Dynamics of Huanglongbing-associated Bacterium Candidatus Liberibacter asiaticus in Citrus aurantifolia Swingle (Mexican Lime)
The bacterial disease citrus huanglongbing (HLB), associated with "Candidatus Liberibacter asiaticus" (C.Las) has severely impacted the citrus industry, causing a significant reduction in production and fruit quality. In the present study, it was monitored the C.Las population dynamics in...
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Veröffentlicht in: | Pakistan journal of biological sciences 2017, Vol.20 (3), p.113-123 |
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creator | Abel Lopez-Buenfil, Jose Abrahan Ramirez-Pool, Jose Ruiz-Medrano, Roberto Del Carmen Montes-Horcasitas, Maria Chavarin-Palacio, Claudio Moya-Hinojosa, Jesus Javier Trujillo-Arriaga, Francisco Carmona, Rosalia Lira Xoconostle-Cazares, Beatriz |
description | The bacterial disease citrus huanglongbing (HLB), associated with "Candidatus Liberibacter asiaticus" (C.Las) has severely impacted the citrus industry, causing a significant reduction in production and fruit quality. In the present study, it was monitored the C.Las population dynamics in symptomatic, HLB-positive Mexican lime trees (Citrus aurantifolia Swingle) in a tropical, citrus-producing area of Mexico. The objective of this study was to identify the dynamics of the population of huanglongbing-associated bacterium Candidatus Liberibacter asiaticus and its insect vector in Citrus aurantifolia Swingle (Mexican lime).
Leaf samples were collected every 2 months over a period of 26 months for quantification of bacterial titers and young and mature leaves were collected in each season to determine preferential sites of bacterial accumulation. The proportion of living and dead bacterial cells could be determined through the use of quantitative real-time PCR in the presence of ethidium monoazide (EMA-qPCR).
It was observed a lower bacterial titer at high temperatures in the infected trees relative to titers in mild weather, despite a higher accumulation of the insect vector Diaphorina citri in these conditions. This study also revealed seasonal fluctuations in the titers of bacteria in mature leaves when compared to young leaves. No statistically significant correlation between any meteorological variable, C.Las concentration and D. citri population could be drawn.
Although, HLB management strategies have focused on vector control, host tree phenology may be important. The evaluation of citrus phenology, C.Las concentration, ACP population and environmental conditions provides insights into the cyclical, seasonal variations of both the HLB pathogen and its vector. These findings should help in the design of integrative HLB control strategies that take into account the accumulation of the pathogen and the presence of its vector. |
doi_str_mv | 10.3923/pjbs.2017.113.123 |
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Leaf samples were collected every 2 months over a period of 26 months for quantification of bacterial titers and young and mature leaves were collected in each season to determine preferential sites of bacterial accumulation. The proportion of living and dead bacterial cells could be determined through the use of quantitative real-time PCR in the presence of ethidium monoazide (EMA-qPCR).
It was observed a lower bacterial titer at high temperatures in the infected trees relative to titers in mild weather, despite a higher accumulation of the insect vector Diaphorina citri in these conditions. This study also revealed seasonal fluctuations in the titers of bacteria in mature leaves when compared to young leaves. No statistically significant correlation between any meteorological variable, C.Las concentration and D. citri population could be drawn.
Although, HLB management strategies have focused on vector control, host tree phenology may be important. The evaluation of citrus phenology, C.Las concentration, ACP population and environmental conditions provides insights into the cyclical, seasonal variations of both the HLB pathogen and its vector. These findings should help in the design of integrative HLB control strategies that take into account the accumulation of the pathogen and the presence of its vector.</description><identifier>ISSN: 1028-8880</identifier><identifier>DOI: 10.3923/pjbs.2017.113.123</identifier><identifier>PMID: 29023002</identifier><language>eng</language><publisher>Pakistan</publisher><subject>Animals ; Bacterial Load ; Citrus aurantiifolia - growth & development ; Citrus aurantiifolia - microbiology ; Crops, Agricultural - growth & development ; Crops, Agricultural - microbiology ; Disease Vectors ; Hemiptera - microbiology ; Host-Pathogen Interactions ; Mexico ; Microbial Viability ; Plant Diseases - microbiology ; Plant Leaves - growth & development ; Plant Leaves - microbiology ; Population Dynamics ; Rhizobiaceae - genetics ; Rhizobiaceae - growth & development ; Seasons ; Time Factors ; Trees - growth & development ; Trees - microbiology ; Weather</subject><ispartof>Pakistan journal of biological sciences, 2017, Vol.20 (3), p.113-123</ispartof><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2593-c5bf6e74aec0c079150a6396deb68598b1327c736cc03541f68f8cd21925fec93</citedby><cites>FETCH-LOGICAL-c2593-c5bf6e74aec0c079150a6396deb68598b1327c736cc03541f68f8cd21925fec93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,4110,27902,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29023002$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Abel Lopez-Buenfil, Jose</creatorcontrib><creatorcontrib>Abrahan Ramirez-Pool, Jose</creatorcontrib><creatorcontrib>Ruiz-Medrano, Roberto</creatorcontrib><creatorcontrib>Del Carmen Montes-Horcasitas, Maria</creatorcontrib><creatorcontrib>Chavarin-Palacio, Claudio</creatorcontrib><creatorcontrib>Moya-Hinojosa, Jesus</creatorcontrib><creatorcontrib>Javier Trujillo-Arriaga, Francisco</creatorcontrib><creatorcontrib>Carmona, Rosalia Lira</creatorcontrib><creatorcontrib>Xoconostle-Cazares, Beatriz</creatorcontrib><title>Dynamics of Huanglongbing-associated Bacterium Candidatus Liberibacter asiaticus in Citrus aurantifolia Swingle (Mexican Lime)</title><title>Pakistan journal of biological sciences</title><addtitle>Pak J Biol Sci</addtitle><description>The bacterial disease citrus huanglongbing (HLB), associated with "Candidatus Liberibacter asiaticus" (C.Las) has severely impacted the citrus industry, causing a significant reduction in production and fruit quality. In the present study, it was monitored the C.Las population dynamics in symptomatic, HLB-positive Mexican lime trees (Citrus aurantifolia Swingle) in a tropical, citrus-producing area of Mexico. The objective of this study was to identify the dynamics of the population of huanglongbing-associated bacterium Candidatus Liberibacter asiaticus and its insect vector in Citrus aurantifolia Swingle (Mexican lime).
Leaf samples were collected every 2 months over a period of 26 months for quantification of bacterial titers and young and mature leaves were collected in each season to determine preferential sites of bacterial accumulation. The proportion of living and dead bacterial cells could be determined through the use of quantitative real-time PCR in the presence of ethidium monoazide (EMA-qPCR).
It was observed a lower bacterial titer at high temperatures in the infected trees relative to titers in mild weather, despite a higher accumulation of the insect vector Diaphorina citri in these conditions. This study also revealed seasonal fluctuations in the titers of bacteria in mature leaves when compared to young leaves. No statistically significant correlation between any meteorological variable, C.Las concentration and D. citri population could be drawn.
Although, HLB management strategies have focused on vector control, host tree phenology may be important. The evaluation of citrus phenology, C.Las concentration, ACP population and environmental conditions provides insights into the cyclical, seasonal variations of both the HLB pathogen and its vector. These findings should help in the design of integrative HLB control strategies that take into account the accumulation of the pathogen and the presence of its vector.</description><subject>Animals</subject><subject>Bacterial Load</subject><subject>Citrus aurantiifolia - growth & development</subject><subject>Citrus aurantiifolia - microbiology</subject><subject>Crops, Agricultural - growth & development</subject><subject>Crops, Agricultural - microbiology</subject><subject>Disease Vectors</subject><subject>Hemiptera - microbiology</subject><subject>Host-Pathogen Interactions</subject><subject>Mexico</subject><subject>Microbial Viability</subject><subject>Plant Diseases - microbiology</subject><subject>Plant Leaves - growth & development</subject><subject>Plant Leaves - microbiology</subject><subject>Population Dynamics</subject><subject>Rhizobiaceae - genetics</subject><subject>Rhizobiaceae - growth & development</subject><subject>Seasons</subject><subject>Time Factors</subject><subject>Trees - growth & development</subject><subject>Trees - microbiology</subject><subject>Weather</subject><issn>1028-8880</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kLtOwzAUhj2AaCk8AAvyWIYEX3KxRwiXIhUxALN14jiVq8QpcSLowrPj0sJ0jv7b8CF0QUnMJePXm3XpY0ZoHlPKY8r4EZpSwkQkhCATdOr9mpAkZ7k4QRMmCeOEsCn6vts6aK32uKvxYgS3ajq3Kq1bReB9py0MpsK3oAfT27HFBbjKVjCMHi9tGbTy18LgQ9LqIFuHCzv04YOxBzfYumss4NfPsNkYPH82X1aDC_XWXJ2h4xoab84Pd4beH-7fikW0fHl8Km6WkWap5JFOyzozeQJGE01ySVMCGZdZZcpMpFKUlLNc5zzTmvA0oXUmaqErRiVLa6Mln6H5fnfTdx-j8YNqrdemacCZbvSKypQkVFKahyjdR3Xfed-bWm1620K_VZSoHWq1Q612qFVArQLq0Lk8zI9la6r_xh9n_gM1Kn5p</recordid><startdate>2017</startdate><enddate>2017</enddate><creator>Abel Lopez-Buenfil, Jose</creator><creator>Abrahan Ramirez-Pool, Jose</creator><creator>Ruiz-Medrano, Roberto</creator><creator>Del Carmen Montes-Horcasitas, Maria</creator><creator>Chavarin-Palacio, Claudio</creator><creator>Moya-Hinojosa, Jesus</creator><creator>Javier Trujillo-Arriaga, Francisco</creator><creator>Carmona, Rosalia Lira</creator><creator>Xoconostle-Cazares, Beatriz</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2017</creationdate><title>Dynamics of Huanglongbing-associated Bacterium Candidatus Liberibacter asiaticus in Citrus aurantifolia Swingle (Mexican Lime)</title><author>Abel Lopez-Buenfil, Jose ; Abrahan Ramirez-Pool, Jose ; Ruiz-Medrano, Roberto ; Del Carmen Montes-Horcasitas, Maria ; Chavarin-Palacio, Claudio ; Moya-Hinojosa, Jesus ; Javier Trujillo-Arriaga, Francisco ; Carmona, Rosalia Lira ; Xoconostle-Cazares, Beatriz</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2593-c5bf6e74aec0c079150a6396deb68598b1327c736cc03541f68f8cd21925fec93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Bacterial Load</topic><topic>Citrus aurantiifolia - growth & development</topic><topic>Citrus aurantiifolia - microbiology</topic><topic>Crops, Agricultural - growth & development</topic><topic>Crops, Agricultural - microbiology</topic><topic>Disease Vectors</topic><topic>Hemiptera - microbiology</topic><topic>Host-Pathogen Interactions</topic><topic>Mexico</topic><topic>Microbial Viability</topic><topic>Plant Diseases - microbiology</topic><topic>Plant Leaves - growth & development</topic><topic>Plant Leaves - microbiology</topic><topic>Population Dynamics</topic><topic>Rhizobiaceae - genetics</topic><topic>Rhizobiaceae - growth & development</topic><topic>Seasons</topic><topic>Time Factors</topic><topic>Trees - growth & development</topic><topic>Trees - microbiology</topic><topic>Weather</topic><toplevel>online_resources</toplevel><creatorcontrib>Abel Lopez-Buenfil, Jose</creatorcontrib><creatorcontrib>Abrahan Ramirez-Pool, Jose</creatorcontrib><creatorcontrib>Ruiz-Medrano, Roberto</creatorcontrib><creatorcontrib>Del Carmen Montes-Horcasitas, Maria</creatorcontrib><creatorcontrib>Chavarin-Palacio, Claudio</creatorcontrib><creatorcontrib>Moya-Hinojosa, Jesus</creatorcontrib><creatorcontrib>Javier Trujillo-Arriaga, Francisco</creatorcontrib><creatorcontrib>Carmona, Rosalia Lira</creatorcontrib><creatorcontrib>Xoconostle-Cazares, Beatriz</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Pakistan journal of biological sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abel Lopez-Buenfil, Jose</au><au>Abrahan Ramirez-Pool, Jose</au><au>Ruiz-Medrano, Roberto</au><au>Del Carmen Montes-Horcasitas, Maria</au><au>Chavarin-Palacio, Claudio</au><au>Moya-Hinojosa, Jesus</au><au>Javier Trujillo-Arriaga, Francisco</au><au>Carmona, Rosalia Lira</au><au>Xoconostle-Cazares, Beatriz</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dynamics of Huanglongbing-associated Bacterium Candidatus Liberibacter asiaticus in Citrus aurantifolia Swingle (Mexican Lime)</atitle><jtitle>Pakistan journal of biological sciences</jtitle><addtitle>Pak J Biol Sci</addtitle><date>2017</date><risdate>2017</risdate><volume>20</volume><issue>3</issue><spage>113</spage><epage>123</epage><pages>113-123</pages><issn>1028-8880</issn><abstract>The bacterial disease citrus huanglongbing (HLB), associated with "Candidatus Liberibacter asiaticus" (C.Las) has severely impacted the citrus industry, causing a significant reduction in production and fruit quality. In the present study, it was monitored the C.Las population dynamics in symptomatic, HLB-positive Mexican lime trees (Citrus aurantifolia Swingle) in a tropical, citrus-producing area of Mexico. The objective of this study was to identify the dynamics of the population of huanglongbing-associated bacterium Candidatus Liberibacter asiaticus and its insect vector in Citrus aurantifolia Swingle (Mexican lime).
Leaf samples were collected every 2 months over a period of 26 months for quantification of bacterial titers and young and mature leaves were collected in each season to determine preferential sites of bacterial accumulation. The proportion of living and dead bacterial cells could be determined through the use of quantitative real-time PCR in the presence of ethidium monoazide (EMA-qPCR).
It was observed a lower bacterial titer at high temperatures in the infected trees relative to titers in mild weather, despite a higher accumulation of the insect vector Diaphorina citri in these conditions. This study also revealed seasonal fluctuations in the titers of bacteria in mature leaves when compared to young leaves. No statistically significant correlation between any meteorological variable, C.Las concentration and D. citri population could be drawn.
Although, HLB management strategies have focused on vector control, host tree phenology may be important. The evaluation of citrus phenology, C.Las concentration, ACP population and environmental conditions provides insights into the cyclical, seasonal variations of both the HLB pathogen and its vector. These findings should help in the design of integrative HLB control strategies that take into account the accumulation of the pathogen and the presence of its vector.</abstract><cop>Pakistan</cop><pmid>29023002</pmid><doi>10.3923/pjbs.2017.113.123</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Animals Bacterial Load Citrus aurantiifolia - growth & development Citrus aurantiifolia - microbiology Crops, Agricultural - growth & development Crops, Agricultural - microbiology Disease Vectors Hemiptera - microbiology Host-Pathogen Interactions Mexico Microbial Viability Plant Diseases - microbiology Plant Leaves - growth & development Plant Leaves - microbiology Population Dynamics Rhizobiaceae - genetics Rhizobiaceae - growth & development Seasons Time Factors Trees - growth & development Trees - microbiology Weather |
title | Dynamics of Huanglongbing-associated Bacterium Candidatus Liberibacter asiaticus in Citrus aurantifolia Swingle (Mexican Lime) |
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