Temperature-sensitive cloning vector for Corynebacterium glutamicum

We constructed a temperature-sensitive form of the Corynebacterium glutamicum ATCC13869 cryptic plasmid, pBL1. The C. glutamicum/ Escherichia coli shuttle vector pSFK6, which is composed of pBL1 and the E. coli cloning vector pK1, was mutagenized in vitro by treatment with hydroxylamine, and introdu...

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Veröffentlicht in:	Plasmid 2006-11, Vol.56 (3), p.179-186
Hauptverfasser:	Nakamura, Jun, Kanno, Sohei, Kimura, Eiichiro, Matsui, Kazuhiko, Nakamatsu, Tsuyoshi, Wachi, Masaaki
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Base Sequence Corynebacterium glutamicum Corynebacterium glutamicum - genetics DNA Primers DNA Replication - genetics Escherichia coli Gene Components Genetic Vectors - genetics Hydroxylamine Molecular Sequence Data Mutagenesis, Site-Directed pBL1 Sequence Analysis, DNA Temperature Temperature-sensitive plasmid
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