Reactive Oxygen Scavenging Effect of Enzymatic Extracts from Sargassum thunbergii
The free radical scavenging activity of water soluble natural antioxidants from Sargassum thunbergii, which is a brown marine alga, was evaluated by examining the radical scavenging activities of the extracts of hydrolyzates from S. thunbergii on hydroxyl, 1,1-diphenyl-2-picrylhydrazyl (DPPH), and a...
Gespeichert in:
| Veröffentlicht in: | Journal of agricultural and food chemistry 2005-08, Vol.53 (17), p.6666-6672 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 6672 |
|---|---|
| container_issue | 17 |
| container_start_page | 6666 |
| container_title | Journal of agricultural and food chemistry |
| container_volume | 53 |
| creator | Park, Pyo-Jam Heo, Soo-Jin Park, Eun-Ju Kim, Se-Kwon Byun, Hee-Guk Jeon, Byong-Tae Jeon, You-Jin |
| description | The free radical scavenging activity of water soluble natural antioxidants from Sargassum thunbergii, which is a brown marine alga, was evaluated by examining the radical scavenging activities of the extracts of hydrolyzates from S. thunbergii on hydroxyl, 1,1-diphenyl-2-picrylhydrazyl (DPPH), and alkyl radicals. A spin-trapping electron spin resonance (ESR) spectrometer was employed, and the results were compared for their ESR signal intensity. S. thunbergii was enzymatically hydrolyzed to prepare water soluble extracts by five carbohydrases (AMG, Celluclast, Termamyl, Ultraflo, and Viscozyme) and proteases (Alcalase, Flavorzyme, Kojizyme, Neutrase, and Protamex). The scavenging activity of the radicals increased with increased concentrations of the extracts. The scavenging results were higher for hydroxyl and alkyl radicals and lower for DPPH radical as compared with vitamin C as a reference. The hydrogen peroxide scavenging activity of the extracts was also investigated; the Alcalase extract showed the highest scavenging activity among the extracts prepared with the five proteases and five carbohydrates. In addition, the DNA damage was determined by using the comet assay with alkaline electrophoresis and was quantified by measuring the tail length. The preventive effect of Alcalase extract from S. thunbergii against DNA damage increased with increments of concentration of the enzymatic extracts. Keywords: Enzymatic extract; Sargassum thunbergii; marine alga; electron spin resonance; radical scavenging activity; comet assay |
| doi_str_mv | 10.1021/jf050582+ |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_19438881</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>19438881</sourcerecordid><originalsourceid>FETCH-LOGICAL-a433t-437726f6a163138d725cf2d56f57fa24cc4aa51d36b3498494ab019a053e517e3</originalsourceid><addsrcrecordid>eNpl0E1v1DAQBmALgehSOPAHIIeCkFDA4-8cUbW0SCu1dFs4WrNeO2TZJMVOql1-Pa6y0AMnH-aZV-OXkJdAPwBl8HETqKTSsPePyAwko6UEMI_JjOZhaaSCI_IspQ2l1EhNn5IjUECFNmxGvl55dENz54uL3b72XbF0eOe7uunqYh6Cd0PRh2Le_d63ODSumO-GmBdSEWLfFkuMNaY0tsXwY-xWPtZN85w8CbhN_sXhPSY3n-fXp-fl4uLsy-mnRYmC86EUXGumgkJQHLhZayZdYGupgtQBmXBOIEpYc7XiojKiEriiUCGV3EvQnh-Tt1Pubex_jT4Ntm2S89stdr4fk4VKcGMMZPhugi72KUUf7G1sWox7C9Te92f_9pfpq0PmuGr9-gEe-srgzQFgcrgNETvXpAenqWKC3btyck0a_O7fHONPqzTX0l5fLq2-_P5NXFXKLrJ_PfmAvcU65sybJaPAaT6QgaRZnEwCXbKbfoxdLvf_H_wB0GSZ6g</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19438881</pqid></control><display><type>article</type><title>Reactive Oxygen Scavenging Effect of Enzymatic Extracts from Sargassum thunbergii</title><source>MEDLINE</source><source>American Chemical Society Journals</source><creator>Park, Pyo-Jam ; Heo, Soo-Jin ; Park, Eun-Ju ; Kim, Se-Kwon ; Byun, Hee-Guk ; Jeon, Byong-Tae ; Jeon, You-Jin</creator><creatorcontrib>Park, Pyo-Jam ; Heo, Soo-Jin ; Park, Eun-Ju ; Kim, Se-Kwon ; Byun, Hee-Guk ; Jeon, Byong-Tae ; Jeon, You-Jin</creatorcontrib><description>The free radical scavenging activity of water soluble natural antioxidants from Sargassum thunbergii, which is a brown marine alga, was evaluated by examining the radical scavenging activities of the extracts of hydrolyzates from S. thunbergii on hydroxyl, 1,1-diphenyl-2-picrylhydrazyl (DPPH), and alkyl radicals. A spin-trapping electron spin resonance (ESR) spectrometer was employed, and the results were compared for their ESR signal intensity. S. thunbergii was enzymatically hydrolyzed to prepare water soluble extracts by five carbohydrases (AMG, Celluclast, Termamyl, Ultraflo, and Viscozyme) and proteases (Alcalase, Flavorzyme, Kojizyme, Neutrase, and Protamex). The scavenging activity of the radicals increased with increased concentrations of the extracts. The scavenging results were higher for hydroxyl and alkyl radicals and lower for DPPH radical as compared with vitamin C as a reference. The hydrogen peroxide scavenging activity of the extracts was also investigated; the Alcalase extract showed the highest scavenging activity among the extracts prepared with the five proteases and five carbohydrates. In addition, the DNA damage was determined by using the comet assay with alkaline electrophoresis and was quantified by measuring the tail length. The preventive effect of Alcalase extract from S. thunbergii against DNA damage increased with increments of concentration of the enzymatic extracts. Keywords: Enzymatic extract; Sargassum thunbergii; marine alga; electron spin resonance; radical scavenging activity; comet assay</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf050582+</identifier><identifier>PMID: 16104782</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>antioxidant activity ; Biological and medical sciences ; DNA Damage ; electron paramagnetic resonance spectroscopy ; Electron Spin Resonance Spectroscopy ; enzymatic hydrolysis ; extracts ; Food industries ; Free Radical Scavengers ; free radical scavenging activity ; free radicals ; Fundamental and applied biological sciences. Psychology ; Glycoside Hydrolases - metabolism ; Humans ; hydrogen peroxide ; Hydrogen Peroxide - metabolism ; hydrolysates ; natural antioxidants ; O-glycoside hydrolases ; proteinases ; reactive oxygen species ; Reactive Oxygen Species - metabolism ; Sargassum ; Sargassum - enzymology ; Sargassum thunbergii</subject><ispartof>Journal of agricultural and food chemistry, 2005-08, Vol.53 (17), p.6666-6672</ispartof><rights>Copyright © 2005 American Chemical Society</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a433t-437726f6a163138d725cf2d56f57fa24cc4aa51d36b3498494ab019a053e517e3</citedby><cites>FETCH-LOGICAL-a433t-437726f6a163138d725cf2d56f57fa24cc4aa51d36b3498494ab019a053e517e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf050582+$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf050582+$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17062422$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16104782$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Park, Pyo-Jam</creatorcontrib><creatorcontrib>Heo, Soo-Jin</creatorcontrib><creatorcontrib>Park, Eun-Ju</creatorcontrib><creatorcontrib>Kim, Se-Kwon</creatorcontrib><creatorcontrib>Byun, Hee-Guk</creatorcontrib><creatorcontrib>Jeon, Byong-Tae</creatorcontrib><creatorcontrib>Jeon, You-Jin</creatorcontrib><title>Reactive Oxygen Scavenging Effect of Enzymatic Extracts from Sargassum thunbergii</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>The free radical scavenging activity of water soluble natural antioxidants from Sargassum thunbergii, which is a brown marine alga, was evaluated by examining the radical scavenging activities of the extracts of hydrolyzates from S. thunbergii on hydroxyl, 1,1-diphenyl-2-picrylhydrazyl (DPPH), and alkyl radicals. A spin-trapping electron spin resonance (ESR) spectrometer was employed, and the results were compared for their ESR signal intensity. S. thunbergii was enzymatically hydrolyzed to prepare water soluble extracts by five carbohydrases (AMG, Celluclast, Termamyl, Ultraflo, and Viscozyme) and proteases (Alcalase, Flavorzyme, Kojizyme, Neutrase, and Protamex). The scavenging activity of the radicals increased with increased concentrations of the extracts. The scavenging results were higher for hydroxyl and alkyl radicals and lower for DPPH radical as compared with vitamin C as a reference. The hydrogen peroxide scavenging activity of the extracts was also investigated; the Alcalase extract showed the highest scavenging activity among the extracts prepared with the five proteases and five carbohydrates. In addition, the DNA damage was determined by using the comet assay with alkaline electrophoresis and was quantified by measuring the tail length. The preventive effect of Alcalase extract from S. thunbergii against DNA damage increased with increments of concentration of the enzymatic extracts. Keywords: Enzymatic extract; Sargassum thunbergii; marine alga; electron spin resonance; radical scavenging activity; comet assay</description><subject>antioxidant activity</subject><subject>Biological and medical sciences</subject><subject>DNA Damage</subject><subject>electron paramagnetic resonance spectroscopy</subject><subject>Electron Spin Resonance Spectroscopy</subject><subject>enzymatic hydrolysis</subject><subject>extracts</subject><subject>Food industries</subject><subject>Free Radical Scavengers</subject><subject>free radical scavenging activity</subject><subject>free radicals</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycoside Hydrolases - metabolism</subject><subject>Humans</subject><subject>hydrogen peroxide</subject><subject>Hydrogen Peroxide - metabolism</subject><subject>hydrolysates</subject><subject>natural antioxidants</subject><subject>O-glycoside hydrolases</subject><subject>proteinases</subject><subject>reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Sargassum</subject><subject>Sargassum - enzymology</subject><subject>Sargassum thunbergii</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpl0E1v1DAQBmALgehSOPAHIIeCkFDA4-8cUbW0SCu1dFs4WrNeO2TZJMVOql1-Pa6y0AMnH-aZV-OXkJdAPwBl8HETqKTSsPePyAwko6UEMI_JjOZhaaSCI_IspQ2l1EhNn5IjUECFNmxGvl55dENz54uL3b72XbF0eOe7uunqYh6Cd0PRh2Le_d63ODSumO-GmBdSEWLfFkuMNaY0tsXwY-xWPtZN85w8CbhN_sXhPSY3n-fXp-fl4uLsy-mnRYmC86EUXGumgkJQHLhZayZdYGupgtQBmXBOIEpYc7XiojKiEriiUCGV3EvQnh-Tt1Pubex_jT4Ntm2S89stdr4fk4VKcGMMZPhugi72KUUf7G1sWox7C9Te92f_9pfpq0PmuGr9-gEe-srgzQFgcrgNETvXpAenqWKC3btyck0a_O7fHONPqzTX0l5fLq2-_P5NXFXKLrJ_PfmAvcU65sybJaPAaT6QgaRZnEwCXbKbfoxdLvf_H_wB0GSZ6g</recordid><startdate>20050824</startdate><enddate>20050824</enddate><creator>Park, Pyo-Jam</creator><creator>Heo, Soo-Jin</creator><creator>Park, Eun-Ju</creator><creator>Kim, Se-Kwon</creator><creator>Byun, Hee-Guk</creator><creator>Jeon, Byong-Tae</creator><creator>Jeon, You-Jin</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TN</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>20050824</creationdate><title>Reactive Oxygen Scavenging Effect of Enzymatic Extracts from Sargassum thunbergii</title><author>Park, Pyo-Jam ; Heo, Soo-Jin ; Park, Eun-Ju ; Kim, Se-Kwon ; Byun, Hee-Guk ; Jeon, Byong-Tae ; Jeon, You-Jin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a433t-437726f6a163138d725cf2d56f57fa24cc4aa51d36b3498494ab019a053e517e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>antioxidant activity</topic><topic>Biological and medical sciences</topic><topic>DNA Damage</topic><topic>electron paramagnetic resonance spectroscopy</topic><topic>Electron Spin Resonance Spectroscopy</topic><topic>enzymatic hydrolysis</topic><topic>extracts</topic><topic>Food industries</topic><topic>Free Radical Scavengers</topic><topic>free radical scavenging activity</topic><topic>free radicals</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycoside Hydrolases - metabolism</topic><topic>Humans</topic><topic>hydrogen peroxide</topic><topic>Hydrogen Peroxide - metabolism</topic><topic>hydrolysates</topic><topic>natural antioxidants</topic><topic>O-glycoside hydrolases</topic><topic>proteinases</topic><topic>reactive oxygen species</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Sargassum</topic><topic>Sargassum - enzymology</topic><topic>Sargassum thunbergii</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Park, Pyo-Jam</creatorcontrib><creatorcontrib>Heo, Soo-Jin</creatorcontrib><creatorcontrib>Park, Eun-Ju</creatorcontrib><creatorcontrib>Kim, Se-Kwon</creatorcontrib><creatorcontrib>Byun, Hee-Guk</creatorcontrib><creatorcontrib>Jeon, Byong-Tae</creatorcontrib><creatorcontrib>Jeon, You-Jin</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oceanic Abstracts</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Park, Pyo-Jam</au><au>Heo, Soo-Jin</au><au>Park, Eun-Ju</au><au>Kim, Se-Kwon</au><au>Byun, Hee-Guk</au><au>Jeon, Byong-Tae</au><au>Jeon, You-Jin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Reactive Oxygen Scavenging Effect of Enzymatic Extracts from Sargassum thunbergii</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2005-08-24</date><risdate>2005</risdate><volume>53</volume><issue>17</issue><spage>6666</spage><epage>6672</epage><pages>6666-6672</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>The free radical scavenging activity of water soluble natural antioxidants from Sargassum thunbergii, which is a brown marine alga, was evaluated by examining the radical scavenging activities of the extracts of hydrolyzates from S. thunbergii on hydroxyl, 1,1-diphenyl-2-picrylhydrazyl (DPPH), and alkyl radicals. A spin-trapping electron spin resonance (ESR) spectrometer was employed, and the results were compared for their ESR signal intensity. S. thunbergii was enzymatically hydrolyzed to prepare water soluble extracts by five carbohydrases (AMG, Celluclast, Termamyl, Ultraflo, and Viscozyme) and proteases (Alcalase, Flavorzyme, Kojizyme, Neutrase, and Protamex). The scavenging activity of the radicals increased with increased concentrations of the extracts. The scavenging results were higher for hydroxyl and alkyl radicals and lower for DPPH radical as compared with vitamin C as a reference. The hydrogen peroxide scavenging activity of the extracts was also investigated; the Alcalase extract showed the highest scavenging activity among the extracts prepared with the five proteases and five carbohydrates. In addition, the DNA damage was determined by using the comet assay with alkaline electrophoresis and was quantified by measuring the tail length. The preventive effect of Alcalase extract from S. thunbergii against DNA damage increased with increments of concentration of the enzymatic extracts. Keywords: Enzymatic extract; Sargassum thunbergii; marine alga; electron spin resonance; radical scavenging activity; comet assay</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>16104782</pmid><doi>10.1021/jf050582+</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-8561 |
| ispartof | Journal of agricultural and food chemistry, 2005-08, Vol.53 (17), p.6666-6672 |
| issn | 0021-8561 1520-5118 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_19438881 |
| source | MEDLINE; American Chemical Society Journals |
| subjects | antioxidant activity Biological and medical sciences DNA Damage electron paramagnetic resonance spectroscopy Electron Spin Resonance Spectroscopy enzymatic hydrolysis extracts Food industries Free Radical Scavengers free radical scavenging activity free radicals Fundamental and applied biological sciences. Psychology Glycoside Hydrolases - metabolism Humans hydrogen peroxide Hydrogen Peroxide - metabolism hydrolysates natural antioxidants O-glycoside hydrolases proteinases reactive oxygen species Reactive Oxygen Species - metabolism Sargassum Sargassum - enzymology Sargassum thunbergii |
| title | Reactive Oxygen Scavenging Effect of Enzymatic Extracts from Sargassum thunbergii |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T01%3A04%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Reactive%20Oxygen%20Scavenging%20Effect%20of%20Enzymatic%20Extracts%20from%20Sargassum%20thunbergii&rft.jtitle=Journal%20of%20agricultural%20and%20food%20chemistry&rft.au=Park,%20Pyo-Jam&rft.date=2005-08-24&rft.volume=53&rft.issue=17&rft.spage=6666&rft.epage=6672&rft.pages=6666-6672&rft.issn=0021-8561&rft.eissn=1520-5118&rft.coden=JAFCAU&rft_id=info:doi/10.1021/jf050582+&rft_dat=%3Cproquest_cross%3E19438881%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19438881&rft_id=info:pmid/16104782&rfr_iscdi=true |