αCP1 Mediates Stabilization of hTERT mRNA by Autocrine Human Growth Hormone

αCP1 Mediates Stabilization of hTERT mRNA by Autocrine Human Growth Hormone

We herein demonstrate that autocrine human growth hormone production in human mammary carcinoma cells results in increased telomerase activity as a result of specific up-regulation of telomerase catalytic subunit (human telomerase reverse transcriptase (hTERT)) mRNA and protein. This increase in hTE...

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Veröffentlicht in:The Journal of biological chemistry 2007-01, Vol.282 (1), p.680-690
Hauptverfasser: Emerald, B. Starling, Chen, Yong, Zhu, Tao, Zhu, Zhe, Lee, Kok-Onn, Gluckman, Peter D., Lobie, Peter E.
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container_end_page 690
container_issue 1
container_start_page 680
container_title The Journal of biological chemistry
container_volume 282
creator Emerald, B. Starling
Chen, Yong
Zhu, Tao
Zhu, Zhe
Lee, Kok-Onn
Gluckman, Peter D.
Lobie, Peter E.
description We herein demonstrate that autocrine human growth hormone production in human mammary carcinoma cells results in increased telomerase activity as a result of specific up-regulation of telomerase catalytic subunit (human telomerase reverse transcriptase (hTERT)) mRNA and protein. This increase in hTERT gene expression is not due to increased transcriptional activation of the hTERT promoter but is the result of increased stability of hTERT mRNA exerted by CU-rich cis-regulatory sequences present in the 3′-untranslated region of TERT mRNA. Autocrine human growth hormone up-regulates two poly(C)-binding proteins, αCP1 and αCP2, which bind to these cis-regulatory elements and stabilize hTERT mRNA. We have therefore demonstrated that post-transcriptional modulation of the level of hTERT mRNA is one mechanism for regulation of cellular telomerase activity.
doi_str_mv 10.1074/jbc.M600224200
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title αCP1 Mediates Stabilization of hTERT mRNA by Autocrine Human Growth Hormone
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