Mechanism of Phospholipid Hydrolysis for Oyster Crassostrea plicatula Phospholipids During Storage Using Shotgun Lipidomics
A fast and efficient shotgun lipidomics strategy was applied to analyze phospholipids (PL) in the oyster Crassostrea plicatula , including 29 species of phosphatidylcholine (PtdCho), 23 species of phosphatidylethanolamine (PtdEtn), 11 species of phosphatidylserine (PtdSer), 6 species of phosphatidyl...
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| Veröffentlicht in: | Lipids 2017-12, Vol.52 (12), p.1045-1058 |
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| creator | Chen, Qinsheng Wang, Xincen Cong, Peixu Liu, Yanjun Wang, Yuming Xu, Jie Xue, Changhu |
| description | A fast and efficient shotgun lipidomics strategy was applied to analyze phospholipids (PL) in the oyster
Crassostrea plicatula
, including 29 species of phosphatidylcholine (PtdCho), 23 species of phosphatidylethanolamine (PtdEtn), 11 species of phosphatidylserine (PtdSer), 6 species of phosphatidylinositol (PtdIns), and 17 species of lysophospholipids (Lyso-PL). During storage at 4 °C for 7 days, the PL content decreased by 68.08%, but a significant increase in the FFA content was observed (from 63.11 to 318.72 μg/g). PtdCho and PtdIns decreased relatively by 64.97 and 67.49%, and PtdSer decreased most markedly by 74.15%. However, the PtdEtn content increased slightly during the early stages of storage but subsequently began to decrease. Moreover, PL with eicosapentaenoic acid (EPA-PL) and docosahexaenoic acid (DHA-PL) decreased by 51.77 and 50.61%, whereas plasmalogens were relatively stable showing only a 25.46% decrease. In particular, through enzyme activity analysis of lipase, phospholipase A
1
(PLA
1
), phospholipase A
2
(PLA
2
), phospholipase C (PLC), and phospholipase D (PLD), it was observed that the activities of all these enzymes increased at the early stage at 4 °C, but their activities were at lower levels when the oysters were stored at −20 °C. During the storage period at 4 °C, correlation analysis suggests that the degradation of PtdCho was mostly correlated to PLA
2
(
p
|
| doi_str_mv | 10.1007/s11745-017-4305-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1947099004</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1947099004</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4865-2da142f69857625b0a19a2271555c9b30af1644bcb9b0b9809802ea0341938973</originalsourceid><addsrcrecordid>eNqFkdFr1TAUxoM43HX6B_giAV98qTtJk6Z5lDt1gzs20D2HtDe9N6Ntak6LFP95UztFByIEwoHf9_Gd8xHyisE7BqDOkTElZAZMZSIHmaknZMOkLDOdg3pKNgBcZIIDOyXPEe_TyISWz8gpL7WSooQN-X7t6qPtPXY0NPT2GHA4htYPfk8v530M7YweaRMivZlxdJFuo0UMOEZn6dD62o5Ta_8SIr2You8P9PMYoj04eoc_p2MYD1NPdwsTOl_jC3LS2Bbdy4f_jNx9_PBle5ntbj5dbd_vslqUhcz43jLBm0KXUhVcVmCZtpyrtKmsdZWDbVghRFVXuoJKl5AedxZywXSeFs3PyNvVd4jh6-RwNJ3H2rWt7V2Y0DAtFGgNIBL65hF6H6bYp3SJKnKhSyFZothK1TEgRteYIfrOxtkwMEszZm3GpGbM0oxZQrx-cJ6qzu1_K35VkQC1At986-b_O5rd1e0FAyGTkq9KHJa7u_hH6H_m-QEx7amS</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1963498451</pqid></control><display><type>article</type><title>Mechanism of Phospholipid Hydrolysis for Oyster Crassostrea plicatula Phospholipids During Storage Using Shotgun Lipidomics</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Chen, Qinsheng ; Wang, Xincen ; Cong, Peixu ; Liu, Yanjun ; Wang, Yuming ; Xu, Jie ; Xue, Changhu</creator><creatorcontrib>Chen, Qinsheng ; Wang, Xincen ; Cong, Peixu ; Liu, Yanjun ; Wang, Yuming ; Xu, Jie ; Xue, Changhu</creatorcontrib><description>A fast and efficient shotgun lipidomics strategy was applied to analyze phospholipids (PL) in the oyster
Crassostrea plicatula
, including 29 species of phosphatidylcholine (PtdCho), 23 species of phosphatidylethanolamine (PtdEtn), 11 species of phosphatidylserine (PtdSer), 6 species of phosphatidylinositol (PtdIns), and 17 species of lysophospholipids (Lyso-PL). During storage at 4 °C for 7 days, the PL content decreased by 68.08%, but a significant increase in the FFA content was observed (from 63.11 to 318.72 μg/g). PtdCho and PtdIns decreased relatively by 64.97 and 67.49%, and PtdSer decreased most markedly by 74.15%. However, the PtdEtn content increased slightly during the early stages of storage but subsequently began to decrease. Moreover, PL with eicosapentaenoic acid (EPA-PL) and docosahexaenoic acid (DHA-PL) decreased by 51.77 and 50.61%, whereas plasmalogens were relatively stable showing only a 25.46% decrease. In particular, through enzyme activity analysis of lipase, phospholipase A
1
(PLA
1
), phospholipase A
2
(PLA
2
), phospholipase C (PLC), and phospholipase D (PLD), it was observed that the activities of all these enzymes increased at the early stage at 4 °C, but their activities were at lower levels when the oysters were stored at −20 °C. During the storage period at 4 °C, correlation analysis suggests that the degradation of PtdCho was mostly correlated to PLA
2
(
p
< 0.05), whereas PtdEtn and PtdSer were more markedly correlated to lipase and PLD, respectively. The above result indicates that the hydrolysis mechanism of PL during seafood storage was correlated to the lipid hydrolytic enzyme activities under different storage temperatures.</description><identifier>ISSN: 0024-4201</identifier><identifier>EISSN: 1558-9307</identifier><identifier>DOI: 10.1007/s11745-017-4305-7</identifier><identifier>PMID: 28975480</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Animals ; Biomedical and Life Sciences ; Correlation analysis ; Crassostrea ; Crassostrea - metabolism ; Crassostrea - physiology ; Crassostrea plicatula ; Docosahexaenoic acid ; Eicosapentaenoic acid ; Enzymatic activity ; Enzyme activity ; Enzymes ; Hydrolysis ; Hydrolytic enzyme ; Lecithin ; Life Sciences ; Lipase ; Lipidology ; Lysophospholipids - analysis ; Lysophospholipids - chemistry ; Medical Biochemistry ; Medicinal Chemistry ; Microbial Genetics and Genomics ; Neurochemistry ; Nutrition ; Original Article ; Oysters ; Phosphatidylcholine ; Phosphatidylethanolamine ; Phosphatidylethanolamines - analysis ; Phosphatidylethanolamines - chemistry ; Phosphatidylinositol ; Phosphatidylinositols - analysis ; Phosphatidylinositols - chemistry ; Phosphatidylserine ; Phosphatidylserines - analysis ; Phosphatidylserines - chemistry ; Phospholipase ; Phospholipase A1 ; Phospholipase A2 ; Phospholipase C ; Phospholipase D ; Phospholipids ; Phospholipids - analysis ; Phospholipids - chemistry ; Seafood ; Shotgun lipidomics ; Species ; Storage</subject><ispartof>Lipids, 2017-12, Vol.52 (12), p.1045-1058</ispartof><rights>AOCS 2017</rights><rights>2017 American Oil Chemists' Society (AOCS)</rights><rights>Lipids is a copyright of Springer, (2017). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4865-2da142f69857625b0a19a2271555c9b30af1644bcb9b0b9809802ea0341938973</citedby><cites>FETCH-LOGICAL-c4865-2da142f69857625b0a19a2271555c9b30af1644bcb9b0b9809802ea0341938973</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11745-017-4305-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11745-017-4305-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,41464,42533,45550,45551,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28975480$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Qinsheng</creatorcontrib><creatorcontrib>Wang, Xincen</creatorcontrib><creatorcontrib>Cong, Peixu</creatorcontrib><creatorcontrib>Liu, Yanjun</creatorcontrib><creatorcontrib>Wang, Yuming</creatorcontrib><creatorcontrib>Xu, Jie</creatorcontrib><creatorcontrib>Xue, Changhu</creatorcontrib><title>Mechanism of Phospholipid Hydrolysis for Oyster Crassostrea plicatula Phospholipids During Storage Using Shotgun Lipidomics</title><title>Lipids</title><addtitle>Lipids</addtitle><addtitle>Lipids</addtitle><description>A fast and efficient shotgun lipidomics strategy was applied to analyze phospholipids (PL) in the oyster
Crassostrea plicatula
, including 29 species of phosphatidylcholine (PtdCho), 23 species of phosphatidylethanolamine (PtdEtn), 11 species of phosphatidylserine (PtdSer), 6 species of phosphatidylinositol (PtdIns), and 17 species of lysophospholipids (Lyso-PL). During storage at 4 °C for 7 days, the PL content decreased by 68.08%, but a significant increase in the FFA content was observed (from 63.11 to 318.72 μg/g). PtdCho and PtdIns decreased relatively by 64.97 and 67.49%, and PtdSer decreased most markedly by 74.15%. However, the PtdEtn content increased slightly during the early stages of storage but subsequently began to decrease. Moreover, PL with eicosapentaenoic acid (EPA-PL) and docosahexaenoic acid (DHA-PL) decreased by 51.77 and 50.61%, whereas plasmalogens were relatively stable showing only a 25.46% decrease. In particular, through enzyme activity analysis of lipase, phospholipase A
1
(PLA
1
), phospholipase A
2
(PLA
2
), phospholipase C (PLC), and phospholipase D (PLD), it was observed that the activities of all these enzymes increased at the early stage at 4 °C, but their activities were at lower levels when the oysters were stored at −20 °C. During the storage period at 4 °C, correlation analysis suggests that the degradation of PtdCho was mostly correlated to PLA
2
(
p
< 0.05), whereas PtdEtn and PtdSer were more markedly correlated to lipase and PLD, respectively. The above result indicates that the hydrolysis mechanism of PL during seafood storage was correlated to the lipid hydrolytic enzyme activities under different storage temperatures.</description><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Correlation analysis</subject><subject>Crassostrea</subject><subject>Crassostrea - metabolism</subject><subject>Crassostrea - physiology</subject><subject>Crassostrea plicatula</subject><subject>Docosahexaenoic acid</subject><subject>Eicosapentaenoic acid</subject><subject>Enzymatic activity</subject><subject>Enzyme activity</subject><subject>Enzymes</subject><subject>Hydrolysis</subject><subject>Hydrolytic enzyme</subject><subject>Lecithin</subject><subject>Life Sciences</subject><subject>Lipase</subject><subject>Lipidology</subject><subject>Lysophospholipids - analysis</subject><subject>Lysophospholipids - chemistry</subject><subject>Medical Biochemistry</subject><subject>Medicinal Chemistry</subject><subject>Microbial Genetics and Genomics</subject><subject>Neurochemistry</subject><subject>Nutrition</subject><subject>Original Article</subject><subject>Oysters</subject><subject>Phosphatidylcholine</subject><subject>Phosphatidylethanolamine</subject><subject>Phosphatidylethanolamines - analysis</subject><subject>Phosphatidylethanolamines - chemistry</subject><subject>Phosphatidylinositol</subject><subject>Phosphatidylinositols - analysis</subject><subject>Phosphatidylinositols - chemistry</subject><subject>Phosphatidylserine</subject><subject>Phosphatidylserines - analysis</subject><subject>Phosphatidylserines - chemistry</subject><subject>Phospholipase</subject><subject>Phospholipase A1</subject><subject>Phospholipase A2</subject><subject>Phospholipase C</subject><subject>Phospholipase D</subject><subject>Phospholipids</subject><subject>Phospholipids - analysis</subject><subject>Phospholipids - chemistry</subject><subject>Seafood</subject><subject>Shotgun lipidomics</subject><subject>Species</subject><subject>Storage</subject><issn>0024-4201</issn><issn>1558-9307</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkdFr1TAUxoM43HX6B_giAV98qTtJk6Z5lDt1gzs20D2HtDe9N6Ntak6LFP95UztFByIEwoHf9_Gd8xHyisE7BqDOkTElZAZMZSIHmaknZMOkLDOdg3pKNgBcZIIDOyXPEe_TyISWz8gpL7WSooQN-X7t6qPtPXY0NPT2GHA4htYPfk8v530M7YweaRMivZlxdJFuo0UMOEZn6dD62o5Ta_8SIr2You8P9PMYoj04eoc_p2MYD1NPdwsTOl_jC3LS2Bbdy4f_jNx9_PBle5ntbj5dbd_vslqUhcz43jLBm0KXUhVcVmCZtpyrtKmsdZWDbVghRFVXuoJKl5AedxZywXSeFs3PyNvVd4jh6-RwNJ3H2rWt7V2Y0DAtFGgNIBL65hF6H6bYp3SJKnKhSyFZothK1TEgRteYIfrOxtkwMEszZm3GpGbM0oxZQrx-cJ6qzu1_K35VkQC1At986-b_O5rd1e0FAyGTkq9KHJa7u_hH6H_m-QEx7amS</recordid><startdate>201712</startdate><enddate>201712</enddate><creator>Chen, Qinsheng</creator><creator>Wang, Xincen</creator><creator>Cong, Peixu</creator><creator>Liu, Yanjun</creator><creator>Wang, Yuming</creator><creator>Xu, Jie</creator><creator>Xue, Changhu</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PCBAR</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope></search><sort><creationdate>201712</creationdate><title>Mechanism of Phospholipid Hydrolysis for Oyster Crassostrea plicatula Phospholipids During Storage Using Shotgun Lipidomics</title><author>Chen, Qinsheng ; Wang, Xincen ; Cong, Peixu ; Liu, Yanjun ; Wang, Yuming ; Xu, Jie ; Xue, Changhu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4865-2da142f69857625b0a19a2271555c9b30af1644bcb9b0b9809802ea0341938973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Correlation analysis</topic><topic>Crassostrea</topic><topic>Crassostrea - metabolism</topic><topic>Crassostrea - physiology</topic><topic>Crassostrea plicatula</topic><topic>Docosahexaenoic acid</topic><topic>Eicosapentaenoic acid</topic><topic>Enzymatic activity</topic><topic>Enzyme activity</topic><topic>Enzymes</topic><topic>Hydrolysis</topic><topic>Hydrolytic enzyme</topic><topic>Lecithin</topic><topic>Life Sciences</topic><topic>Lipase</topic><topic>Lipidology</topic><topic>Lysophospholipids - analysis</topic><topic>Lysophospholipids - chemistry</topic><topic>Medical Biochemistry</topic><topic>Medicinal Chemistry</topic><topic>Microbial Genetics and Genomics</topic><topic>Neurochemistry</topic><topic>Nutrition</topic><topic>Original Article</topic><topic>Oysters</topic><topic>Phosphatidylcholine</topic><topic>Phosphatidylethanolamine</topic><topic>Phosphatidylethanolamines - analysis</topic><topic>Phosphatidylethanolamines - chemistry</topic><topic>Phosphatidylinositol</topic><topic>Phosphatidylinositols - analysis</topic><topic>Phosphatidylinositols - chemistry</topic><topic>Phosphatidylserine</topic><topic>Phosphatidylserines - analysis</topic><topic>Phosphatidylserines - chemistry</topic><topic>Phospholipase</topic><topic>Phospholipase A1</topic><topic>Phospholipase A2</topic><topic>Phospholipase C</topic><topic>Phospholipase D</topic><topic>Phospholipids</topic><topic>Phospholipids - analysis</topic><topic>Phospholipids - chemistry</topic><topic>Seafood</topic><topic>Shotgun lipidomics</topic><topic>Species</topic><topic>Storage</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Qinsheng</creatorcontrib><creatorcontrib>Wang, Xincen</creatorcontrib><creatorcontrib>Cong, Peixu</creatorcontrib><creatorcontrib>Liu, Yanjun</creatorcontrib><creatorcontrib>Wang, Yuming</creatorcontrib><creatorcontrib>Xu, Jie</creatorcontrib><creatorcontrib>Xue, Changhu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>ProQuest Health & Medical Research Collection</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Health & Nursing</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Applied & Life Sciences</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>Lipids</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Qinsheng</au><au>Wang, Xincen</au><au>Cong, Peixu</au><au>Liu, Yanjun</au><au>Wang, Yuming</au><au>Xu, Jie</au><au>Xue, Changhu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mechanism of Phospholipid Hydrolysis for Oyster Crassostrea plicatula Phospholipids During Storage Using Shotgun Lipidomics</atitle><jtitle>Lipids</jtitle><stitle>Lipids</stitle><addtitle>Lipids</addtitle><date>2017-12</date><risdate>2017</risdate><volume>52</volume><issue>12</issue><spage>1045</spage><epage>1058</epage><pages>1045-1058</pages><issn>0024-4201</issn><eissn>1558-9307</eissn><abstract>A fast and efficient shotgun lipidomics strategy was applied to analyze phospholipids (PL) in the oyster
Crassostrea plicatula
, including 29 species of phosphatidylcholine (PtdCho), 23 species of phosphatidylethanolamine (PtdEtn), 11 species of phosphatidylserine (PtdSer), 6 species of phosphatidylinositol (PtdIns), and 17 species of lysophospholipids (Lyso-PL). During storage at 4 °C for 7 days, the PL content decreased by 68.08%, but a significant increase in the FFA content was observed (from 63.11 to 318.72 μg/g). PtdCho and PtdIns decreased relatively by 64.97 and 67.49%, and PtdSer decreased most markedly by 74.15%. However, the PtdEtn content increased slightly during the early stages of storage but subsequently began to decrease. Moreover, PL with eicosapentaenoic acid (EPA-PL) and docosahexaenoic acid (DHA-PL) decreased by 51.77 and 50.61%, whereas plasmalogens were relatively stable showing only a 25.46% decrease. In particular, through enzyme activity analysis of lipase, phospholipase A
1
(PLA
1
), phospholipase A
2
(PLA
2
), phospholipase C (PLC), and phospholipase D (PLD), it was observed that the activities of all these enzymes increased at the early stage at 4 °C, but their activities were at lower levels when the oysters were stored at −20 °C. During the storage period at 4 °C, correlation analysis suggests that the degradation of PtdCho was mostly correlated to PLA
2
(
p
< 0.05), whereas PtdEtn and PtdSer were more markedly correlated to lipase and PLD, respectively. The above result indicates that the hydrolysis mechanism of PL during seafood storage was correlated to the lipid hydrolytic enzyme activities under different storage temperatures.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>28975480</pmid><doi>10.1007/s11745-017-4305-7</doi><tpages>14</tpages></addata></record> |
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| ispartof | Lipids, 2017-12, Vol.52 (12), p.1045-1058 |
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| subjects | Animals Biomedical and Life Sciences Correlation analysis Crassostrea Crassostrea - metabolism Crassostrea - physiology Crassostrea plicatula Docosahexaenoic acid Eicosapentaenoic acid Enzymatic activity Enzyme activity Enzymes Hydrolysis Hydrolytic enzyme Lecithin Life Sciences Lipase Lipidology Lysophospholipids - analysis Lysophospholipids - chemistry Medical Biochemistry Medicinal Chemistry Microbial Genetics and Genomics Neurochemistry Nutrition Original Article Oysters Phosphatidylcholine Phosphatidylethanolamine Phosphatidylethanolamines - analysis Phosphatidylethanolamines - chemistry Phosphatidylinositol Phosphatidylinositols - analysis Phosphatidylinositols - chemistry Phosphatidylserine Phosphatidylserines - analysis Phosphatidylserines - chemistry Phospholipase Phospholipase A1 Phospholipase A2 Phospholipase C Phospholipase D Phospholipids Phospholipids - analysis Phospholipids - chemistry Seafood Shotgun lipidomics Species Storage |
| title | Mechanism of Phospholipid Hydrolysis for Oyster Crassostrea plicatula Phospholipids During Storage Using Shotgun Lipidomics |
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