Satellite cell activation and mTOR signaling pathway response to resistance and combined exercise in elite weight lifters
Purpose Our aim was to compare the effects of a single exercise training mode (resistance exercise) with a combined exercise training (resistance and plyometric exercise) mode on satellite cell activity and anabolic signaling at the molecular level. Methods Eighteen male weight lifters (20 ± 4 years...
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| Veröffentlicht in: | European journal of applied physiology 2017-11, Vol.117 (11), p.2355-2363 |
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| creator | Lim, Chang Hyun Luu, Thien Suong Phoung, Le Quy Jeong, Tae Seok Kim, Chang Keun |
| description | Purpose
Our aim was to compare the effects of a single exercise training mode (resistance exercise) with a combined exercise training (resistance and plyometric exercise) mode on satellite cell activity and anabolic signaling at the molecular level.
Methods
Eighteen male weight lifters (20 ± 4 years, BMI 27 ± 6 kg/m
2
) were randomly assigned to either a series of resistance exercise or a series of combined exercise group. The intensity of the exercise was set at 60% of their 1 RM weight and subjects completed three sets each of six repetitions. The combined exercise group performed three different types of resistance exercise alternating with three different types of plyometric exercise, whereas the resistance exercise group performed only the three different types of resistance exercise which was repeated twice. Muscle biopsies were obtained the vastus lateralis muscle immediately before and 3 h after one bout of exercise.
Results
Exercise induced increases in satellite cell activation and myofibrillar protein synthesis following both exercise modes, but the resistance exercise group was superior compared to the combined exercise group in satellite cell activity expressed by Ki67/CD56 (165 vs 232%) and PI3K/Akt protein expression (121 vs 157%), mTOR protein expression (117 vs 288%), p70S6K protein expression (253 vs 809%), and 4E-BP1 protein expression (70 vs 139%) of anabolic signaling pathway.
Conclusions
These results suggest that the previous findings showing a greater effect of combined as opposed to a single exercise mode could be the effect of a greater training volume rather than a true-training effect of a combined exercise program. |
| doi_str_mv | 10.1007/s00421-017-3722-x |
| format | Article |
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Our aim was to compare the effects of a single exercise training mode (resistance exercise) with a combined exercise training (resistance and plyometric exercise) mode on satellite cell activity and anabolic signaling at the molecular level.
Methods
Eighteen male weight lifters (20 ± 4 years, BMI 27 ± 6 kg/m
2
) were randomly assigned to either a series of resistance exercise or a series of combined exercise group. The intensity of the exercise was set at 60% of their 1 RM weight and subjects completed three sets each of six repetitions. The combined exercise group performed three different types of resistance exercise alternating with three different types of plyometric exercise, whereas the resistance exercise group performed only the three different types of resistance exercise which was repeated twice. Muscle biopsies were obtained the vastus lateralis muscle immediately before and 3 h after one bout of exercise.
Results
Exercise induced increases in satellite cell activation and myofibrillar protein synthesis following both exercise modes, but the resistance exercise group was superior compared to the combined exercise group in satellite cell activity expressed by Ki67/CD56 (165 vs 232%) and PI3K/Akt protein expression (121 vs 157%), mTOR protein expression (117 vs 288%), p70S6K protein expression (253 vs 809%), and 4E-BP1 protein expression (70 vs 139%) of anabolic signaling pathway.
Conclusions
These results suggest that the previous findings showing a greater effect of combined as opposed to a single exercise mode could be the effect of a greater training volume rather than a true-training effect of a combined exercise program.</description><identifier>ISSN: 1439-6319</identifier><identifier>EISSN: 1439-6327</identifier><identifier>DOI: 10.1007/s00421-017-3722-x</identifier><identifier>PMID: 28940037</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>1-Phosphatidylinositol 3-kinase ; Adolescent ; AKT protein ; Biomedical and Life Sciences ; Biomedicine ; CD56 antigen ; Cell activation ; Fitness training programs ; Human Physiology ; Humans ; Male ; Occupational Medicine/Industrial Medicine ; Original Article ; Plyometric Exercise - adverse effects ; Plyometric Exercise - methods ; Protein biosynthesis ; Protein expression ; Proteins ; Quadriceps Muscle - metabolism ; Quadriceps Muscle - physiology ; Resistance Training - adverse effects ; Resistance Training - methods ; Satellite Cells, Skeletal Muscle - metabolism ; Signal Transduction ; Sports Medicine ; TOR protein ; TOR Serine-Threonine Kinases - metabolism ; Weight Lifting - physiology ; Young Adult</subject><ispartof>European journal of applied physiology, 2017-11, Vol.117 (11), p.2355-2363</ispartof><rights>Springer-Verlag GmbH Germany 2017</rights><rights>European Journal of Applied Physiology is a copyright of Springer, 2017.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c491t-d38d42bd1ad230cbdd98509ecffce19582ab331ac8ab7bf1c9df3cf0c9c92a573</citedby><cites>FETCH-LOGICAL-c491t-d38d42bd1ad230cbdd98509ecffce19582ab331ac8ab7bf1c9df3cf0c9c92a573</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00421-017-3722-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00421-017-3722-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,41493,42562,51324</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28940037$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lim, Chang Hyun</creatorcontrib><creatorcontrib>Luu, Thien Suong</creatorcontrib><creatorcontrib>Phoung, Le Quy</creatorcontrib><creatorcontrib>Jeong, Tae Seok</creatorcontrib><creatorcontrib>Kim, Chang Keun</creatorcontrib><title>Satellite cell activation and mTOR signaling pathway response to resistance and combined exercise in elite weight lifters</title><title>European journal of applied physiology</title><addtitle>Eur J Appl Physiol</addtitle><addtitle>Eur J Appl Physiol</addtitle><description>Purpose
Our aim was to compare the effects of a single exercise training mode (resistance exercise) with a combined exercise training (resistance and plyometric exercise) mode on satellite cell activity and anabolic signaling at the molecular level.
Methods
Eighteen male weight lifters (20 ± 4 years, BMI 27 ± 6 kg/m
2
) were randomly assigned to either a series of resistance exercise or a series of combined exercise group. The intensity of the exercise was set at 60% of their 1 RM weight and subjects completed three sets each of six repetitions. The combined exercise group performed three different types of resistance exercise alternating with three different types of plyometric exercise, whereas the resistance exercise group performed only the three different types of resistance exercise which was repeated twice. Muscle biopsies were obtained the vastus lateralis muscle immediately before and 3 h after one bout of exercise.
Results
Exercise induced increases in satellite cell activation and myofibrillar protein synthesis following both exercise modes, but the resistance exercise group was superior compared to the combined exercise group in satellite cell activity expressed by Ki67/CD56 (165 vs 232%) and PI3K/Akt protein expression (121 vs 157%), mTOR protein expression (117 vs 288%), p70S6K protein expression (253 vs 809%), and 4E-BP1 protein expression (70 vs 139%) of anabolic signaling pathway.
Conclusions
These results suggest that the previous findings showing a greater effect of combined as opposed to a single exercise mode could be the effect of a greater training volume rather than a true-training effect of a combined exercise program.</description><subject>1-Phosphatidylinositol 3-kinase</subject><subject>Adolescent</subject><subject>AKT protein</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>CD56 antigen</subject><subject>Cell activation</subject><subject>Fitness training programs</subject><subject>Human Physiology</subject><subject>Humans</subject><subject>Male</subject><subject>Occupational Medicine/Industrial Medicine</subject><subject>Original Article</subject><subject>Plyometric Exercise - adverse effects</subject><subject>Plyometric Exercise - methods</subject><subject>Protein biosynthesis</subject><subject>Protein expression</subject><subject>Proteins</subject><subject>Quadriceps Muscle - metabolism</subject><subject>Quadriceps Muscle - physiology</subject><subject>Resistance Training - adverse effects</subject><subject>Resistance Training - methods</subject><subject>Satellite Cells, Skeletal Muscle - metabolism</subject><subject>Signal Transduction</subject><subject>Sports Medicine</subject><subject>TOR protein</subject><subject>TOR Serine-Threonine Kinases - metabolism</subject><subject>Weight Lifting - physiology</subject><subject>Young Adult</subject><issn>1439-6319</issn><issn>1439-6327</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kc1r3DAQxUVpaT7aP6CXIuilF7czkndtHUPIFwQCbXoWsjTeKNjyRtImu_995WwaSqGnGZjfe8PjMfYJ4RsCNN8TQC2wAmwq2QhRbd-wQ6ylqpZSNG9fd1QH7CilewBoBbbv2YFoVQ0gm0O2-2kyDYPPxG2Z3NjsH032U-AmOD7e3vzgya-CGXxY8bXJd09mxyOl9RQS8TzNu0_ZBEvPCjuNnQ_kOG0pWl8YHzg9P3giv7rLfPB9ppg-sHe9GRJ9fJnH7Nf52e3pZXV9c3F1enJd2VphrpxsXS06h8YJCbZzTrULUGT73hKqRStMJyUa25qu6Xq0yvXS9mCVVcIsGnnMvu5913F62FDKevRpzmoCTZukUdWigWW7lAX98g96P21iyT5TCxQoQUChcE_ZOKUUqdfr6EcTdxpBz73ofS-69KLnXvS2aD6_OG-6kdyr4k8RBRB7IJVTWFH86_V_XX8Dt5mbYQ</recordid><startdate>20171101</startdate><enddate>20171101</enddate><creator>Lim, Chang Hyun</creator><creator>Luu, Thien Suong</creator><creator>Phoung, Le Quy</creator><creator>Jeong, Tae Seok</creator><creator>Kim, Chang Keun</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20171101</creationdate><title>Satellite cell activation and mTOR signaling pathway response to resistance and combined exercise in elite weight lifters</title><author>Lim, Chang Hyun ; Luu, Thien Suong ; Phoung, Le Quy ; Jeong, Tae Seok ; Kim, Chang Keun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c491t-d38d42bd1ad230cbdd98509ecffce19582ab331ac8ab7bf1c9df3cf0c9c92a573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>1-Phosphatidylinositol 3-kinase</topic><topic>Adolescent</topic><topic>AKT protein</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>CD56 antigen</topic><topic>Cell activation</topic><topic>Fitness training programs</topic><topic>Human Physiology</topic><topic>Humans</topic><topic>Male</topic><topic>Occupational Medicine/Industrial Medicine</topic><topic>Original Article</topic><topic>Plyometric Exercise - adverse effects</topic><topic>Plyometric Exercise - methods</topic><topic>Protein biosynthesis</topic><topic>Protein expression</topic><topic>Proteins</topic><topic>Quadriceps Muscle - metabolism</topic><topic>Quadriceps Muscle - physiology</topic><topic>Resistance Training - adverse effects</topic><topic>Resistance Training - methods</topic><topic>Satellite Cells, Skeletal Muscle - metabolism</topic><topic>Signal Transduction</topic><topic>Sports Medicine</topic><topic>TOR protein</topic><topic>TOR Serine-Threonine Kinases - metabolism</topic><topic>Weight Lifting - physiology</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lim, Chang Hyun</creatorcontrib><creatorcontrib>Luu, Thien Suong</creatorcontrib><creatorcontrib>Phoung, Le Quy</creatorcontrib><creatorcontrib>Jeong, Tae Seok</creatorcontrib><creatorcontrib>Kim, Chang Keun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of applied physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lim, Chang Hyun</au><au>Luu, Thien Suong</au><au>Phoung, Le Quy</au><au>Jeong, Tae Seok</au><au>Kim, Chang Keun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Satellite cell activation and mTOR signaling pathway response to resistance and combined exercise in elite weight lifters</atitle><jtitle>European journal of applied physiology</jtitle><stitle>Eur J Appl Physiol</stitle><addtitle>Eur J Appl Physiol</addtitle><date>2017-11-01</date><risdate>2017</risdate><volume>117</volume><issue>11</issue><spage>2355</spage><epage>2363</epage><pages>2355-2363</pages><issn>1439-6319</issn><eissn>1439-6327</eissn><abstract>Purpose
Our aim was to compare the effects of a single exercise training mode (resistance exercise) with a combined exercise training (resistance and plyometric exercise) mode on satellite cell activity and anabolic signaling at the molecular level.
Methods
Eighteen male weight lifters (20 ± 4 years, BMI 27 ± 6 kg/m
2
) were randomly assigned to either a series of resistance exercise or a series of combined exercise group. The intensity of the exercise was set at 60% of their 1 RM weight and subjects completed three sets each of six repetitions. The combined exercise group performed three different types of resistance exercise alternating with three different types of plyometric exercise, whereas the resistance exercise group performed only the three different types of resistance exercise which was repeated twice. Muscle biopsies were obtained the vastus lateralis muscle immediately before and 3 h after one bout of exercise.
Results
Exercise induced increases in satellite cell activation and myofibrillar protein synthesis following both exercise modes, but the resistance exercise group was superior compared to the combined exercise group in satellite cell activity expressed by Ki67/CD56 (165 vs 232%) and PI3K/Akt protein expression (121 vs 157%), mTOR protein expression (117 vs 288%), p70S6K protein expression (253 vs 809%), and 4E-BP1 protein expression (70 vs 139%) of anabolic signaling pathway.
Conclusions
These results suggest that the previous findings showing a greater effect of combined as opposed to a single exercise mode could be the effect of a greater training volume rather than a true-training effect of a combined exercise program.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>28940037</pmid><doi>10.1007/s00421-017-3722-x</doi><tpages>9</tpages></addata></record> |
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| ispartof | European journal of applied physiology, 2017-11, Vol.117 (11), p.2355-2363 |
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| subjects | 1-Phosphatidylinositol 3-kinase Adolescent AKT protein Biomedical and Life Sciences Biomedicine CD56 antigen Cell activation Fitness training programs Human Physiology Humans Male Occupational Medicine/Industrial Medicine Original Article Plyometric Exercise - adverse effects Plyometric Exercise - methods Protein biosynthesis Protein expression Proteins Quadriceps Muscle - metabolism Quadriceps Muscle - physiology Resistance Training - adverse effects Resistance Training - methods Satellite Cells, Skeletal Muscle - metabolism Signal Transduction Sports Medicine TOR protein TOR Serine-Threonine Kinases - metabolism Weight Lifting - physiology Young Adult |
| title | Satellite cell activation and mTOR signaling pathway response to resistance and combined exercise in elite weight lifters |
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