Heterologous expression of wheat TaRUB1 gene enhances disease resistance in Arabidopsis thaliana
Key message Expression of TaRUB1 gene in Arabidopsis thaliana elevates the level of disease-related genes in response to pathogen invasion through the accumulation of callose, necrotic cells, and the outbreak of ROS . Ubiquitin (Ub) and ubiquitin-like proteins are highly conserved in sequence and ca...
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| Veröffentlicht in: | Plant cell reports 2017-12, Vol.36 (12), p.1985-1994 |
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| container_end_page | 1994 |
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| container_issue | 12 |
| container_start_page | 1985 |
| container_title | Plant cell reports |
| container_volume | 36 |
| creator | Yang, Yanlin Wang, Wenqiang Xu, Tian Liu, Na Wang, Honggang Feng, Deshun |
| description | Key message
Expression of
TaRUB1
gene in
Arabidopsis thaliana
elevates the level of disease-related genes in response to pathogen invasion through the accumulation of callose, necrotic cells, and the outbreak of ROS
.
Ubiquitin (Ub) and ubiquitin-like proteins are highly conserved in sequence and can covalently bind and modify many intracellular proteins which can be recognized and degraded by 26S proteasome. Post-translational modification of proteins has become a hot research spot today. In the previous study, a cDNA of related-to-ubiquitin protein belonged to ubiquitin-like proteins, whose spatial structure comprised Ub and NEDD8, was obtained from wheat SN6306 by suppression-subtractive hybridization and was named
TaRUB1. TaRUB1
is induced by wheat powdery mildew and significantly upregulated in resistant wheat SN6306. In this study, heterologous expression of
TaRUB1
in
A. thaliana
was used to study the function of this gene in response to pathogen
Pseudomonas syringae
pv. Tomato DC3000 (
Pst
DC3000). Transgenic
A. thaliana
showed relatively fewer disease symptoms, accompanied by common inhibition of living body parasitic defense responses, accumulation of more callose and reactive oxygen species (ROS), and concentrated cell death, simultaneously antioxidant enzyme activities of superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase were higher than those in wild-type (WT) plant after infection with
Pst
DC3000. Meanwhile, hypersensitive cell death, which was possibly ROS burst, was also observed in transgenic
A. thaliana
. By quantitative reverse transcription-polymerase chain reaction analysis, some marker genes for hypersensitive response showed significantly higher transcriptional expression level in transgenic
A. thaliana
, which activates system-acquired resistance, than that of WT plants. Heterologous expression of
TaRUB1
can significantly enhance resistance to
Pst
DC3000 in
A. thaliana
, suggesting that
TaRUB1
is related to plant disease resistance. |
| doi_str_mv | 10.1007/s00299-017-2221-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1951561535</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1951561535</sourcerecordid><originalsourceid>FETCH-LOGICAL-c372t-ce22197a2a418acc9a39205fcadd5565bb5c63bc5257221388e99dac0d59c6753</originalsourceid><addsrcrecordid>eNp1kE9rGzEQxUVpSJw_H6CXIuill000krWyjmlom0AgUGzoTZnVju0Na8mV1iT59tHitJRATgNvfvNm5jH2CcQ5CGEushDS2kqAqaSUUMEHNoGpkpUU6vdHNhGmiMbA9Igd5_wgRGma-pAdSSuUnEo9YffXNFCKfVzFXeb0tE2UcxcDj0v-uCYc-Bx_Lb4BX1EgTmGNwVPmbZcJM_FCd3kYNd4Ffpmw6dq4LRof1th3GPCUHSyxz3T2Wk_Y4sf3-dV1dXv38-bq8rbyysih8lQesAYlTmGG3ltUVgq99Ni2Wte6abSvVeO11KaQajYja1v0otXW10arE_Z177tN8c-O8uA2XfbU9xiovObAatA1aDWiX96gD3GXQrlupCxoKP6Fgj3lU8w50dJtU7fB9OxAuDF-t4_flfjdGL-DMvP51XnXbKj9N_E37wLIPZBLK6wo_bf6XdcXLpaPgw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1959151138</pqid></control><display><type>article</type><title>Heterologous expression of wheat TaRUB1 gene enhances disease resistance in Arabidopsis thaliana</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Yang, Yanlin ; Wang, Wenqiang ; Xu, Tian ; Liu, Na ; Wang, Honggang ; Feng, Deshun</creator><creatorcontrib>Yang, Yanlin ; Wang, Wenqiang ; Xu, Tian ; Liu, Na ; Wang, Honggang ; Feng, Deshun</creatorcontrib><description>Key message
Expression of
TaRUB1
gene in
Arabidopsis thaliana
elevates the level of disease-related genes in response to pathogen invasion through the accumulation of callose, necrotic cells, and the outbreak of ROS
.
Ubiquitin (Ub) and ubiquitin-like proteins are highly conserved in sequence and can covalently bind and modify many intracellular proteins which can be recognized and degraded by 26S proteasome. Post-translational modification of proteins has become a hot research spot today. In the previous study, a cDNA of related-to-ubiquitin protein belonged to ubiquitin-like proteins, whose spatial structure comprised Ub and NEDD8, was obtained from wheat SN6306 by suppression-subtractive hybridization and was named
TaRUB1. TaRUB1
is induced by wheat powdery mildew and significantly upregulated in resistant wheat SN6306. In this study, heterologous expression of
TaRUB1
in
A. thaliana
was used to study the function of this gene in response to pathogen
Pseudomonas syringae
pv. Tomato DC3000 (
Pst
DC3000). Transgenic
A. thaliana
showed relatively fewer disease symptoms, accompanied by common inhibition of living body parasitic defense responses, accumulation of more callose and reactive oxygen species (ROS), and concentrated cell death, simultaneously antioxidant enzyme activities of superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase were higher than those in wild-type (WT) plant after infection with
Pst
DC3000. Meanwhile, hypersensitive cell death, which was possibly ROS burst, was also observed in transgenic
A. thaliana
. By quantitative reverse transcription-polymerase chain reaction analysis, some marker genes for hypersensitive response showed significantly higher transcriptional expression level in transgenic
A. thaliana
, which activates system-acquired resistance, than that of WT plants. Heterologous expression of
TaRUB1
can significantly enhance resistance to
Pst
DC3000 in
A. thaliana
, suggesting that
TaRUB1
is related to plant disease resistance.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/s00299-017-2221-1</identifier><identifier>PMID: 29032425</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Accumulation ; Airborne microorganisms ; Antioxidants ; Apoptosis ; Arabidopsis - genetics ; Arabidopsis - metabolism ; Arabidopsis - microbiology ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Arabidopsis thaliana ; Ascorbic acid ; Biomedical and Life Sciences ; Biotechnology ; Catalase ; Cell Biology ; Cell death ; Complementary DNA ; Conserved sequence ; Disease resistance ; Disease Resistance - genetics ; Disease Resistance - physiology ; Enzymatic activity ; Gene expression ; Gene Expression Regulation, Plant ; Genes ; Hybridization ; Hypersensitive response ; L-Ascorbate peroxidase ; Life Sciences ; Original Article ; Outbreaks ; Parasitic diseases ; Pathogens ; Peroxidase ; Plant Biochemistry ; Plant diseases ; Plant Diseases - genetics ; Plant Diseases - microbiology ; Plant immunity ; Plant Sciences ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - metabolism ; Plants, Genetically Modified - microbiology ; Polymerase chain reaction ; Post-translation ; Powdery mildew ; Proteasome 26S ; Protein Processing, Post-Translational ; Proteins ; Pseudomonas syringae - pathogenicity ; Reactive oxygen species ; Reactive Oxygen Species - metabolism ; Reverse transcription ; Superoxide dismutase ; Tomatoes ; Triticum ; Triticum - genetics ; Triticum - metabolism ; Triticum - microbiology ; Ubiquitin ; Ubiquitins - metabolism ; Wheat</subject><ispartof>Plant cell reports, 2017-12, Vol.36 (12), p.1985-1994</ispartof><rights>Springer-Verlag GmbH Germany 2017</rights><rights>Plant Cell Reports is a copyright of Springer, 2017.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-ce22197a2a418acc9a39205fcadd5565bb5c63bc5257221388e99dac0d59c6753</citedby><cites>FETCH-LOGICAL-c372t-ce22197a2a418acc9a39205fcadd5565bb5c63bc5257221388e99dac0d59c6753</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00299-017-2221-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00299-017-2221-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29032425$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Yanlin</creatorcontrib><creatorcontrib>Wang, Wenqiang</creatorcontrib><creatorcontrib>Xu, Tian</creatorcontrib><creatorcontrib>Liu, Na</creatorcontrib><creatorcontrib>Wang, Honggang</creatorcontrib><creatorcontrib>Feng, Deshun</creatorcontrib><title>Heterologous expression of wheat TaRUB1 gene enhances disease resistance in Arabidopsis thaliana</title><title>Plant cell reports</title><addtitle>Plant Cell Rep</addtitle><addtitle>Plant Cell Rep</addtitle><description>Key message
Expression of
TaRUB1
gene in
Arabidopsis thaliana
elevates the level of disease-related genes in response to pathogen invasion through the accumulation of callose, necrotic cells, and the outbreak of ROS
.
Ubiquitin (Ub) and ubiquitin-like proteins are highly conserved in sequence and can covalently bind and modify many intracellular proteins which can be recognized and degraded by 26S proteasome. Post-translational modification of proteins has become a hot research spot today. In the previous study, a cDNA of related-to-ubiquitin protein belonged to ubiquitin-like proteins, whose spatial structure comprised Ub and NEDD8, was obtained from wheat SN6306 by suppression-subtractive hybridization and was named
TaRUB1. TaRUB1
is induced by wheat powdery mildew and significantly upregulated in resistant wheat SN6306. In this study, heterologous expression of
TaRUB1
in
A. thaliana
was used to study the function of this gene in response to pathogen
Pseudomonas syringae
pv. Tomato DC3000 (
Pst
DC3000). Transgenic
A. thaliana
showed relatively fewer disease symptoms, accompanied by common inhibition of living body parasitic defense responses, accumulation of more callose and reactive oxygen species (ROS), and concentrated cell death, simultaneously antioxidant enzyme activities of superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase were higher than those in wild-type (WT) plant after infection with
Pst
DC3000. Meanwhile, hypersensitive cell death, which was possibly ROS burst, was also observed in transgenic
A. thaliana
. By quantitative reverse transcription-polymerase chain reaction analysis, some marker genes for hypersensitive response showed significantly higher transcriptional expression level in transgenic
A. thaliana
, which activates system-acquired resistance, than that of WT plants. Heterologous expression of
TaRUB1
can significantly enhance resistance to
Pst
DC3000 in
A. thaliana
, suggesting that
TaRUB1
is related to plant disease resistance.</description><subject>Accumulation</subject><subject>Airborne microorganisms</subject><subject>Antioxidants</subject><subject>Apoptosis</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis - microbiology</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Arabidopsis thaliana</subject><subject>Ascorbic acid</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Catalase</subject><subject>Cell Biology</subject><subject>Cell death</subject><subject>Complementary DNA</subject><subject>Conserved sequence</subject><subject>Disease resistance</subject><subject>Disease Resistance - genetics</subject><subject>Disease Resistance - physiology</subject><subject>Enzymatic activity</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes</subject><subject>Hybridization</subject><subject>Hypersensitive response</subject><subject>L-Ascorbate peroxidase</subject><subject>Life Sciences</subject><subject>Original Article</subject><subject>Outbreaks</subject><subject>Parasitic diseases</subject><subject>Pathogens</subject><subject>Peroxidase</subject><subject>Plant Biochemistry</subject><subject>Plant diseases</subject><subject>Plant Diseases - genetics</subject><subject>Plant Diseases - microbiology</subject><subject>Plant immunity</subject><subject>Plant Sciences</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Plants, Genetically Modified - metabolism</subject><subject>Plants, Genetically Modified - microbiology</subject><subject>Polymerase chain reaction</subject><subject>Post-translation</subject><subject>Powdery mildew</subject><subject>Proteasome 26S</subject><subject>Protein Processing, Post-Translational</subject><subject>Proteins</subject><subject>Pseudomonas syringae - pathogenicity</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Reverse transcription</subject><subject>Superoxide dismutase</subject><subject>Tomatoes</subject><subject>Triticum</subject><subject>Triticum - genetics</subject><subject>Triticum - metabolism</subject><subject>Triticum - microbiology</subject><subject>Ubiquitin</subject><subject>Ubiquitins - metabolism</subject><subject>Wheat</subject><issn>0721-7714</issn><issn>1432-203X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kE9rGzEQxUVpSJw_H6CXIuill000krWyjmlom0AgUGzoTZnVju0Na8mV1iT59tHitJRATgNvfvNm5jH2CcQ5CGEushDS2kqAqaSUUMEHNoGpkpUU6vdHNhGmiMbA9Igd5_wgRGma-pAdSSuUnEo9YffXNFCKfVzFXeb0tE2UcxcDj0v-uCYc-Bx_Lb4BX1EgTmGNwVPmbZcJM_FCd3kYNd4Ffpmw6dq4LRof1th3GPCUHSyxz3T2Wk_Y4sf3-dV1dXv38-bq8rbyysih8lQesAYlTmGG3ltUVgq99Ni2Wte6abSvVeO11KaQajYja1v0otXW10arE_Z177tN8c-O8uA2XfbU9xiovObAatA1aDWiX96gD3GXQrlupCxoKP6Fgj3lU8w50dJtU7fB9OxAuDF-t4_flfjdGL-DMvP51XnXbKj9N_E37wLIPZBLK6wo_bf6XdcXLpaPgw</recordid><startdate>20171201</startdate><enddate>20171201</enddate><creator>Yang, Yanlin</creator><creator>Wang, Wenqiang</creator><creator>Xu, Tian</creator><creator>Liu, Na</creator><creator>Wang, Honggang</creator><creator>Feng, Deshun</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20171201</creationdate><title>Heterologous expression of wheat TaRUB1 gene enhances disease resistance in Arabidopsis thaliana</title><author>Yang, Yanlin ; Wang, Wenqiang ; Xu, Tian ; Liu, Na ; Wang, Honggang ; Feng, Deshun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-ce22197a2a418acc9a39205fcadd5565bb5c63bc5257221388e99dac0d59c6753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Accumulation</topic><topic>Airborne microorganisms</topic><topic>Antioxidants</topic><topic>Apoptosis</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis - microbiology</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Arabidopsis thaliana</topic><topic>Ascorbic acid</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Catalase</topic><topic>Cell Biology</topic><topic>Cell death</topic><topic>Complementary DNA</topic><topic>Conserved sequence</topic><topic>Disease resistance</topic><topic>Disease Resistance - genetics</topic><topic>Disease Resistance - physiology</topic><topic>Enzymatic activity</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genes</topic><topic>Hybridization</topic><topic>Hypersensitive response</topic><topic>L-Ascorbate peroxidase</topic><topic>Life Sciences</topic><topic>Original Article</topic><topic>Outbreaks</topic><topic>Parasitic diseases</topic><topic>Pathogens</topic><topic>Peroxidase</topic><topic>Plant Biochemistry</topic><topic>Plant diseases</topic><topic>Plant Diseases - genetics</topic><topic>Plant Diseases - microbiology</topic><topic>Plant immunity</topic><topic>Plant Sciences</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Plants, Genetically Modified - metabolism</topic><topic>Plants, Genetically Modified - microbiology</topic><topic>Polymerase chain reaction</topic><topic>Post-translation</topic><topic>Powdery mildew</topic><topic>Proteasome 26S</topic><topic>Protein Processing, Post-Translational</topic><topic>Proteins</topic><topic>Pseudomonas syringae - pathogenicity</topic><topic>Reactive oxygen species</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Reverse transcription</topic><topic>Superoxide dismutase</topic><topic>Tomatoes</topic><topic>Triticum</topic><topic>Triticum - genetics</topic><topic>Triticum - metabolism</topic><topic>Triticum - microbiology</topic><topic>Ubiquitin</topic><topic>Ubiquitins - metabolism</topic><topic>Wheat</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Yanlin</creatorcontrib><creatorcontrib>Wang, Wenqiang</creatorcontrib><creatorcontrib>Xu, Tian</creatorcontrib><creatorcontrib>Liu, Na</creatorcontrib><creatorcontrib>Wang, Honggang</creatorcontrib><creatorcontrib>Feng, Deshun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant cell reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Yanlin</au><au>Wang, Wenqiang</au><au>Xu, Tian</au><au>Liu, Na</au><au>Wang, Honggang</au><au>Feng, Deshun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Heterologous expression of wheat TaRUB1 gene enhances disease resistance in Arabidopsis thaliana</atitle><jtitle>Plant cell reports</jtitle><stitle>Plant Cell Rep</stitle><addtitle>Plant Cell Rep</addtitle><date>2017-12-01</date><risdate>2017</risdate><volume>36</volume><issue>12</issue><spage>1985</spage><epage>1994</epage><pages>1985-1994</pages><issn>0721-7714</issn><eissn>1432-203X</eissn><abstract>Key message
Expression of
TaRUB1
gene in
Arabidopsis thaliana
elevates the level of disease-related genes in response to pathogen invasion through the accumulation of callose, necrotic cells, and the outbreak of ROS
.
Ubiquitin (Ub) and ubiquitin-like proteins are highly conserved in sequence and can covalently bind and modify many intracellular proteins which can be recognized and degraded by 26S proteasome. Post-translational modification of proteins has become a hot research spot today. In the previous study, a cDNA of related-to-ubiquitin protein belonged to ubiquitin-like proteins, whose spatial structure comprised Ub and NEDD8, was obtained from wheat SN6306 by suppression-subtractive hybridization and was named
TaRUB1. TaRUB1
is induced by wheat powdery mildew and significantly upregulated in resistant wheat SN6306. In this study, heterologous expression of
TaRUB1
in
A. thaliana
was used to study the function of this gene in response to pathogen
Pseudomonas syringae
pv. Tomato DC3000 (
Pst
DC3000). Transgenic
A. thaliana
showed relatively fewer disease symptoms, accompanied by common inhibition of living body parasitic defense responses, accumulation of more callose and reactive oxygen species (ROS), and concentrated cell death, simultaneously antioxidant enzyme activities of superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase were higher than those in wild-type (WT) plant after infection with
Pst
DC3000. Meanwhile, hypersensitive cell death, which was possibly ROS burst, was also observed in transgenic
A. thaliana
. By quantitative reverse transcription-polymerase chain reaction analysis, some marker genes for hypersensitive response showed significantly higher transcriptional expression level in transgenic
A. thaliana
, which activates system-acquired resistance, than that of WT plants. Heterologous expression of
TaRUB1
can significantly enhance resistance to
Pst
DC3000 in
A. thaliana
, suggesting that
TaRUB1
is related to plant disease resistance.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>29032425</pmid><doi>10.1007/s00299-017-2221-1</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0721-7714 |
| ispartof | Plant cell reports, 2017-12, Vol.36 (12), p.1985-1994 |
| issn | 0721-7714 1432-203X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1951561535 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Accumulation Airborne microorganisms Antioxidants Apoptosis Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis - microbiology Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Arabidopsis thaliana Ascorbic acid Biomedical and Life Sciences Biotechnology Catalase Cell Biology Cell death Complementary DNA Conserved sequence Disease resistance Disease Resistance - genetics Disease Resistance - physiology Enzymatic activity Gene expression Gene Expression Regulation, Plant Genes Hybridization Hypersensitive response L-Ascorbate peroxidase Life Sciences Original Article Outbreaks Parasitic diseases Pathogens Peroxidase Plant Biochemistry Plant diseases Plant Diseases - genetics Plant Diseases - microbiology Plant immunity Plant Sciences Plants, Genetically Modified - genetics Plants, Genetically Modified - metabolism Plants, Genetically Modified - microbiology Polymerase chain reaction Post-translation Powdery mildew Proteasome 26S Protein Processing, Post-Translational Proteins Pseudomonas syringae - pathogenicity Reactive oxygen species Reactive Oxygen Species - metabolism Reverse transcription Superoxide dismutase Tomatoes Triticum Triticum - genetics Triticum - metabolism Triticum - microbiology Ubiquitin Ubiquitins - metabolism Wheat |
| title | Heterologous expression of wheat TaRUB1 gene enhances disease resistance in Arabidopsis thaliana |
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