Effect of UV radiation and its implications on carotenoid pathway in Bixa orellana L
The current study was undertaken to analyse the effect of short-term UV-B and UV-C radiations in provoking carotenoid biosynthesis in Bixa orellana. Seeds of B. orellana were germinated and exposed to the short term UV pre-treatment under controlled environmental condition for 5days. The UV treated...
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| creator | Sankari, M. Hridya, H. Sneha, P. George Priya Doss, C. Ramamoorthy, Siva |
| description | The current study was undertaken to analyse the effect of short-term UV-B and UV-C radiations in provoking carotenoid biosynthesis in Bixa orellana. Seeds of B. orellana were germinated and exposed to the short term UV pre-treatment under controlled environmental condition for 5days. The UV treated young seedlings response in pigment contents; antioxidant enzyme activity and mRNA gene expression level were analysed. The pigment content such as chlorophyll was increased in both UV-B and UV-C treated seedlings, but the total carotenoid level was decreased when compared to the control seedlings this can be attributed to the plant adaptability to survive in a stressed condition. The β-carotene level was increased in UV-B, and UV-C treated young seedlings. No significant changes have occurred in the secondary pigment such as bixin and ABA. The activity of the antioxidant enzymes such as catalase, peroxidase, and superoxide dismutase was significantly increased in UV-B treated seedlings when compared to the UV-C treated seedlings and control. The mRNA expression of the genes involved in bixin biosynthesis pathways such as DXS, PSY, PDS, LCY-β, LCY-ε, CMT, LCD, ADH and CCD genes showed different expression pattern in UV-B and UV-C treated young seedlings. Further we analysed the gene co-expression network to identify the genes which are mainly involved in carotenoid/bixin biosynthesis pathway. Form our findings the CCD, LCY, PDS, ZDS and PSY showed a close interaction. The result of our study shows that the short term UV-B and UV-C radiations induce pigment content, antioxidant enzyme activity and different gene expression pattern allowing the plant to survive in the oxidative stress condition.
•Morphological variation in bixin accumulation in leaves•The UV B and UV C stress increased the β-carotene content in B. orellana.•The antioxidant defense system showed an improved UV B stress resistance.•Differential expression of carotenoid biosynthetic genes |
| doi_str_mv | 10.1016/j.jphotobiol.2017.10.002 |
| format | Article |
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•Morphological variation in bixin accumulation in leaves•The UV B and UV C stress increased the β-carotene content in B. orellana.•The antioxidant defense system showed an improved UV B stress resistance.•Differential expression of carotenoid biosynthetic genes</description><identifier>ISSN: 1011-1344</identifier><identifier>EISSN: 1873-2682</identifier><identifier>DOI: 10.1016/j.jphotobiol.2017.10.002</identifier><identifier>PMID: 28992607</identifier><language>eng</language><publisher>Switzerland: Elsevier B.V</publisher><subject>Abscisic acid ; Adaptability ; Antioxidant assays ; Antioxidants ; beta Carotene - metabolism ; Biosynthesis ; Bixa orellana ; Bixaceae - growth & development ; Bixaceae - metabolism ; Bixin biosynthesis ; Carotenoids - biosynthesis ; Carotenoids - metabolism ; Catalase ; Catalase - genetics ; Catalase - metabolism ; Chlorophyll ; Enzymatic activity ; Enzyme activity ; Gene expression ; Genes ; Germination - radiation effects ; Leaves ; Microscopy, Electron, Scanning ; Morphology ; Oxidative stress ; Oxidoreductases - genetics ; Oxidoreductases - metabolism ; Peroxidase ; Pigment estimation ; Pigmentation - radiation effects ; Plant Leaves - metabolism ; Plant Leaves - radiation effects ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Pretreatment ; Real-Time Polymerase Chain Reaction ; RNA, Plant - isolation & purification ; RNA, Plant - metabolism ; Seedlings ; Seeds ; Seeds - growth & development ; Seeds - metabolism ; Seeds - radiation effects ; Superoxide dismutase ; Superoxide Dismutase - genetics ; Superoxide Dismutase - metabolism ; Ultraviolet radiation ; Ultraviolet Rays ; UV-B and UV-C radiation ; β-Carotene</subject><ispartof>Journal of photochemistry and photobiology. B, Biology, 2017-11, Vol.176, p.136-144</ispartof><rights>2017 Elsevier B.V.</rights><rights>Copyright © 2017 Elsevier B.V. All rights reserved.</rights><rights>Copyright Elsevier BV Nov 2017</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c523t-5e60e3eb4b6ab400b8aaa44611b99ff1f83a6e1183c0f3eef13659da1b874a103</citedby><cites>FETCH-LOGICAL-c523t-5e60e3eb4b6ab400b8aaa44611b99ff1f83a6e1183c0f3eef13659da1b874a103</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jphotobiol.2017.10.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28992607$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sankari, M.</creatorcontrib><creatorcontrib>Hridya, H.</creatorcontrib><creatorcontrib>Sneha, P.</creatorcontrib><creatorcontrib>George Priya Doss, C.</creatorcontrib><creatorcontrib>Ramamoorthy, Siva</creatorcontrib><title>Effect of UV radiation and its implications on carotenoid pathway in Bixa orellana L</title><title>Journal of photochemistry and photobiology. B, Biology</title><addtitle>J Photochem Photobiol B</addtitle><description>The current study was undertaken to analyse the effect of short-term UV-B and UV-C radiations in provoking carotenoid biosynthesis in Bixa orellana. Seeds of B. orellana were germinated and exposed to the short term UV pre-treatment under controlled environmental condition for 5days. The UV treated young seedlings response in pigment contents; antioxidant enzyme activity and mRNA gene expression level were analysed. The pigment content such as chlorophyll was increased in both UV-B and UV-C treated seedlings, but the total carotenoid level was decreased when compared to the control seedlings this can be attributed to the plant adaptability to survive in a stressed condition. The β-carotene level was increased in UV-B, and UV-C treated young seedlings. No significant changes have occurred in the secondary pigment such as bixin and ABA. The activity of the antioxidant enzymes such as catalase, peroxidase, and superoxide dismutase was significantly increased in UV-B treated seedlings when compared to the UV-C treated seedlings and control. The mRNA expression of the genes involved in bixin biosynthesis pathways such as DXS, PSY, PDS, LCY-β, LCY-ε, CMT, LCD, ADH and CCD genes showed different expression pattern in UV-B and UV-C treated young seedlings. Further we analysed the gene co-expression network to identify the genes which are mainly involved in carotenoid/bixin biosynthesis pathway. Form our findings the CCD, LCY, PDS, ZDS and PSY showed a close interaction. The result of our study shows that the short term UV-B and UV-C radiations induce pigment content, antioxidant enzyme activity and different gene expression pattern allowing the plant to survive in the oxidative stress condition.
•Morphological variation in bixin accumulation in leaves•The UV B and UV C stress increased the β-carotene content in B. orellana.•The antioxidant defense system showed an improved UV B stress resistance.•Differential expression of carotenoid biosynthetic genes</description><subject>Abscisic acid</subject><subject>Adaptability</subject><subject>Antioxidant assays</subject><subject>Antioxidants</subject><subject>beta Carotene - metabolism</subject><subject>Biosynthesis</subject><subject>Bixa orellana</subject><subject>Bixaceae - growth & development</subject><subject>Bixaceae - metabolism</subject><subject>Bixin biosynthesis</subject><subject>Carotenoids - biosynthesis</subject><subject>Carotenoids - metabolism</subject><subject>Catalase</subject><subject>Catalase - genetics</subject><subject>Catalase - metabolism</subject><subject>Chlorophyll</subject><subject>Enzymatic activity</subject><subject>Enzyme activity</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Germination - radiation effects</subject><subject>Leaves</subject><subject>Microscopy, Electron, Scanning</subject><subject>Morphology</subject><subject>Oxidative stress</subject><subject>Oxidoreductases - genetics</subject><subject>Oxidoreductases - metabolism</subject><subject>Peroxidase</subject><subject>Pigment estimation</subject><subject>Pigmentation - radiation effects</subject><subject>Plant Leaves - metabolism</subject><subject>Plant Leaves - radiation effects</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Pretreatment</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>RNA, Plant - isolation & purification</subject><subject>RNA, Plant - metabolism</subject><subject>Seedlings</subject><subject>Seeds</subject><subject>Seeds - growth & development</subject><subject>Seeds - metabolism</subject><subject>Seeds - radiation effects</subject><subject>Superoxide dismutase</subject><subject>Superoxide Dismutase - genetics</subject><subject>Superoxide Dismutase - metabolism</subject><subject>Ultraviolet radiation</subject><subject>Ultraviolet Rays</subject><subject>UV-B and UV-C radiation</subject><subject>β-Carotene</subject><issn>1011-1344</issn><issn>1873-2682</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EomXhLyBLXLhk8cSO4xxpVT6klbi0XK2JM1YdZeNgZ4H-e7y7BSQu-GLr9TOe0WPGOIgtCNDvxu243Mc19iFO21pAW-KtEPUTdgmmlVWtTf20nAVABVKpC_Yi51GU1ej2ObuoTdfVWrSX7PbGe3Irj57ffeUJh4BriDPHeeBhzTzslym4U5Z5yR2muNIcw8AXXO9_4AMPM78KP5HHRNOEM_LdS_bM45Tp1eO-YXcfbm6vP1W7Lx8_X7_fVa6p5Vo1pAVJ6lWvsVdC9AYRldIAfdd5D95I1ARgpBNeEnmQuukGhN60CkHIDXt7fndJ8duB8mr3IbvTFBQP2UKnOt1pVZRs2Jt_0DEe0lymK1QrOmUaURfKnCmXYs6JvF1S2GN6sCDs0bwd7V_z9mj-eCNOpa8fGxz6PQ1_Cn-rLsDVGaBi5HugZLMLNDsaQio_YIcY_t_lF0lsmSg</recordid><startdate>201711</startdate><enddate>201711</enddate><creator>Sankari, M.</creator><creator>Hridya, H.</creator><creator>Sneha, P.</creator><creator>George Priya Doss, C.</creator><creator>Ramamoorthy, Siva</creator><general>Elsevier B.V</general><general>Elsevier BV</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TK</scope><scope>7U7</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>201711</creationdate><title>Effect of UV radiation and its implications on carotenoid pathway in Bixa orellana L</title><author>Sankari, M. ; Hridya, H. ; Sneha, P. ; George Priya Doss, C. ; Ramamoorthy, Siva</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c523t-5e60e3eb4b6ab400b8aaa44611b99ff1f83a6e1183c0f3eef13659da1b874a103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Abscisic acid</topic><topic>Adaptability</topic><topic>Antioxidant assays</topic><topic>Antioxidants</topic><topic>beta Carotene - metabolism</topic><topic>Biosynthesis</topic><topic>Bixa orellana</topic><topic>Bixaceae - growth & development</topic><topic>Bixaceae - metabolism</topic><topic>Bixin biosynthesis</topic><topic>Carotenoids - biosynthesis</topic><topic>Carotenoids - metabolism</topic><topic>Catalase</topic><topic>Catalase - genetics</topic><topic>Catalase - metabolism</topic><topic>Chlorophyll</topic><topic>Enzymatic activity</topic><topic>Enzyme activity</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Germination - radiation effects</topic><topic>Leaves</topic><topic>Microscopy, Electron, Scanning</topic><topic>Morphology</topic><topic>Oxidative stress</topic><topic>Oxidoreductases - genetics</topic><topic>Oxidoreductases - metabolism</topic><topic>Peroxidase</topic><topic>Pigment estimation</topic><topic>Pigmentation - radiation effects</topic><topic>Plant Leaves - metabolism</topic><topic>Plant Leaves - radiation effects</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Pretreatment</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>RNA, Plant - isolation & purification</topic><topic>RNA, Plant - metabolism</topic><topic>Seedlings</topic><topic>Seeds</topic><topic>Seeds - growth & development</topic><topic>Seeds - metabolism</topic><topic>Seeds - radiation effects</topic><topic>Superoxide dismutase</topic><topic>Superoxide Dismutase - genetics</topic><topic>Superoxide Dismutase - metabolism</topic><topic>Ultraviolet radiation</topic><topic>Ultraviolet Rays</topic><topic>UV-B and UV-C radiation</topic><topic>β-Carotene</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sankari, M.</creatorcontrib><creatorcontrib>Hridya, H.</creatorcontrib><creatorcontrib>Sneha, P.</creatorcontrib><creatorcontrib>George Priya Doss, C.</creatorcontrib><creatorcontrib>Ramamoorthy, Siva</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of photochemistry and photobiology. B, Biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sankari, M.</au><au>Hridya, H.</au><au>Sneha, P.</au><au>George Priya Doss, C.</au><au>Ramamoorthy, Siva</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of UV radiation and its implications on carotenoid pathway in Bixa orellana L</atitle><jtitle>Journal of photochemistry and photobiology. B, Biology</jtitle><addtitle>J Photochem Photobiol B</addtitle><date>2017-11</date><risdate>2017</risdate><volume>176</volume><spage>136</spage><epage>144</epage><pages>136-144</pages><issn>1011-1344</issn><eissn>1873-2682</eissn><abstract>The current study was undertaken to analyse the effect of short-term UV-B and UV-C radiations in provoking carotenoid biosynthesis in Bixa orellana. Seeds of B. orellana were germinated and exposed to the short term UV pre-treatment under controlled environmental condition for 5days. The UV treated young seedlings response in pigment contents; antioxidant enzyme activity and mRNA gene expression level were analysed. The pigment content such as chlorophyll was increased in both UV-B and UV-C treated seedlings, but the total carotenoid level was decreased when compared to the control seedlings this can be attributed to the plant adaptability to survive in a stressed condition. The β-carotene level was increased in UV-B, and UV-C treated young seedlings. No significant changes have occurred in the secondary pigment such as bixin and ABA. The activity of the antioxidant enzymes such as catalase, peroxidase, and superoxide dismutase was significantly increased in UV-B treated seedlings when compared to the UV-C treated seedlings and control. The mRNA expression of the genes involved in bixin biosynthesis pathways such as DXS, PSY, PDS, LCY-β, LCY-ε, CMT, LCD, ADH and CCD genes showed different expression pattern in UV-B and UV-C treated young seedlings. Further we analysed the gene co-expression network to identify the genes which are mainly involved in carotenoid/bixin biosynthesis pathway. Form our findings the CCD, LCY, PDS, ZDS and PSY showed a close interaction. The result of our study shows that the short term UV-B and UV-C radiations induce pigment content, antioxidant enzyme activity and different gene expression pattern allowing the plant to survive in the oxidative stress condition.
•Morphological variation in bixin accumulation in leaves•The UV B and UV C stress increased the β-carotene content in B. orellana.•The antioxidant defense system showed an improved UV B stress resistance.•Differential expression of carotenoid biosynthetic genes</abstract><cop>Switzerland</cop><pub>Elsevier B.V</pub><pmid>28992607</pmid><doi>10.1016/j.jphotobiol.2017.10.002</doi><tpages>9</tpages></addata></record> |
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| ispartof | Journal of photochemistry and photobiology. B, Biology, 2017-11, Vol.176, p.136-144 |
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| subjects | Abscisic acid Adaptability Antioxidant assays Antioxidants beta Carotene - metabolism Biosynthesis Bixa orellana Bixaceae - growth & development Bixaceae - metabolism Bixin biosynthesis Carotenoids - biosynthesis Carotenoids - metabolism Catalase Catalase - genetics Catalase - metabolism Chlorophyll Enzymatic activity Enzyme activity Gene expression Genes Germination - radiation effects Leaves Microscopy, Electron, Scanning Morphology Oxidative stress Oxidoreductases - genetics Oxidoreductases - metabolism Peroxidase Pigment estimation Pigmentation - radiation effects Plant Leaves - metabolism Plant Leaves - radiation effects Plant Proteins - genetics Plant Proteins - metabolism Pretreatment Real-Time Polymerase Chain Reaction RNA, Plant - isolation & purification RNA, Plant - metabolism Seedlings Seeds Seeds - growth & development Seeds - metabolism Seeds - radiation effects Superoxide dismutase Superoxide Dismutase - genetics Superoxide Dismutase - metabolism Ultraviolet radiation Ultraviolet Rays UV-B and UV-C radiation β-Carotene |
| title | Effect of UV radiation and its implications on carotenoid pathway in Bixa orellana L |
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