Biochemical and genetic analysis of a unique poly(ADP-ribosyl) glycohydrolase (PARG) of the pathogenic fungus Fusarium oxysporum f. sp. lycopersici
The genome sequence of the plant pathogen Fusarium oxysporum f. sp. lycopersici contains a single gene encoding a predicted poly(ADP-ribose) glycohydrolase (FOXG_05947.2, PARG). Here, we assessed whether this gene has a role as a global regulator of DNA repair or in virulence as an ADP ribosylating...
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| Veröffentlicht in: | Antonie van Leeuwenhoek 2018-02, Vol.111 (2), p.285-295 |
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| container_title | Antonie van Leeuwenhoek |
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| creator | Araiza-Cervantes, Carlos A. Meza-Carmen, Víctor Martínez-Cadena, Guadalupe Roncero, M. Isabel G. Reyna-López, Georgina E. Franco, Bernardo |
| description | The genome sequence of the plant pathogen
Fusarium oxysporum
f. sp.
lycopersici
contains a single gene encoding a predicted poly(ADP-ribose) glycohydrolase (FOXG_05947.2, PARG). Here, we assessed whether this gene has a role as a global regulator of DNA repair or in virulence as an ADP ribosylating toxin homologue of bacteria. The PARG protein was purified after expressing its encoding gene in
Escherichia coli
. Its inhibition by 6,9-diamino-2-ethoxyacridine lactate monohydrate and tannins was similar to its human orthologue that is involved in DNA repair. A deletion strain of
F. oxysporum
f. sp.
lycopersici
showed no growth defects and was not affected in pathogenicity. Together, our results indicate that the PARG protein of
F. oxysporum
f. sp.
lycopersici
is involved in DNA repair and does not act in pathogenicity as an effector. |
| doi_str_mv | 10.1007/s10482-017-0951-2 |
| format | Article |
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Fusarium oxysporum
f. sp.
lycopersici
contains a single gene encoding a predicted poly(ADP-ribose) glycohydrolase (FOXG_05947.2, PARG). Here, we assessed whether this gene has a role as a global regulator of DNA repair or in virulence as an ADP ribosylating toxin homologue of bacteria. The PARG protein was purified after expressing its encoding gene in
Escherichia coli
. Its inhibition by 6,9-diamino-2-ethoxyacridine lactate monohydrate and tannins was similar to its human orthologue that is involved in DNA repair. A deletion strain of
F. oxysporum
f. sp.
lycopersici
showed no growth defects and was not affected in pathogenicity. Together, our results indicate that the PARG protein of
F. oxysporum
f. sp.
lycopersici
is involved in DNA repair and does not act in pathogenicity as an effector.</description><identifier>ISSN: 0003-6072</identifier><identifier>EISSN: 1572-9699</identifier><identifier>DOI: 10.1007/s10482-017-0951-2</identifier><identifier>PMID: 28956188</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Adenosine diphosphate ; Bacteria ; Biomedical and Life Sciences ; Deoxyribonucleic acid ; DNA ; DNA repair ; E coli ; Fusarium ; Fusarium oxysporum ; Genetic analysis ; Genomes ; Homology ; Lactic acid ; Life Sciences ; Medical Microbiology ; Microbiology ; Nucleotide sequence ; Original Paper ; Pathogenicity ; Pathogens ; Plant Sciences ; Poly(ADP-ribose) ; Poly(ADP-ribose) glycohydrolase ; Repair ; Ribose ; Soil Science & Conservation ; Tannins ; Toxins ; Virulence</subject><ispartof>Antonie van Leeuwenhoek, 2018-02, Vol.111 (2), p.285-295</ispartof><rights>Springer International Publishing AG 2017</rights><rights>Antonie van Leeuwenhoek is a copyright of Springer, (2017). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-a518dc9a654479b1e27c2784bec08a7c0af00da3578eb9c49f3fbcb0fef2266f3</citedby><cites>FETCH-LOGICAL-c372t-a518dc9a654479b1e27c2784bec08a7c0af00da3578eb9c49f3fbcb0fef2266f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10482-017-0951-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10482-017-0951-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28956188$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Araiza-Cervantes, Carlos A.</creatorcontrib><creatorcontrib>Meza-Carmen, Víctor</creatorcontrib><creatorcontrib>Martínez-Cadena, Guadalupe</creatorcontrib><creatorcontrib>Roncero, M. Isabel G.</creatorcontrib><creatorcontrib>Reyna-López, Georgina E.</creatorcontrib><creatorcontrib>Franco, Bernardo</creatorcontrib><title>Biochemical and genetic analysis of a unique poly(ADP-ribosyl) glycohydrolase (PARG) of the pathogenic fungus Fusarium oxysporum f. sp. lycopersici</title><title>Antonie van Leeuwenhoek</title><addtitle>Antonie van Leeuwenhoek</addtitle><addtitle>Antonie Van Leeuwenhoek</addtitle><description>The genome sequence of the plant pathogen
Fusarium oxysporum
f. sp.
lycopersici
contains a single gene encoding a predicted poly(ADP-ribose) glycohydrolase (FOXG_05947.2, PARG). Here, we assessed whether this gene has a role as a global regulator of DNA repair or in virulence as an ADP ribosylating toxin homologue of bacteria. The PARG protein was purified after expressing its encoding gene in
Escherichia coli
. Its inhibition by 6,9-diamino-2-ethoxyacridine lactate monohydrate and tannins was similar to its human orthologue that is involved in DNA repair. A deletion strain of
F. oxysporum
f. sp.
lycopersici
showed no growth defects and was not affected in pathogenicity. Together, our results indicate that the PARG protein of
F. oxysporum
f. sp.
lycopersici
is involved in DNA repair and does not act in pathogenicity as an effector.</description><subject>Adenosine diphosphate</subject><subject>Bacteria</subject><subject>Biomedical and Life Sciences</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA repair</subject><subject>E coli</subject><subject>Fusarium</subject><subject>Fusarium oxysporum</subject><subject>Genetic analysis</subject><subject>Genomes</subject><subject>Homology</subject><subject>Lactic acid</subject><subject>Life Sciences</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Nucleotide sequence</subject><subject>Original Paper</subject><subject>Pathogenicity</subject><subject>Pathogens</subject><subject>Plant Sciences</subject><subject>Poly(ADP-ribose)</subject><subject>Poly(ADP-ribose) glycohydrolase</subject><subject>Repair</subject><subject>Ribose</subject><subject>Soil Science & Conservation</subject><subject>Tannins</subject><subject>Toxins</subject><subject>Virulence</subject><issn>0003-6072</issn><issn>1572-9699</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp1kd1uFCEYhonR2G31AjwxJJ5sD1iB-QEOt9VWkyY2Ro8Jw8Auzcww8s0kznV4wzLZ1hgTj_gIz_sQeBF6w-iOUSreA6Ol5IQyQaiqGOHP0IZVghNVK_UcbSilBamp4GfoHOAhb1UtxUt0xqWqaiblBv26CtEeXR-s6bAZWnxwg5uCzbPpFgiAo8cGz0P4MTs8xm7Z7j_ckxSaCEt3iQ_dYuNxaVPsDDi8vd9_vb1cM9Mx42Y6xizMOj8PhxnwzQwmhbnH8ecCY0x58jsM4w6vntElCDa8Qi-86cC9flwv0Pebj9-uP5G7L7efr_d3xBaCT8RUTLZWmboqS6Ea5riwXMiycZZKIyw1ntLWFJWQrlG2VL7wjW2od57zuvbFBdqevGOK-XUw6T6AdV1nBhdn0EyVZVnUXLKMvvsHfYhzyl-0Uqqg9Yplip0omyJAcl6PKfQmLZpRvTamT43p3JheG9M8Z94-muemd-2fxFNFGeAnAPLRcHDpr6v_a_0NPq6igA</recordid><startdate>20180201</startdate><enddate>20180201</enddate><creator>Araiza-Cervantes, Carlos A.</creator><creator>Meza-Carmen, Víctor</creator><creator>Martínez-Cadena, Guadalupe</creator><creator>Roncero, M. Isabel G.</creator><creator>Reyna-López, Georgina E.</creator><creator>Franco, Bernardo</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20180201</creationdate><title>Biochemical and genetic analysis of a unique poly(ADP-ribosyl) glycohydrolase (PARG) of the pathogenic fungus Fusarium oxysporum f. sp. lycopersici</title><author>Araiza-Cervantes, Carlos A. ; Meza-Carmen, Víctor ; Martínez-Cadena, Guadalupe ; Roncero, M. Isabel G. ; Reyna-López, Georgina E. ; Franco, Bernardo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-a518dc9a654479b1e27c2784bec08a7c0af00da3578eb9c49f3fbcb0fef2266f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Adenosine diphosphate</topic><topic>Bacteria</topic><topic>Biomedical and Life Sciences</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA repair</topic><topic>E coli</topic><topic>Fusarium</topic><topic>Fusarium oxysporum</topic><topic>Genetic analysis</topic><topic>Genomes</topic><topic>Homology</topic><topic>Lactic acid</topic><topic>Life Sciences</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Nucleotide sequence</topic><topic>Original Paper</topic><topic>Pathogenicity</topic><topic>Pathogens</topic><topic>Plant Sciences</topic><topic>Poly(ADP-ribose)</topic><topic>Poly(ADP-ribose) glycohydrolase</topic><topic>Repair</topic><topic>Ribose</topic><topic>Soil Science & Conservation</topic><topic>Tannins</topic><topic>Toxins</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Araiza-Cervantes, Carlos A.</creatorcontrib><creatorcontrib>Meza-Carmen, Víctor</creatorcontrib><creatorcontrib>Martínez-Cadena, Guadalupe</creatorcontrib><creatorcontrib>Roncero, M. Isabel G.</creatorcontrib><creatorcontrib>Reyna-López, Georgina E.</creatorcontrib><creatorcontrib>Franco, Bernardo</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Antonie van Leeuwenhoek</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Araiza-Cervantes, Carlos A.</au><au>Meza-Carmen, Víctor</au><au>Martínez-Cadena, Guadalupe</au><au>Roncero, M. Isabel G.</au><au>Reyna-López, Georgina E.</au><au>Franco, Bernardo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biochemical and genetic analysis of a unique poly(ADP-ribosyl) glycohydrolase (PARG) of the pathogenic fungus Fusarium oxysporum f. sp. lycopersici</atitle><jtitle>Antonie van Leeuwenhoek</jtitle><stitle>Antonie van Leeuwenhoek</stitle><addtitle>Antonie Van Leeuwenhoek</addtitle><date>2018-02-01</date><risdate>2018</risdate><volume>111</volume><issue>2</issue><spage>285</spage><epage>295</epage><pages>285-295</pages><issn>0003-6072</issn><eissn>1572-9699</eissn><abstract>The genome sequence of the plant pathogen
Fusarium oxysporum
f. sp.
lycopersici
contains a single gene encoding a predicted poly(ADP-ribose) glycohydrolase (FOXG_05947.2, PARG). Here, we assessed whether this gene has a role as a global regulator of DNA repair or in virulence as an ADP ribosylating toxin homologue of bacteria. The PARG protein was purified after expressing its encoding gene in
Escherichia coli
. Its inhibition by 6,9-diamino-2-ethoxyacridine lactate monohydrate and tannins was similar to its human orthologue that is involved in DNA repair. A deletion strain of
F. oxysporum
f. sp.
lycopersici
showed no growth defects and was not affected in pathogenicity. Together, our results indicate that the PARG protein of
F. oxysporum
f. sp.
lycopersici
is involved in DNA repair and does not act in pathogenicity as an effector.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>28956188</pmid><doi>10.1007/s10482-017-0951-2</doi><tpages>11</tpages></addata></record> |
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| ispartof | Antonie van Leeuwenhoek, 2018-02, Vol.111 (2), p.285-295 |
| issn | 0003-6072 1572-9699 |
| language | eng |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Adenosine diphosphate Bacteria Biomedical and Life Sciences Deoxyribonucleic acid DNA DNA repair E coli Fusarium Fusarium oxysporum Genetic analysis Genomes Homology Lactic acid Life Sciences Medical Microbiology Microbiology Nucleotide sequence Original Paper Pathogenicity Pathogens Plant Sciences Poly(ADP-ribose) Poly(ADP-ribose) glycohydrolase Repair Ribose Soil Science & Conservation Tannins Toxins Virulence |
| title | Biochemical and genetic analysis of a unique poly(ADP-ribosyl) glycohydrolase (PARG) of the pathogenic fungus Fusarium oxysporum f. sp. lycopersici |
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