Singlet Oxygen-Induced DNA Damage
DeFedericis, H-C., Patrzyc, H. B., Rajecki, M. J., Budzinski, E. E., Iijima, H., Dawidzik, J. B., Evans, M. S., Greene, K. F. and Box, H. C. Singlet Oxygen-Induced DNA Damage. Radiat. Res. 165, 445–451 (2006). Singlet oxygen, hydrogen peroxide, hydroxyl radical and hydrogen peroxide are the reactive...
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| Veröffentlicht in: | Radiation research 2006-04, Vol.165 (4), p.445-451 |
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| creator | DeFedericis, Han-Chun Patrzyc, Helen B. Rajecki, Michael J. Budzinski, Edwin E. Iijima, Herbert Dawidzik, Jean B. Evans, Marianne S. Greene, Kellee F. Box, Harold C. |
| description | DeFedericis, H-C., Patrzyc, H. B., Rajecki, M. J., Budzinski, E. E., Iijima, H., Dawidzik, J. B., Evans, M. S., Greene, K. F. and Box, H. C. Singlet Oxygen-Induced DNA Damage. Radiat. Res. 165, 445–451 (2006). Singlet oxygen, hydrogen peroxide, hydroxyl radical and hydrogen peroxide are the reactive oxygen species (ROS) considered most responsible for producing oxidative stress in cells and organisms. Singlet oxygen interacts preferentially with guanine to produce 8-oxo-7,8-dihydroguanine and spiroiminodihydantoin. DNA damage due to the latter lesion has not been detected directly in the DNA of cells exposed to singlet oxygen. In this study, the singlet oxygen-induced lesion was isolated from a short synthetic oligomer after exposure to UVA radiation in the presence of methylene blue. The lesion could be enzymatically excised from the oligomer in the form of a modified dinucleoside monophosphate. Using liquid chromatography-tandem mass spectrometry (LC-MS/MS), the singlet oxygen lesion was detected in the form of modified dinucleoside monophosphates in double-stranded DNA and in the DNA of HeLa cells exposed to singlet oxygen. Pentamer containing the singlet oxygen-induced lesion and an isotopic label was synthesized as an internal standard for quantifying the lesion and served as well as for correcting for losses of product during sample preparation. |
| doi_str_mv | 10.1667/RR3533.1 |
| format | Article |
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B., Rajecki, M. J., Budzinski, E. E., Iijima, H., Dawidzik, J. B., Evans, M. S., Greene, K. F. and Box, H. C. Singlet Oxygen-Induced DNA Damage. Radiat. Res. 165, 445–451 (2006). Singlet oxygen, hydrogen peroxide, hydroxyl radical and hydrogen peroxide are the reactive oxygen species (ROS) considered most responsible for producing oxidative stress in cells and organisms. Singlet oxygen interacts preferentially with guanine to produce 8-oxo-7,8-dihydroguanine and spiroiminodihydantoin. DNA damage due to the latter lesion has not been detected directly in the DNA of cells exposed to singlet oxygen. In this study, the singlet oxygen-induced lesion was isolated from a short synthetic oligomer after exposure to UVA radiation in the presence of methylene blue. The lesion could be enzymatically excised from the oligomer in the form of a modified dinucleoside monophosphate. Using liquid chromatography-tandem mass spectrometry (LC-MS/MS), the singlet oxygen lesion was detected in the form of modified dinucleoside monophosphates in double-stranded DNA and in the DNA of HeLa cells exposed to singlet oxygen. Pentamer containing the singlet oxygen-induced lesion and an isotopic label was synthesized as an internal standard for quantifying the lesion and served as well as for correcting for losses of product during sample preparation.</description><identifier>ISSN: 0033-7587</identifier><identifier>EISSN: 1938-5404</identifier><identifier>DOI: 10.1667/RR3533.1</identifier><identifier>PMID: 16579657</identifier><language>eng</language><publisher>United States: Radiation Research Society</publisher><subject>Animals ; Chromatography, Liquid - methods ; Dinucleoside phosphates ; Dinucleoside Phosphates - chemistry ; Dinucleoside Phosphates - radiation effects ; DNA ; DNA - chemistry ; DNA - genetics ; DNA - radiation effects ; DNA Damage ; Dose-Response Relationship, Radiation ; Elution ; HeLa Cells ; Humans ; Lesions ; Mass Spectrometry - methods ; Mutagenicity Tests - methods ; Nucleosides ; Oligomers ; Oxidation ; Radiation Dosage ; Singlet oxygen ; Singlet Oxygen - chemistry ; Thymine - analogs & derivatives ; Thymine - chemistry ; Thymine - radiation effects</subject><ispartof>Radiation research, 2006-04, Vol.165 (4), p.445-451</ispartof><rights>Radiation Research Society</rights><rights>Copyright 2006 The Radiation Research Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b364t-2f768d8d3e0cf6a8cd816c42f8af391058aa368a374e42b42715918633524d53</citedby><cites>FETCH-LOGICAL-b364t-2f768d8d3e0cf6a8cd816c42f8af391058aa368a374e42b42715918633524d53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://bioone.org/doi/pdf/10.1667/RR3533.1$$EPDF$$P50$$Gbioone$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3581443$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,26978,27924,27925,52363,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16579657$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>DeFedericis, Han-Chun</creatorcontrib><creatorcontrib>Patrzyc, Helen B.</creatorcontrib><creatorcontrib>Rajecki, Michael J.</creatorcontrib><creatorcontrib>Budzinski, Edwin E.</creatorcontrib><creatorcontrib>Iijima, Herbert</creatorcontrib><creatorcontrib>Dawidzik, Jean B.</creatorcontrib><creatorcontrib>Evans, Marianne S.</creatorcontrib><creatorcontrib>Greene, Kellee F.</creatorcontrib><creatorcontrib>Box, Harold C.</creatorcontrib><title>Singlet Oxygen-Induced DNA Damage</title><title>Radiation research</title><addtitle>Radiat Res</addtitle><description>DeFedericis, H-C., Patrzyc, H. B., Rajecki, M. J., Budzinski, E. E., Iijima, H., Dawidzik, J. B., Evans, M. S., Greene, K. F. and Box, H. C. Singlet Oxygen-Induced DNA Damage. Radiat. Res. 165, 445–451 (2006). Singlet oxygen, hydrogen peroxide, hydroxyl radical and hydrogen peroxide are the reactive oxygen species (ROS) considered most responsible for producing oxidative stress in cells and organisms. Singlet oxygen interacts preferentially with guanine to produce 8-oxo-7,8-dihydroguanine and spiroiminodihydantoin. DNA damage due to the latter lesion has not been detected directly in the DNA of cells exposed to singlet oxygen. In this study, the singlet oxygen-induced lesion was isolated from a short synthetic oligomer after exposure to UVA radiation in the presence of methylene blue. The lesion could be enzymatically excised from the oligomer in the form of a modified dinucleoside monophosphate. Using liquid chromatography-tandem mass spectrometry (LC-MS/MS), the singlet oxygen lesion was detected in the form of modified dinucleoside monophosphates in double-stranded DNA and in the DNA of HeLa cells exposed to singlet oxygen. Pentamer containing the singlet oxygen-induced lesion and an isotopic label was synthesized as an internal standard for quantifying the lesion and served as well as for correcting for losses of product during sample preparation.</description><subject>Animals</subject><subject>Chromatography, Liquid - methods</subject><subject>Dinucleoside phosphates</subject><subject>Dinucleoside Phosphates - chemistry</subject><subject>Dinucleoside Phosphates - radiation effects</subject><subject>DNA</subject><subject>DNA - chemistry</subject><subject>DNA - genetics</subject><subject>DNA - radiation effects</subject><subject>DNA Damage</subject><subject>Dose-Response Relationship, Radiation</subject><subject>Elution</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Lesions</subject><subject>Mass Spectrometry - methods</subject><subject>Mutagenicity Tests - methods</subject><subject>Nucleosides</subject><subject>Oligomers</subject><subject>Oxidation</subject><subject>Radiation Dosage</subject><subject>Singlet oxygen</subject><subject>Singlet Oxygen - chemistry</subject><subject>Thymine - analogs & derivatives</subject><subject>Thymine - chemistry</subject><subject>Thymine - radiation effects</subject><issn>0033-7587</issn><issn>1938-5404</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kFtLw0AQhRdRbK2CP0CkvogvqTuZveWxtF4KxULt-7LJbkpKLjWbgP33RlLskw_DMMzHmTOHkFugExBCPq_XyBEncEaGEKEKOKPsnAwpRQwkV3JArrzf0W4GEV2SAQguo66G5OEzK7e5a8ar78PWlcGitG3i7Hj-MR3PTWG27ppcpCb37ubYR2Tz-rKZvQfL1dtiNl0GMQrWBGEqhbLKoqNJKoxKrAKRsDBVJsUIKFfGoFAGJXMsjFkogUegBCIPmeU4Io-97L6uvlrnG11kPnF5bkpXtV5DxARlMuzApx5M6sr72qV6X2eFqQ8aqP5NQ_dpaOjQ-6NmGxfOnsDj-x1w1wM731T13x65Asbw5CnOqqp0_x_6Aauja-Q</recordid><startdate>200604</startdate><enddate>200604</enddate><creator>DeFedericis, Han-Chun</creator><creator>Patrzyc, Helen B.</creator><creator>Rajecki, Michael J.</creator><creator>Budzinski, Edwin E.</creator><creator>Iijima, Herbert</creator><creator>Dawidzik, Jean B.</creator><creator>Evans, Marianne S.</creator><creator>Greene, Kellee F.</creator><creator>Box, Harold C.</creator><general>Radiation Research Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope></search><sort><creationdate>200604</creationdate><title>Singlet Oxygen-Induced DNA Damage</title><author>DeFedericis, Han-Chun ; Patrzyc, Helen B. ; Rajecki, Michael J. ; Budzinski, Edwin E. ; Iijima, Herbert ; Dawidzik, Jean B. ; Evans, Marianne S. ; Greene, Kellee F. ; Box, Harold C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b364t-2f768d8d3e0cf6a8cd816c42f8af391058aa368a374e42b42715918633524d53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Chromatography, Liquid - methods</topic><topic>Dinucleoside phosphates</topic><topic>Dinucleoside Phosphates - chemistry</topic><topic>Dinucleoside Phosphates - radiation effects</topic><topic>DNA</topic><topic>DNA - chemistry</topic><topic>DNA - genetics</topic><topic>DNA - radiation effects</topic><topic>DNA Damage</topic><topic>Dose-Response Relationship, Radiation</topic><topic>Elution</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Lesions</topic><topic>Mass Spectrometry - methods</topic><topic>Mutagenicity Tests - methods</topic><topic>Nucleosides</topic><topic>Oligomers</topic><topic>Oxidation</topic><topic>Radiation Dosage</topic><topic>Singlet oxygen</topic><topic>Singlet Oxygen - chemistry</topic><topic>Thymine - analogs & derivatives</topic><topic>Thymine - chemistry</topic><topic>Thymine - radiation effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DeFedericis, Han-Chun</creatorcontrib><creatorcontrib>Patrzyc, Helen B.</creatorcontrib><creatorcontrib>Rajecki, Michael J.</creatorcontrib><creatorcontrib>Budzinski, Edwin E.</creatorcontrib><creatorcontrib>Iijima, Herbert</creatorcontrib><creatorcontrib>Dawidzik, Jean B.</creatorcontrib><creatorcontrib>Evans, Marianne S.</creatorcontrib><creatorcontrib>Greene, Kellee F.</creatorcontrib><creatorcontrib>Box, Harold C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Radiation research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>DeFedericis, Han-Chun</au><au>Patrzyc, Helen B.</au><au>Rajecki, Michael J.</au><au>Budzinski, Edwin E.</au><au>Iijima, Herbert</au><au>Dawidzik, Jean B.</au><au>Evans, Marianne S.</au><au>Greene, Kellee F.</au><au>Box, Harold C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Singlet Oxygen-Induced DNA Damage</atitle><jtitle>Radiation research</jtitle><addtitle>Radiat Res</addtitle><date>2006-04</date><risdate>2006</risdate><volume>165</volume><issue>4</issue><spage>445</spage><epage>451</epage><pages>445-451</pages><issn>0033-7587</issn><eissn>1938-5404</eissn><abstract>DeFedericis, H-C., Patrzyc, H. B., Rajecki, M. J., Budzinski, E. E., Iijima, H., Dawidzik, J. B., Evans, M. S., Greene, K. F. and Box, H. C. Singlet Oxygen-Induced DNA Damage. Radiat. Res. 165, 445–451 (2006). Singlet oxygen, hydrogen peroxide, hydroxyl radical and hydrogen peroxide are the reactive oxygen species (ROS) considered most responsible for producing oxidative stress in cells and organisms. Singlet oxygen interacts preferentially with guanine to produce 8-oxo-7,8-dihydroguanine and spiroiminodihydantoin. DNA damage due to the latter lesion has not been detected directly in the DNA of cells exposed to singlet oxygen. In this study, the singlet oxygen-induced lesion was isolated from a short synthetic oligomer after exposure to UVA radiation in the presence of methylene blue. The lesion could be enzymatically excised from the oligomer in the form of a modified dinucleoside monophosphate. Using liquid chromatography-tandem mass spectrometry (LC-MS/MS), the singlet oxygen lesion was detected in the form of modified dinucleoside monophosphates in double-stranded DNA and in the DNA of HeLa cells exposed to singlet oxygen. Pentamer containing the singlet oxygen-induced lesion and an isotopic label was synthesized as an internal standard for quantifying the lesion and served as well as for correcting for losses of product during sample preparation.</abstract><cop>United States</cop><pub>Radiation Research Society</pub><pmid>16579657</pmid><doi>10.1667/RR3533.1</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals Chromatography, Liquid - methods Dinucleoside phosphates Dinucleoside Phosphates - chemistry Dinucleoside Phosphates - radiation effects DNA DNA - chemistry DNA - genetics DNA - radiation effects DNA Damage Dose-Response Relationship, Radiation Elution HeLa Cells Humans Lesions Mass Spectrometry - methods Mutagenicity Tests - methods Nucleosides Oligomers Oxidation Radiation Dosage Singlet oxygen Singlet Oxygen - chemistry Thymine - analogs & derivatives Thymine - chemistry Thymine - radiation effects |
| title | Singlet Oxygen-Induced DNA Damage |
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