Structure and chain conformation of a (1 → 6)-α- d-glucan from the root of Pueraria lobata (Willd.) Ohwi and the antioxidant activity of its sulfated derivative
A water soluble glucan, PLB-2C, was isolated from the water extract of the root of Pueraria lobata (Willd) Ohwi using anion-exchange and gel permeation chromatography. Its structure was investigated by gas chromatography (GC), gas chromatography–mass spectrometry (GC–MS), infrared (IR) spectra, and...
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| Veröffentlicht in: | Carbohydrate polymers 2008-11, Vol.74 (4), p.771-778 |
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| creator | Cui, Hengxiang Liu, Qin Tao, Yongzhen Zhang, Hongfeng Zhang, Lina Ding, Kan |
| description | A water soluble glucan, PLB-2C, was isolated from the water extract of the root of
Pueraria lobata (Willd) Ohwi using anion-exchange and gel permeation chromatography. Its structure was investigated by gas chromatography (GC), gas chromatography–mass spectrometry (GC–MS), infrared (IR) spectra, and nuclear magnetic resonance (NMR) spectroscopy of heteronuclear single quantum coherence (HSQC) and heteronuclear multiple bond correlation (HMBC) techniques. The results indicated that PLB-2C was a linear glucan composed of (1
→
6)-α-
d-Glc
p. Chain conformation study showed that the polysaccharide took random coil compact conformation.
In vitro cell viability assay by MTT method, its sulfated derivative PLB-2CS which was substituted at 2-O, 3-O, 4-O positions, at 0.1, 1, and 5
mg/ml, could attenuate PC12 cell damage significantly caused by hydrogen peroxide. |
| doi_str_mv | 10.1016/j.carbpol.2008.04.034 |
| format | Article |
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Pueraria lobata (Willd) Ohwi using anion-exchange and gel permeation chromatography. Its structure was investigated by gas chromatography (GC), gas chromatography–mass spectrometry (GC–MS), infrared (IR) spectra, and nuclear magnetic resonance (NMR) spectroscopy of heteronuclear single quantum coherence (HSQC) and heteronuclear multiple bond correlation (HMBC) techniques. The results indicated that PLB-2C was a linear glucan composed of (1
→
6)-α-
d-Glc
p. Chain conformation study showed that the polysaccharide took random coil compact conformation.
In vitro cell viability assay by MTT method, its sulfated derivative PLB-2CS which was substituted at 2-O, 3-O, 4-O positions, at 0.1, 1, and 5
mg/ml, could attenuate PC12 cell damage significantly caused by hydrogen peroxide.</description><identifier>ISSN: 0144-8617</identifier><identifier>EISSN: 1879-1344</identifier><identifier>DOI: 10.1016/j.carbpol.2008.04.034</identifier><identifier>CODEN: CAPOD8</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>(1 → 6)-α- d-Glucan ; antioxidant activity ; Antioxidant effect ; Applied sciences ; Biological and medical sciences ; biopolymers ; Chain conformation ; chemical composition ; Chemical constitution ; chemical structure ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; glucans ; herbal medicines ; Natural polymers ; Physicochemistry of polymers ; plant extracts ; Plant physiology and development ; Pueraria lobata ; Pueraria montana var. lobata ; roots ; Starch and polysaccharides ; sulfate derivatives ; Sulfated polysaccharide ; sulfates</subject><ispartof>Carbohydrate polymers, 2008-11, Vol.74 (4), p.771-778</ispartof><rights>2008 Elsevier Ltd</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c394t-81224ba3a8c1510d74d5bdf9e9c023180abe10016f384f92f980a477a9ec4a0f3</citedby><cites>FETCH-LOGICAL-c394t-81224ba3a8c1510d74d5bdf9e9c023180abe10016f384f92f980a477a9ec4a0f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0144861708002117$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20656722$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Cui, Hengxiang</creatorcontrib><creatorcontrib>Liu, Qin</creatorcontrib><creatorcontrib>Tao, Yongzhen</creatorcontrib><creatorcontrib>Zhang, Hongfeng</creatorcontrib><creatorcontrib>Zhang, Lina</creatorcontrib><creatorcontrib>Ding, Kan</creatorcontrib><title>Structure and chain conformation of a (1 → 6)-α- d-glucan from the root of Pueraria lobata (Willd.) Ohwi and the antioxidant activity of its sulfated derivative</title><title>Carbohydrate polymers</title><description>A water soluble glucan, PLB-2C, was isolated from the water extract of the root of
Pueraria lobata (Willd) Ohwi using anion-exchange and gel permeation chromatography. Its structure was investigated by gas chromatography (GC), gas chromatography–mass spectrometry (GC–MS), infrared (IR) spectra, and nuclear magnetic resonance (NMR) spectroscopy of heteronuclear single quantum coherence (HSQC) and heteronuclear multiple bond correlation (HMBC) techniques. The results indicated that PLB-2C was a linear glucan composed of (1
→
6)-α-
d-Glc
p. Chain conformation study showed that the polysaccharide took random coil compact conformation.
In vitro cell viability assay by MTT method, its sulfated derivative PLB-2CS which was substituted at 2-O, 3-O, 4-O positions, at 0.1, 1, and 5
mg/ml, could attenuate PC12 cell damage significantly caused by hydrogen peroxide.</description><subject>(1 → 6)-α- d-Glucan</subject><subject>antioxidant activity</subject><subject>Antioxidant effect</subject><subject>Applied sciences</subject><subject>Biological and medical sciences</subject><subject>biopolymers</subject><subject>Chain conformation</subject><subject>chemical composition</subject><subject>Chemical constitution</subject><subject>chemical structure</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>glucans</subject><subject>herbal medicines</subject><subject>Natural polymers</subject><subject>Physicochemistry of polymers</subject><subject>plant extracts</subject><subject>Plant physiology and development</subject><subject>Pueraria lobata</subject><subject>Pueraria montana var. lobata</subject><subject>roots</subject><subject>Starch and polysaccharides</subject><subject>sulfate derivatives</subject><subject>Sulfated polysaccharide</subject><subject>sulfates</subject><issn>0144-8617</issn><issn>1879-1344</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNqFkUtuFDEQhlsIJIbAERDegMiiG9vtfniFUBQeUqQghYilVe1HxiOPPdjugVwgB-AKnICLcAhOgpsZsaU2JZW-v6r0_1X1lOCGYNK_2jQS4rQLrqEYjw1mDW7ZvWpFxoHXpGXsfrXChLF67MnwsHqU0gaX6gleVT-ucpxlnqNG4BWSa7AeyeBNiFvINngUDAL0kqDfd99Rf1r_-lkjVd-4WYJHJoYtymuNYgh5IT_OOkK0gFyYIBfdZ-ucak7R5fqr_XthocGXzd-sKh2BzHZv8-2itjmhNDsDWSukdLT78sJeP64eGHBJPzn2k-r67fmns_f1xeW7D2dvLmrZcpbrkVDKJmhhlKQjWA1MdZMyXHOJaUtGDJMmuBhm2pEZTg0vIzYMwLVkgE17Ur047N3F8GXWKYutTVI7B16HOQnCWTGtHQvYHUAZQ0pRG7GLdgvxVhAslkjERhwjEUskAjNRIim658cDkCQ4E8FLm_6JKe67fqC0cM8OnIEg4CYW5vqKYtJi0nWEU16I1wdCFz_2VkeRpNVeamWjllmoYP_zyx-lxq_L</recordid><startdate>20081121</startdate><enddate>20081121</enddate><creator>Cui, Hengxiang</creator><creator>Liu, Qin</creator><creator>Tao, Yongzhen</creator><creator>Zhang, Hongfeng</creator><creator>Zhang, Lina</creator><creator>Ding, Kan</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20081121</creationdate><title>Structure and chain conformation of a (1 → 6)-α- d-glucan from the root of Pueraria lobata (Willd.) Ohwi and the antioxidant activity of its sulfated derivative</title><author>Cui, Hengxiang ; Liu, Qin ; Tao, Yongzhen ; Zhang, Hongfeng ; Zhang, Lina ; Ding, Kan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c394t-81224ba3a8c1510d74d5bdf9e9c023180abe10016f384f92f980a477a9ec4a0f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>(1 → 6)-α- d-Glucan</topic><topic>antioxidant activity</topic><topic>Antioxidant effect</topic><topic>Applied sciences</topic><topic>Biological and medical sciences</topic><topic>biopolymers</topic><topic>Chain conformation</topic><topic>chemical composition</topic><topic>Chemical constitution</topic><topic>chemical structure</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>glucans</topic><topic>herbal medicines</topic><topic>Natural polymers</topic><topic>Physicochemistry of polymers</topic><topic>plant extracts</topic><topic>Plant physiology and development</topic><topic>Pueraria lobata</topic><topic>Pueraria montana var. lobata</topic><topic>roots</topic><topic>Starch and polysaccharides</topic><topic>sulfate derivatives</topic><topic>Sulfated polysaccharide</topic><topic>sulfates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cui, Hengxiang</creatorcontrib><creatorcontrib>Liu, Qin</creatorcontrib><creatorcontrib>Tao, Yongzhen</creatorcontrib><creatorcontrib>Zhang, Hongfeng</creatorcontrib><creatorcontrib>Zhang, Lina</creatorcontrib><creatorcontrib>Ding, Kan</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Carbohydrate polymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cui, Hengxiang</au><au>Liu, Qin</au><au>Tao, Yongzhen</au><au>Zhang, Hongfeng</au><au>Zhang, Lina</au><au>Ding, Kan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structure and chain conformation of a (1 → 6)-α- d-glucan from the root of Pueraria lobata (Willd.) Ohwi and the antioxidant activity of its sulfated derivative</atitle><jtitle>Carbohydrate polymers</jtitle><date>2008-11-21</date><risdate>2008</risdate><volume>74</volume><issue>4</issue><spage>771</spage><epage>778</epage><pages>771-778</pages><issn>0144-8617</issn><eissn>1879-1344</eissn><coden>CAPOD8</coden><abstract>A water soluble glucan, PLB-2C, was isolated from the water extract of the root of
Pueraria lobata (Willd) Ohwi using anion-exchange and gel permeation chromatography. Its structure was investigated by gas chromatography (GC), gas chromatography–mass spectrometry (GC–MS), infrared (IR) spectra, and nuclear magnetic resonance (NMR) spectroscopy of heteronuclear single quantum coherence (HSQC) and heteronuclear multiple bond correlation (HMBC) techniques. The results indicated that PLB-2C was a linear glucan composed of (1
→
6)-α-
d-Glc
p. Chain conformation study showed that the polysaccharide took random coil compact conformation.
In vitro cell viability assay by MTT method, its sulfated derivative PLB-2CS which was substituted at 2-O, 3-O, 4-O positions, at 0.1, 1, and 5
mg/ml, could attenuate PC12 cell damage significantly caused by hydrogen peroxide.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><doi>10.1016/j.carbpol.2008.04.034</doi><tpages>8</tpages></addata></record> |
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| source | Elsevier ScienceDirect Journals |
| subjects | (1 → 6)-α- d-Glucan antioxidant activity Antioxidant effect Applied sciences Biological and medical sciences biopolymers Chain conformation chemical composition Chemical constitution chemical structure Exact sciences and technology Fundamental and applied biological sciences. Psychology glucans herbal medicines Natural polymers Physicochemistry of polymers plant extracts Plant physiology and development Pueraria lobata Pueraria montana var. lobata roots Starch and polysaccharides sulfate derivatives Sulfated polysaccharide sulfates |
| title | Structure and chain conformation of a (1 → 6)-α- d-glucan from the root of Pueraria lobata (Willd.) Ohwi and the antioxidant activity of its sulfated derivative |
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