Effects of transgenic overexpression of diapause hormone and diapause hormone receptor genes on non-diapause silkworm
Diapause is a state of developmental arrest that is most often observed in arthropods, especially insects. The domesticated silkworm, Bombyx mori , is a typical insect that enters diapause at an early embryonic stage. Previous studies have revealed that the diapause hormone (DH) signaling molecules,...
Gespeichert in:
| Veröffentlicht in: | Transgenic research 2017-12, Vol.26 (6), p.807-815 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 815 |
|---|---|
| container_issue | 6 |
| container_start_page | 807 |
| container_title | Transgenic research |
| container_volume | 26 |
| creator | Gong, Chunying Zeng, Wenhui Zhang, Tianyang Liu, Rongpeng Ou, Yao Ai, Junwen Xiang, Zhonghuai Xu, Hanfu |
| description | Diapause is a state of developmental arrest that is most often observed in arthropods, especially insects. The domesticated silkworm,
Bombyx mori
, is a typical insect that enters diapause at an early embryonic stage. Previous studies have revealed that the diapause hormone (DH) signaling molecules, especially the core members DH and DH receptor 1 (DHR1), are crucial for the determination of embryonic diapause in diapause silkworm strains. However, whether they function in non-diapause silkworm strains remains largely unknown. Here, we generated two transgenic lines overexpressing
DH
or
DHR1
genes in a non-diapause silkworm strain,
Nistari
. Our results showed that developmental expression patterns of
DH
and
DHR1
are quite similar in transgenic silkworms: both genes are highly expressed in the mid to late stages of pupae and are most highly expressed in day-6 pupae but are expressed at very low levels in other developmental stages. Moreover, the overexpression of
DH
or
DHR1
can affect the expression of diapause-related genes but is not sufficient to induce embryonic diapause in their offspring. This study provides new insights into the function of DH and DHR1 in a non-diapause silkworm strain. |
| doi_str_mv | 10.1007/s11248-017-0045-y |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1943641335</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1943641335</sourcerecordid><originalsourceid>FETCH-LOGICAL-c372t-7159f986d0596d685c15e4f6fc1cafab95c0d5a0299d007a57d93a9113a6be03</originalsourceid><addsrcrecordid>eNp1kc1OAyEURonR2Fp9ADdmEjduUBgGZlgaU3-SJm66J5S51KktjDCj9u2laW2MxhUJ93yHGz6Ezim5poSUN5HSvKgwoSUmpOB4fYCGlJcMSyaqQzQkUuS4qqgcoJMYF4SkVMWO0SCvJM-JKIaoH1sLpouZt1kXtItzcI3J_DsE-GwDxNh4txnWjW51HyF78WHlHWTa1X8vAxhoOx-ypIEkdZnzDu-x2CxfPxJ6io6sXkY4250jNL0fT-8e8eT54enudoINK_MOl5RLKytREy5FLSpuKIfCCmuo0VbPJDek5prkUtbpPzQva8m0pJRpMQPCRuhqq22Df-shdmrVRAPLpXbg-6ioLJgoKGM8oZe_0IXvg0vLJUoIVpQkF4miW8oEH2MAq9rQrHRYK0rUphK1rUSlStSmErVOmYuduZ-toN4nvjtIQL4FYhq5OYQfT_9r_QLpHJjd</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1966347026</pqid></control><display><type>article</type><title>Effects of transgenic overexpression of diapause hormone and diapause hormone receptor genes on non-diapause silkworm</title><source>Springer Nature - Complete Springer Journals</source><creator>Gong, Chunying ; Zeng, Wenhui ; Zhang, Tianyang ; Liu, Rongpeng ; Ou, Yao ; Ai, Junwen ; Xiang, Zhonghuai ; Xu, Hanfu</creator><creatorcontrib>Gong, Chunying ; Zeng, Wenhui ; Zhang, Tianyang ; Liu, Rongpeng ; Ou, Yao ; Ai, Junwen ; Xiang, Zhonghuai ; Xu, Hanfu</creatorcontrib><description>Diapause is a state of developmental arrest that is most often observed in arthropods, especially insects. The domesticated silkworm,
Bombyx mori
, is a typical insect that enters diapause at an early embryonic stage. Previous studies have revealed that the diapause hormone (DH) signaling molecules, especially the core members DH and DH receptor 1 (DHR1), are crucial for the determination of embryonic diapause in diapause silkworm strains. However, whether they function in non-diapause silkworm strains remains largely unknown. Here, we generated two transgenic lines overexpressing
DH
or
DHR1
genes in a non-diapause silkworm strain,
Nistari
. Our results showed that developmental expression patterns of
DH
and
DHR1
are quite similar in transgenic silkworms: both genes are highly expressed in the mid to late stages of pupae and are most highly expressed in day-6 pupae but are expressed at very low levels in other developmental stages. Moreover, the overexpression of
DH
or
DHR1
can affect the expression of diapause-related genes but is not sufficient to induce embryonic diapause in their offspring. This study provides new insights into the function of DH and DHR1 in a non-diapause silkworm strain.</description><identifier>ISSN: 0962-8819</identifier><identifier>EISSN: 1573-9368</identifier><identifier>DOI: 10.1007/s11248-017-0045-y</identifier><identifier>PMID: 28952064</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Animal Genetics and Genomics ; Arthropods ; Biomedical and Life Sciences ; Biomedical Engineering/Biotechnology ; Bombyx mori ; Brief Communication ; Developmental stages ; Diapause ; Embryos ; Gene expression ; Genetic Engineering ; Hormones ; Insects ; Laboratories ; Life Sciences ; Molecular Medicine ; Offspring ; Ovaries ; Plant Genetics and Genomics ; Silkworms ; Transgenics</subject><ispartof>Transgenic research, 2017-12, Vol.26 (6), p.807-815</ispartof><rights>Springer International Publishing AG 2017</rights><rights>Transgenic Research is a copyright of Springer, (2017). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-7159f986d0596d685c15e4f6fc1cafab95c0d5a0299d007a57d93a9113a6be03</citedby><cites>FETCH-LOGICAL-c372t-7159f986d0596d685c15e4f6fc1cafab95c0d5a0299d007a57d93a9113a6be03</cites><orcidid>0000-0002-9239-0362</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11248-017-0045-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11248-017-0045-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51298</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28952064$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gong, Chunying</creatorcontrib><creatorcontrib>Zeng, Wenhui</creatorcontrib><creatorcontrib>Zhang, Tianyang</creatorcontrib><creatorcontrib>Liu, Rongpeng</creatorcontrib><creatorcontrib>Ou, Yao</creatorcontrib><creatorcontrib>Ai, Junwen</creatorcontrib><creatorcontrib>Xiang, Zhonghuai</creatorcontrib><creatorcontrib>Xu, Hanfu</creatorcontrib><title>Effects of transgenic overexpression of diapause hormone and diapause hormone receptor genes on non-diapause silkworm</title><title>Transgenic research</title><addtitle>Transgenic Res</addtitle><addtitle>Transgenic Res</addtitle><description>Diapause is a state of developmental arrest that is most often observed in arthropods, especially insects. The domesticated silkworm,
Bombyx mori
, is a typical insect that enters diapause at an early embryonic stage. Previous studies have revealed that the diapause hormone (DH) signaling molecules, especially the core members DH and DH receptor 1 (DHR1), are crucial for the determination of embryonic diapause in diapause silkworm strains. However, whether they function in non-diapause silkworm strains remains largely unknown. Here, we generated two transgenic lines overexpressing
DH
or
DHR1
genes in a non-diapause silkworm strain,
Nistari
. Our results showed that developmental expression patterns of
DH
and
DHR1
are quite similar in transgenic silkworms: both genes are highly expressed in the mid to late stages of pupae and are most highly expressed in day-6 pupae but are expressed at very low levels in other developmental stages. Moreover, the overexpression of
DH
or
DHR1
can affect the expression of diapause-related genes but is not sufficient to induce embryonic diapause in their offspring. This study provides new insights into the function of DH and DHR1 in a non-diapause silkworm strain.</description><subject>Animal Genetics and Genomics</subject><subject>Arthropods</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>Bombyx mori</subject><subject>Brief Communication</subject><subject>Developmental stages</subject><subject>Diapause</subject><subject>Embryos</subject><subject>Gene expression</subject><subject>Genetic Engineering</subject><subject>Hormones</subject><subject>Insects</subject><subject>Laboratories</subject><subject>Life Sciences</subject><subject>Molecular Medicine</subject><subject>Offspring</subject><subject>Ovaries</subject><subject>Plant Genetics and Genomics</subject><subject>Silkworms</subject><subject>Transgenics</subject><issn>0962-8819</issn><issn>1573-9368</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kc1OAyEURonR2Fp9ADdmEjduUBgGZlgaU3-SJm66J5S51KktjDCj9u2laW2MxhUJ93yHGz6Ezim5poSUN5HSvKgwoSUmpOB4fYCGlJcMSyaqQzQkUuS4qqgcoJMYF4SkVMWO0SCvJM-JKIaoH1sLpouZt1kXtItzcI3J_DsE-GwDxNh4txnWjW51HyF78WHlHWTa1X8vAxhoOx-ypIEkdZnzDu-x2CxfPxJ6io6sXkY4250jNL0fT-8e8eT54enudoINK_MOl5RLKytREy5FLSpuKIfCCmuo0VbPJDek5prkUtbpPzQva8m0pJRpMQPCRuhqq22Df-shdmrVRAPLpXbg-6ioLJgoKGM8oZe_0IXvg0vLJUoIVpQkF4miW8oEH2MAq9rQrHRYK0rUphK1rUSlStSmErVOmYuduZ-toN4nvjtIQL4FYhq5OYQfT_9r_QLpHJjd</recordid><startdate>20171201</startdate><enddate>20171201</enddate><creator>Gong, Chunying</creator><creator>Zeng, Wenhui</creator><creator>Zhang, Tianyang</creator><creator>Liu, Rongpeng</creator><creator>Ou, Yao</creator><creator>Ai, Junwen</creator><creator>Xiang, Zhonghuai</creator><creator>Xu, Hanfu</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9239-0362</orcidid></search><sort><creationdate>20171201</creationdate><title>Effects of transgenic overexpression of diapause hormone and diapause hormone receptor genes on non-diapause silkworm</title><author>Gong, Chunying ; Zeng, Wenhui ; Zhang, Tianyang ; Liu, Rongpeng ; Ou, Yao ; Ai, Junwen ; Xiang, Zhonghuai ; Xu, Hanfu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-7159f986d0596d685c15e4f6fc1cafab95c0d5a0299d007a57d93a9113a6be03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animal Genetics and Genomics</topic><topic>Arthropods</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedical Engineering/Biotechnology</topic><topic>Bombyx mori</topic><topic>Brief Communication</topic><topic>Developmental stages</topic><topic>Diapause</topic><topic>Embryos</topic><topic>Gene expression</topic><topic>Genetic Engineering</topic><topic>Hormones</topic><topic>Insects</topic><topic>Laboratories</topic><topic>Life Sciences</topic><topic>Molecular Medicine</topic><topic>Offspring</topic><topic>Ovaries</topic><topic>Plant Genetics and Genomics</topic><topic>Silkworms</topic><topic>Transgenics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gong, Chunying</creatorcontrib><creatorcontrib>Zeng, Wenhui</creatorcontrib><creatorcontrib>Zhang, Tianyang</creatorcontrib><creatorcontrib>Liu, Rongpeng</creatorcontrib><creatorcontrib>Ou, Yao</creatorcontrib><creatorcontrib>Ai, Junwen</creatorcontrib><creatorcontrib>Xiang, Zhonghuai</creatorcontrib><creatorcontrib>Xu, Hanfu</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Transgenic research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gong, Chunying</au><au>Zeng, Wenhui</au><au>Zhang, Tianyang</au><au>Liu, Rongpeng</au><au>Ou, Yao</au><au>Ai, Junwen</au><au>Xiang, Zhonghuai</au><au>Xu, Hanfu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of transgenic overexpression of diapause hormone and diapause hormone receptor genes on non-diapause silkworm</atitle><jtitle>Transgenic research</jtitle><stitle>Transgenic Res</stitle><addtitle>Transgenic Res</addtitle><date>2017-12-01</date><risdate>2017</risdate><volume>26</volume><issue>6</issue><spage>807</spage><epage>815</epage><pages>807-815</pages><issn>0962-8819</issn><eissn>1573-9368</eissn><abstract>Diapause is a state of developmental arrest that is most often observed in arthropods, especially insects. The domesticated silkworm,
Bombyx mori
, is a typical insect that enters diapause at an early embryonic stage. Previous studies have revealed that the diapause hormone (DH) signaling molecules, especially the core members DH and DH receptor 1 (DHR1), are crucial for the determination of embryonic diapause in diapause silkworm strains. However, whether they function in non-diapause silkworm strains remains largely unknown. Here, we generated two transgenic lines overexpressing
DH
or
DHR1
genes in a non-diapause silkworm strain,
Nistari
. Our results showed that developmental expression patterns of
DH
and
DHR1
are quite similar in transgenic silkworms: both genes are highly expressed in the mid to late stages of pupae and are most highly expressed in day-6 pupae but are expressed at very low levels in other developmental stages. Moreover, the overexpression of
DH
or
DHR1
can affect the expression of diapause-related genes but is not sufficient to induce embryonic diapause in their offspring. This study provides new insights into the function of DH and DHR1 in a non-diapause silkworm strain.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>28952064</pmid><doi>10.1007/s11248-017-0045-y</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-9239-0362</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0962-8819 |
| ispartof | Transgenic research, 2017-12, Vol.26 (6), p.807-815 |
| issn | 0962-8819 1573-9368 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1943641335 |
| source | Springer Nature - Complete Springer Journals |
| subjects | Animal Genetics and Genomics Arthropods Biomedical and Life Sciences Biomedical Engineering/Biotechnology Bombyx mori Brief Communication Developmental stages Diapause Embryos Gene expression Genetic Engineering Hormones Insects Laboratories Life Sciences Molecular Medicine Offspring Ovaries Plant Genetics and Genomics Silkworms Transgenics |
| title | Effects of transgenic overexpression of diapause hormone and diapause hormone receptor genes on non-diapause silkworm |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-22T08%3A20%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effects%20of%20transgenic%20overexpression%20of%20diapause%20hormone%20and%20diapause%20hormone%20receptor%20genes%20on%20non-diapause%20silkworm&rft.jtitle=Transgenic%20research&rft.au=Gong,%20Chunying&rft.date=2017-12-01&rft.volume=26&rft.issue=6&rft.spage=807&rft.epage=815&rft.pages=807-815&rft.issn=0962-8819&rft.eissn=1573-9368&rft_id=info:doi/10.1007/s11248-017-0045-y&rft_dat=%3Cproquest_cross%3E1943641335%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1966347026&rft_id=info:pmid/28952064&rfr_iscdi=true |