Identification and characterization of a rabbit novel IFN-α unlocated in genome
The multigene family of rabbit IFN-α (RbIFN-α) is located on chromosome 1, which shows seven functional genes in type I IFN locus. A novel RbIFN-α that remains unlocated in the rabbit genome was amplified and designated as the first novel rabbit IFN-α (RbIFN-αNov1), which possesses the typical molec...
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| Veröffentlicht in: | Developmental and comparative immunology 2018-01, Vol.78, p.91-99 |
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| container_title | Developmental and comparative immunology |
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| creator | Gao, Mingchun Liu, Ying Guo, Yongli Wang, Yujiao Dai, Haiyue Song, Zhifeng Wang, Junwei Han, Wenyu |
| description | The multigene family of rabbit IFN-α (RbIFN-α) is located on chromosome 1, which shows seven functional genes in type I IFN locus. A novel RbIFN-α that remains unlocated in the rabbit genome was amplified and designated as the first novel rabbit IFN-α (RbIFN-αNov1), which possesses the typical molecular characteristics of type I IFNs and could be induced in RK-13 cells and peripheral blood mononuclear cells. After the mature peptide of RbIFN-αNov1 was expressed, its antiviral activity, physicochemical characteristics, and cytotoxicity were determined in vitro. Results indicated that RbIFN-αNov1 exerted a high specific antiviral activity against VSV and a low cytotoxic effect on RK-13 cells. RbIFN-αNov1 showed high sensitivity to trypsin and remained relatively stable after acid, alkali, or heat treatment. RbIFN-αNov1 could induce Mx1 expression on RK-13 cells and activate the NF-κB, ISRE and BoIFN-β promoter activities on bovine testicular cells. Overall, our research on RbIFN-αNov1 not only enriches the knowledge about rabbit IFNs but also makes RbIFN-αNov1 have the potential to be used as an effective therapeutic agent for rabbit viral diseases.
•Rabbit type I interferon locus was constructed and analyzed.•An unlocated rabbit IFN-α designated as RbIFN-αNov1 was characterized.•RbIFN-αNov1 has broad antiviral activity and antiproliferative activity.•RbIFN-αNov1 can induce Mx1 expression to exert antiviral activity.•RbIFN-αNov1 can induce NF-κB, ISRE and BoIFNβ promotor activity on BT cells. |
| doi_str_mv | 10.1016/j.dci.2017.09.016 |
| format | Article |
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•Rabbit type I interferon locus was constructed and analyzed.•An unlocated rabbit IFN-α designated as RbIFN-αNov1 was characterized.•RbIFN-αNov1 has broad antiviral activity and antiproliferative activity.•RbIFN-αNov1 can induce Mx1 expression to exert antiviral activity.•RbIFN-αNov1 can induce NF-κB, ISRE and BoIFNβ promotor activity on BT cells.</description><identifier>ISSN: 0145-305X</identifier><identifier>EISSN: 1879-0089</identifier><identifier>DOI: 10.1016/j.dci.2017.09.016</identifier><identifier>PMID: 28942155</identifier><language>eng</language><publisher>United States: Elsevier Ltd</publisher><subject>Animals ; Antiviral Agents - metabolism ; Biological activity ; Cell Line ; Cell Survival ; Cloning, Molecular ; Epithelial Cells - physiology ; Epithelial Cells - virology ; Expression distribution ; Gene Expression Regulation ; Interferon-alpha - genetics ; Interferon-alpha - metabolism ; Interferon-beta - genetics ; Interferon-beta - metabolism ; Leukocytes, Mononuclear - physiology ; Molecular characteristics ; Myxovirus Resistance Proteins - genetics ; Myxovirus Resistance Proteins - metabolism ; NF-kappa B - metabolism ; Promoter Regions, Genetic - genetics ; Protein Stability ; Rabbit interferon-α ; Rabbits ; Rhabdoviridae Infections - immunology ; Trypsin - metabolism ; Type I IFN locus ; Vesicular stomatitis Indiana virus - immunology</subject><ispartof>Developmental and comparative immunology, 2018-01, Vol.78, p.91-99</ispartof><rights>2017 Elsevier Ltd</rights><rights>Copyright © 2017 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-123f905175749e1cc60236feb2547cb36a26f49e32df9c57a17c3e30845934073</citedby><cites>FETCH-LOGICAL-c353t-123f905175749e1cc60236feb2547cb36a26f49e32df9c57a17c3e30845934073</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.dci.2017.09.016$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28942155$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gao, Mingchun</creatorcontrib><creatorcontrib>Liu, Ying</creatorcontrib><creatorcontrib>Guo, Yongli</creatorcontrib><creatorcontrib>Wang, Yujiao</creatorcontrib><creatorcontrib>Dai, Haiyue</creatorcontrib><creatorcontrib>Song, Zhifeng</creatorcontrib><creatorcontrib>Wang, Junwei</creatorcontrib><creatorcontrib>Han, Wenyu</creatorcontrib><title>Identification and characterization of a rabbit novel IFN-α unlocated in genome</title><title>Developmental and comparative immunology</title><addtitle>Dev Comp Immunol</addtitle><description>The multigene family of rabbit IFN-α (RbIFN-α) is located on chromosome 1, which shows seven functional genes in type I IFN locus. A novel RbIFN-α that remains unlocated in the rabbit genome was amplified and designated as the first novel rabbit IFN-α (RbIFN-αNov1), which possesses the typical molecular characteristics of type I IFNs and could be induced in RK-13 cells and peripheral blood mononuclear cells. After the mature peptide of RbIFN-αNov1 was expressed, its antiviral activity, physicochemical characteristics, and cytotoxicity were determined in vitro. Results indicated that RbIFN-αNov1 exerted a high specific antiviral activity against VSV and a low cytotoxic effect on RK-13 cells. RbIFN-αNov1 showed high sensitivity to trypsin and remained relatively stable after acid, alkali, or heat treatment. RbIFN-αNov1 could induce Mx1 expression on RK-13 cells and activate the NF-κB, ISRE and BoIFN-β promoter activities on bovine testicular cells. Overall, our research on RbIFN-αNov1 not only enriches the knowledge about rabbit IFNs but also makes RbIFN-αNov1 have the potential to be used as an effective therapeutic agent for rabbit viral diseases.
•Rabbit type I interferon locus was constructed and analyzed.•An unlocated rabbit IFN-α designated as RbIFN-αNov1 was characterized.•RbIFN-αNov1 has broad antiviral activity and antiproliferative activity.•RbIFN-αNov1 can induce Mx1 expression to exert antiviral activity.•RbIFN-αNov1 can induce NF-κB, ISRE and BoIFNβ promotor activity on BT cells.</description><subject>Animals</subject><subject>Antiviral Agents - metabolism</subject><subject>Biological activity</subject><subject>Cell Line</subject><subject>Cell Survival</subject><subject>Cloning, Molecular</subject><subject>Epithelial Cells - physiology</subject><subject>Epithelial Cells - virology</subject><subject>Expression distribution</subject><subject>Gene Expression Regulation</subject><subject>Interferon-alpha - genetics</subject><subject>Interferon-alpha - metabolism</subject><subject>Interferon-beta - genetics</subject><subject>Interferon-beta - metabolism</subject><subject>Leukocytes, Mononuclear - physiology</subject><subject>Molecular characteristics</subject><subject>Myxovirus Resistance Proteins - genetics</subject><subject>Myxovirus Resistance Proteins - metabolism</subject><subject>NF-kappa B - metabolism</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Protein Stability</subject><subject>Rabbit interferon-α</subject><subject>Rabbits</subject><subject>Rhabdoviridae Infections - immunology</subject><subject>Trypsin - metabolism</subject><subject>Type I IFN locus</subject><subject>Vesicular stomatitis Indiana virus - immunology</subject><issn>0145-305X</issn><issn>1879-0089</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1KLDEQhYNc0fHnAdxIlnfTbaXT6XRwdRF_BkRdKLgL6XS1ZuhJNOkR9K18EZ_JyOhdWpuCU-ccqI-QAwYlA9YcLcreurICJktQZVY2yIy1UhUArfpDZsBqUXAQ99tkJ6UF5GkZbJHtqlV1xYSYkZt5j35yg7NmcsFT43tqH000dsLo3tZiGKih0XSdm6gPLzjS-dlV8fFOV34MOYg9dZ4-oA9L3CObgxkT7n_vXXJ3dnp7clFcXp_PT_5dFpYLPhWs4oMCwaSQtUJmbQMVbwbsKlFL2_HGVM2QL7zqB2WFNExajhzaWiheg-S75O-69ymG5xWmSS9dsjiOxmNYJc3yh7KGuoFsZWurjSGliIN-im5p4qtmoL9A6oXOIPUXSA1KZyVnDr_rV90S-_-JH3LZcLw2YH7yxWHUyTr0FnsX0U66D-6X-k8g7IKG</recordid><startdate>201801</startdate><enddate>201801</enddate><creator>Gao, Mingchun</creator><creator>Liu, Ying</creator><creator>Guo, Yongli</creator><creator>Wang, Yujiao</creator><creator>Dai, Haiyue</creator><creator>Song, Zhifeng</creator><creator>Wang, Junwei</creator><creator>Han, Wenyu</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201801</creationdate><title>Identification and characterization of a rabbit novel IFN-α unlocated in genome</title><author>Gao, Mingchun ; Liu, Ying ; Guo, Yongli ; Wang, Yujiao ; Dai, Haiyue ; Song, Zhifeng ; Wang, Junwei ; Han, Wenyu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-123f905175749e1cc60236feb2547cb36a26f49e32df9c57a17c3e30845934073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Antiviral Agents - metabolism</topic><topic>Biological activity</topic><topic>Cell Line</topic><topic>Cell Survival</topic><topic>Cloning, Molecular</topic><topic>Epithelial Cells - physiology</topic><topic>Epithelial Cells - virology</topic><topic>Expression distribution</topic><topic>Gene Expression Regulation</topic><topic>Interferon-alpha - genetics</topic><topic>Interferon-alpha - metabolism</topic><topic>Interferon-beta - genetics</topic><topic>Interferon-beta - metabolism</topic><topic>Leukocytes, Mononuclear - physiology</topic><topic>Molecular characteristics</topic><topic>Myxovirus Resistance Proteins - genetics</topic><topic>Myxovirus Resistance Proteins - metabolism</topic><topic>NF-kappa B - metabolism</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Protein Stability</topic><topic>Rabbit interferon-α</topic><topic>Rabbits</topic><topic>Rhabdoviridae Infections - immunology</topic><topic>Trypsin - metabolism</topic><topic>Type I IFN locus</topic><topic>Vesicular stomatitis Indiana virus - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gao, Mingchun</creatorcontrib><creatorcontrib>Liu, Ying</creatorcontrib><creatorcontrib>Guo, Yongli</creatorcontrib><creatorcontrib>Wang, Yujiao</creatorcontrib><creatorcontrib>Dai, Haiyue</creatorcontrib><creatorcontrib>Song, Zhifeng</creatorcontrib><creatorcontrib>Wang, Junwei</creatorcontrib><creatorcontrib>Han, Wenyu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental and comparative immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gao, Mingchun</au><au>Liu, Ying</au><au>Guo, Yongli</au><au>Wang, Yujiao</au><au>Dai, Haiyue</au><au>Song, Zhifeng</au><au>Wang, Junwei</au><au>Han, Wenyu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and characterization of a rabbit novel IFN-α unlocated in genome</atitle><jtitle>Developmental and comparative immunology</jtitle><addtitle>Dev Comp Immunol</addtitle><date>2018-01</date><risdate>2018</risdate><volume>78</volume><spage>91</spage><epage>99</epage><pages>91-99</pages><issn>0145-305X</issn><eissn>1879-0089</eissn><abstract>The multigene family of rabbit IFN-α (RbIFN-α) is located on chromosome 1, which shows seven functional genes in type I IFN locus. A novel RbIFN-α that remains unlocated in the rabbit genome was amplified and designated as the first novel rabbit IFN-α (RbIFN-αNov1), which possesses the typical molecular characteristics of type I IFNs and could be induced in RK-13 cells and peripheral blood mononuclear cells. After the mature peptide of RbIFN-αNov1 was expressed, its antiviral activity, physicochemical characteristics, and cytotoxicity were determined in vitro. Results indicated that RbIFN-αNov1 exerted a high specific antiviral activity against VSV and a low cytotoxic effect on RK-13 cells. RbIFN-αNov1 showed high sensitivity to trypsin and remained relatively stable after acid, alkali, or heat treatment. RbIFN-αNov1 could induce Mx1 expression on RK-13 cells and activate the NF-κB, ISRE and BoIFN-β promoter activities on bovine testicular cells. Overall, our research on RbIFN-αNov1 not only enriches the knowledge about rabbit IFNs but also makes RbIFN-αNov1 have the potential to be used as an effective therapeutic agent for rabbit viral diseases.
•Rabbit type I interferon locus was constructed and analyzed.•An unlocated rabbit IFN-α designated as RbIFN-αNov1 was characterized.•RbIFN-αNov1 has broad antiviral activity and antiproliferative activity.•RbIFN-αNov1 can induce Mx1 expression to exert antiviral activity.•RbIFN-αNov1 can induce NF-κB, ISRE and BoIFNβ promotor activity on BT cells.</abstract><cop>United States</cop><pub>Elsevier Ltd</pub><pmid>28942155</pmid><doi>10.1016/j.dci.2017.09.016</doi><tpages>9</tpages></addata></record> |
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| subjects | Animals Antiviral Agents - metabolism Biological activity Cell Line Cell Survival Cloning, Molecular Epithelial Cells - physiology Epithelial Cells - virology Expression distribution Gene Expression Regulation Interferon-alpha - genetics Interferon-alpha - metabolism Interferon-beta - genetics Interferon-beta - metabolism Leukocytes, Mononuclear - physiology Molecular characteristics Myxovirus Resistance Proteins - genetics Myxovirus Resistance Proteins - metabolism NF-kappa B - metabolism Promoter Regions, Genetic - genetics Protein Stability Rabbit interferon-α Rabbits Rhabdoviridae Infections - immunology Trypsin - metabolism Type I IFN locus Vesicular stomatitis Indiana virus - immunology |
| title | Identification and characterization of a rabbit novel IFN-α unlocated in genome |
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