Genotyping of Campylobacter coli and C. jejuni from retail chicken meat and humans with campylobacteriosis in Slovenia and Bosnia and Herzegovina
Thermotolerant Campylobacter jejuni and C. coli are one of the major causes of bacterial foodborne enteric infection. Consuming and/or handling poultry meat is the most consistent risk factor, linked to the high prevalence of campylobacters in retail poultry meat. The aim of the present study was to...
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| Veröffentlicht in: | International journal of food microbiology 2006-07, Vol.110 (1), p.24-33 |
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| creator | Zorman, T. Heyndrickx, M. Uzunović-Kamberović, S. Smole Možina, S. |
| description | Thermotolerant
Campylobacter jejuni and
C. coli are one of the major causes of bacterial foodborne enteric infection. Consuming and/or handling poultry meat is the most consistent risk factor, linked to the high prevalence of campylobacters in retail poultry meat. The aim of the present study was to ascertain the genetic diversity and/or possible specificity of thermotolerant
Campylobacter isolates according to species (
C. coli,
C. jejuni), isolation source (retail chicken meat and human clinical samples) and geographic origin (Goriška in Slovenia and Zenica-Doboj Canton in Bosnia and Herzegovina (BIH)). With the pulsed-field gel electrophoresis (PFGE) after
SmaI macrorestriction we distinguished 80 PFGE types among 118 strains and
CfoI restriction fragment length polymorphism of the amplified flagellin gene (
fla-RFLP) gave 12
fla-RFLP types. Beside the higher discriminatory power and strain typeability, PFGE discriminated the
C. jejuni and
C. coli groups of isolates
. A high proportion of
C. coli strains was isolated, especially from poultry samples. Identical or very similar PFGE types among the isolates from animal, food and human samples indicate the transmission of
C. jejuni and
C. coli from the chickens on the farm to the retail chicken meat, as well as possible cross-contamination of retail meat and transmission to humans. However, the identity of the isolates from non-related samples but with identical PFGE and
fla-RFLP types should be confirmed with additional typing. Reliable tracing of the source of
Campylobacter strains by molecular typing of the chicken meat isolates is therefore very difficult. The reasons include contamination of meat samples with multiple strains, possible cross-contamination and extreme heterogeneity of the isolates (mainly for
C. jejuni) on one side and a limited power of the genotyping methods used to distinguish non-related strains on the other side (mainly for
C. coli). |
| doi_str_mv | 10.1016/j.ijfoodmicro.2006.03.001 |
| format | Article |
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Campylobacter jejuni and
C. coli are one of the major causes of bacterial foodborne enteric infection. Consuming and/or handling poultry meat is the most consistent risk factor, linked to the high prevalence of campylobacters in retail poultry meat. The aim of the present study was to ascertain the genetic diversity and/or possible specificity of thermotolerant
Campylobacter isolates according to species (
C. coli,
C. jejuni), isolation source (retail chicken meat and human clinical samples) and geographic origin (Goriška in Slovenia and Zenica-Doboj Canton in Bosnia and Herzegovina (BIH)). With the pulsed-field gel electrophoresis (PFGE) after
SmaI macrorestriction we distinguished 80 PFGE types among 118 strains and
CfoI restriction fragment length polymorphism of the amplified flagellin gene (
fla-RFLP) gave 12
fla-RFLP types. Beside the higher discriminatory power and strain typeability, PFGE discriminated the
C. jejuni and
C. coli groups of isolates
. A high proportion of
C. coli strains was isolated, especially from poultry samples. Identical or very similar PFGE types among the isolates from animal, food and human samples indicate the transmission of
C. jejuni and
C. coli from the chickens on the farm to the retail chicken meat, as well as possible cross-contamination of retail meat and transmission to humans. However, the identity of the isolates from non-related samples but with identical PFGE and
fla-RFLP types should be confirmed with additional typing. Reliable tracing of the source of
Campylobacter strains by molecular typing of the chicken meat isolates is therefore very difficult. The reasons include contamination of meat samples with multiple strains, possible cross-contamination and extreme heterogeneity of the isolates (mainly for
C. jejuni) on one side and a limited power of the genotyping methods used to distinguish non-related strains on the other side (mainly for
C. coli).</description><identifier>ISSN: 0168-1605</identifier><identifier>EISSN: 1879-3460</identifier><identifier>DOI: 10.1016/j.ijfoodmicro.2006.03.001</identifier><identifier>PMID: 16712997</identifier><identifier>CODEN: IJFMDD</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Bosnia and Herzegovina ; Campylobacter coli ; Campylobacter coli - classification ; Campylobacter coli - genetics ; Campylobacter coli - isolation & purification ; Campylobacter Infections - microbiology ; Campylobacter Infections - transmission ; Campylobacter jejuni ; Campylobacter jejuni - classification ; Campylobacter jejuni - genetics ; Campylobacter jejuni - isolation & purification ; Chicken meat ; Chickens ; Consumer Product Safety ; Electrophoresis, Gel, Pulsed-Field ; fla-RFLP ; Food Contamination - analysis ; Food industries ; Food Microbiology ; Fundamental and applied biological sciences. Psychology ; Genotype ; Human campylobacteriosis ; Humans ; Meat - microbiology ; Meat and meat product industries ; PFGE ; Phylogeny ; Polymorphism, Restriction Fragment Length ; Poultry Diseases - microbiology ; Poultry Diseases - transmission ; Slovenia ; Species Specificity</subject><ispartof>International journal of food microbiology, 2006-07, Vol.110 (1), p.24-33</ispartof><rights>2006 Elsevier B.V.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-44b4c7fcde1b58d3a9968df77e2963c3696ec0b943e6e391864a946ee506304d3</citedby><cites>FETCH-LOGICAL-c436t-44b4c7fcde1b58d3a9968df77e2963c3696ec0b943e6e391864a946ee506304d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijfoodmicro.2006.03.001$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17972390$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16712997$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zorman, T.</creatorcontrib><creatorcontrib>Heyndrickx, M.</creatorcontrib><creatorcontrib>Uzunović-Kamberović, S.</creatorcontrib><creatorcontrib>Smole Možina, S.</creatorcontrib><title>Genotyping of Campylobacter coli and C. jejuni from retail chicken meat and humans with campylobacteriosis in Slovenia and Bosnia and Herzegovina</title><title>International journal of food microbiology</title><addtitle>Int J Food Microbiol</addtitle><description>Thermotolerant
Campylobacter jejuni and
C. coli are one of the major causes of bacterial foodborne enteric infection. Consuming and/or handling poultry meat is the most consistent risk factor, linked to the high prevalence of campylobacters in retail poultry meat. The aim of the present study was to ascertain the genetic diversity and/or possible specificity of thermotolerant
Campylobacter isolates according to species (
C. coli,
C. jejuni), isolation source (retail chicken meat and human clinical samples) and geographic origin (Goriška in Slovenia and Zenica-Doboj Canton in Bosnia and Herzegovina (BIH)). With the pulsed-field gel electrophoresis (PFGE) after
SmaI macrorestriction we distinguished 80 PFGE types among 118 strains and
CfoI restriction fragment length polymorphism of the amplified flagellin gene (
fla-RFLP) gave 12
fla-RFLP types. Beside the higher discriminatory power and strain typeability, PFGE discriminated the
C. jejuni and
C. coli groups of isolates
. A high proportion of
C. coli strains was isolated, especially from poultry samples. Identical or very similar PFGE types among the isolates from animal, food and human samples indicate the transmission of
C. jejuni and
C. coli from the chickens on the farm to the retail chicken meat, as well as possible cross-contamination of retail meat and transmission to humans. However, the identity of the isolates from non-related samples but with identical PFGE and
fla-RFLP types should be confirmed with additional typing. Reliable tracing of the source of
Campylobacter strains by molecular typing of the chicken meat isolates is therefore very difficult. The reasons include contamination of meat samples with multiple strains, possible cross-contamination and extreme heterogeneity of the isolates (mainly for
C. jejuni) on one side and a limited power of the genotyping methods used to distinguish non-related strains on the other side (mainly for
C. coli).</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Bosnia and Herzegovina</subject><subject>Campylobacter coli</subject><subject>Campylobacter coli - classification</subject><subject>Campylobacter coli - genetics</subject><subject>Campylobacter coli - isolation & purification</subject><subject>Campylobacter Infections - microbiology</subject><subject>Campylobacter Infections - transmission</subject><subject>Campylobacter jejuni</subject><subject>Campylobacter jejuni - classification</subject><subject>Campylobacter jejuni - genetics</subject><subject>Campylobacter jejuni - isolation & purification</subject><subject>Chicken meat</subject><subject>Chickens</subject><subject>Consumer Product Safety</subject><subject>Electrophoresis, Gel, Pulsed-Field</subject><subject>fla-RFLP</subject><subject>Food Contamination - analysis</subject><subject>Food industries</subject><subject>Food Microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genotype</subject><subject>Human campylobacteriosis</subject><subject>Humans</subject><subject>Meat - microbiology</subject><subject>Meat and meat product industries</subject><subject>PFGE</subject><subject>Phylogeny</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Poultry Diseases - microbiology</subject><subject>Poultry Diseases - transmission</subject><subject>Slovenia</subject><subject>Species Specificity</subject><issn>0168-1605</issn><issn>1879-3460</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0cFu1DAQBmALgehSeAVkDnBLsGPHiY8Q0RapEgfgbDnOpDshsRc72Wp5C964aXdRe-TkOXwzY81PyDvOcs64-jjkOPQhdBO6GPKCMZUzkTPGn5ENryudCanYc7JZbZ1xxcoz8iqlgTFWCsFekjOuKl5oXW3I30vwYT7s0N_Q0NPGTrvDGFrrZojUhRGp9R1tcjrAsHikfQwTjTBbHKnbovsFnk5g5we2XSbrE73FeUvd00kYEiaKnn4fwx482gf-OaR_5RXEP3AT9ujta_Kit2OCN6f3nPy8-PKjucquv11-bT5dZ04KNWdSttJVveuAt2XdCau1qru-qqDQSjihtALHWi0FKBCa10paLRVAyZRgshPn5MNx7i6G3wuk2UyYHIyj9RCWZLiWpaxlsUJ9hOuxU4rQm13EycaD4czc52EG8yQPc5-HYcKseay9b09LlnaC7rHzFMAK3p-ATc6OfbTeYXp0la4KodnqmqOD9SR7hGiSQ_AOOozgZtMF_I_v3AEdAbF-</recordid><startdate>20060701</startdate><enddate>20060701</enddate><creator>Zorman, T.</creator><creator>Heyndrickx, M.</creator><creator>Uzunović-Kamberović, S.</creator><creator>Smole Možina, S.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T2</scope><scope>7U2</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20060701</creationdate><title>Genotyping of Campylobacter coli and C. jejuni from retail chicken meat and humans with campylobacteriosis in Slovenia and Bosnia and Herzegovina</title><author>Zorman, T. ; Heyndrickx, M. ; Uzunović-Kamberović, S. ; Smole Možina, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-44b4c7fcde1b58d3a9968df77e2963c3696ec0b943e6e391864a946ee506304d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Bosnia and Herzegovina</topic><topic>Campylobacter coli</topic><topic>Campylobacter coli - classification</topic><topic>Campylobacter coli - genetics</topic><topic>Campylobacter coli - isolation & purification</topic><topic>Campylobacter Infections - microbiology</topic><topic>Campylobacter Infections - transmission</topic><topic>Campylobacter jejuni</topic><topic>Campylobacter jejuni - classification</topic><topic>Campylobacter jejuni - genetics</topic><topic>Campylobacter jejuni - isolation & purification</topic><topic>Chicken meat</topic><topic>Chickens</topic><topic>Consumer Product Safety</topic><topic>Electrophoresis, Gel, Pulsed-Field</topic><topic>fla-RFLP</topic><topic>Food Contamination - analysis</topic><topic>Food industries</topic><topic>Food Microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genotype</topic><topic>Human campylobacteriosis</topic><topic>Humans</topic><topic>Meat - microbiology</topic><topic>Meat and meat product industries</topic><topic>PFGE</topic><topic>Phylogeny</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Poultry Diseases - microbiology</topic><topic>Poultry Diseases - transmission</topic><topic>Slovenia</topic><topic>Species Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zorman, T.</creatorcontrib><creatorcontrib>Heyndrickx, M.</creatorcontrib><creatorcontrib>Uzunović-Kamberović, S.</creatorcontrib><creatorcontrib>Smole Možina, S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Safety Science and Risk</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>International journal of food microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zorman, T.</au><au>Heyndrickx, M.</au><au>Uzunović-Kamberović, S.</au><au>Smole Možina, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genotyping of Campylobacter coli and C. jejuni from retail chicken meat and humans with campylobacteriosis in Slovenia and Bosnia and Herzegovina</atitle><jtitle>International journal of food microbiology</jtitle><addtitle>Int J Food Microbiol</addtitle><date>2006-07-01</date><risdate>2006</risdate><volume>110</volume><issue>1</issue><spage>24</spage><epage>33</epage><pages>24-33</pages><issn>0168-1605</issn><eissn>1879-3460</eissn><coden>IJFMDD</coden><abstract>Thermotolerant
Campylobacter jejuni and
C. coli are one of the major causes of bacterial foodborne enteric infection. Consuming and/or handling poultry meat is the most consistent risk factor, linked to the high prevalence of campylobacters in retail poultry meat. The aim of the present study was to ascertain the genetic diversity and/or possible specificity of thermotolerant
Campylobacter isolates according to species (
C. coli,
C. jejuni), isolation source (retail chicken meat and human clinical samples) and geographic origin (Goriška in Slovenia and Zenica-Doboj Canton in Bosnia and Herzegovina (BIH)). With the pulsed-field gel electrophoresis (PFGE) after
SmaI macrorestriction we distinguished 80 PFGE types among 118 strains and
CfoI restriction fragment length polymorphism of the amplified flagellin gene (
fla-RFLP) gave 12
fla-RFLP types. Beside the higher discriminatory power and strain typeability, PFGE discriminated the
C. jejuni and
C. coli groups of isolates
. A high proportion of
C. coli strains was isolated, especially from poultry samples. Identical or very similar PFGE types among the isolates from animal, food and human samples indicate the transmission of
C. jejuni and
C. coli from the chickens on the farm to the retail chicken meat, as well as possible cross-contamination of retail meat and transmission to humans. However, the identity of the isolates from non-related samples but with identical PFGE and
fla-RFLP types should be confirmed with additional typing. Reliable tracing of the source of
Campylobacter strains by molecular typing of the chicken meat isolates is therefore very difficult. The reasons include contamination of meat samples with multiple strains, possible cross-contamination and extreme heterogeneity of the isolates (mainly for
C. jejuni) on one side and a limited power of the genotyping methods used to distinguish non-related strains on the other side (mainly for
C. coli).</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>16712997</pmid><doi>10.1016/j.ijfoodmicro.2006.03.001</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0168-1605 |
| ispartof | International journal of food microbiology, 2006-07, Vol.110 (1), p.24-33 |
| issn | 0168-1605 1879-3460 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Biological and medical sciences Bosnia and Herzegovina Campylobacter coli Campylobacter coli - classification Campylobacter coli - genetics Campylobacter coli - isolation & purification Campylobacter Infections - microbiology Campylobacter Infections - transmission Campylobacter jejuni Campylobacter jejuni - classification Campylobacter jejuni - genetics Campylobacter jejuni - isolation & purification Chicken meat Chickens Consumer Product Safety Electrophoresis, Gel, Pulsed-Field fla-RFLP Food Contamination - analysis Food industries Food Microbiology Fundamental and applied biological sciences. Psychology Genotype Human campylobacteriosis Humans Meat - microbiology Meat and meat product industries PFGE Phylogeny Polymorphism, Restriction Fragment Length Poultry Diseases - microbiology Poultry Diseases - transmission Slovenia Species Specificity |
| title | Genotyping of Campylobacter coli and C. jejuni from retail chicken meat and humans with campylobacteriosis in Slovenia and Bosnia and Herzegovina |
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