Dimorphic expression of sex-related genes in different gonadal development stages of sterlet, Acipenser ruthenus, a primitive fish species
Molecular mechanism of sex determination and differentiation of sturgeon, a primitive fish species, is extraordinarily important due to the valuable caviar; however, it is still poorly known. The present work aimed to identify the major genes involved in regulating gonadal development of sterlet, a...
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| Veröffentlicht in: | Fish physiology and biochemistry 2017-12, Vol.43 (6), p.1557-1569 |
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| creator | Wang, Wei Zhu, Hua Dong, Ying Tian, ZhaoHui Dong, Tian Hu, HongXia Niu, CuiJuan |
| description | Molecular mechanism of sex determination and differentiation of sturgeon, a primitive fish species, is extraordinarily important due to the valuable caviar; however, it is still poorly known. The present work aimed to identify the major genes involved in regulating gonadal development of sterlet, a small species of sturgeon, from 13 candidate genes which have been shown to relate to gonadal differentiation and development in other teleost fish. The sex and gonadal development of sterlets were determined by histological observation and levels of sex steroids testosterone (T), 11-ketotestosterone (11-KT), and 17β-estradiol (E2) in serum. Sexually dimorphic gene expressions were investigated. The results revealed that gonadal development were asynchronous in 2-year-old male and female sterlets with the testes in early or mid-spermatogenesis and the ovaries in chromatin nucleolus stage or perinucleolus stage, respectively. The levels of T and E2 were not significantly different between sexes or different gonadal development stages while 11-KT had the higher level in mid-spermatogenesis testis stage. In all the investigated gonadal development stages, gene
dmrt1
and
hsd11b2
were expressed higher in male whereas
foxl2
and
cyp19a1
were expressed higher in female. Thus, these genes provided the promising markers for sex identification of sterlet. It was unexpected that
dkk1
and
dax1
had significantly higher expression in ovarian perinucleolus stage than in ovarian chromatin nucleolus stage and in the testis, suggesting that these two genes had more correlation with ovarian development than with the testis, contrary to the previous reports in other vertebrates. Testicular development-related genes (
gsdf
and
amh
) and estrogen receptor genes (
era
and
erb
) differentially expressed at different testis or ovary development stages, but their expressions were not absolutely significantly different in male and female, depending on the gonadal development stage. Expression of androgen receptor gene
ar
or
rspo
, which was supposed to be related to ovarian development, presented no difference between gonadal development stages investigated in this study whenever in male or female. |
| doi_str_mv | 10.1007/s10695-017-0392-x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1945216991</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1951232433</sourcerecordid><originalsourceid>FETCH-LOGICAL-c372t-b0fa4a4d5afaa8f198bc9b237728d3a1aad0882a790be0231d444e6f71cb4f443</originalsourceid><addsrcrecordid>eNp1kcFu1DAQhi0EokvhAbggS1w4NOCxnTg-Vi0UpEpc4Gw5yXjXVeIET1Itr8BTk2gLqpA4WRp__z-2PsZeg3gPQpgPBKKyZSHAFEJZWRyfsB2URhUlVPVTthNWigKMlmfsBdGdEMKaCp6zM1nbSlUGduzXdRzGPB1iy_E4ZSSKY-Jj4ITHImPvZ-z4HhMSj4l3MQTMmGa-H5PvfM87vMd-nIZtRrPfr9wWnjH3OF_wyzZOmAgzz8t8wLTQBfd8ynGIc7xHHiIdOE3YRqSX7FnwPeGrh_Ocff_08dvV5-L2682Xq8vbolVGzkUjgtded6UP3tcBbN20tpHKGFl3yoP3nahr6Y0VDQqpoNNaYxUMtI0OWqtz9u7UO-Xxx4I0uyFSi33vE44LObC6lFBZCyv69h_0blxyWl-3UiVIJbVSKwUnqs0jUcbgtg_6_NOBcJsodxLlVlFuE-WOa-bNQ_PSDNj9TfwxswLyBNB6lfaYH63-b-tvjWOhKw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1951232433</pqid></control><display><type>article</type><title>Dimorphic expression of sex-related genes in different gonadal development stages of sterlet, Acipenser ruthenus, a primitive fish species</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Wang, Wei ; Zhu, Hua ; Dong, Ying ; Tian, ZhaoHui ; Dong, Tian ; Hu, HongXia ; Niu, CuiJuan</creator><creatorcontrib>Wang, Wei ; Zhu, Hua ; Dong, Ying ; Tian, ZhaoHui ; Dong, Tian ; Hu, HongXia ; Niu, CuiJuan</creatorcontrib><description>Molecular mechanism of sex determination and differentiation of sturgeon, a primitive fish species, is extraordinarily important due to the valuable caviar; however, it is still poorly known. The present work aimed to identify the major genes involved in regulating gonadal development of sterlet, a small species of sturgeon, from 13 candidate genes which have been shown to relate to gonadal differentiation and development in other teleost fish. The sex and gonadal development of sterlets were determined by histological observation and levels of sex steroids testosterone (T), 11-ketotestosterone (11-KT), and 17β-estradiol (E2) in serum. Sexually dimorphic gene expressions were investigated. The results revealed that gonadal development were asynchronous in 2-year-old male and female sterlets with the testes in early or mid-spermatogenesis and the ovaries in chromatin nucleolus stage or perinucleolus stage, respectively. The levels of T and E2 were not significantly different between sexes or different gonadal development stages while 11-KT had the higher level in mid-spermatogenesis testis stage. In all the investigated gonadal development stages, gene
dmrt1
and
hsd11b2
were expressed higher in male whereas
foxl2
and
cyp19a1
were expressed higher in female. Thus, these genes provided the promising markers for sex identification of sterlet. It was unexpected that
dkk1
and
dax1
had significantly higher expression in ovarian perinucleolus stage than in ovarian chromatin nucleolus stage and in the testis, suggesting that these two genes had more correlation with ovarian development than with the testis, contrary to the previous reports in other vertebrates. Testicular development-related genes (
gsdf
and
amh
) and estrogen receptor genes (
era
and
erb
) differentially expressed at different testis or ovary development stages, but their expressions were not absolutely significantly different in male and female, depending on the gonadal development stage. Expression of androgen receptor gene
ar
or
rspo
, which was supposed to be related to ovarian development, presented no difference between gonadal development stages investigated in this study whenever in male or female.</description><identifier>ISSN: 0920-1742</identifier><identifier>EISSN: 1573-5168</identifier><identifier>DOI: 10.1007/s10695-017-0392-x</identifier><identifier>PMID: 28963671</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>17β-Estradiol ; Acipenser ruthenus ; Androgen receptors ; Animal Anatomy ; Animal Biochemistry ; Animal Physiology ; Animal reproductive organs ; Animals ; Biomedical and Life Sciences ; Caviar ; Chromatin ; Developmental stages ; Differentiation ; Dkk1 protein ; Estradiol - blood ; Estrogen receptors ; Estrogens ; Female ; Females ; Fish ; Fishes - blood ; Fishes - genetics ; Fishes - physiology ; Freshwater & Marine Ecology ; Freshwater fishes ; Gene expression ; Genes ; Histology ; Life Sciences ; Male ; Males ; Morphology ; Nucleoli ; Oestrogens ; Ovaries ; Serum ; Sex ; Sex determination ; Sex Factors ; Sex hormones ; Sexual Development - genetics ; Sexual Development - physiology ; Sexual dimorphism ; Species ; Species Specificity ; Spermatogenesis ; Steroid hormones ; Steroids ; Sturgeon ; Testes ; Testosterone ; Testosterone - analogs & derivatives ; Testosterone - blood ; Vertebrates ; Water temperature ; Zoology</subject><ispartof>Fish physiology and biochemistry, 2017-12, Vol.43 (6), p.1557-1569</ispartof><rights>Springer Science+Business Media Dordrecht 2017</rights><rights>Fish Physiology and Biochemistry is a copyright of Springer, 2017.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-b0fa4a4d5afaa8f198bc9b237728d3a1aad0882a790be0231d444e6f71cb4f443</citedby><cites>FETCH-LOGICAL-c372t-b0fa4a4d5afaa8f198bc9b237728d3a1aad0882a790be0231d444e6f71cb4f443</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10695-017-0392-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10695-017-0392-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28963671$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Wei</creatorcontrib><creatorcontrib>Zhu, Hua</creatorcontrib><creatorcontrib>Dong, Ying</creatorcontrib><creatorcontrib>Tian, ZhaoHui</creatorcontrib><creatorcontrib>Dong, Tian</creatorcontrib><creatorcontrib>Hu, HongXia</creatorcontrib><creatorcontrib>Niu, CuiJuan</creatorcontrib><title>Dimorphic expression of sex-related genes in different gonadal development stages of sterlet, Acipenser ruthenus, a primitive fish species</title><title>Fish physiology and biochemistry</title><addtitle>Fish Physiol Biochem</addtitle><addtitle>Fish Physiol Biochem</addtitle><description>Molecular mechanism of sex determination and differentiation of sturgeon, a primitive fish species, is extraordinarily important due to the valuable caviar; however, it is still poorly known. The present work aimed to identify the major genes involved in regulating gonadal development of sterlet, a small species of sturgeon, from 13 candidate genes which have been shown to relate to gonadal differentiation and development in other teleost fish. The sex and gonadal development of sterlets were determined by histological observation and levels of sex steroids testosterone (T), 11-ketotestosterone (11-KT), and 17β-estradiol (E2) in serum. Sexually dimorphic gene expressions were investigated. The results revealed that gonadal development were asynchronous in 2-year-old male and female sterlets with the testes in early or mid-spermatogenesis and the ovaries in chromatin nucleolus stage or perinucleolus stage, respectively. The levels of T and E2 were not significantly different between sexes or different gonadal development stages while 11-KT had the higher level in mid-spermatogenesis testis stage. In all the investigated gonadal development stages, gene
dmrt1
and
hsd11b2
were expressed higher in male whereas
foxl2
and
cyp19a1
were expressed higher in female. Thus, these genes provided the promising markers for sex identification of sterlet. It was unexpected that
dkk1
and
dax1
had significantly higher expression in ovarian perinucleolus stage than in ovarian chromatin nucleolus stage and in the testis, suggesting that these two genes had more correlation with ovarian development than with the testis, contrary to the previous reports in other vertebrates. Testicular development-related genes (
gsdf
and
amh
) and estrogen receptor genes (
era
and
erb
) differentially expressed at different testis or ovary development stages, but their expressions were not absolutely significantly different in male and female, depending on the gonadal development stage. Expression of androgen receptor gene
ar
or
rspo
, which was supposed to be related to ovarian development, presented no difference between gonadal development stages investigated in this study whenever in male or female.</description><subject>17β-Estradiol</subject><subject>Acipenser ruthenus</subject><subject>Androgen receptors</subject><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Animal Physiology</subject><subject>Animal reproductive organs</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Caviar</subject><subject>Chromatin</subject><subject>Developmental stages</subject><subject>Differentiation</subject><subject>Dkk1 protein</subject><subject>Estradiol - blood</subject><subject>Estrogen receptors</subject><subject>Estrogens</subject><subject>Female</subject><subject>Females</subject><subject>Fish</subject><subject>Fishes - blood</subject><subject>Fishes - genetics</subject><subject>Fishes - physiology</subject><subject>Freshwater & Marine Ecology</subject><subject>Freshwater fishes</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Histology</subject><subject>Life Sciences</subject><subject>Male</subject><subject>Males</subject><subject>Morphology</subject><subject>Nucleoli</subject><subject>Oestrogens</subject><subject>Ovaries</subject><subject>Serum</subject><subject>Sex</subject><subject>Sex determination</subject><subject>Sex Factors</subject><subject>Sex hormones</subject><subject>Sexual Development - genetics</subject><subject>Sexual Development - physiology</subject><subject>Sexual dimorphism</subject><subject>Species</subject><subject>Species Specificity</subject><subject>Spermatogenesis</subject><subject>Steroid hormones</subject><subject>Steroids</subject><subject>Sturgeon</subject><subject>Testes</subject><subject>Testosterone</subject><subject>Testosterone - analogs & derivatives</subject><subject>Testosterone - blood</subject><subject>Vertebrates</subject><subject>Water temperature</subject><subject>Zoology</subject><issn>0920-1742</issn><issn>1573-5168</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kcFu1DAQhi0EokvhAbggS1w4NOCxnTg-Vi0UpEpc4Gw5yXjXVeIET1Itr8BTk2gLqpA4WRp__z-2PsZeg3gPQpgPBKKyZSHAFEJZWRyfsB2URhUlVPVTthNWigKMlmfsBdGdEMKaCp6zM1nbSlUGduzXdRzGPB1iy_E4ZSSKY-Jj4ITHImPvZ-z4HhMSj4l3MQTMmGa-H5PvfM87vMd-nIZtRrPfr9wWnjH3OF_wyzZOmAgzz8t8wLTQBfd8ynGIc7xHHiIdOE3YRqSX7FnwPeGrh_Ocff_08dvV5-L2682Xq8vbolVGzkUjgtded6UP3tcBbN20tpHKGFl3yoP3nahr6Y0VDQqpoNNaYxUMtI0OWqtz9u7UO-Xxx4I0uyFSi33vE44LObC6lFBZCyv69h_0blxyWl-3UiVIJbVSKwUnqs0jUcbgtg_6_NOBcJsodxLlVlFuE-WOa-bNQ_PSDNj9TfwxswLyBNB6lfaYH63-b-tvjWOhKw</recordid><startdate>20171201</startdate><enddate>20171201</enddate><creator>Wang, Wei</creator><creator>Zhu, Hua</creator><creator>Dong, Ying</creator><creator>Tian, ZhaoHui</creator><creator>Dong, Tian</creator><creator>Hu, HongXia</creator><creator>Niu, CuiJuan</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QH</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7TN</scope><scope>7U7</scope><scope>7UA</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L.F</scope><scope>L.G</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20171201</creationdate><title>Dimorphic expression of sex-related genes in different gonadal development stages of sterlet, Acipenser ruthenus, a primitive fish species</title><author>Wang, Wei ; Zhu, Hua ; Dong, Ying ; Tian, ZhaoHui ; Dong, Tian ; Hu, HongXia ; Niu, CuiJuan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-b0fa4a4d5afaa8f198bc9b237728d3a1aad0882a790be0231d444e6f71cb4f443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>17β-Estradiol</topic><topic>Acipenser ruthenus</topic><topic>Androgen receptors</topic><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Animal Physiology</topic><topic>Animal reproductive organs</topic><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Caviar</topic><topic>Chromatin</topic><topic>Developmental stages</topic><topic>Differentiation</topic><topic>Dkk1 protein</topic><topic>Estradiol - blood</topic><topic>Estrogen receptors</topic><topic>Estrogens</topic><topic>Female</topic><topic>Females</topic><topic>Fish</topic><topic>Fishes - blood</topic><topic>Fishes - genetics</topic><topic>Fishes - physiology</topic><topic>Freshwater & Marine Ecology</topic><topic>Freshwater fishes</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Histology</topic><topic>Life Sciences</topic><topic>Male</topic><topic>Males</topic><topic>Morphology</topic><topic>Nucleoli</topic><topic>Oestrogens</topic><topic>Ovaries</topic><topic>Serum</topic><topic>Sex</topic><topic>Sex determination</topic><topic>Sex Factors</topic><topic>Sex hormones</topic><topic>Sexual Development - genetics</topic><topic>Sexual Development - physiology</topic><topic>Sexual dimorphism</topic><topic>Species</topic><topic>Species Specificity</topic><topic>Spermatogenesis</topic><topic>Steroid hormones</topic><topic>Steroids</topic><topic>Sturgeon</topic><topic>Testes</topic><topic>Testosterone</topic><topic>Testosterone - analogs & derivatives</topic><topic>Testosterone - blood</topic><topic>Vertebrates</topic><topic>Water temperature</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Wei</creatorcontrib><creatorcontrib>Zhu, Hua</creatorcontrib><creatorcontrib>Dong, Ying</creatorcontrib><creatorcontrib>Tian, ZhaoHui</creatorcontrib><creatorcontrib>Dong, Tian</creatorcontrib><creatorcontrib>Hu, HongXia</creatorcontrib><creatorcontrib>Niu, CuiJuan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aqualine</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Fish physiology and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Wei</au><au>Zhu, Hua</au><au>Dong, Ying</au><au>Tian, ZhaoHui</au><au>Dong, Tian</au><au>Hu, HongXia</au><au>Niu, CuiJuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dimorphic expression of sex-related genes in different gonadal development stages of sterlet, Acipenser ruthenus, a primitive fish species</atitle><jtitle>Fish physiology and biochemistry</jtitle><stitle>Fish Physiol Biochem</stitle><addtitle>Fish Physiol Biochem</addtitle><date>2017-12-01</date><risdate>2017</risdate><volume>43</volume><issue>6</issue><spage>1557</spage><epage>1569</epage><pages>1557-1569</pages><issn>0920-1742</issn><eissn>1573-5168</eissn><abstract>Molecular mechanism of sex determination and differentiation of sturgeon, a primitive fish species, is extraordinarily important due to the valuable caviar; however, it is still poorly known. The present work aimed to identify the major genes involved in regulating gonadal development of sterlet, a small species of sturgeon, from 13 candidate genes which have been shown to relate to gonadal differentiation and development in other teleost fish. The sex and gonadal development of sterlets were determined by histological observation and levels of sex steroids testosterone (T), 11-ketotestosterone (11-KT), and 17β-estradiol (E2) in serum. Sexually dimorphic gene expressions were investigated. The results revealed that gonadal development were asynchronous in 2-year-old male and female sterlets with the testes in early or mid-spermatogenesis and the ovaries in chromatin nucleolus stage or perinucleolus stage, respectively. The levels of T and E2 were not significantly different between sexes or different gonadal development stages while 11-KT had the higher level in mid-spermatogenesis testis stage. In all the investigated gonadal development stages, gene
dmrt1
and
hsd11b2
were expressed higher in male whereas
foxl2
and
cyp19a1
were expressed higher in female. Thus, these genes provided the promising markers for sex identification of sterlet. It was unexpected that
dkk1
and
dax1
had significantly higher expression in ovarian perinucleolus stage than in ovarian chromatin nucleolus stage and in the testis, suggesting that these two genes had more correlation with ovarian development than with the testis, contrary to the previous reports in other vertebrates. Testicular development-related genes (
gsdf
and
amh
) and estrogen receptor genes (
era
and
erb
) differentially expressed at different testis or ovary development stages, but their expressions were not absolutely significantly different in male and female, depending on the gonadal development stage. Expression of androgen receptor gene
ar
or
rspo
, which was supposed to be related to ovarian development, presented no difference between gonadal development stages investigated in this study whenever in male or female.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>28963671</pmid><doi>10.1007/s10695-017-0392-x</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Fish physiology and biochemistry, 2017-12, Vol.43 (6), p.1557-1569 |
| issn | 0920-1742 1573-5168 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals |
| subjects | 17β-Estradiol Acipenser ruthenus Androgen receptors Animal Anatomy Animal Biochemistry Animal Physiology Animal reproductive organs Animals Biomedical and Life Sciences Caviar Chromatin Developmental stages Differentiation Dkk1 protein Estradiol - blood Estrogen receptors Estrogens Female Females Fish Fishes - blood Fishes - genetics Fishes - physiology Freshwater & Marine Ecology Freshwater fishes Gene expression Genes Histology Life Sciences Male Males Morphology Nucleoli Oestrogens Ovaries Serum Sex Sex determination Sex Factors Sex hormones Sexual Development - genetics Sexual Development - physiology Sexual dimorphism Species Species Specificity Spermatogenesis Steroid hormones Steroids Sturgeon Testes Testosterone Testosterone - analogs & derivatives Testosterone - blood Vertebrates Water temperature Zoology |
| title | Dimorphic expression of sex-related genes in different gonadal development stages of sterlet, Acipenser ruthenus, a primitive fish species |
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