CsGOGAT Is Important in Dynamic Changes of Theanine Content in Postharvest Tea Plant Leaves under Different Temperature and Shading Spreadings
We analyzed the changes of theanine content in postharvest tea leaves under high temperature (38 °C), low temperature (4 °C), and shading spreadings by using ultrahigh-performance liquid chromatography. The differentially expressed proteins (DEPs), CsFd-GOGAT and CsNADH-GOGAT, which are involved in...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2017-11, Vol.65 (44), p.9693-9702 |
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| container_issue | 44 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | Liu, Zhi-Wei Li, Hui Wang, Wen-Li Wu, Zhi-Jun Cui, Xin Zhuang, Jing |
| description | We analyzed the changes of theanine content in postharvest tea leaves under high temperature (38 °C), low temperature (4 °C), and shading spreadings by using ultrahigh-performance liquid chromatography. The differentially expressed proteins (DEPs), CsFd-GOGAT and CsNADH-GOGAT, which are involved in theanine biosynthesis pathway, were identified from the corresponding proteome data. The protein–protein interactions of CsFd-GOGAT and CsNADH-GOGAT, CsTS1, or CsNiR were verified by yeast two-hybrid technology. The expression profiles of 17 genes in theanine metabolism, including CsFd-GOGAT and CsNADH-GOGAT, were analyzed by quantitative real-time polymerase chain reaction. The correlations between the dynamic changes of theanine content and expression profiles of related genes and DEPs were analyzed. This study preliminarily proved the importance of CsGOGAT in dynamic changes of theanine content in postharvest tea leaves during spreading. |
| doi_str_mv | 10.1021/acs.jafc.7b04552 |
| format | Article |
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The differentially expressed proteins (DEPs), CsFd-GOGAT and CsNADH-GOGAT, which are involved in theanine biosynthesis pathway, were identified from the corresponding proteome data. The protein–protein interactions of CsFd-GOGAT and CsNADH-GOGAT, CsTS1, or CsNiR were verified by yeast two-hybrid technology. The expression profiles of 17 genes in theanine metabolism, including CsFd-GOGAT and CsNADH-GOGAT, were analyzed by quantitative real-time polymerase chain reaction. The correlations between the dynamic changes of theanine content and expression profiles of related genes and DEPs were analyzed. This study preliminarily proved the importance of CsGOGAT in dynamic changes of theanine content in postharvest tea leaves during spreading.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/acs.jafc.7b04552</identifier><identifier>PMID: 29020770</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Camellia sinensis - chemistry ; Camellia sinensis - genetics ; Camellia sinensis - growth & development ; Camellia sinensis - metabolism ; Food Handling ; Gene Expression Regulation, Plant ; Glutamates - analysis ; Glutamates - biosynthesis ; Plant Leaves - chemistry ; Plant Leaves - genetics ; Plant Leaves - growth & development ; Plant Leaves - metabolism ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Temperature</subject><ispartof>Journal of agricultural and food chemistry, 2017-11, Vol.65 (44), p.9693-9702</ispartof><rights>Copyright © 2017 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a336t-6341ebdd9ef5417681db2e24f6f48625a86c419e0a053f814315ad851a89fffb3</citedby><cites>FETCH-LOGICAL-a336t-6341ebdd9ef5417681db2e24f6f48625a86c419e0a053f814315ad851a89fffb3</cites><orcidid>0000-0001-6960-5217</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.jafc.7b04552$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.jafc.7b04552$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2763,27075,27923,27924,56737,56787</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29020770$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Zhi-Wei</creatorcontrib><creatorcontrib>Li, Hui</creatorcontrib><creatorcontrib>Wang, Wen-Li</creatorcontrib><creatorcontrib>Wu, Zhi-Jun</creatorcontrib><creatorcontrib>Cui, Xin</creatorcontrib><creatorcontrib>Zhuang, Jing</creatorcontrib><title>CsGOGAT Is Important in Dynamic Changes of Theanine Content in Postharvest Tea Plant Leaves under Different Temperature and Shading Spreadings</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>We analyzed the changes of theanine content in postharvest tea leaves under high temperature (38 °C), low temperature (4 °C), and shading spreadings by using ultrahigh-performance liquid chromatography. The differentially expressed proteins (DEPs), CsFd-GOGAT and CsNADH-GOGAT, which are involved in theanine biosynthesis pathway, were identified from the corresponding proteome data. The protein–protein interactions of CsFd-GOGAT and CsNADH-GOGAT, CsTS1, or CsNiR were verified by yeast two-hybrid technology. The expression profiles of 17 genes in theanine metabolism, including CsFd-GOGAT and CsNADH-GOGAT, were analyzed by quantitative real-time polymerase chain reaction. The correlations between the dynamic changes of theanine content and expression profiles of related genes and DEPs were analyzed. This study preliminarily proved the importance of CsGOGAT in dynamic changes of theanine content in postharvest tea leaves during spreading.</description><subject>Camellia sinensis - chemistry</subject><subject>Camellia sinensis - genetics</subject><subject>Camellia sinensis - growth & development</subject><subject>Camellia sinensis - metabolism</subject><subject>Food Handling</subject><subject>Gene Expression Regulation, Plant</subject><subject>Glutamates - analysis</subject><subject>Glutamates - biosynthesis</subject><subject>Plant Leaves - chemistry</subject><subject>Plant Leaves - genetics</subject><subject>Plant Leaves - growth & development</subject><subject>Plant Leaves - metabolism</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Temperature</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kUFP3DAQha2Kqmxp7z1VPnIgW09iJ84RBbqstBJIpOdokozZoI2T2gkSf4LfjJdduHGa0eh7TzPzGPsFYgkihj_Y-OUjmmaZ1UIqFX9hC1CxiBSAPmELEZhIqxRO2XfvH4UQWmXiGzuNcxGLLBML9lL41e3qsuRrz9f9OLgJ7cQ7y6-eLfZdw4st2gfyfDC83BLazhIvBjvRAbsb_LRF90R-4iUhv9vt9RvCMOGzbcnxq84Ycnu-pH4kh9PsiKNt-f0W284-8PvR0Vvnf7CvBneefh7rGfv397osbqLN7WpdXG4iTJJ0itJEAtVtm5NRErJUQ1vHFEuTGqnTWKFOGwk5CRQqMRpkAgpbrQB1boypkzN2fvAd3fB_DstXfecb2oXtaZh9BbkSEmSWqoCKA9q4wXtHphpd16N7rkBU-xSqkEK1T6E6phAkv4_uc91T-yF4f3sALg7Am3SYnQ3Hfu73CuYhlBw</recordid><startdate>20171108</startdate><enddate>20171108</enddate><creator>Liu, Zhi-Wei</creator><creator>Li, Hui</creator><creator>Wang, Wen-Li</creator><creator>Wu, Zhi-Jun</creator><creator>Cui, Xin</creator><creator>Zhuang, Jing</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6960-5217</orcidid></search><sort><creationdate>20171108</creationdate><title>CsGOGAT Is Important in Dynamic Changes of Theanine Content in Postharvest Tea Plant Leaves under Different Temperature and Shading Spreadings</title><author>Liu, Zhi-Wei ; Li, Hui ; Wang, Wen-Li ; Wu, Zhi-Jun ; Cui, Xin ; Zhuang, Jing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a336t-6341ebdd9ef5417681db2e24f6f48625a86c419e0a053f814315ad851a89fffb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Camellia sinensis - chemistry</topic><topic>Camellia sinensis - genetics</topic><topic>Camellia sinensis - growth & development</topic><topic>Camellia sinensis - metabolism</topic><topic>Food Handling</topic><topic>Gene Expression Regulation, Plant</topic><topic>Glutamates - analysis</topic><topic>Glutamates - biosynthesis</topic><topic>Plant Leaves - chemistry</topic><topic>Plant Leaves - genetics</topic><topic>Plant Leaves - growth & development</topic><topic>Plant Leaves - metabolism</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Zhi-Wei</creatorcontrib><creatorcontrib>Li, Hui</creatorcontrib><creatorcontrib>Wang, Wen-Li</creatorcontrib><creatorcontrib>Wu, Zhi-Jun</creatorcontrib><creatorcontrib>Cui, Xin</creatorcontrib><creatorcontrib>Zhuang, Jing</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Zhi-Wei</au><au>Li, Hui</au><au>Wang, Wen-Li</au><au>Wu, Zhi-Jun</au><au>Cui, Xin</au><au>Zhuang, Jing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CsGOGAT Is Important in Dynamic Changes of Theanine Content in Postharvest Tea Plant Leaves under Different Temperature and Shading Spreadings</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2017-11-08</date><risdate>2017</risdate><volume>65</volume><issue>44</issue><spage>9693</spage><epage>9702</epage><pages>9693-9702</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><abstract>We analyzed the changes of theanine content in postharvest tea leaves under high temperature (38 °C), low temperature (4 °C), and shading spreadings by using ultrahigh-performance liquid chromatography. The differentially expressed proteins (DEPs), CsFd-GOGAT and CsNADH-GOGAT, which are involved in theanine biosynthesis pathway, were identified from the corresponding proteome data. The protein–protein interactions of CsFd-GOGAT and CsNADH-GOGAT, CsTS1, or CsNiR were verified by yeast two-hybrid technology. The expression profiles of 17 genes in theanine metabolism, including CsFd-GOGAT and CsNADH-GOGAT, were analyzed by quantitative real-time polymerase chain reaction. The correlations between the dynamic changes of theanine content and expression profiles of related genes and DEPs were analyzed. This study preliminarily proved the importance of CsGOGAT in dynamic changes of theanine content in postharvest tea leaves during spreading.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>29020770</pmid><doi>10.1021/acs.jafc.7b04552</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-6960-5217</orcidid></addata></record> |
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| ispartof | Journal of agricultural and food chemistry, 2017-11, Vol.65 (44), p.9693-9702 |
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| source | MEDLINE; ACS Publications |
| subjects | Camellia sinensis - chemistry Camellia sinensis - genetics Camellia sinensis - growth & development Camellia sinensis - metabolism Food Handling Gene Expression Regulation, Plant Glutamates - analysis Glutamates - biosynthesis Plant Leaves - chemistry Plant Leaves - genetics Plant Leaves - growth & development Plant Leaves - metabolism Plant Proteins - genetics Plant Proteins - metabolism Temperature |
| title | CsGOGAT Is Important in Dynamic Changes of Theanine Content in Postharvest Tea Plant Leaves under Different Temperature and Shading Spreadings |
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