Delivery of Telomerase Reverse Transcriptase Small Interfering RNA in Complex with Positively Charged Single-Walled Carbon Nanotubes Suppresses Tumor Growth
Purpose: To determine whether -CONH-(CH 2 ) 6 -NH 3 + Cl − functionalized single-walled carbon nanotubes (SWNT) carrying complexed small interfering RNA (siRNA) can enter into tumor cells, wherein they release the siRNA to silence the targeted gene. Experimental Design: -CONH-(CH 2 ) 6 -NH 3 + Cl −...
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| Veröffentlicht in: | Clinical cancer research 2006-08, Vol.12 (16), p.4933-4939 |
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| container_title | Clinical cancer research |
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| creator | Zhang, Zhuohan Yang, Xiaoying Zhang, Yuan Zeng, Bin Wang, Shujing Zhu, Tianhui Roden, Richard B S Chen, Yongsheng Yang, Rongcun |
| description | Purpose: To determine whether -CONH-(CH 2 ) 6 -NH 3 + Cl − functionalized single-walled carbon nanotubes (SWNT) carrying complexed small interfering RNA (siRNA) can enter into tumor
cells, wherein they release the siRNA to silence the targeted gene.
Experimental Design: -CONH-(CH 2 ) 6 -NH 3 + Cl − was used to mediate the conjugation of telomerase reverse transcriptase (TERT) siRNA to SWNTs. The ability of TERT siRNA
delivered via SWNT complexes to silence the expression of TERT was assessed by their effects on the proliferation and growth
of tumor cells both in vitro and in mouse models.
Results: The functionalized SWNTs -CONH-(CH 2 ) 6 -NH 3 + Cl − could facilitate the coupling of siRNAs that specifically target murine TERT expression to form the mTERT siRNA:SWNT+ complex.
These functionalized SWNTs rapidly entered three cultured murine tumor cell lines, suppressed mTERT expression, and produced
growth arrest. Injection of mTERT siRNA:SWNT+ complexes into s.c. Lewis lung tumors reduced tumor growth. Furthermore, human
TERT siRNA:SWNT+ complexes also suppressed the growth of human HeLa cells both in vitro and when injected into tumors in nude mice.
Conclusions: -CONH-(CH 2 ) 6 -NH 3 + Cl − functionalized SWNTs carry complexed siRNA into tumor cells, wherein they release the siRNA from the nanotube sidewalls to
silence the targeted gene. The -CONH-(CH 2 ) 6 -NH 3 + Cl − functionalized SWNTs may represent a new class of molecular transporters applicable for siRNA therapeutics. |
| doi_str_mv | 10.1158/1078-0432.CCR-05-2831 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_19465366</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>19465366</sourcerecordid><originalsourceid>FETCH-LOGICAL-c501t-14436c65857c124f9577141abd1ca8ed5cb9f5029a233ea31633247a1a059cb33</originalsourceid><addsrcrecordid>eNpFkd1u1DAQhSMEoqXwCCDfgMRFin_zc1kFKJWqgnYXcWk53snGyInDOGHZd-Fh8WoX9WrGo-_MyOdk2WtGrxlT1QdGyyqnUvDrplnlVOW8EuxJdsmUKnPBC_U09f-Zi-xFjD8pZZJR-Ty7YEXNpKr4Zfb3I3j3G_BAQkc24MMAaCKQFaRhqhs0Y7Topvk4XQ_Ge3I3zoAdoBt3ZPVwQ9xImjBMHv6QvZt78i1EN6el_kCa3uAOtmSdWA_5jyRPr8ZgG0byYMYwLy1Esl6mCSHG1G6WISC5xbCf-5fZs874CK_O9Sr7_vnTpvmS33-9vWtu7nOrKJtzJqUobKEqVVrGZVerskw_Ne2WWVPBVtm27hTlteFCgBGsEILL0jBDVW1bIa6yd6e9E4ZfC8RZDy5a8N6MEJaoWS0LJYoigeoEWgwxInR6QjcYPGhG9TEWfbRcHy3XKRZNlT7GknRvzgeWdoDto-qcQwLengETrfFdct26-MhVVPFEJu79ievdrt87BG0TCZjMA4O214ynpVrWQoh_M_6l7Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19465366</pqid></control><display><type>article</type><title>Delivery of Telomerase Reverse Transcriptase Small Interfering RNA in Complex with Positively Charged Single-Walled Carbon Nanotubes Suppresses Tumor Growth</title><source>MEDLINE</source><source>American Association for Cancer Research</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Zhang, Zhuohan ; Yang, Xiaoying ; Zhang, Yuan ; Zeng, Bin ; Wang, Shujing ; Zhu, Tianhui ; Roden, Richard B S ; Chen, Yongsheng ; Yang, Rongcun</creator><creatorcontrib>Zhang, Zhuohan ; Yang, Xiaoying ; Zhang, Yuan ; Zeng, Bin ; Wang, Shujing ; Zhu, Tianhui ; Roden, Richard B S ; Chen, Yongsheng ; Yang, Rongcun</creatorcontrib><description>Purpose: To determine whether -CONH-(CH 2 ) 6 -NH 3 + Cl − functionalized single-walled carbon nanotubes (SWNT) carrying complexed small interfering RNA (siRNA) can enter into tumor
cells, wherein they release the siRNA to silence the targeted gene.
Experimental Design: -CONH-(CH 2 ) 6 -NH 3 + Cl − was used to mediate the conjugation of telomerase reverse transcriptase (TERT) siRNA to SWNTs. The ability of TERT siRNA
delivered via SWNT complexes to silence the expression of TERT was assessed by their effects on the proliferation and growth
of tumor cells both in vitro and in mouse models.
Results: The functionalized SWNTs -CONH-(CH 2 ) 6 -NH 3 + Cl − could facilitate the coupling of siRNAs that specifically target murine TERT expression to form the mTERT siRNA:SWNT+ complex.
These functionalized SWNTs rapidly entered three cultured murine tumor cell lines, suppressed mTERT expression, and produced
growth arrest. Injection of mTERT siRNA:SWNT+ complexes into s.c. Lewis lung tumors reduced tumor growth. Furthermore, human
TERT siRNA:SWNT+ complexes also suppressed the growth of human HeLa cells both in vitro and when injected into tumors in nude mice.
Conclusions: -CONH-(CH 2 ) 6 -NH 3 + Cl − functionalized SWNTs carry complexed siRNA into tumor cells, wherein they release the siRNA from the nanotube sidewalls to
silence the targeted gene. The -CONH-(CH 2 ) 6 -NH 3 + Cl − functionalized SWNTs may represent a new class of molecular transporters applicable for siRNA therapeutics.</description><identifier>ISSN: 1078-0432</identifier><identifier>EISSN: 1557-3265</identifier><identifier>DOI: 10.1158/1078-0432.CCR-05-2831</identifier><identifier>PMID: 16914582</identifier><language>eng</language><publisher>Philadelphia, PA: American Association for Cancer Research</publisher><subject>Animals ; Antineoplastic agents ; Biological and medical sciences ; Female ; Gene Silencing ; General aspects ; General pharmacology ; HeLa cell ; HeLa Cells ; Humans ; Medical sciences ; Mice ; Nanotubes, Carbon - chemistry ; Neoplasms, Experimental - genetics ; Neoplasms, Experimental - metabolism ; Neoplasms, Experimental - pathology ; Neoplasms, Experimental - therapy ; Pharmaceutical technology. Pharmaceutical industry ; Pharmacology. Drug treatments ; RNA, Small Interfering - administration & dosage ; RNA, Small Interfering - chemistry ; RNA, Small Interfering - genetics ; RNA, Small Interfering - pharmacokinetics ; RNAi ; single-walled carbon nanotubes ; siRNA ; Telomerase - antagonists & inhibitors ; Telomerase - genetics ; telomerase reverse transcriptase ; Transfection</subject><ispartof>Clinical cancer research, 2006-08, Vol.12 (16), p.4933-4939</ispartof><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c501t-14436c65857c124f9577141abd1ca8ed5cb9f5029a233ea31633247a1a059cb33</citedby><cites>FETCH-LOGICAL-c501t-14436c65857c124f9577141abd1ca8ed5cb9f5029a233ea31633247a1a059cb33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,3357,27928,27929</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18052145$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16914582$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Zhuohan</creatorcontrib><creatorcontrib>Yang, Xiaoying</creatorcontrib><creatorcontrib>Zhang, Yuan</creatorcontrib><creatorcontrib>Zeng, Bin</creatorcontrib><creatorcontrib>Wang, Shujing</creatorcontrib><creatorcontrib>Zhu, Tianhui</creatorcontrib><creatorcontrib>Roden, Richard B S</creatorcontrib><creatorcontrib>Chen, Yongsheng</creatorcontrib><creatorcontrib>Yang, Rongcun</creatorcontrib><title>Delivery of Telomerase Reverse Transcriptase Small Interfering RNA in Complex with Positively Charged Single-Walled Carbon Nanotubes Suppresses Tumor Growth</title><title>Clinical cancer research</title><addtitle>Clin Cancer Res</addtitle><description>Purpose: To determine whether -CONH-(CH 2 ) 6 -NH 3 + Cl − functionalized single-walled carbon nanotubes (SWNT) carrying complexed small interfering RNA (siRNA) can enter into tumor
cells, wherein they release the siRNA to silence the targeted gene.
Experimental Design: -CONH-(CH 2 ) 6 -NH 3 + Cl − was used to mediate the conjugation of telomerase reverse transcriptase (TERT) siRNA to SWNTs. The ability of TERT siRNA
delivered via SWNT complexes to silence the expression of TERT was assessed by their effects on the proliferation and growth
of tumor cells both in vitro and in mouse models.
Results: The functionalized SWNTs -CONH-(CH 2 ) 6 -NH 3 + Cl − could facilitate the coupling of siRNAs that specifically target murine TERT expression to form the mTERT siRNA:SWNT+ complex.
These functionalized SWNTs rapidly entered three cultured murine tumor cell lines, suppressed mTERT expression, and produced
growth arrest. Injection of mTERT siRNA:SWNT+ complexes into s.c. Lewis lung tumors reduced tumor growth. Furthermore, human
TERT siRNA:SWNT+ complexes also suppressed the growth of human HeLa cells both in vitro and when injected into tumors in nude mice.
Conclusions: -CONH-(CH 2 ) 6 -NH 3 + Cl − functionalized SWNTs carry complexed siRNA into tumor cells, wherein they release the siRNA from the nanotube sidewalls to
silence the targeted gene. The -CONH-(CH 2 ) 6 -NH 3 + Cl − functionalized SWNTs may represent a new class of molecular transporters applicable for siRNA therapeutics.</description><subject>Animals</subject><subject>Antineoplastic agents</subject><subject>Biological and medical sciences</subject><subject>Female</subject><subject>Gene Silencing</subject><subject>General aspects</subject><subject>General pharmacology</subject><subject>HeLa cell</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Nanotubes, Carbon - chemistry</subject><subject>Neoplasms, Experimental - genetics</subject><subject>Neoplasms, Experimental - metabolism</subject><subject>Neoplasms, Experimental - pathology</subject><subject>Neoplasms, Experimental - therapy</subject><subject>Pharmaceutical technology. Pharmaceutical industry</subject><subject>Pharmacology. Drug treatments</subject><subject>RNA, Small Interfering - administration & dosage</subject><subject>RNA, Small Interfering - chemistry</subject><subject>RNA, Small Interfering - genetics</subject><subject>RNA, Small Interfering - pharmacokinetics</subject><subject>RNAi</subject><subject>single-walled carbon nanotubes</subject><subject>siRNA</subject><subject>Telomerase - antagonists & inhibitors</subject><subject>Telomerase - genetics</subject><subject>telomerase reverse transcriptase</subject><subject>Transfection</subject><issn>1078-0432</issn><issn>1557-3265</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkd1u1DAQhSMEoqXwCCDfgMRFin_zc1kFKJWqgnYXcWk53snGyInDOGHZd-Fh8WoX9WrGo-_MyOdk2WtGrxlT1QdGyyqnUvDrplnlVOW8EuxJdsmUKnPBC_U09f-Zi-xFjD8pZZJR-Ty7YEXNpKr4Zfb3I3j3G_BAQkc24MMAaCKQFaRhqhs0Y7Topvk4XQ_Ge3I3zoAdoBt3ZPVwQ9xImjBMHv6QvZt78i1EN6el_kCa3uAOtmSdWA_5jyRPr8ZgG0byYMYwLy1Esl6mCSHG1G6WISC5xbCf-5fZs874CK_O9Sr7_vnTpvmS33-9vWtu7nOrKJtzJqUobKEqVVrGZVerskw_Ne2WWVPBVtm27hTlteFCgBGsEILL0jBDVW1bIa6yd6e9E4ZfC8RZDy5a8N6MEJaoWS0LJYoigeoEWgwxInR6QjcYPGhG9TEWfbRcHy3XKRZNlT7GknRvzgeWdoDto-qcQwLengETrfFdct26-MhVVPFEJu79ievdrt87BG0TCZjMA4O214ynpVrWQoh_M_6l7Q</recordid><startdate>20060815</startdate><enddate>20060815</enddate><creator>Zhang, Zhuohan</creator><creator>Yang, Xiaoying</creator><creator>Zhang, Yuan</creator><creator>Zeng, Bin</creator><creator>Wang, Shujing</creator><creator>Zhu, Tianhui</creator><creator>Roden, Richard B S</creator><creator>Chen, Yongsheng</creator><creator>Yang, Rongcun</creator><general>American Association for Cancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20060815</creationdate><title>Delivery of Telomerase Reverse Transcriptase Small Interfering RNA in Complex with Positively Charged Single-Walled Carbon Nanotubes Suppresses Tumor Growth</title><author>Zhang, Zhuohan ; Yang, Xiaoying ; Zhang, Yuan ; Zeng, Bin ; Wang, Shujing ; Zhu, Tianhui ; Roden, Richard B S ; Chen, Yongsheng ; Yang, Rongcun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c501t-14436c65857c124f9577141abd1ca8ed5cb9f5029a233ea31633247a1a059cb33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Antineoplastic agents</topic><topic>Biological and medical sciences</topic><topic>Female</topic><topic>Gene Silencing</topic><topic>General aspects</topic><topic>General pharmacology</topic><topic>HeLa cell</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Nanotubes, Carbon - chemistry</topic><topic>Neoplasms, Experimental - genetics</topic><topic>Neoplasms, Experimental - metabolism</topic><topic>Neoplasms, Experimental - pathology</topic><topic>Neoplasms, Experimental - therapy</topic><topic>Pharmaceutical technology. Pharmaceutical industry</topic><topic>Pharmacology. Drug treatments</topic><topic>RNA, Small Interfering - administration & dosage</topic><topic>RNA, Small Interfering - chemistry</topic><topic>RNA, Small Interfering - genetics</topic><topic>RNA, Small Interfering - pharmacokinetics</topic><topic>RNAi</topic><topic>single-walled carbon nanotubes</topic><topic>siRNA</topic><topic>Telomerase - antagonists & inhibitors</topic><topic>Telomerase - genetics</topic><topic>telomerase reverse transcriptase</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Zhuohan</creatorcontrib><creatorcontrib>Yang, Xiaoying</creatorcontrib><creatorcontrib>Zhang, Yuan</creatorcontrib><creatorcontrib>Zeng, Bin</creatorcontrib><creatorcontrib>Wang, Shujing</creatorcontrib><creatorcontrib>Zhu, Tianhui</creatorcontrib><creatorcontrib>Roden, Richard B S</creatorcontrib><creatorcontrib>Chen, Yongsheng</creatorcontrib><creatorcontrib>Yang, Rongcun</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Clinical cancer research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Zhuohan</au><au>Yang, Xiaoying</au><au>Zhang, Yuan</au><au>Zeng, Bin</au><au>Wang, Shujing</au><au>Zhu, Tianhui</au><au>Roden, Richard B S</au><au>Chen, Yongsheng</au><au>Yang, Rongcun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Delivery of Telomerase Reverse Transcriptase Small Interfering RNA in Complex with Positively Charged Single-Walled Carbon Nanotubes Suppresses Tumor Growth</atitle><jtitle>Clinical cancer research</jtitle><addtitle>Clin Cancer Res</addtitle><date>2006-08-15</date><risdate>2006</risdate><volume>12</volume><issue>16</issue><spage>4933</spage><epage>4939</epage><pages>4933-4939</pages><issn>1078-0432</issn><eissn>1557-3265</eissn><abstract>Purpose: To determine whether -CONH-(CH 2 ) 6 -NH 3 + Cl − functionalized single-walled carbon nanotubes (SWNT) carrying complexed small interfering RNA (siRNA) can enter into tumor
cells, wherein they release the siRNA to silence the targeted gene.
Experimental Design: -CONH-(CH 2 ) 6 -NH 3 + Cl − was used to mediate the conjugation of telomerase reverse transcriptase (TERT) siRNA to SWNTs. The ability of TERT siRNA
delivered via SWNT complexes to silence the expression of TERT was assessed by their effects on the proliferation and growth
of tumor cells both in vitro and in mouse models.
Results: The functionalized SWNTs -CONH-(CH 2 ) 6 -NH 3 + Cl − could facilitate the coupling of siRNAs that specifically target murine TERT expression to form the mTERT siRNA:SWNT+ complex.
These functionalized SWNTs rapidly entered three cultured murine tumor cell lines, suppressed mTERT expression, and produced
growth arrest. Injection of mTERT siRNA:SWNT+ complexes into s.c. Lewis lung tumors reduced tumor growth. Furthermore, human
TERT siRNA:SWNT+ complexes also suppressed the growth of human HeLa cells both in vitro and when injected into tumors in nude mice.
Conclusions: -CONH-(CH 2 ) 6 -NH 3 + Cl − functionalized SWNTs carry complexed siRNA into tumor cells, wherein they release the siRNA from the nanotube sidewalls to
silence the targeted gene. The -CONH-(CH 2 ) 6 -NH 3 + Cl − functionalized SWNTs may represent a new class of molecular transporters applicable for siRNA therapeutics.</abstract><cop>Philadelphia, PA</cop><pub>American Association for Cancer Research</pub><pmid>16914582</pmid><doi>10.1158/1078-0432.CCR-05-2831</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; American Association for Cancer Research; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Antineoplastic agents Biological and medical sciences Female Gene Silencing General aspects General pharmacology HeLa cell HeLa Cells Humans Medical sciences Mice Nanotubes, Carbon - chemistry Neoplasms, Experimental - genetics Neoplasms, Experimental - metabolism Neoplasms, Experimental - pathology Neoplasms, Experimental - therapy Pharmaceutical technology. Pharmaceutical industry Pharmacology. Drug treatments RNA, Small Interfering - administration & dosage RNA, Small Interfering - chemistry RNA, Small Interfering - genetics RNA, Small Interfering - pharmacokinetics RNAi single-walled carbon nanotubes siRNA Telomerase - antagonists & inhibitors Telomerase - genetics telomerase reverse transcriptase Transfection |
| title | Delivery of Telomerase Reverse Transcriptase Small Interfering RNA in Complex with Positively Charged Single-Walled Carbon Nanotubes Suppresses Tumor Growth |
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