Profiling of Sugar Nucleotides
Sugar nucleotides are essential building blocks for the glycobiology of all living organisms. Detailed information on the types of sugar nucleotides present in a particular cell and how they change as a function of metabolic, developmental, or disease status is vital. The extraction, identification,...
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| Veröffentlicht in: | Methods in enzymology 2017, Vol.597, p.209-238 |
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| container_title | Methods in enzymology |
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| creator | Rejzek, Martin Hill, Lionel Hems, Edward S Kuhaudomlarp, Sakonwan Wagstaff, Ben A Field, Robert A |
| description | Sugar nucleotides are essential building blocks for the glycobiology of all living organisms. Detailed information on the types of sugar nucleotides present in a particular cell and how they change as a function of metabolic, developmental, or disease status is vital. The extraction, identification, and quantification of sugar nucleotides in a given sample present formidable challenges. In this chapter, currently used techniques for sugar nucleotide extraction from cells, separation from complex biological matrices, and detection by optical and mass spectrometry methods are discussed. |
| doi_str_mv | 10.1016/bs.mie.2017.06.005 |
| format | Article |
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Detailed information on the types of sugar nucleotides present in a particular cell and how they change as a function of metabolic, developmental, or disease status is vital. The extraction, identification, and quantification of sugar nucleotides in a given sample present formidable challenges. In this chapter, currently used techniques for sugar nucleotide extraction from cells, separation from complex biological matrices, and detection by optical and mass spectrometry methods are discussed.</description><identifier>EISSN: 1557-7988</identifier><identifier>DOI: 10.1016/bs.mie.2017.06.005</identifier><identifier>PMID: 28935103</identifier><language>eng</language><publisher>United States</publisher><subject>Chromatography, High Pressure Liquid - methods ; Glycomics - methods ; Humans ; Nucleotides - chemistry ; Nucleotides - isolation & purification ; Nucleotides - metabolism ; Sugars - chemistry ; Sugars - isolation & purification ; Sugars - metabolism ; Tandem Mass Spectrometry - methods</subject><ispartof>Methods in enzymology, 2017, Vol.597, p.209-238</ispartof><rights>2017 Elsevier Inc. 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Detailed information on the types of sugar nucleotides present in a particular cell and how they change as a function of metabolic, developmental, or disease status is vital. The extraction, identification, and quantification of sugar nucleotides in a given sample present formidable challenges. 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| fulltext | fulltext |
| identifier | EISSN: 1557-7988 |
| ispartof | Methods in enzymology, 2017, Vol.597, p.209-238 |
| issn | 1557-7988 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Chromatography, High Pressure Liquid - methods Glycomics - methods Humans Nucleotides - chemistry Nucleotides - isolation & purification Nucleotides - metabolism Sugars - chemistry Sugars - isolation & purification Sugars - metabolism Tandem Mass Spectrometry - methods |
| title | Profiling of Sugar Nucleotides |
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