Characterization of an experimental miniature bioreactor for cellular perturbation studies
A mini bioreactor (3.0 mL volume) has been developed and shown to be a versatile tool for rapidly screening and quantifying the response of organisms on environmental perturbations. The mini bioreactor is essentially a plug flow device transformed into a well‐mixed reactor by a recycle flow of the b...
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| Veröffentlicht in: | Biotechnology and bioengineering 2006-12, Vol.95 (6), p.1032-1042 |
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| container_title | Biotechnology and bioengineering |
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| creator | Aboka, Fredrick O. Yang, Huiling de Jonge, Lodewijk P. Kerste, Rob van Winden, Wouter A. van Gulik, Walter M. Hoogendijk, Rob Oudshoorn, Arthur Heijnen, Joseph J. |
| description | A mini bioreactor (3.0 mL volume) has been developed and shown to be a versatile tool for rapidly screening and quantifying the response of organisms on environmental perturbations. The mini bioreactor is essentially a plug flow device transformed into a well‐mixed reactor by a recycle flow of the broth. The gas and liquid phases are separated by a silicone membrane. Dynamic mass transfer experiments were performed to determine the mass transfer capacities for oxygen and carbon dioxide. The mass transfer coefficients for oxygen and carbon dioxide were found to be 1.55 ± 0.17 × 10−5 m/s and 4.52 ± 0.60 × 10−6 m/s, respectively. Cultivation experiments with the 3.0 mL bioreactor show that (i) it can maintain biomass in the same physiological state as the 4.0 L lab scale bioreactor, (ii) reproducible perturbation experiments such as changing substrate uptake rate can be readily performed and the physiological response monitored quantitatively in terms of the O2 and CO2 uptake and production rates. © 2006 Wiley Periodicals, Inc. |
| doi_str_mv | 10.1002/bit.21003 |
| format | Article |
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The mini bioreactor is essentially a plug flow device transformed into a well‐mixed reactor by a recycle flow of the broth. The gas and liquid phases are separated by a silicone membrane. Dynamic mass transfer experiments were performed to determine the mass transfer capacities for oxygen and carbon dioxide. The mass transfer coefficients for oxygen and carbon dioxide were found to be 1.55 ± 0.17 × 10−5 m/s and 4.52 ± 0.60 × 10−6 m/s, respectively. Cultivation experiments with the 3.0 mL bioreactor show that (i) it can maintain biomass in the same physiological state as the 4.0 L lab scale bioreactor, (ii) reproducible perturbation experiments such as changing substrate uptake rate can be readily performed and the physiological response monitored quantitatively in terms of the O2 and CO2 uptake and production rates. © 2006 Wiley Periodicals, Inc.</description><identifier>ISSN: 0006-3592</identifier><identifier>EISSN: 1097-0290</identifier><identifier>DOI: 10.1002/bit.21003</identifier><identifier>PMID: 16977621</identifier><identifier>CODEN: BIBIAU</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Biodegradation, Environmental ; Biological and medical sciences ; Biomass ; Biomedical Research ; Bioreactors ; Biotechnology ; Biotechnology - methods ; Carbon dioxide ; Carbon Dioxide - chemistry ; Fermentation ; Fundamental and applied biological sciences. Psychology ; Glucose - metabolism ; Industrial Microbiology - methods ; mass transfer ; Membrane reactors ; mini bioreactor ; Miniaturization ; Models, Statistical ; Oxygen ; Oxygen - chemistry ; perturbation ; quantitative response in O2 and CO2 ; RTD ; Saccharomyces cerevisiae - metabolism ; screening ; Silicones - chemistry ; Studies ; Time Factors</subject><ispartof>Biotechnology and bioengineering, 2006-12, Vol.95 (6), p.1032-1042</ispartof><rights>Copyright © 2006 Wiley Periodicals, Inc.</rights><rights>2007 INIST-CNRS</rights><rights>Copyright 2006 Wiley Periodicals, Inc.</rights><rights>Copyright John Wiley and Sons, Limited Dec 20, 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4903-75f608d9d92abb1d3b78659fc0a0fe67bf69cac6c7c7bcbfdbccc103b83ed4c33</citedby><cites>FETCH-LOGICAL-c4903-75f608d9d92abb1d3b78659fc0a0fe67bf69cac6c7c7bcbfdbccc103b83ed4c33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbit.21003$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbit.21003$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18311680$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16977621$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Aboka, Fredrick O.</creatorcontrib><creatorcontrib>Yang, Huiling</creatorcontrib><creatorcontrib>de Jonge, Lodewijk P.</creatorcontrib><creatorcontrib>Kerste, Rob</creatorcontrib><creatorcontrib>van Winden, Wouter A.</creatorcontrib><creatorcontrib>van Gulik, Walter M.</creatorcontrib><creatorcontrib>Hoogendijk, Rob</creatorcontrib><creatorcontrib>Oudshoorn, Arthur</creatorcontrib><creatorcontrib>Heijnen, Joseph J.</creatorcontrib><title>Characterization of an experimental miniature bioreactor for cellular perturbation studies</title><title>Biotechnology and bioengineering</title><addtitle>Biotechnol. Bioeng</addtitle><description>A mini bioreactor (3.0 mL volume) has been developed and shown to be a versatile tool for rapidly screening and quantifying the response of organisms on environmental perturbations. The mini bioreactor is essentially a plug flow device transformed into a well‐mixed reactor by a recycle flow of the broth. The gas and liquid phases are separated by a silicone membrane. Dynamic mass transfer experiments were performed to determine the mass transfer capacities for oxygen and carbon dioxide. The mass transfer coefficients for oxygen and carbon dioxide were found to be 1.55 ± 0.17 × 10−5 m/s and 4.52 ± 0.60 × 10−6 m/s, respectively. Cultivation experiments with the 3.0 mL bioreactor show that (i) it can maintain biomass in the same physiological state as the 4.0 L lab scale bioreactor, (ii) reproducible perturbation experiments such as changing substrate uptake rate can be readily performed and the physiological response monitored quantitatively in terms of the O2 and CO2 uptake and production rates. © 2006 Wiley Periodicals, Inc.</description><subject>Biodegradation, Environmental</subject><subject>Biological and medical sciences</subject><subject>Biomass</subject><subject>Biomedical Research</subject><subject>Bioreactors</subject><subject>Biotechnology</subject><subject>Biotechnology - methods</subject><subject>Carbon dioxide</subject><subject>Carbon Dioxide - chemistry</subject><subject>Fermentation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucose - metabolism</subject><subject>Industrial Microbiology - methods</subject><subject>mass transfer</subject><subject>Membrane reactors</subject><subject>mini bioreactor</subject><subject>Miniaturization</subject><subject>Models, Statistical</subject><subject>Oxygen</subject><subject>Oxygen - chemistry</subject><subject>perturbation</subject><subject>quantitative response in O2 and CO2</subject><subject>RTD</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>screening</subject><subject>Silicones - chemistry</subject><subject>Studies</subject><subject>Time Factors</subject><issn>0006-3592</issn><issn>1097-0290</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kEtLxDAUhYMoOj4W_gEpgoKLatJ0kmap42MEURc-wE24SROMdtoxafHx683YUUFwEfL67jmHg9AmwfsE4-xAuXY_iye6gAYEC57iTOBFNMAYs5QORbaCVkN4ildeMLaMVggTnLOMDNDD6BE86NZ49wGta-qksQnUiXmbxqeJqVuokomrHbSdN4lyjTcRb3xi49KmqroKfBLh-K96hdB2pTNhHS1ZqILZmO9r6Pb05GY0Ti-uzs5HhxepzgWmKR9ahotSlCIDpUhJVQw5FFZjwNYwriwTGjTTXHOllS2V1ppgqgpqylxTuoZ2e92pb146E1o5cWGWDGrTdEESkTOWMxLB7T_gU9P5OmaTGaGxj4LjCO31kPZNCN5YOY09gH-XBMtZ2zK2Lb_ajuzWXLBTE1P-kvN6I7AzByBoqKyHWrvwyxWUEFbMTA967tVV5v1_R3l0fvNtnfYTLrTm7WcC_LNknPKhvL88k5ciOx7f5WN5TT8Bq6qnUQ</recordid><startdate>20061220</startdate><enddate>20061220</enddate><creator>Aboka, Fredrick O.</creator><creator>Yang, Huiling</creator><creator>de Jonge, Lodewijk P.</creator><creator>Kerste, Rob</creator><creator>van Winden, Wouter A.</creator><creator>van Gulik, Walter M.</creator><creator>Hoogendijk, Rob</creator><creator>Oudshoorn, Arthur</creator><creator>Heijnen, Joseph J.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope></search><sort><creationdate>20061220</creationdate><title>Characterization of an experimental miniature bioreactor for cellular perturbation studies</title><author>Aboka, Fredrick O. ; Yang, Huiling ; de Jonge, Lodewijk P. ; Kerste, Rob ; van Winden, Wouter A. ; van Gulik, Walter M. ; Hoogendijk, Rob ; Oudshoorn, Arthur ; Heijnen, Joseph J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4903-75f608d9d92abb1d3b78659fc0a0fe67bf69cac6c7c7bcbfdbccc103b83ed4c33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Biodegradation, Environmental</topic><topic>Biological and medical sciences</topic><topic>Biomass</topic><topic>Biomedical Research</topic><topic>Bioreactors</topic><topic>Biotechnology</topic><topic>Biotechnology - methods</topic><topic>Carbon dioxide</topic><topic>Carbon Dioxide - chemistry</topic><topic>Fermentation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucose - metabolism</topic><topic>Industrial Microbiology - methods</topic><topic>mass transfer</topic><topic>Membrane reactors</topic><topic>mini bioreactor</topic><topic>Miniaturization</topic><topic>Models, Statistical</topic><topic>Oxygen</topic><topic>Oxygen - chemistry</topic><topic>perturbation</topic><topic>quantitative response in O2 and CO2</topic><topic>RTD</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>screening</topic><topic>Silicones - chemistry</topic><topic>Studies</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aboka, Fredrick O.</creatorcontrib><creatorcontrib>Yang, Huiling</creatorcontrib><creatorcontrib>de Jonge, Lodewijk P.</creatorcontrib><creatorcontrib>Kerste, Rob</creatorcontrib><creatorcontrib>van Winden, Wouter A.</creatorcontrib><creatorcontrib>van Gulik, Walter M.</creatorcontrib><creatorcontrib>Hoogendijk, Rob</creatorcontrib><creatorcontrib>Oudshoorn, Arthur</creatorcontrib><creatorcontrib>Heijnen, Joseph J.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biotechnology and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aboka, Fredrick O.</au><au>Yang, Huiling</au><au>de Jonge, Lodewijk P.</au><au>Kerste, Rob</au><au>van Winden, Wouter A.</au><au>van Gulik, Walter M.</au><au>Hoogendijk, Rob</au><au>Oudshoorn, Arthur</au><au>Heijnen, Joseph J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of an experimental miniature bioreactor for cellular perturbation studies</atitle><jtitle>Biotechnology and bioengineering</jtitle><addtitle>Biotechnol. Bioeng</addtitle><date>2006-12-20</date><risdate>2006</risdate><volume>95</volume><issue>6</issue><spage>1032</spage><epage>1042</epage><pages>1032-1042</pages><issn>0006-3592</issn><eissn>1097-0290</eissn><coden>BIBIAU</coden><abstract>A mini bioreactor (3.0 mL volume) has been developed and shown to be a versatile tool for rapidly screening and quantifying the response of organisms on environmental perturbations. The mini bioreactor is essentially a plug flow device transformed into a well‐mixed reactor by a recycle flow of the broth. The gas and liquid phases are separated by a silicone membrane. Dynamic mass transfer experiments were performed to determine the mass transfer capacities for oxygen and carbon dioxide. The mass transfer coefficients for oxygen and carbon dioxide were found to be 1.55 ± 0.17 × 10−5 m/s and 4.52 ± 0.60 × 10−6 m/s, respectively. Cultivation experiments with the 3.0 mL bioreactor show that (i) it can maintain biomass in the same physiological state as the 4.0 L lab scale bioreactor, (ii) reproducible perturbation experiments such as changing substrate uptake rate can be readily performed and the physiological response monitored quantitatively in terms of the O2 and CO2 uptake and production rates. © 2006 Wiley Periodicals, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>16977621</pmid><doi>10.1002/bit.21003</doi><tpages>11</tpages></addata></record> |
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| subjects | Biodegradation, Environmental Biological and medical sciences Biomass Biomedical Research Bioreactors Biotechnology Biotechnology - methods Carbon dioxide Carbon Dioxide - chemistry Fermentation Fundamental and applied biological sciences. Psychology Glucose - metabolism Industrial Microbiology - methods mass transfer Membrane reactors mini bioreactor Miniaturization Models, Statistical Oxygen Oxygen - chemistry perturbation quantitative response in O2 and CO2 RTD Saccharomyces cerevisiae - metabolism screening Silicones - chemistry Studies Time Factors |
| title | Characterization of an experimental miniature bioreactor for cellular perturbation studies |
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