Structural Basis for Calcium-induced Inhibition of Rhodopsin Kinase by Recoverin

Structural Basis for Calcium-induced Inhibition of Rhodopsin Kinase by Recoverin

Recoverin, a member of the neuronal calcium sensor branch of the EF-hand superfamily, serves as a calcium sensor that regulates rhodopsin kinase (RK) activity in retinal rod cells. We report here the NMR structure of Ca2+-bound recoverin bound to a functional N-terminal fragment of rhodopsin kinase...

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Veröffentlicht in:The Journal of biological chemistry 2006-12, Vol.281 (48), p.37237-37245
Hauptverfasser: Ames, James B., Levay, Konstantin, Wingard, Jennifer N., Lusin, Jacqueline D., Slepak, Vladlen Z.
Format: Artikel
Sprache:eng
Schlagworte:
Amino Acid Sequence
Animals
Binding Sites
Calcium - chemistry
Cattle
Crystallography, X-Ray
Dose-Response Relationship, Drug
G-Protein-Coupled Receptor Kinase 1 - chemistry
G-Protein-Coupled Receptor Kinase 1 - physiology
Magnetic Resonance Spectroscopy
Molecular Conformation
Molecular Sequence Data
Mutagenesis, Site-Directed
Neurons - metabolism
Protein Binding
Recoverin - chemistry
Rhodopsin - chemistry
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Zusammenfassung:Recoverin, a member of the neuronal calcium sensor branch of the EF-hand superfamily, serves as a calcium sensor that regulates rhodopsin kinase (RK) activity in retinal rod cells. We report here the NMR structure of Ca2+-bound recoverin bound to a functional N-terminal fragment of rhodopsin kinase (residues 1-25, called RK25). The overall main-chain structure of recoverin in the complex is similar to structures of Ca2+-bound recoverin in the absence of target (
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M606913200