Distinct Roles of Ventromedial versus Ventrolateral Striatal Medium Spiny Neurons in Reward-Oriented Behavior

The ventral striatum (VS) is a key brain center regulating reward-oriented behavior [1–4]. The VS can be anatomically divided into medial (VMS) and lateral (VLS) portions based on cortical input patterns. The VMS receives inputs from medial pallium-originated limbic structures (e.g., the medial prefrontal cortex [mPFC]), and the VLS receives inputs from the lateral pallium-originated areas (e.g., the insula) [5, 6]. This anatomical feature led us to hypothesize a functional segregation within the VS in terms of the regulation of reward-oriented behavior. Here, we engineered a fiber photometry system [4] and monitored population-level Ca2+ activities of dopamine D2-receptor-expressing medium spiny neurons (D2-MSNs), one of the major cell types in the striatum, during a food-seeking discrimination task. We found that VLS D2-MSNs were activated at the time of cue presentation. In stark contrast, VMS D2-MSNs were inhibited at this time point. Optogenetic counteraction of those changes in the VLS and VMS impaired action initiation and increased responding toward non-rewarded cues, respectively. During lever-press reversal training, VMS inhibition at the time of cue presentation temporarily ceased and optogenetic activation of VMS D2-MSNs facilitated acquisition of the new contingency. These data indicate that the opposing inhibition and excitation in VMS and VLS are important for selecting and initiating a proper action in a reward-oriented behavior. We propose distinct subregional roles within the VS in the execution of successful reward-oriented behavior. [Display omitted] •Opposing activities within the VS were observed during reward-oriented behavior•VLS D2-MSN compound Ca2+ elevations encode action initiation•VMS D2-MSN compound Ca2+ decreases encode action direction toward reward cues•Disinhibition of VMS D2-MSNs facilitates a flexible direction shift Tsutsui-Kimura, Natsubori, et al. find opposing activity patterns of medium spiny neurons at the medial and lateral portions of the ventral striatum during reward-oriented behavior. Each activity mediates distinct function but cooperates with successful reward-oriented behavior, proposing a new functional dichotomy of the ventral striatum.