Transient and stable vector transfection: Pitfalls, off-target effects, artifacts

[Display omitted] Transient and stable vector transfections have played important roles in illustrating the function of specific genes/proteins. The general assumption is that such a platform could effectively link a given gene/protein to gained phenotypes, revealing the mechanism of how a gene work...

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Veröffentlicht in:	Mutation research 2017-07, Vol.773, p.91-103
Hauptverfasser:	Stepanenko, Aleksei A., Heng, Henry H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Aneuploidy Animals Anti-Bacterial Agents - pharmacology Chromosomal instability Chromosomal Instability - genetics Cloning, Molecular Drug Resistance, Microbial - genetics Epigenomics Gene Dosage Gene Expression Regulation Genes, Reporter Genetic Vectors Geneticin/G418 Humans Plasmids - genetics Plasmids - metabolism Puromycin Transfection Transgenes Tumor heterogeneity Zeocin
Online-Zugang:	Volltext
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