Dynamics of Huanglongbing-associated Bacterium Candidatus Liberibacter asiaticus in Citrus aurantifolia Swingle (Mexican Lime)

The bacterial disease citrus huanglongbing (HLB), associated with "Candidatus Liberibacter asiaticus" (C.Las) has severely impacted the citrus industry, causing a significant reduction in production and fruit quality. In the present study, it was monitored the C.Las population dynamics in...

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Veröffentlicht in:Pakistan journal of biological sciences 2017, Vol.20 (3), p.113-123
Hauptverfasser: Abel Lopez-Buenfil, Jose, Abrahan Ramirez-Pool, Jose, Ruiz-Medrano, Roberto, Del Carmen Montes-Horcasitas, Maria, Chavarin-Palacio, Claudio, Moya-Hinojosa, Jesus, Javier Trujillo-Arriaga, Francisco, Carmona, Rosalia Lira, Xoconostle-Cazares, Beatriz
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container_issue 3
container_start_page 113
container_title Pakistan journal of biological sciences
container_volume 20
creator Abel Lopez-Buenfil, Jose
Abrahan Ramirez-Pool, Jose
Ruiz-Medrano, Roberto
Del Carmen Montes-Horcasitas, Maria
Chavarin-Palacio, Claudio
Moya-Hinojosa, Jesus
Javier Trujillo-Arriaga, Francisco
Carmona, Rosalia Lira
Xoconostle-Cazares, Beatriz
description The bacterial disease citrus huanglongbing (HLB), associated with "Candidatus Liberibacter asiaticus" (C.Las) has severely impacted the citrus industry, causing a significant reduction in production and fruit quality. In the present study, it was monitored the C.Las population dynamics in symptomatic, HLB-positive Mexican lime trees (Citrus aurantifolia Swingle) in a tropical, citrus-producing area of Mexico. The objective of this study was to identify the dynamics of the population of huanglongbing-associated bacterium Candidatus Liberibacter asiaticus and its insect vector in Citrus aurantifolia Swingle (Mexican lime). Leaf samples were collected every 2 months over a period of 26 months for quantification of bacterial titers and young and mature leaves were collected in each season to determine preferential sites of bacterial accumulation. The proportion of living and dead bacterial cells could be determined through the use of quantitative real-time PCR in the presence of ethidium monoazide (EMA-qPCR). It was observed a lower bacterial titer at high temperatures in the infected trees relative to titers in mild weather, despite a higher accumulation of the insect vector Diaphorina citri in these conditions. This study also revealed seasonal fluctuations in the titers of bacteria in mature leaves when compared to young leaves. No statistically significant correlation between any meteorological variable, C.Las concentration and D. citri population could be drawn. Although, HLB management strategies have focused on vector control, host tree phenology may be important. The evaluation of citrus phenology, C.Las concentration, ACP population and environmental conditions provides insights into the cyclical, seasonal variations of both the HLB pathogen and its vector. These findings should help in the design of integrative HLB control strategies that take into account the accumulation of the pathogen and the presence of its vector.
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It was observed a lower bacterial titer at high temperatures in the infected trees relative to titers in mild weather, despite a higher accumulation of the insect vector Diaphorina citri in these conditions. This study also revealed seasonal fluctuations in the titers of bacteria in mature leaves when compared to young leaves. No statistically significant correlation between any meteorological variable, C.Las concentration and D. citri population could be drawn. Although, HLB management strategies have focused on vector control, host tree phenology may be important. The evaluation of citrus phenology, C.Las concentration, ACP population and environmental conditions provides insights into the cyclical, seasonal variations of both the HLB pathogen and its vector. 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subjects Animals
Bacterial Load
Citrus aurantiifolia - growth & development
Citrus aurantiifolia - microbiology
Crops, Agricultural - growth & development
Crops, Agricultural - microbiology
Disease Vectors
Hemiptera - microbiology
Host-Pathogen Interactions
Mexico
Microbial Viability
Plant Diseases - microbiology
Plant Leaves - growth & development
Plant Leaves - microbiology
Population Dynamics
Rhizobiaceae - genetics
Rhizobiaceae - growth & development
Seasons
Time Factors
Trees - growth & development
Trees - microbiology
Weather
title Dynamics of Huanglongbing-associated Bacterium Candidatus Liberibacter asiaticus in Citrus aurantifolia Swingle (Mexican Lime)
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