Coupled transcript and metabolite identification: insights on induction and synthesis of resveratrol in peanut, wild relatives and synthetic allotetraploid
Resveratrol is an antioxidant that is a promising antitumoral, cardioprotective and neuroprotective agent. It has been found in a restricted number of plants including peanut (Arachis hypogaea L.) and its wild relatives. The objective of this study was to understand the relationship between resverat...
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| Veröffentlicht in: | Genetics and molecular research 2017-09, Vol.16 (3), p.1 |
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| creator | Carvalho, P A S V Brasileiro, A C Leal-Bertioli, S Bertioli, D J Silva, J P Agostini-Costa, T S Gimenes, M A |
| description | Resveratrol is an antioxidant that is a promising antitumoral, cardioprotective and neuroprotective agent. It has been found in a restricted number of plants including peanut (Arachis hypogaea L.) and its wild relatives. The objective of this study was to understand the relationship between resveratrol content and the expression of putative resveratrol synthase genes in four Arachis genotypes. Two diploids and two tetraploid were analyzed. Contents of resveratrol on non- and UV-treated leaves were estimated using HPLC. Resveratrol synthase (RS) was analyzed using RT-qPCR with primers developed in this study. Sequences of six Arachis species were amplified using two degenerated primer pairs that were designed based on Arachis and general RS available at GenBank. Those sequences were used to qPCR primers design. Test and control leaves were collected from plants cultivated in greenhouse and three biological replicates were evaluated for each genotype. The synthesis of resveratrol in leaves was induced by treatment with UV for 2.5 h. All genotypes studied synthesized resveratrol. Concentrations ranged from 193.66 µg/g in synthetic allotetraploid to 371.97 µg/g in A. duranensis. Natural and induced allotetraoploids showed lower levels of resveratrol than their diploid parents. Untreated samples did not produce significant amounts of resveratrol. The analysis of resveratrol content and levels of RS mRNA allowed the identification of one gene induced by the UV treatment. The data showed different amounts of RS in the different genotypes suggesting early and late response to the UV induction in the different species. The understanding of the variation found among species will help to identify species that have high resveratrol content and their ideal pos-induction times. This also will allow analysis of other tissues where high levels resveratrol would be very important, such as in seeds. |
| doi_str_mv | 10.4238/gmr16039802 |
| format | Article |
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It has been found in a restricted number of plants including peanut (Arachis hypogaea L.) and its wild relatives. The objective of this study was to understand the relationship between resveratrol content and the expression of putative resveratrol synthase genes in four Arachis genotypes. Two diploids and two tetraploid were analyzed. Contents of resveratrol on non- and UV-treated leaves were estimated using HPLC. Resveratrol synthase (RS) was analyzed using RT-qPCR with primers developed in this study. Sequences of six Arachis species were amplified using two degenerated primer pairs that were designed based on Arachis and general RS available at GenBank. Those sequences were used to qPCR primers design. Test and control leaves were collected from plants cultivated in greenhouse and three biological replicates were evaluated for each genotype. The synthesis of resveratrol in leaves was induced by treatment with UV for 2.5 h. All genotypes studied synthesized resveratrol. Concentrations ranged from 193.66 µg/g in synthetic allotetraploid to 371.97 µg/g in A. duranensis. Natural and induced allotetraoploids showed lower levels of resveratrol than their diploid parents. Untreated samples did not produce significant amounts of resveratrol. The analysis of resveratrol content and levels of RS mRNA allowed the identification of one gene induced by the UV treatment. The data showed different amounts of RS in the different genotypes suggesting early and late response to the UV induction in the different species. The understanding of the variation found among species will help to identify species that have high resveratrol content and their ideal pos-induction times. This also will allow analysis of other tissues where high levels resveratrol would be very important, such as in seeds.</description><identifier>EISSN: 1676-5680</identifier><identifier>DOI: 10.4238/gmr16039802</identifier><identifier>PMID: 28973779</identifier><language>eng</language><publisher>Brazil: Fundacao de Pesquisas Cientificas de Ribeirao Preto</publisher><subject>Antioxidants ; Arachis ; Arachis - genetics ; Arachis - metabolism ; Diploids ; Genotype ; Genotypes ; High-performance liquid chromatography ; Leaves ; Neuroprotection ; Plant Leaves - genetics ; Plant Leaves - metabolism ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Primers ; Resveratrol ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Seeds ; Species ; Stilbenes - metabolism ; Tetraploidy ; Transcription ; Transcriptional Activation - radiation effects ; Ultraviolet radiation ; Ultraviolet Rays</subject><ispartof>Genetics and molecular research, 2017-09, Vol.16 (3), p.1</ispartof><rights>Copyright Fundacao de Pesquisas Cientificas de Ribeirao Preto 2017</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28973779$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Carvalho, P A S V</creatorcontrib><creatorcontrib>Brasileiro, A C</creatorcontrib><creatorcontrib>Leal-Bertioli, S</creatorcontrib><creatorcontrib>Bertioli, D J</creatorcontrib><creatorcontrib>Silva, J P</creatorcontrib><creatorcontrib>Agostini-Costa, T S</creatorcontrib><creatorcontrib>Gimenes, M A</creatorcontrib><title>Coupled transcript and metabolite identification: insights on induction and synthesis of resveratrol in peanut, wild relatives and synthetic allotetraploid</title><title>Genetics and molecular research</title><addtitle>Genet Mol Res</addtitle><description>Resveratrol is an antioxidant that is a promising antitumoral, cardioprotective and neuroprotective agent. It has been found in a restricted number of plants including peanut (Arachis hypogaea L.) and its wild relatives. The objective of this study was to understand the relationship between resveratrol content and the expression of putative resveratrol synthase genes in four Arachis genotypes. Two diploids and two tetraploid were analyzed. Contents of resveratrol on non- and UV-treated leaves were estimated using HPLC. Resveratrol synthase (RS) was analyzed using RT-qPCR with primers developed in this study. Sequences of six Arachis species were amplified using two degenerated primer pairs that were designed based on Arachis and general RS available at GenBank. Those sequences were used to qPCR primers design. Test and control leaves were collected from plants cultivated in greenhouse and three biological replicates were evaluated for each genotype. The synthesis of resveratrol in leaves was induced by treatment with UV for 2.5 h. All genotypes studied synthesized resveratrol. Concentrations ranged from 193.66 µg/g in synthetic allotetraploid to 371.97 µg/g in A. duranensis. Natural and induced allotetraoploids showed lower levels of resveratrol than their diploid parents. Untreated samples did not produce significant amounts of resveratrol. The analysis of resveratrol content and levels of RS mRNA allowed the identification of one gene induced by the UV treatment. The data showed different amounts of RS in the different genotypes suggesting early and late response to the UV induction in the different species. The understanding of the variation found among species will help to identify species that have high resveratrol content and their ideal pos-induction times. This also will allow analysis of other tissues where high levels resveratrol would be very important, such as in seeds.</description><subject>Antioxidants</subject><subject>Arachis</subject><subject>Arachis - genetics</subject><subject>Arachis - metabolism</subject><subject>Diploids</subject><subject>Genotype</subject><subject>Genotypes</subject><subject>High-performance liquid chromatography</subject><subject>Leaves</subject><subject>Neuroprotection</subject><subject>Plant Leaves - genetics</subject><subject>Plant Leaves - metabolism</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Primers</subject><subject>Resveratrol</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Seeds</subject><subject>Species</subject><subject>Stilbenes - metabolism</subject><subject>Tetraploidy</subject><subject>Transcription</subject><subject>Transcriptional Activation - radiation effects</subject><subject>Ultraviolet radiation</subject><subject>Ultraviolet Rays</subject><issn>1676-5680</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkE9LJDEQxYOwODp68i6BvexhZ00n6U7iTYb9B8Je9Dykk8oYSSe9SVrxs_hljbsuyJ6qqPd7VY9C6KwjXzhl8mI_5W4gTElCD9BRN4hh0w-SrNBxKfeE0J5LcohWVCrBhFBH6HmbljmAxTXrWEz2c8U6WjxB1WMKvgL2FmL1zhtdfYqX2Mfi93e14BRbbxfzOv5jKk-x3kHxTXI4Q3mArGtOoWF4Bh2X-hk_-mCbFtqyByjvbNUbrENIFVqUOSRvT9AHp0OB07e6Rrffvt5sf2yuf33_ub263syUqbqx4IwcAUhvoTdCai70wKnkWjo7GMppP1LGmBmVEMI5Lt3QO6GUa6gZKFujT3_3zjn9XqDU3eSLgRB0hLSUXae4IEr2HW_ox__Q-7Tk2NI1SjGpVNcurdH5G7WME9jdnP2k89Pu39vZC1Vfhq4</recordid><startdate>20170927</startdate><enddate>20170927</enddate><creator>Carvalho, P A S V</creator><creator>Brasileiro, A C</creator><creator>Leal-Bertioli, S</creator><creator>Bertioli, D J</creator><creator>Silva, J P</creator><creator>Agostini-Costa, T S</creator><creator>Gimenes, M A</creator><general>Fundacao de Pesquisas Cientificas de Ribeirao Preto</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QP</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20170927</creationdate><title>Coupled transcript and metabolite identification: insights on induction and synthesis of resveratrol in peanut, wild relatives and synthetic allotetraploid</title><author>Carvalho, P A S V ; Brasileiro, A C ; Leal-Bertioli, S ; Bertioli, D J ; Silva, J P ; Agostini-Costa, T S ; Gimenes, M A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p239t-defc8bee05de5c78a47a64284a8fd6c2425b2333cb9777ff48f65f799f8a4c623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Antioxidants</topic><topic>Arachis</topic><topic>Arachis - genetics</topic><topic>Arachis - metabolism</topic><topic>Diploids</topic><topic>Genotype</topic><topic>Genotypes</topic><topic>High-performance liquid chromatography</topic><topic>Leaves</topic><topic>Neuroprotection</topic><topic>Plant Leaves - genetics</topic><topic>Plant Leaves - metabolism</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Primers</topic><topic>Resveratrol</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Seeds</topic><topic>Species</topic><topic>Stilbenes - metabolism</topic><topic>Tetraploidy</topic><topic>Transcription</topic><topic>Transcriptional Activation - radiation effects</topic><topic>Ultraviolet radiation</topic><topic>Ultraviolet Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Carvalho, P A S V</creatorcontrib><creatorcontrib>Brasileiro, A C</creatorcontrib><creatorcontrib>Leal-Bertioli, S</creatorcontrib><creatorcontrib>Bertioli, D J</creatorcontrib><creatorcontrib>Silva, J P</creatorcontrib><creatorcontrib>Agostini-Costa, T S</creatorcontrib><creatorcontrib>Gimenes, M A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Genetics and molecular research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Carvalho, P A S V</au><au>Brasileiro, A C</au><au>Leal-Bertioli, S</au><au>Bertioli, D J</au><au>Silva, J P</au><au>Agostini-Costa, T S</au><au>Gimenes, M A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Coupled transcript and metabolite identification: insights on induction and synthesis of resveratrol in peanut, wild relatives and synthetic allotetraploid</atitle><jtitle>Genetics and molecular research</jtitle><addtitle>Genet Mol Res</addtitle><date>2017-09-27</date><risdate>2017</risdate><volume>16</volume><issue>3</issue><spage>1</spage><pages>1-</pages><eissn>1676-5680</eissn><abstract>Resveratrol is an antioxidant that is a promising antitumoral, cardioprotective and neuroprotective agent. It has been found in a restricted number of plants including peanut (Arachis hypogaea L.) and its wild relatives. The objective of this study was to understand the relationship between resveratrol content and the expression of putative resveratrol synthase genes in four Arachis genotypes. Two diploids and two tetraploid were analyzed. Contents of resveratrol on non- and UV-treated leaves were estimated using HPLC. Resveratrol synthase (RS) was analyzed using RT-qPCR with primers developed in this study. Sequences of six Arachis species were amplified using two degenerated primer pairs that were designed based on Arachis and general RS available at GenBank. Those sequences were used to qPCR primers design. Test and control leaves were collected from plants cultivated in greenhouse and three biological replicates were evaluated for each genotype. The synthesis of resveratrol in leaves was induced by treatment with UV for 2.5 h. All genotypes studied synthesized resveratrol. Concentrations ranged from 193.66 µg/g in synthetic allotetraploid to 371.97 µg/g in A. duranensis. Natural and induced allotetraoploids showed lower levels of resveratrol than their diploid parents. Untreated samples did not produce significant amounts of resveratrol. The analysis of resveratrol content and levels of RS mRNA allowed the identification of one gene induced by the UV treatment. The data showed different amounts of RS in the different genotypes suggesting early and late response to the UV induction in the different species. The understanding of the variation found among species will help to identify species that have high resveratrol content and their ideal pos-induction times. This also will allow analysis of other tissues where high levels resveratrol would be very important, such as in seeds.</abstract><cop>Brazil</cop><pub>Fundacao de Pesquisas Cientificas de Ribeirao Preto</pub><pmid>28973779</pmid><doi>10.4238/gmr16039802</doi></addata></record> |
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| identifier | EISSN: 1676-5680 |
| ispartof | Genetics and molecular research, 2017-09, Vol.16 (3), p.1 |
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| language | eng |
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| subjects | Antioxidants Arachis Arachis - genetics Arachis - metabolism Diploids Genotype Genotypes High-performance liquid chromatography Leaves Neuroprotection Plant Leaves - genetics Plant Leaves - metabolism Plant Proteins - genetics Plant Proteins - metabolism Primers Resveratrol RNA, Messenger - genetics RNA, Messenger - metabolism Seeds Species Stilbenes - metabolism Tetraploidy Transcription Transcriptional Activation - radiation effects Ultraviolet radiation Ultraviolet Rays |
| title | Coupled transcript and metabolite identification: insights on induction and synthesis of resveratrol in peanut, wild relatives and synthetic allotetraploid |
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