growth Factor A - metabolism</topic><topic>Xanthones - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gao, Long</creatorcontrib><creatorcontrib>Liu, Bo</creatorcontrib><creatorcontrib>Mao, Wei</creatorcontrib><creatorcontrib>Gao, Ruifeng</creatorcontrib><creatorcontrib>Zhang, Shuangyi</creatorcontrib><creatorcontrib>Duritahala</creatorcontrib><creatorcontrib>Fu, Changqi</creatorcontrib><creatorcontrib>Shen, Yuan</creatorcontrib><creatorcontrib>Zhang, Ying</creatorcontrib><creatorcontrib>Zhang, Nan</creatorcontrib><creatorcontrib>Wu, Jindi</creatorcontrib><creatorcontrib>Deng, Yang</creatorcontrib><creatorcontrib>Wu, Xing</creatorcontrib><creatorcontrib>Cao, Jinshan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Animal reproduction science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gao, Long</au><au>Liu, Bo</au><au>Mao, Wei</au><au>Gao, Ruifeng</au><au>Zhang, Shuangyi</au><au>Duritahala</au><au>Fu, Changqi</au><au>Shen, Yuan</au><au>Zhang, Ying</au><au>Zhang, Nan</au><au>Wu, Jindi</au><au>Deng, Yang</au><au>Wu, Xing</au><au>Cao, Jinshan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PTGER2 activation induces PTGS-2 and growth factor gene expression in endometrial epithelial cells of cattle</atitle><jtitle>Animal reproduction science</jtitle><addtitle>Anim Reprod Sci</addtitle><date>2017-12</date><risdate>2017</risdate><volume>187</volume><spage>54</spage><epage>63</epage><pages>54-63</pages><issn>0378-4320</issn><eissn>1873-2232</eissn><abstract>The prostaglandin E2 receptor 2 (PTGER2) is present in the endometrium and its gene expression is accompanied with endometrial growth, however, it is unknown whether there is endometrial repair through stimulation of growth factor gene expression that is promoted by PTGER2 activation in cattle. The aim of this study was to investigate whether PTGER2 activation can induce prostaglandin-endoperoxide synthase-2 (PTGS-2) and growth factor gene expression by activating PKA and ERK signaling pathways in endometrial epithelial cells of cattle. Results demonstrated that the PTGER2 agonist, butaprost, induced cAMP/PKA and ERK activation and up-regulated PTGS-2, VEGF, CTGF, TGF-β1 and IL-8 gene expression. These activations were less after PTGER2 antagonist, AH6809, treatment. Data suggested that PTGS-2 gene expression was induced by PTGER2 activation through the PKA and ERK pathways. Furthermore, PTGER2 activation promoted several growth factor gene expressions in endometrial epithelial cells. One potential implication of this finding is that PTGER2 activation in the endometrium of cattle could induce endometrial repair by stimulating VEGF, CTGF, TGF-β1 and IL-8 gene expression.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>29033117</pmid><doi>10.1016/j.anireprosci.2017.10.005</doi><tpages>10</tpages></addata></record> |
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| subjects | Alprostadil - analogs & derivatives Alprostadil - pharmacology Animals Bovine endometrial epithelial cell Cattle - genetics Cattle - metabolism Cells, Cultured Cyclooxygenase 2 - genetics Cyclooxygenase 2 - metabolism Dinoprostone - genetics Dinoprostone - metabolism Endometrium - cytology Endometrium - drug effects Endometrium - metabolism Epithelial Cells - cytology Epithelial Cells - drug effects Epithelial Cells - metabolism Female Gene Expression Regulation - drug effects Growth factors Intercellular Signaling Peptides and Proteins - genetics Interleukin-8 - genetics Interleukin-8 - metabolism MAP Kinase Signaling System - drug effects Prostaglandin Antagonists - pharmacology PTGER2 activation PTGS-2 Receptors, Prostaglandin E, EP2 Subtype - chemistry Receptors, Prostaglandin E, EP2 Subtype - genetics Receptors, Prostaglandin E, EP2 Subtype - metabolism Transforming Growth Factor beta1 - genetics Transforming Growth Factor beta1 - metabolism Vascular Endothelial Growth Factor A - genetics Vascular Endothelial Growth Factor A - metabolism Xanthones - pharmacology |
| title | PTGER2 activation induces PTGS-2 and growth factor gene expression in endometrial epithelial cells of cattle |
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