Removal of 4-chlorophenol by soybean peroxidase and hydrogen peroxide in a discontinuous tank reactor

A discontinuous tank reactor was used to eliminate 4-chlorophenol using soybean peroxidase (SBP) and hydrogen peroxide in an attempt to reduce the concentrations of buffer solutions used and to avoid adding polyethylene glycol. The stirred-tank reactor was operated in different operating conditions,...

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Veröffentlicht in:Desalination 2006-08, Vol.195 (1), p.51-59
Hauptverfasser: Bódalo, Antonio, Gómez, José L., Gómez, Elisa, Hidalgo, Asunción M., Gómez, María, Yelo, Ana M.
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container_end_page 59
container_issue 1
container_start_page 51
container_title Desalination
container_volume 195
creator Bódalo, Antonio
Gómez, José L.
Gómez, Elisa
Hidalgo, Asunción M.
Gómez, María
Yelo, Ana M.
description A discontinuous tank reactor was used to eliminate 4-chlorophenol using soybean peroxidase (SBP) and hydrogen peroxide in an attempt to reduce the concentrations of buffer solutions used and to avoid adding polyethylene glycol. The stirred-tank reactor was operated in different operating conditions, and the influence of enzyme and substrate concentrations on the elimination of 4-chlorophenol was determined. Most of the assays provided good elimination percentages, which reached almost 90% for an enzyme concentration of 7.5·10 –3 mg cm –3 and a stoichiometric ratio of 1:1 for both substrates, when concentrations of 1.5 mmol dm –3 or less were used. This work is a first step in the study of the SBP/4-chlorophenol system in order to observe the influence of the reagents concentrations when no polyethylene glycol is added.
doi_str_mv 10.1016/j.desal.2005.11.017
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subjects 4-chlorophenol
Applied sciences
Chemical engineering
Discontinuous tank reactor
Exact sciences and technology
General purification processes
Hydrogen peroxide
Pollution
Reactors
Soybean peroxidase enzyme
Wastewater treatment
Wastewaters
Water treatment and pollution
title Removal of 4-chlorophenol by soybean peroxidase and hydrogen peroxide in a discontinuous tank reactor
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