Does microbial nitrogen contamination affect the estimation of crude protein degradability of concentrate feeds?
The effects of microbial contamination (MC) on CP degradability of concentrate feeds are still controversial. Therefore, the aim of this study was to use N to estimate the impact of MC on estimations of CP fractions (the soluble fraction of CP [a], the insoluble but potentially degradable fraction o...
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| Veröffentlicht in: | Journal of animal science 2017-09, Vol.95 (9), p.4164-4171 |
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| creator | Menezes, A C B Filho, S C Valadares Rotta, P P Santos, S A Pacheco, M V C Silva, B C Pucetti, P Alhadas, H M Detmann, E Caton, J S |
| description | The effects of microbial contamination (MC) on CP degradability of concentrate feeds are still controversial. Therefore, the aim of this study was to use N to estimate the impact of MC on estimations of CP fractions (the soluble fraction of CP [a], the insoluble but potentially degradable fraction of CP [b], and the rate of digestion of fraction b [kd]) of concentrate feeds. Twelve types of feed were evaluated: 6 energy concentrates-wheat bran ( L.), rice meal ( L.), ground corn ( L.), ground sorghum ( Pers.), ground corn cob ( L.), and soybean hulls [ (L.) Merr.]-and 6 protein concentrates-cottonseed meal ( L.), soybean meal [ (L.) Merr.], ground bean ( L.), peanut meal ( L.), sunflower meal ( L.), and corn gluten meal ( L.). The feeds were divided into 4 groups and were incubated in the rumen of 4 crossbred bulls. The samples were incubated for 0, 2, 4, 8, 16, 24, 48, and 72 h. To estimate the MC of the incubated residues, the ruminal bacteria were labeled with N via continuous intraruminal infusion of (NH)SO. There was no difference ( = 0.738) between corrected and uncorrected parameters a, b, and kd for all feeds that were evaluated. All of the feed tests followed an exponential model of degradation, and the model fitted well to the data, except for corn gluten meal, probably because the maximum incubation time that was used (72 h) was not long enough to allow for an accurate estimation of the degradation profile. Therefore, correction of ruminal protein degradation to MC is irrelevant with regards to the concentrates that were studied. |
| doi_str_mv | 10.2527/jas2017.1699 |
| format | Article |
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Therefore, the aim of this study was to use N to estimate the impact of MC on estimations of CP fractions (the soluble fraction of CP [a], the insoluble but potentially degradable fraction of CP [b], and the rate of digestion of fraction b [kd]) of concentrate feeds. Twelve types of feed were evaluated: 6 energy concentrates-wheat bran ( L.), rice meal ( L.), ground corn ( L.), ground sorghum ( Pers.), ground corn cob ( L.), and soybean hulls [ (L.) Merr.]-and 6 protein concentrates-cottonseed meal ( L.), soybean meal [ (L.) Merr.], ground bean ( L.), peanut meal ( L.), sunflower meal ( L.), and corn gluten meal ( L.). The feeds were divided into 4 groups and were incubated in the rumen of 4 crossbred bulls. The samples were incubated for 0, 2, 4, 8, 16, 24, 48, and 72 h. To estimate the MC of the incubated residues, the ruminal bacteria were labeled with N via continuous intraruminal infusion of (NH)SO. There was no difference ( = 0.738) between corrected and uncorrected parameters a, b, and kd for all feeds that were evaluated. All of the feed tests followed an exponential model of degradation, and the model fitted well to the data, except for corn gluten meal, probably because the maximum incubation time that was used (72 h) was not long enough to allow for an accurate estimation of the degradation profile. Therefore, correction of ruminal protein degradation to MC is irrelevant with regards to the concentrates that were studied.</description><identifier>ISSN: 1525-3163</identifier><identifier>ISSN: 0021-8812</identifier><identifier>EISSN: 1525-3163</identifier><identifier>DOI: 10.2527/jas2017.1699</identifier><identifier>PMID: 28992006</identifier><language>eng</language><publisher>United States: Oxford University Press</publisher><subject>Animal Feed - analysis ; Animal Feed - microbiology ; Animals ; Bacteria ; Bacteria - metabolism ; Biodegradation ; Cattle - physiology ; Contamination ; Corn ; Cottonseed meal ; Degradability ; Degradation ; Dietary Proteins - metabolism ; Digestion ; Feeds ; Gluten ; Glycine ; Hulls ; Male ; Microbial contamination ; Microorganisms ; Nitrogen ; Nitrogen - analysis ; Nitrogen - metabolism ; Nitrogen Isotopes - analysis ; Oryza sativa ; Phaseolus vulgaris ; Plant Proteins - metabolism ; Proteins ; Proteolysis ; Rumen - metabolism ; Sorghum ; Triticum aestivum ; Wheat ; Wheat bran ; Zea mays</subject><ispartof>Journal of animal science, 2017-09, Vol.95 (9), p.4164-4171</ispartof><rights>Copyright American Society of Animal Science Sep 2017</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1646-b0ed46f6ae770fde84bdcea9edaecef1402accf45b64739e0aa1695b80afe02f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28992006$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Menezes, A C B</creatorcontrib><creatorcontrib>Filho, S C Valadares</creatorcontrib><creatorcontrib>Rotta, P P</creatorcontrib><creatorcontrib>Santos, S A</creatorcontrib><creatorcontrib>Pacheco, M V C</creatorcontrib><creatorcontrib>Silva, B C</creatorcontrib><creatorcontrib>Pucetti, P</creatorcontrib><creatorcontrib>Alhadas, H M</creatorcontrib><creatorcontrib>Detmann, E</creatorcontrib><creatorcontrib>Caton, J S</creatorcontrib><title>Does microbial nitrogen contamination affect the estimation of crude protein degradability of concentrate feeds?</title><title>Journal of animal science</title><addtitle>J Anim Sci</addtitle><description>The effects of microbial contamination (MC) on CP degradability of concentrate feeds are still controversial. Therefore, the aim of this study was to use N to estimate the impact of MC on estimations of CP fractions (the soluble fraction of CP [a], the insoluble but potentially degradable fraction of CP [b], and the rate of digestion of fraction b [kd]) of concentrate feeds. Twelve types of feed were evaluated: 6 energy concentrates-wheat bran ( L.), rice meal ( L.), ground corn ( L.), ground sorghum ( Pers.), ground corn cob ( L.), and soybean hulls [ (L.) Merr.]-and 6 protein concentrates-cottonseed meal ( L.), soybean meal [ (L.) Merr.], ground bean ( L.), peanut meal ( L.), sunflower meal ( L.), and corn gluten meal ( L.). The feeds were divided into 4 groups and were incubated in the rumen of 4 crossbred bulls. The samples were incubated for 0, 2, 4, 8, 16, 24, 48, and 72 h. To estimate the MC of the incubated residues, the ruminal bacteria were labeled with N via continuous intraruminal infusion of (NH)SO. There was no difference ( = 0.738) between corrected and uncorrected parameters a, b, and kd for all feeds that were evaluated. All of the feed tests followed an exponential model of degradation, and the model fitted well to the data, except for corn gluten meal, probably because the maximum incubation time that was used (72 h) was not long enough to allow for an accurate estimation of the degradation profile. Therefore, correction of ruminal protein degradation to MC is irrelevant with regards to the concentrates that were studied.</description><subject>Animal Feed - analysis</subject><subject>Animal Feed - microbiology</subject><subject>Animals</subject><subject>Bacteria</subject><subject>Bacteria - metabolism</subject><subject>Biodegradation</subject><subject>Cattle - physiology</subject><subject>Contamination</subject><subject>Corn</subject><subject>Cottonseed meal</subject><subject>Degradability</subject><subject>Degradation</subject><subject>Dietary Proteins - metabolism</subject><subject>Digestion</subject><subject>Feeds</subject><subject>Gluten</subject><subject>Glycine</subject><subject>Hulls</subject><subject>Male</subject><subject>Microbial contamination</subject><subject>Microorganisms</subject><subject>Nitrogen</subject><subject>Nitrogen - analysis</subject><subject>Nitrogen - metabolism</subject><subject>Nitrogen Isotopes - analysis</subject><subject>Oryza sativa</subject><subject>Phaseolus vulgaris</subject><subject>Plant Proteins - metabolism</subject><subject>Proteins</subject><subject>Proteolysis</subject><subject>Rumen - metabolism</subject><subject>Sorghum</subject><subject>Triticum aestivum</subject><subject>Wheat</subject><subject>Wheat bran</subject><subject>Zea mays</subject><issn>1525-3163</issn><issn>0021-8812</issn><issn>1525-3163</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkUFrGzEQRkVpaRI3t5yLoJccYkfS7mqtUylO0gQMvaTnZVYaOTK7kiNpD_73lWM3lJ5GjB7Dx_sIueJsIRrR3m4hCcbbBZdKfSDnvBHNvOKy-vjP-4xcpLRljItGNZ_JmVgqJRiT52R3FzDR0ekYegcD9S7HsEFPdfAZRuchu-ApWIs60_yCFFN243EbLNVxMkh3MWR0nhrcRDDQu8Hl_dt38Bp9jpCRWkSTvn8hnywMCS9Pc0Z-P9w_rx7n618_n1Y_1nPNZS3nPUNTSysB25ZZg8u6NxpBoQHUaHnNBGht66aXdVspZABFQNMvGVhkwlYzcn28W7K9TiV0N7qkcRjAY5hSx1WtpKqEEAX99h-6DVP0Jd2BkqJtiuBC3RypoiqliLbbxSIi7jvOukMT3amJ7tBEwb-ejk79iOYd_qu--gMxkYa8</recordid><startdate>201709</startdate><enddate>201709</enddate><creator>Menezes, A C B</creator><creator>Filho, S C Valadares</creator><creator>Rotta, P P</creator><creator>Santos, S A</creator><creator>Pacheco, M V C</creator><creator>Silva, B C</creator><creator>Pucetti, P</creator><creator>Alhadas, H M</creator><creator>Detmann, E</creator><creator>Caton, J S</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RQ</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M7P</scope><scope>M7S</scope><scope>MBDVC</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>S0X</scope><scope>U9A</scope><scope>7X8</scope></search><sort><creationdate>201709</creationdate><title>Does microbial nitrogen contamination affect the estimation of crude protein degradability of concentrate feeds?</title><author>Menezes, A C B ; Filho, S C Valadares ; Rotta, P P ; Santos, S A ; Pacheco, M V C ; Silva, B C ; Pucetti, P ; Alhadas, H M ; Detmann, E ; Caton, J S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1646-b0ed46f6ae770fde84bdcea9edaecef1402accf45b64739e0aa1695b80afe02f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animal Feed - 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Academic</collection><jtitle>Journal of animal science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Menezes, A C B</au><au>Filho, S C Valadares</au><au>Rotta, P P</au><au>Santos, S A</au><au>Pacheco, M V C</au><au>Silva, B C</au><au>Pucetti, P</au><au>Alhadas, H M</au><au>Detmann, E</au><au>Caton, J S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Does microbial nitrogen contamination affect the estimation of crude protein degradability of concentrate feeds?</atitle><jtitle>Journal of animal science</jtitle><addtitle>J Anim Sci</addtitle><date>2017-09</date><risdate>2017</risdate><volume>95</volume><issue>9</issue><spage>4164</spage><epage>4171</epage><pages>4164-4171</pages><issn>1525-3163</issn><issn>0021-8812</issn><eissn>1525-3163</eissn><abstract>The effects of microbial contamination (MC) on CP degradability of concentrate feeds are still controversial. Therefore, the aim of this study was to use N to estimate the impact of MC on estimations of CP fractions (the soluble fraction of CP [a], the insoluble but potentially degradable fraction of CP [b], and the rate of digestion of fraction b [kd]) of concentrate feeds. Twelve types of feed were evaluated: 6 energy concentrates-wheat bran ( L.), rice meal ( L.), ground corn ( L.), ground sorghum ( Pers.), ground corn cob ( L.), and soybean hulls [ (L.) Merr.]-and 6 protein concentrates-cottonseed meal ( L.), soybean meal [ (L.) Merr.], ground bean ( L.), peanut meal ( L.), sunflower meal ( L.), and corn gluten meal ( L.). The feeds were divided into 4 groups and were incubated in the rumen of 4 crossbred bulls. The samples were incubated for 0, 2, 4, 8, 16, 24, 48, and 72 h. To estimate the MC of the incubated residues, the ruminal bacteria were labeled with N via continuous intraruminal infusion of (NH)SO. There was no difference ( = 0.738) between corrected and uncorrected parameters a, b, and kd for all feeds that were evaluated. All of the feed tests followed an exponential model of degradation, and the model fitted well to the data, except for corn gluten meal, probably because the maximum incubation time that was used (72 h) was not long enough to allow for an accurate estimation of the degradation profile. Therefore, correction of ruminal protein degradation to MC is irrelevant with regards to the concentrates that were studied.</abstract><cop>United States</cop><pub>Oxford University Press</pub><pmid>28992006</pmid><doi>10.2527/jas2017.1699</doi><tpages>8</tpages></addata></record> |
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| subjects | Animal Feed - analysis Animal Feed - microbiology Animals Bacteria Bacteria - metabolism Biodegradation Cattle - physiology Contamination Corn Cottonseed meal Degradability Degradation Dietary Proteins - metabolism Digestion Feeds Gluten Glycine Hulls Male Microbial contamination Microorganisms Nitrogen Nitrogen - analysis Nitrogen - metabolism Nitrogen Isotopes - analysis Oryza sativa Phaseolus vulgaris Plant Proteins - metabolism Proteins Proteolysis Rumen - metabolism Sorghum Triticum aestivum Wheat Wheat bran Zea mays |
| title | Does microbial nitrogen contamination affect the estimation of crude protein degradability of concentrate feeds? |
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