A new approach for determination of enzyme kinetic constants using response surface methodology

A statistical approach called response surface methodology (RSM) is used for the prediction of the kinetic constants of glucose oxidase (GOx) as a function of reaction temperature and pH. Lineweaver–Burk transformation of the Michaelis–Menten equation was utilized as the integral part of the RSM alg...

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Veröffentlicht in:	Biochemical engineering journal 2005-08, Vol.25 (1), p.55-62
1. Verfasser:	BOYACI, Ismail Hakki
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Enzymes Fundamental and applied biological sciences. Psychology Glucose Glucose oxidase Kinetic constants Response surface methodology
Online-Zugang:	Volltext
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