Pathological and PCR detection of mycobacteriosis in pond-cultured Chinese soft shell turtles, Trionyx sinensis
Mycobacteriosis due to infection of Mycobacterium marinum is a common disease in pond-cultured Chinese soft shell turtles, especially in those surviving beyond their first year. The infected turtles independently showed either heterophilic or histiocytic granulomas in various organs such as the sple...
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| Veröffentlicht in: | Aquaculture 2006-11, Vol.261 (1), p.10-16 |
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| creator | Hsieh, C.Y. Chang, T.C. Shen, Y.L. Chang, C.D. Tu, C. Tung, M.C. Chen, L.C. Tsai, S.S. |
| description | Mycobacteriosis due to infection of
Mycobacterium marinum is a common disease in pond-cultured Chinese soft shell turtles, especially in those surviving beyond their first year. The infected turtles independently showed either heterophilic or histiocytic granulomas in various organs such as the spleen, liver, lungs, intestine, kidneys, stomach and pancreas. The heterophilic granuloma contained many acid-fast unbranching bacilli intracellularly in macrophages and extracellularly in the necrotic center. The histiocytic granuloma had only a few bacteria, mainly in the cytoplasm of Langhan's giant cells. The organisms were rarely observed in the advanced lesions of both types. Based on PCR assays for partial
hsp65 gene of
Mycobacterium spp., all of our strains were identified as
M. marinum which can be divided into two groups. The strains of the first group induced heterophilic granulomas and had very high nucleotide sequence identities (99.8%–100%) to the reference strains of
M. marinum (
AF456471) and
M. pseudoshottsii (
AY550226). Those strains of the second group caused histiocytic granulomas and also showed very high identities (99.8%–100%) to the reference strains of
M. marinum ATCC 927 (
AF456470) and
M. shottsii (
AY550225). However, when we compared the partial sequence of the
hsp65 gene from group one and two strains the identities between the two groups range from 98.8% to 99.3%, therefore we can not assert that these two belong to the same species. |
| doi_str_mv | 10.1016/j.aquaculture.2006.07.005 |
| format | Article |
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Mycobacterium marinum is a common disease in pond-cultured Chinese soft shell turtles, especially in those surviving beyond their first year. The infected turtles independently showed either heterophilic or histiocytic granulomas in various organs such as the spleen, liver, lungs, intestine, kidneys, stomach and pancreas. The heterophilic granuloma contained many acid-fast unbranching bacilli intracellularly in macrophages and extracellularly in the necrotic center. The histiocytic granuloma had only a few bacteria, mainly in the cytoplasm of Langhan's giant cells. The organisms were rarely observed in the advanced lesions of both types. Based on PCR assays for partial
hsp65 gene of
Mycobacterium spp., all of our strains were identified as
M. marinum which can be divided into two groups. The strains of the first group induced heterophilic granulomas and had very high nucleotide sequence identities (99.8%–100%) to the reference strains of
M. marinum (
AF456471) and
M. pseudoshottsii (
AY550226). Those strains of the second group caused histiocytic granulomas and also showed very high identities (99.8%–100%) to the reference strains of
M. marinum ATCC 927 (
AF456470) and
M. shottsii (
AY550225). However, when we compared the partial sequence of the
hsp65 gene from group one and two strains the identities between the two groups range from 98.8% to 99.3%, therefore we can not assert that these two belong to the same species.</description><identifier>ISSN: 0044-8486</identifier><identifier>EISSN: 1873-5622</identifier><identifier>DOI: 10.1016/j.aquaculture.2006.07.005</identifier><identifier>CODEN: AQCLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animal and plant ecology ; Animal aquaculture ; Animal diseases ; animal pathogenic bacteria ; Animal productions ; Animal, plant and microbial ecology ; Bacteria ; bacterial proteins ; Biological and medical sciences ; Biological variation ; Chinese soft shell turtle ; disease detection ; Fresh water ecosystems ; Fundamental and applied biological sciences. Psychology ; General aspects ; Genetics ; Heat shock protein 65 gene ( hsp65) ; heat shock proteins ; histopathology ; Mycobacteriosis ; Mycobacterium marinum ; pathogen identification ; Pathology ; Reptiles & amphibians ; sequence analysis ; Synecology ; Trionyx ; Trionyx sinensis ; turtle culture ; turtles</subject><ispartof>Aquaculture, 2006-11, Vol.261 (1), p.10-16</ispartof><rights>2006 Elsevier B.V.</rights><rights>2007 INIST-CNRS</rights><rights>Copyright Elsevier Sequoia S.A. Nov 16, 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-b655a2230d5247379a97b12932d0465795dd2426189949e65edbf0fe5f7f92ef3</citedby><cites>FETCH-LOGICAL-c499t-b655a2230d5247379a97b12932d0465795dd2426189949e65edbf0fe5f7f92ef3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0044848606004984$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18282635$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Hsieh, C.Y.</creatorcontrib><creatorcontrib>Chang, T.C.</creatorcontrib><creatorcontrib>Shen, Y.L.</creatorcontrib><creatorcontrib>Chang, C.D.</creatorcontrib><creatorcontrib>Tu, C.</creatorcontrib><creatorcontrib>Tung, M.C.</creatorcontrib><creatorcontrib>Chen, L.C.</creatorcontrib><creatorcontrib>Tsai, S.S.</creatorcontrib><title>Pathological and PCR detection of mycobacteriosis in pond-cultured Chinese soft shell turtles, Trionyx sinensis</title><title>Aquaculture</title><description>Mycobacteriosis due to infection of
Mycobacterium marinum is a common disease in pond-cultured Chinese soft shell turtles, especially in those surviving beyond their first year. The infected turtles independently showed either heterophilic or histiocytic granulomas in various organs such as the spleen, liver, lungs, intestine, kidneys, stomach and pancreas. The heterophilic granuloma contained many acid-fast unbranching bacilli intracellularly in macrophages and extracellularly in the necrotic center. The histiocytic granuloma had only a few bacteria, mainly in the cytoplasm of Langhan's giant cells. The organisms were rarely observed in the advanced lesions of both types. Based on PCR assays for partial
hsp65 gene of
Mycobacterium spp., all of our strains were identified as
M. marinum which can be divided into two groups. The strains of the first group induced heterophilic granulomas and had very high nucleotide sequence identities (99.8%–100%) to the reference strains of
M. marinum (
AF456471) and
M. pseudoshottsii (
AY550226). Those strains of the second group caused histiocytic granulomas and also showed very high identities (99.8%–100%) to the reference strains of
M. marinum ATCC 927 (
AF456470) and
M. shottsii (
AY550225). However, when we compared the partial sequence of the
hsp65 gene from group one and two strains the identities between the two groups range from 98.8% to 99.3%, therefore we can not assert that these two belong to the same species.</description><subject>Animal and plant ecology</subject><subject>Animal aquaculture</subject><subject>Animal diseases</subject><subject>animal pathogenic bacteria</subject><subject>Animal productions</subject><subject>Animal, plant and microbial ecology</subject><subject>Bacteria</subject><subject>bacterial proteins</subject><subject>Biological and medical sciences</subject><subject>Biological variation</subject><subject>Chinese soft shell turtle</subject><subject>disease detection</subject><subject>Fresh water ecosystems</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>Genetics</subject><subject>Heat shock protein 65 gene ( hsp65)</subject><subject>heat shock proteins</subject><subject>histopathology</subject><subject>Mycobacteriosis</subject><subject>Mycobacterium marinum</subject><subject>pathogen identification</subject><subject>Pathology</subject><subject>Reptiles & amphibians</subject><subject>sequence analysis</subject><subject>Synecology</subject><subject>Trionyx</subject><subject>Trionyx sinensis</subject><subject>turtle culture</subject><subject>turtles</subject><issn>0044-8486</issn><issn>1873-5622</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNqNkcFu1DAQhiMEEkvhGTBIcCJh7MROfERRoUiVqKA9W1573PUqa2_tpGLfHi-7EogTJx_8_TO_5quqNxQaClR83Db6YdFmmeYlYcMARAN9A8CfVCs69G3NBWNPqxVA19VDN4jn1Yuct1BAwemqijd63sQp3nujJ6KDJTfjd2JxRjP7GEh0ZHcwca3NjMnH7DPxgexjsPV5qSXjxgfMSHJ0M8kbnCZSPuYJ8wdyW0Lh8JPkgoSSflk9c3rK-Or8XlR3ny9vx6v6-tuXr-On69p0Us71WnCuGWvBctb1bS-17NeUyZZZ6ATvJbeWdUzQQcpOouBo1w4cctc7ydC1F9X709x9ig8L5lntfDalmg4Yl6yo5DAwDgV8-w-4jUsKpZti0PXAenqE5AkyKeac0Kl98judDoqCOnpQW_WXB3X0oKBXxUPJvjsv0Lnc2CUdjM9_BgxsYKI9cq9PnNNR6ftUmLsfDGgLlDIQv1uMJwLL4R49JpWNx2DQ-lR0KRv9f_T5BXykriQ</recordid><startdate>20061116</startdate><enddate>20061116</enddate><creator>Hsieh, C.Y.</creator><creator>Chang, T.C.</creator><creator>Shen, Y.L.</creator><creator>Chang, C.D.</creator><creator>Tu, C.</creator><creator>Tung, M.C.</creator><creator>Chen, L.C.</creator><creator>Tsai, S.S.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><general>Elsevier Sequoia S.A</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QR</scope><scope>7ST</scope><scope>7TN</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>SOI</scope></search><sort><creationdate>20061116</creationdate><title>Pathological and PCR detection of mycobacteriosis in pond-cultured Chinese soft shell turtles, Trionyx sinensis</title><author>Hsieh, C.Y. ; Chang, T.C. ; Shen, Y.L. ; Chang, C.D. ; Tu, C. ; Tung, M.C. ; Chen, L.C. ; Tsai, S.S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-b655a2230d5247379a97b12932d0465795dd2426189949e65edbf0fe5f7f92ef3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animal and plant ecology</topic><topic>Animal aquaculture</topic><topic>Animal diseases</topic><topic>animal pathogenic bacteria</topic><topic>Animal productions</topic><topic>Animal, plant and microbial ecology</topic><topic>Bacteria</topic><topic>bacterial proteins</topic><topic>Biological and medical sciences</topic><topic>Biological variation</topic><topic>Chinese soft shell turtle</topic><topic>disease detection</topic><topic>Fresh water ecosystems</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>Genetics</topic><topic>Heat shock protein 65 gene ( hsp65)</topic><topic>heat shock proteins</topic><topic>histopathology</topic><topic>Mycobacteriosis</topic><topic>Mycobacterium marinum</topic><topic>pathogen identification</topic><topic>Pathology</topic><topic>Reptiles & amphibians</topic><topic>sequence analysis</topic><topic>Synecology</topic><topic>Trionyx</topic><topic>Trionyx sinensis</topic><topic>turtle culture</topic><topic>turtles</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hsieh, C.Y.</creatorcontrib><creatorcontrib>Chang, T.C.</creatorcontrib><creatorcontrib>Shen, Y.L.</creatorcontrib><creatorcontrib>Chang, C.D.</creatorcontrib><creatorcontrib>Tu, C.</creatorcontrib><creatorcontrib>Tung, M.C.</creatorcontrib><creatorcontrib>Chen, L.C.</creatorcontrib><creatorcontrib>Tsai, S.S.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Environment Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Aquaculture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hsieh, C.Y.</au><au>Chang, T.C.</au><au>Shen, Y.L.</au><au>Chang, C.D.</au><au>Tu, C.</au><au>Tung, M.C.</au><au>Chen, L.C.</au><au>Tsai, S.S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pathological and PCR detection of mycobacteriosis in pond-cultured Chinese soft shell turtles, Trionyx sinensis</atitle><jtitle>Aquaculture</jtitle><date>2006-11-16</date><risdate>2006</risdate><volume>261</volume><issue>1</issue><spage>10</spage><epage>16</epage><pages>10-16</pages><issn>0044-8486</issn><eissn>1873-5622</eissn><coden>AQCLAL</coden><abstract>Mycobacteriosis due to infection of
Mycobacterium marinum is a common disease in pond-cultured Chinese soft shell turtles, especially in those surviving beyond their first year. The infected turtles independently showed either heterophilic or histiocytic granulomas in various organs such as the spleen, liver, lungs, intestine, kidneys, stomach and pancreas. The heterophilic granuloma contained many acid-fast unbranching bacilli intracellularly in macrophages and extracellularly in the necrotic center. The histiocytic granuloma had only a few bacteria, mainly in the cytoplasm of Langhan's giant cells. The organisms were rarely observed in the advanced lesions of both types. Based on PCR assays for partial
hsp65 gene of
Mycobacterium spp., all of our strains were identified as
M. marinum which can be divided into two groups. The strains of the first group induced heterophilic granulomas and had very high nucleotide sequence identities (99.8%–100%) to the reference strains of
M. marinum (
AF456471) and
M. pseudoshottsii (
AY550226). Those strains of the second group caused histiocytic granulomas and also showed very high identities (99.8%–100%) to the reference strains of
M. marinum ATCC 927 (
AF456470) and
M. shottsii (
AY550225). However, when we compared the partial sequence of the
hsp65 gene from group one and two strains the identities between the two groups range from 98.8% to 99.3%, therefore we can not assert that these two belong to the same species.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><doi>10.1016/j.aquaculture.2006.07.005</doi><tpages>7</tpages></addata></record> |
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| subjects | Animal and plant ecology Animal aquaculture Animal diseases animal pathogenic bacteria Animal productions Animal, plant and microbial ecology Bacteria bacterial proteins Biological and medical sciences Biological variation Chinese soft shell turtle disease detection Fresh water ecosystems Fundamental and applied biological sciences. Psychology General aspects Genetics Heat shock protein 65 gene ( hsp65) heat shock proteins histopathology Mycobacteriosis Mycobacterium marinum pathogen identification Pathology Reptiles & amphibians sequence analysis Synecology Trionyx Trionyx sinensis turtle culture turtles |
| title | Pathological and PCR detection of mycobacteriosis in pond-cultured Chinese soft shell turtles, Trionyx sinensis |
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