Protocorm-like body (PLB) formation and plant regeneration from the callus culture of Dendrobium candidum Wall ex Lindl
An efficient system was established for a higher frequency of protocorm-like body (PLB) formation from the callus of Dendrobium candidum Wall ex Lindl. The calluses were induced from longitudinally bisected segments of protocorms and subcultured two times every 40d on Murashige and Skoog medium with...
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| Veröffentlicht in: | In vitro cellular & developmental biology. Plant 2008-06, Vol.44 (3), p.178-185 |
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| creator | Zhao, Peng Wu, Fei Feng, Fo-Sheng Wang, Wan-Jun |
| description | An efficient system was established for a higher frequency of protocorm-like body (PLB) formation from the callus of Dendrobium candidum Wall ex Lindl. The calluses were induced from longitudinally bisected segments of protocorms and subcultured two times every 40d on Murashige and Skoog medium with macronutrients at half strength, micronutrients at full strength, 2% sucrose, and with 8.8μM 6-Benzylaminopurine. PLB formation was achieved when calluses were transferred onto the same basal medium without any plant growth regulators. PLBs developed into intact plantlets about 2cm in height and with four roots when on basal medium with 2.7μM 1-naphthaleneacetic acid. Plantlets were transplanted into vermiculite with a 95% survival rate in a greenhouse. Histological observations proved that globular somatic embryos could be produced from the inside and surface of the embryogenic callus during PLB formation. |
| doi_str_mv | 10.1007/s11627-007-9101-2 |
| format | Article |
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The calluses were induced from longitudinally bisected segments of protocorms and subcultured two times every 40d on Murashige and Skoog medium with macronutrients at half strength, micronutrients at full strength, 2% sucrose, and with 8.8μM 6-Benzylaminopurine. PLB formation was achieved when calluses were transferred onto the same basal medium without any plant growth regulators. PLBs developed into intact plantlets about 2cm in height and with four roots when on basal medium with 2.7μM 1-naphthaleneacetic acid. Plantlets were transplanted into vermiculite with a 95% survival rate in a greenhouse. Histological observations proved that globular somatic embryos could be produced from the inside and surface of the embryogenic callus during PLB formation.</description><identifier>ISSN: 1054-5476</identifier><identifier>EISSN: 1475-2689</identifier><identifier>DOI: 10.1007/s11627-007-9101-2</identifier><language>eng</language><publisher>New York: The Society for In Vitro Biology</publisher><subject>benzyladenine ; Biomedical and Life Sciences ; Callus ; callus culture ; Cell Biology ; culture media ; Dendrobium ; Developmental Biology ; DEVELOPMENTAL BIOLOGY/MORPHOGENESIS ; Differentiation ; Ethanol ; greenhouses ; Growth regulators ; Life Sciences ; Mass propagation ; Micronutrients ; Morphogenesis ; naphthaleneacetic acid ; Orchid ; Plant Breeding/Biotechnology ; Plant cells ; Plant Genetics and Genomics ; Plant growth ; Plant growth regulators ; Plant propagation ; Plant Sciences ; Plantlets ; Plants ; Protocorms ; Regeneration ; roots ; Somatic embryogenesis ; Somatic embryos ; sucrose ; Survival ; survival rate ; vermiculite</subject><ispartof>In vitro cellular & developmental biology. Plant, 2008-06, Vol.44 (3), p.178-185</ispartof><rights>The Society for In Vitro Biology 2008</rights><rights>Copyright 2008 Society for in vitro Biology</rights><rights>The Society for In Vitro Biology 2007</rights><rights>Copyright Society for In Vitro Biology May/Jun 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b432t-9e036080e5eb69e8a3a6e40dea7b95dc4af68745ce3939c5e2a972b45c15aabf3</citedby><cites>FETCH-LOGICAL-b432t-9e036080e5eb69e8a3a6e40dea7b95dc4af68745ce3939c5e2a972b45c15aabf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://bioone.org/doi/pdf/10.1007/s11627-007-9101-2$$EPDF$$P50$$Gbioone$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/20461704$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,26978,27924,27925,41488,42557,51319,52363,58017,58250</link.rule.ids></links><search><contributor>Bunn, E.</contributor><creatorcontrib>Zhao, Peng</creatorcontrib><creatorcontrib>Wu, Fei</creatorcontrib><creatorcontrib>Feng, Fo-Sheng</creatorcontrib><creatorcontrib>Wang, Wan-Jun</creatorcontrib><title>Protocorm-like body (PLB) formation and plant regeneration from the callus culture of Dendrobium candidum Wall ex Lindl</title><title>In vitro cellular & developmental biology. Plant</title><addtitle>In Vitro Cell.Dev.Biol.-Plant</addtitle><description>An efficient system was established for a higher frequency of protocorm-like body (PLB) formation from the callus of Dendrobium candidum Wall ex Lindl. The calluses were induced from longitudinally bisected segments of protocorms and subcultured two times every 40d on Murashige and Skoog medium with macronutrients at half strength, micronutrients at full strength, 2% sucrose, and with 8.8μM 6-Benzylaminopurine. PLB formation was achieved when calluses were transferred onto the same basal medium without any plant growth regulators. PLBs developed into intact plantlets about 2cm in height and with four roots when on basal medium with 2.7μM 1-naphthaleneacetic acid. Plantlets were transplanted into vermiculite with a 95% survival rate in a greenhouse. Histological observations proved that globular somatic embryos could be produced from the inside and surface of the embryogenic callus during PLB formation.</description><subject>benzyladenine</subject><subject>Biomedical and Life Sciences</subject><subject>Callus</subject><subject>callus culture</subject><subject>Cell Biology</subject><subject>culture media</subject><subject>Dendrobium</subject><subject>Developmental Biology</subject><subject>DEVELOPMENTAL BIOLOGY/MORPHOGENESIS</subject><subject>Differentiation</subject><subject>Ethanol</subject><subject>greenhouses</subject><subject>Growth regulators</subject><subject>Life Sciences</subject><subject>Mass propagation</subject><subject>Micronutrients</subject><subject>Morphogenesis</subject><subject>naphthaleneacetic acid</subject><subject>Orchid</subject><subject>Plant Breeding/Biotechnology</subject><subject>Plant cells</subject><subject>Plant Genetics and Genomics</subject><subject>Plant growth</subject><subject>Plant growth regulators</subject><subject>Plant propagation</subject><subject>Plant Sciences</subject><subject>Plantlets</subject><subject>Plants</subject><subject>Protocorms</subject><subject>Regeneration</subject><subject>roots</subject><subject>Somatic embryogenesis</subject><subject>Somatic embryos</subject><subject>sucrose</subject><subject>Survival</subject><subject>survival rate</subject><subject>vermiculite</subject><issn>1054-5476</issn><issn>1475-2689</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkU9v1DAQxSNEJUrhA3BAWBxQOZiO_8XxEVqglVaiElQcLSeZLF4Se2sngn57vApCFQfEyU8zvzfzrKmqZwzeMAB9lhmruaZFUsOAUf6gOmZSK8rrxjwsGpSkSur6UfU45x0AFEofVz-uU5xjF9NER_8dSRv7O3J6vXn3mgyl6GYfA3GhJ_vRhZkk3GLAtJaHFCcyf0PSuXFcMumWcV4SkjiQCwx9iq1fptIMve-L-Foogj_Jxod-fFIdDW7M-PT3e1LdfHj_5fySbj59vDp_u6GtFHymBkHU0AAqbGuDjROuRgk9Ot0a1XfSDXWjpepQGGE6hdwZzdtSYMq5dhAn1at17j7F2wXzbCefOxzLbzAu2TIjgQsuC_jyL3AXlxRKtsJo0ahGNwViK9SlmHPCwe6Tn1y6swzs4Q52vYM9yMMdLC8evnpyYcMW073B_zA9X027PMf0ZwsHWTMNh7Qv1v7gonXb5LO9-cyBKQAOQhsoxNlKtD7GgP8R9BdJP6yQ</recordid><startdate>20080601</startdate><enddate>20080601</enddate><creator>Zhao, Peng</creator><creator>Wu, Fei</creator><creator>Feng, Fo-Sheng</creator><creator>Wang, Wan-Jun</creator><general>The Society for In Vitro Biology</general><general>Springer</general><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>4U-</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>S0X</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20080601</creationdate><title>Protocorm-like body (PLB) formation and plant regeneration from the callus culture of Dendrobium candidum Wall ex Lindl</title><author>Zhao, Peng ; Wu, Fei ; Feng, Fo-Sheng ; Wang, Wan-Jun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b432t-9e036080e5eb69e8a3a6e40dea7b95dc4af68745ce3939c5e2a972b45c15aabf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>benzyladenine</topic><topic>Biomedical and Life Sciences</topic><topic>Callus</topic><topic>callus culture</topic><topic>Cell Biology</topic><topic>culture media</topic><topic>Dendrobium</topic><topic>Developmental Biology</topic><topic>DEVELOPMENTAL BIOLOGY/MORPHOGENESIS</topic><topic>Differentiation</topic><topic>Ethanol</topic><topic>greenhouses</topic><topic>Growth regulators</topic><topic>Life Sciences</topic><topic>Mass propagation</topic><topic>Micronutrients</topic><topic>Morphogenesis</topic><topic>naphthaleneacetic acid</topic><topic>Orchid</topic><topic>Plant Breeding/Biotechnology</topic><topic>Plant cells</topic><topic>Plant Genetics and Genomics</topic><topic>Plant growth</topic><topic>Plant growth regulators</topic><topic>Plant propagation</topic><topic>Plant Sciences</topic><topic>Plantlets</topic><topic>Plants</topic><topic>Protocorms</topic><topic>Regeneration</topic><topic>roots</topic><topic>Somatic embryogenesis</topic><topic>Somatic embryos</topic><topic>sucrose</topic><topic>Survival</topic><topic>survival rate</topic><topic>vermiculite</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Peng</creatorcontrib><creatorcontrib>Wu, Fei</creatorcontrib><creatorcontrib>Feng, Fo-Sheng</creatorcontrib><creatorcontrib>Wang, Wan-Jun</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Docstoc</collection><collection>University Readers</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>In vitro cellular & developmental biology. Plant</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Peng</au><au>Wu, Fei</au><au>Feng, Fo-Sheng</au><au>Wang, Wan-Jun</au><au>Bunn, E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protocorm-like body (PLB) formation and plant regeneration from the callus culture of Dendrobium candidum Wall ex Lindl</atitle><jtitle>In vitro cellular & developmental biology. Plant</jtitle><stitle>In Vitro Cell.Dev.Biol.-Plant</stitle><date>2008-06-01</date><risdate>2008</risdate><volume>44</volume><issue>3</issue><spage>178</spage><epage>185</epage><pages>178-185</pages><issn>1054-5476</issn><eissn>1475-2689</eissn><abstract>An efficient system was established for a higher frequency of protocorm-like body (PLB) formation from the callus of Dendrobium candidum Wall ex Lindl. The calluses were induced from longitudinally bisected segments of protocorms and subcultured two times every 40d on Murashige and Skoog medium with macronutrients at half strength, micronutrients at full strength, 2% sucrose, and with 8.8μM 6-Benzylaminopurine. PLB formation was achieved when calluses were transferred onto the same basal medium without any plant growth regulators. PLBs developed into intact plantlets about 2cm in height and with four roots when on basal medium with 2.7μM 1-naphthaleneacetic acid. Plantlets were transplanted into vermiculite with a 95% survival rate in a greenhouse. Histological observations proved that globular somatic embryos could be produced from the inside and surface of the embryogenic callus during PLB formation.</abstract><cop>New York</cop><pub>The Society for In Vitro Biology</pub><doi>10.1007/s11627-007-9101-2</doi><tpages>8</tpages></addata></record> |
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| ispartof | In vitro cellular & developmental biology. Plant, 2008-06, Vol.44 (3), p.178-185 |
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| subjects | benzyladenine Biomedical and Life Sciences Callus callus culture Cell Biology culture media Dendrobium Developmental Biology DEVELOPMENTAL BIOLOGY/MORPHOGENESIS Differentiation Ethanol greenhouses Growth regulators Life Sciences Mass propagation Micronutrients Morphogenesis naphthaleneacetic acid Orchid Plant Breeding/Biotechnology Plant cells Plant Genetics and Genomics Plant growth Plant growth regulators Plant propagation Plant Sciences Plantlets Plants Protocorms Regeneration roots Somatic embryogenesis Somatic embryos sucrose Survival survival rate vermiculite |
| title | Protocorm-like body (PLB) formation and plant regeneration from the callus culture of Dendrobium candidum Wall ex Lindl |
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