Low-power laser irradiation inhibits PDGF-BB-induced migration and proliferation via apoptotic cell death in vascular smooth muscle cells
Vascular restenosis after injury of blood vessel has been implicated in various responses including apoptosis, migration, and proliferation in vascular smooth muscle cells (VSMCs) stimulated by diverse growth factors underlying platelet-derived growth factor (PDGF). Previous studies evaluated the ef...
Gespeichert in:
| Veröffentlicht in: | Lasers in medical science 2017-12, Vol.32 (9), p.2121-2127 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2127 |
|---|---|
| container_issue | 9 |
| container_start_page | 2121 |
| container_title | Lasers in medical science |
| container_volume | 32 |
| creator | Baek, Suji Lee, Kang Pa Cui, Long Ryu, Yunkyoung Hong, Jung Min Kim, Junghwan Jung, Seung Hyo Bae, Young Min Won, Kyung Jong Kim, Bokyung |
| description | Vascular restenosis after injury of blood vessel has been implicated in various responses including apoptosis, migration, and proliferation in vascular smooth muscle cells (VSMCs) stimulated by diverse growth factors underlying platelet-derived growth factor (PDGF). Previous studies evaluated the effects of low-power laser (LPL) irradiation over various wavelength ranges on VSMC events in normal and pathologic states. However, whether VSMC responses are affected by LPL irradiation remains unclear. The purpose of this study is to explore the effects of LPL (green diode laser 532-nm pulsed wave of 300 mW at a spot diameter of 1 mm) irradiation on the responses, apoptosis, migration, and proliferation of VSMCs. The effect of LPL irradiation was tested on VSMCs through cytotoxicity, proliferation, migration, and apoptotic assays. Aortic ring assay was used to assess the effect of LPL irradiation on aortic sprout outgrowth. Protein expression levels were determined by western blotting. LPL irradiation did not affect VSMC viability but slightly attenuated PDGF-BB-induced proliferation in VSMCs. In addition, LPL irradiation inhibited PDGF-BB-evoked migration of VSMCs. Aortic sprout outgrowth in response to PDGF-BB was diminished in cells treated with LPL. In contrast, LPL irradiation evoked apoptosis in VSMCs in the presence of PDGF-BB. Similarly, activation of caspase-3 and Bax, as well as p38 mitogen-activated protein kinase (MAPK), in VSMCs treated with PDGF-BB was enhanced by exposure to LPL. These findings indicate that LPL irradiation induces vascular apoptosis via p38 MAPK activation and simultaneously inhibits VSMC proliferation and migration in response to PDGF-BB. |
| doi_str_mv | 10.1007/s10103-017-2338-z |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1948757430</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1948757430</sourcerecordid><originalsourceid>FETCH-LOGICAL-c372t-1ef8e7ae7e41db0ef454ff70b7b748543998951ed0d8b836c86d52c70d2af0873</originalsourceid><addsrcrecordid>eNp1kcFu1TAQRS1ERR-FD2CDLLFh4zKOndhZ0pa2SE9qF0ViZznxpHWVxMFOWtE_4K_xIw-EkNjY0vjM9Z25hLzhcMwB1IfEgYNgwBUrhNDs6RnZcClKVoH8-pxsoKg003XBD8nLlO4hgxUXL8hhoWstKq025Mc2PLIpPGKkvU359DFa5-3sw0j9eOcbPyd6fXZxzk5OmB_d0qKjg7-NK2JHR6cYet_hvvLgLbVTmOYw-5a22PfUoZ3vshp9sKldehtpGkLIpWFJbY-_oPSKHHS2T_h6fx-RL-efbk4v2fbq4vPpxy1rhSpmxrHTqCwqlNw1gJ0sZdcpaFSjpC6lqGtdlxwdON3kIVtdubJoFbjCdqCVOCLvV91s-9uCaTaDTzsHdsSwJMNrqVWppICMvvsHvQ9LHLO7TFVcSdAgM8VXqo0hpYidmaIfbPxuOJhdTmbNyeT1m11O5in3vN0rL82A7k_H72AyUKxAyk_jLca_vv6v6k-7Qp_8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1961740804</pqid></control><display><type>article</type><title>Low-power laser irradiation inhibits PDGF-BB-induced migration and proliferation via apoptotic cell death in vascular smooth muscle cells</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Baek, Suji ; Lee, Kang Pa ; Cui, Long ; Ryu, Yunkyoung ; Hong, Jung Min ; Kim, Junghwan ; Jung, Seung Hyo ; Bae, Young Min ; Won, Kyung Jong ; Kim, Bokyung</creator><creatorcontrib>Baek, Suji ; Lee, Kang Pa ; Cui, Long ; Ryu, Yunkyoung ; Hong, Jung Min ; Kim, Junghwan ; Jung, Seung Hyo ; Bae, Young Min ; Won, Kyung Jong ; Kim, Bokyung</creatorcontrib><description>Vascular restenosis after injury of blood vessel has been implicated in various responses including apoptosis, migration, and proliferation in vascular smooth muscle cells (VSMCs) stimulated by diverse growth factors underlying platelet-derived growth factor (PDGF). Previous studies evaluated the effects of low-power laser (LPL) irradiation over various wavelength ranges on VSMC events in normal and pathologic states. However, whether VSMC responses are affected by LPL irradiation remains unclear. The purpose of this study is to explore the effects of LPL (green diode laser 532-nm pulsed wave of 300 mW at a spot diameter of 1 mm) irradiation on the responses, apoptosis, migration, and proliferation of VSMCs. The effect of LPL irradiation was tested on VSMCs through cytotoxicity, proliferation, migration, and apoptotic assays. Aortic ring assay was used to assess the effect of LPL irradiation on aortic sprout outgrowth. Protein expression levels were determined by western blotting. LPL irradiation did not affect VSMC viability but slightly attenuated PDGF-BB-induced proliferation in VSMCs. In addition, LPL irradiation inhibited PDGF-BB-evoked migration of VSMCs. Aortic sprout outgrowth in response to PDGF-BB was diminished in cells treated with LPL. In contrast, LPL irradiation evoked apoptosis in VSMCs in the presence of PDGF-BB. Similarly, activation of caspase-3 and Bax, as well as p38 mitogen-activated protein kinase (MAPK), in VSMCs treated with PDGF-BB was enhanced by exposure to LPL. These findings indicate that LPL irradiation induces vascular apoptosis via p38 MAPK activation and simultaneously inhibits VSMC proliferation and migration in response to PDGF-BB.</description><identifier>ISSN: 0268-8921</identifier><identifier>EISSN: 1435-604X</identifier><identifier>DOI: 10.1007/s10103-017-2338-z</identifier><identifier>PMID: 28983687</identifier><language>eng</language><publisher>London: Springer London</publisher><subject>Activation ; Animals ; Aorta ; Aorta - cytology ; Apoptosis ; Apoptosis - drug effects ; BAX protein ; Blood vessels ; Caspase ; Caspase-3 ; Cell adhesion & migration ; Cell death ; Cell Movement - drug effects ; Cell proliferation ; Cell Proliferation - drug effects ; Cells, Cultured ; Cytotoxicity ; Dentistry ; Growth factors ; Irradiation ; Kinases ; Lasers ; Low-Level Light Therapy ; Male ; MAP kinase ; Medicine ; Medicine & Public Health ; Muscle, Smooth, Vascular - pathology ; Myocytes, Smooth Muscle - drug effects ; Myocytes, Smooth Muscle - pathology ; Myocytes, Smooth Muscle - radiation effects ; Optical Devices ; Optics ; Original Article ; Photonics ; Platelet-derived growth factor ; Platelet-derived growth factor BB ; Protein kinase ; Proto-Oncogene Proteins c-sis - pharmacology ; Quantum Optics ; Rats, Sprague-Dawley ; Restenosis ; Smooth muscle ; Toxicity testing ; Viability ; Western blotting</subject><ispartof>Lasers in medical science, 2017-12, Vol.32 (9), p.2121-2127</ispartof><rights>Springer-Verlag London Ltd. 2017</rights><rights>Lasers in Medical Science is a copyright of Springer, (2017). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-1ef8e7ae7e41db0ef454ff70b7b748543998951ed0d8b836c86d52c70d2af0873</citedby><cites>FETCH-LOGICAL-c372t-1ef8e7ae7e41db0ef454ff70b7b748543998951ed0d8b836c86d52c70d2af0873</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10103-017-2338-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10103-017-2338-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28983687$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Baek, Suji</creatorcontrib><creatorcontrib>Lee, Kang Pa</creatorcontrib><creatorcontrib>Cui, Long</creatorcontrib><creatorcontrib>Ryu, Yunkyoung</creatorcontrib><creatorcontrib>Hong, Jung Min</creatorcontrib><creatorcontrib>Kim, Junghwan</creatorcontrib><creatorcontrib>Jung, Seung Hyo</creatorcontrib><creatorcontrib>Bae, Young Min</creatorcontrib><creatorcontrib>Won, Kyung Jong</creatorcontrib><creatorcontrib>Kim, Bokyung</creatorcontrib><title>Low-power laser irradiation inhibits PDGF-BB-induced migration and proliferation via apoptotic cell death in vascular smooth muscle cells</title><title>Lasers in medical science</title><addtitle>Lasers Med Sci</addtitle><addtitle>Lasers Med Sci</addtitle><description>Vascular restenosis after injury of blood vessel has been implicated in various responses including apoptosis, migration, and proliferation in vascular smooth muscle cells (VSMCs) stimulated by diverse growth factors underlying platelet-derived growth factor (PDGF). Previous studies evaluated the effects of low-power laser (LPL) irradiation over various wavelength ranges on VSMC events in normal and pathologic states. However, whether VSMC responses are affected by LPL irradiation remains unclear. The purpose of this study is to explore the effects of LPL (green diode laser 532-nm pulsed wave of 300 mW at a spot diameter of 1 mm) irradiation on the responses, apoptosis, migration, and proliferation of VSMCs. The effect of LPL irradiation was tested on VSMCs through cytotoxicity, proliferation, migration, and apoptotic assays. Aortic ring assay was used to assess the effect of LPL irradiation on aortic sprout outgrowth. Protein expression levels were determined by western blotting. LPL irradiation did not affect VSMC viability but slightly attenuated PDGF-BB-induced proliferation in VSMCs. In addition, LPL irradiation inhibited PDGF-BB-evoked migration of VSMCs. Aortic sprout outgrowth in response to PDGF-BB was diminished in cells treated with LPL. In contrast, LPL irradiation evoked apoptosis in VSMCs in the presence of PDGF-BB. Similarly, activation of caspase-3 and Bax, as well as p38 mitogen-activated protein kinase (MAPK), in VSMCs treated with PDGF-BB was enhanced by exposure to LPL. These findings indicate that LPL irradiation induces vascular apoptosis via p38 MAPK activation and simultaneously inhibits VSMC proliferation and migration in response to PDGF-BB.</description><subject>Activation</subject><subject>Animals</subject><subject>Aorta</subject><subject>Aorta - cytology</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>BAX protein</subject><subject>Blood vessels</subject><subject>Caspase</subject><subject>Caspase-3</subject><subject>Cell adhesion & migration</subject><subject>Cell death</subject><subject>Cell Movement - drug effects</subject><subject>Cell proliferation</subject><subject>Cell Proliferation - drug effects</subject><subject>Cells, Cultured</subject><subject>Cytotoxicity</subject><subject>Dentistry</subject><subject>Growth factors</subject><subject>Irradiation</subject><subject>Kinases</subject><subject>Lasers</subject><subject>Low-Level Light Therapy</subject><subject>Male</subject><subject>MAP kinase</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Muscle, Smooth, Vascular - pathology</subject><subject>Myocytes, Smooth Muscle - drug effects</subject><subject>Myocytes, Smooth Muscle - pathology</subject><subject>Myocytes, Smooth Muscle - radiation effects</subject><subject>Optical Devices</subject><subject>Optics</subject><subject>Original Article</subject><subject>Photonics</subject><subject>Platelet-derived growth factor</subject><subject>Platelet-derived growth factor BB</subject><subject>Protein kinase</subject><subject>Proto-Oncogene Proteins c-sis - pharmacology</subject><subject>Quantum Optics</subject><subject>Rats, Sprague-Dawley</subject><subject>Restenosis</subject><subject>Smooth muscle</subject><subject>Toxicity testing</subject><subject>Viability</subject><subject>Western blotting</subject><issn>0268-8921</issn><issn>1435-604X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kcFu1TAQRS1ERR-FD2CDLLFh4zKOndhZ0pa2SE9qF0ViZznxpHWVxMFOWtE_4K_xIw-EkNjY0vjM9Z25hLzhcMwB1IfEgYNgwBUrhNDs6RnZcClKVoH8-pxsoKg003XBD8nLlO4hgxUXL8hhoWstKq025Mc2PLIpPGKkvU359DFa5-3sw0j9eOcbPyd6fXZxzk5OmB_d0qKjg7-NK2JHR6cYet_hvvLgLbVTmOYw-5a22PfUoZ3vshp9sKldehtpGkLIpWFJbY-_oPSKHHS2T_h6fx-RL-efbk4v2fbq4vPpxy1rhSpmxrHTqCwqlNw1gJ0sZdcpaFSjpC6lqGtdlxwdON3kIVtdubJoFbjCdqCVOCLvV91s-9uCaTaDTzsHdsSwJMNrqVWppICMvvsHvQ9LHLO7TFVcSdAgM8VXqo0hpYidmaIfbPxuOJhdTmbNyeT1m11O5in3vN0rL82A7k_H72AyUKxAyk_jLca_vv6v6k-7Qp_8</recordid><startdate>20171201</startdate><enddate>20171201</enddate><creator>Baek, Suji</creator><creator>Lee, Kang Pa</creator><creator>Cui, Long</creator><creator>Ryu, Yunkyoung</creator><creator>Hong, Jung Min</creator><creator>Kim, Junghwan</creator><creator>Jung, Seung Hyo</creator><creator>Bae, Young Min</creator><creator>Won, Kyung Jong</creator><creator>Kim, Bokyung</creator><general>Springer London</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7RV</scope><scope>7SP</scope><scope>7U5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H8D</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>L7M</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20171201</creationdate><title>Low-power laser irradiation inhibits PDGF-BB-induced migration and proliferation via apoptotic cell death in vascular smooth muscle cells</title><author>Baek, Suji ; Lee, Kang Pa ; Cui, Long ; Ryu, Yunkyoung ; Hong, Jung Min ; Kim, Junghwan ; Jung, Seung Hyo ; Bae, Young Min ; Won, Kyung Jong ; Kim, Bokyung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-1ef8e7ae7e41db0ef454ff70b7b748543998951ed0d8b836c86d52c70d2af0873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Activation</topic><topic>Animals</topic><topic>Aorta</topic><topic>Aorta - cytology</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>BAX protein</topic><topic>Blood vessels</topic><topic>Caspase</topic><topic>Caspase-3</topic><topic>Cell adhesion & migration</topic><topic>Cell death</topic><topic>Cell Movement - drug effects</topic><topic>Cell proliferation</topic><topic>Cell Proliferation - drug effects</topic><topic>Cells, Cultured</topic><topic>Cytotoxicity</topic><topic>Dentistry</topic><topic>Growth factors</topic><topic>Irradiation</topic><topic>Kinases</topic><topic>Lasers</topic><topic>Low-Level Light Therapy</topic><topic>Male</topic><topic>MAP kinase</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Muscle, Smooth, Vascular - pathology</topic><topic>Myocytes, Smooth Muscle - drug effects</topic><topic>Myocytes, Smooth Muscle - pathology</topic><topic>Myocytes, Smooth Muscle - radiation effects</topic><topic>Optical Devices</topic><topic>Optics</topic><topic>Original Article</topic><topic>Photonics</topic><topic>Platelet-derived growth factor</topic><topic>Platelet-derived growth factor BB</topic><topic>Protein kinase</topic><topic>Proto-Oncogene Proteins c-sis - pharmacology</topic><topic>Quantum Optics</topic><topic>Rats, Sprague-Dawley</topic><topic>Restenosis</topic><topic>Smooth muscle</topic><topic>Toxicity testing</topic><topic>Viability</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Baek, Suji</creatorcontrib><creatorcontrib>Lee, Kang Pa</creatorcontrib><creatorcontrib>Cui, Long</creatorcontrib><creatorcontrib>Ryu, Yunkyoung</creatorcontrib><creatorcontrib>Hong, Jung Min</creatorcontrib><creatorcontrib>Kim, Junghwan</creatorcontrib><creatorcontrib>Jung, Seung Hyo</creatorcontrib><creatorcontrib>Bae, Young Min</creatorcontrib><creatorcontrib>Won, Kyung Jong</creatorcontrib><creatorcontrib>Kim, Bokyung</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Electronics & Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Aerospace Database</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Lasers in medical science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Baek, Suji</au><au>Lee, Kang Pa</au><au>Cui, Long</au><au>Ryu, Yunkyoung</au><au>Hong, Jung Min</au><au>Kim, Junghwan</au><au>Jung, Seung Hyo</au><au>Bae, Young Min</au><au>Won, Kyung Jong</au><au>Kim, Bokyung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Low-power laser irradiation inhibits PDGF-BB-induced migration and proliferation via apoptotic cell death in vascular smooth muscle cells</atitle><jtitle>Lasers in medical science</jtitle><stitle>Lasers Med Sci</stitle><addtitle>Lasers Med Sci</addtitle><date>2017-12-01</date><risdate>2017</risdate><volume>32</volume><issue>9</issue><spage>2121</spage><epage>2127</epage><pages>2121-2127</pages><issn>0268-8921</issn><eissn>1435-604X</eissn><abstract>Vascular restenosis after injury of blood vessel has been implicated in various responses including apoptosis, migration, and proliferation in vascular smooth muscle cells (VSMCs) stimulated by diverse growth factors underlying platelet-derived growth factor (PDGF). Previous studies evaluated the effects of low-power laser (LPL) irradiation over various wavelength ranges on VSMC events in normal and pathologic states. However, whether VSMC responses are affected by LPL irradiation remains unclear. The purpose of this study is to explore the effects of LPL (green diode laser 532-nm pulsed wave of 300 mW at a spot diameter of 1 mm) irradiation on the responses, apoptosis, migration, and proliferation of VSMCs. The effect of LPL irradiation was tested on VSMCs through cytotoxicity, proliferation, migration, and apoptotic assays. Aortic ring assay was used to assess the effect of LPL irradiation on aortic sprout outgrowth. Protein expression levels were determined by western blotting. LPL irradiation did not affect VSMC viability but slightly attenuated PDGF-BB-induced proliferation in VSMCs. In addition, LPL irradiation inhibited PDGF-BB-evoked migration of VSMCs. Aortic sprout outgrowth in response to PDGF-BB was diminished in cells treated with LPL. In contrast, LPL irradiation evoked apoptosis in VSMCs in the presence of PDGF-BB. Similarly, activation of caspase-3 and Bax, as well as p38 mitogen-activated protein kinase (MAPK), in VSMCs treated with PDGF-BB was enhanced by exposure to LPL. These findings indicate that LPL irradiation induces vascular apoptosis via p38 MAPK activation and simultaneously inhibits VSMC proliferation and migration in response to PDGF-BB.</abstract><cop>London</cop><pub>Springer London</pub><pmid>28983687</pmid><doi>10.1007/s10103-017-2338-z</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0268-8921 |
| ispartof | Lasers in medical science, 2017-12, Vol.32 (9), p.2121-2127 |
| issn | 0268-8921 1435-604X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1948757430 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Activation Animals Aorta Aorta - cytology Apoptosis Apoptosis - drug effects BAX protein Blood vessels Caspase Caspase-3 Cell adhesion & migration Cell death Cell Movement - drug effects Cell proliferation Cell Proliferation - drug effects Cells, Cultured Cytotoxicity Dentistry Growth factors Irradiation Kinases Lasers Low-Level Light Therapy Male MAP kinase Medicine Medicine & Public Health Muscle, Smooth, Vascular - pathology Myocytes, Smooth Muscle - drug effects Myocytes, Smooth Muscle - pathology Myocytes, Smooth Muscle - radiation effects Optical Devices Optics Original Article Photonics Platelet-derived growth factor Platelet-derived growth factor BB Protein kinase Proto-Oncogene Proteins c-sis - pharmacology Quantum Optics Rats, Sprague-Dawley Restenosis Smooth muscle Toxicity testing Viability Western blotting |
| title | Low-power laser irradiation inhibits PDGF-BB-induced migration and proliferation via apoptotic cell death in vascular smooth muscle cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T12%3A34%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Low-power%20laser%20irradiation%20inhibits%20PDGF-BB-induced%20migration%20and%20proliferation%20via%20apoptotic%20cell%20death%20in%20vascular%20smooth%20muscle%20cells&rft.jtitle=Lasers%20in%20medical%20science&rft.au=Baek,%20Suji&rft.date=2017-12-01&rft.volume=32&rft.issue=9&rft.spage=2121&rft.epage=2127&rft.pages=2121-2127&rft.issn=0268-8921&rft.eissn=1435-604X&rft_id=info:doi/10.1007/s10103-017-2338-z&rft_dat=%3Cproquest_cross%3E1948757430%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1961740804&rft_id=info:pmid/28983687&rfr_iscdi=true |