Preparation and characterization of gelatin/α-TCP/SF biocomposite scaffold for bone tissue regeneration
In this study, we suggest a new biocomposite scaffold composed of gelatin/α-TCP (tricalcium phosphate)/SF (silk-fibroin) (GTS) which has enhanced mechanical strength and high level of cellular activity. To fabricate GTS scaffold, a temperature-controlled 3D printing process was used and appropriate...
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| Veröffentlicht in: | International journal of biological macromolecules 2018-04, Vol.110, p.488-496 |
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| container_title | International journal of biological macromolecules |
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| creator | Huh, JunTae Lee, JiUn Kim, WonJin Yeo, Miji Kim, GeunHyung |
| description | In this study, we suggest a new biocomposite scaffold composed of gelatin/α-TCP (tricalcium phosphate)/SF (silk-fibroin) (GTS) which has enhanced mechanical strength and high level of cellular activity. To fabricate GTS scaffold, a temperature-controlled 3D printing process was used and appropriate printing conditions were selected based on rheological data. To show the feasibility as a biomedical scaffold for bone tissue regeneration, the various physical and biological results, using MG63 (osteoblast-like cells), of the GTS scaffold were compared with those of a pure gelatin (G) and gelatin/α-TCP (GT) composite scaffold. GTS scaffolds showed enhanced mechanical properties in dry and wet state compared to those of the G and GT scaffolds. Also, significantly high cell-proliferation and differentiation of MG63 cells were observed in the GTS scaffold. Therefore, the GTS composite scaffold will be one of highly potential biomaterials to be used in bone regeneration. |
| doi_str_mv | 10.1016/j.ijbiomac.2017.09.030 |
| format | Article |
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Therefore, the GTS composite scaffold will be one of highly potential biomaterials to be used in bone regeneration.</description><identifier>ISSN: 0141-8130</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2017.09.030</identifier><identifier>PMID: 28917939</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Biocomposite ; Bone and Bones - cytology ; Bone and Bones - metabolism ; Bone Regeneration ; Calcium Phosphates - chemistry ; Cell Line ; Fibroins - chemistry ; Gelatin ; Gelatin - chemistry ; Humans ; Osteoblasts - cytology ; Osteoblasts - metabolism ; Scaffold ; Silk fibroin ; Tissue engineering ; Tissue Scaffolds - chemistry ; Tricalcium phosphate</subject><ispartof>International journal of biological macromolecules, 2018-04, Vol.110, p.488-496</ispartof><rights>2017 Elsevier B.V.</rights><rights>Copyright © 2017 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-d4f14fe406f5cd6a9b5418e6a2b1c6dc04bb87e1554ffb026dcd17d8af8dfbff3</citedby><cites>FETCH-LOGICAL-c368t-d4f14fe406f5cd6a9b5418e6a2b1c6dc04bb87e1554ffb026dcd17d8af8dfbff3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0141813017329926$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28917939$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huh, JunTae</creatorcontrib><creatorcontrib>Lee, JiUn</creatorcontrib><creatorcontrib>Kim, WonJin</creatorcontrib><creatorcontrib>Yeo, Miji</creatorcontrib><creatorcontrib>Kim, GeunHyung</creatorcontrib><title>Preparation and characterization of gelatin/α-TCP/SF biocomposite scaffold for bone tissue regeneration</title><title>International journal of biological macromolecules</title><addtitle>Int J Biol Macromol</addtitle><description>In this study, we suggest a new biocomposite scaffold composed of gelatin/α-TCP (tricalcium phosphate)/SF (silk-fibroin) (GTS) which has enhanced mechanical strength and high level of cellular activity. To fabricate GTS scaffold, a temperature-controlled 3D printing process was used and appropriate printing conditions were selected based on rheological data. To show the feasibility as a biomedical scaffold for bone tissue regeneration, the various physical and biological results, using MG63 (osteoblast-like cells), of the GTS scaffold were compared with those of a pure gelatin (G) and gelatin/α-TCP (GT) composite scaffold. GTS scaffolds showed enhanced mechanical properties in dry and wet state compared to those of the G and GT scaffolds. Also, significantly high cell-proliferation and differentiation of MG63 cells were observed in the GTS scaffold. Therefore, the GTS composite scaffold will be one of highly potential biomaterials to be used in bone regeneration.</description><subject>Biocomposite</subject><subject>Bone and Bones - cytology</subject><subject>Bone and Bones - metabolism</subject><subject>Bone Regeneration</subject><subject>Calcium Phosphates - chemistry</subject><subject>Cell Line</subject><subject>Fibroins - chemistry</subject><subject>Gelatin</subject><subject>Gelatin - chemistry</subject><subject>Humans</subject><subject>Osteoblasts - cytology</subject><subject>Osteoblasts - metabolism</subject><subject>Scaffold</subject><subject>Silk fibroin</subject><subject>Tissue engineering</subject><subject>Tissue Scaffolds - chemistry</subject><subject>Tricalcium phosphate</subject><issn>0141-8130</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1uFDEQhS0EIkPgCpGXbLqnavrP3oFGJEGKRCTC2vJPOfGouz3YPUhwKy7CmXDUCVtWVfX0qp7qY-wCoUbAfnuow8GEOGlb7wCHGmQNDbxgGxSDrACgeck2gC1WAhs4Y29yPhS171C8Zmc7IXGQjdywh9tER530EuLM9ey4fSiTXSiFX6sYPb-nsfTz9s_v6m5_u_16yUu0jdMx5rAQz1Z7H0fHfUzcxJn4EnI-EU90TzOtx9-yV16Pmd491XP27fLT3f66uvly9Xn_8aayTS-WyrUeW08t9L6zrtfSdC0K6vXOoO2dhdYYMRB2Xeu9gV2RHA5OaC-cN9435-z9eveY4vcT5UVNIVsaRz1TPGWFsgWUcgBRrP1qtSnmnMirYwqTTj8VgnqkrA7qmbJ6pKxAqkK5LF48ZZzMRO7f2jPWYviwGqh8-iNQUtkGmi25kMguysXwv4y_1GOU8w</recordid><startdate>20180415</startdate><enddate>20180415</enddate><creator>Huh, JunTae</creator><creator>Lee, JiUn</creator><creator>Kim, WonJin</creator><creator>Yeo, Miji</creator><creator>Kim, GeunHyung</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20180415</creationdate><title>Preparation and characterization of gelatin/α-TCP/SF biocomposite scaffold for bone tissue regeneration</title><author>Huh, JunTae ; Lee, JiUn ; Kim, WonJin ; Yeo, Miji ; Kim, GeunHyung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-d4f14fe406f5cd6a9b5418e6a2b1c6dc04bb87e1554ffb026dcd17d8af8dfbff3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Biocomposite</topic><topic>Bone and Bones - cytology</topic><topic>Bone and Bones - metabolism</topic><topic>Bone Regeneration</topic><topic>Calcium Phosphates - chemistry</topic><topic>Cell Line</topic><topic>Fibroins - chemistry</topic><topic>Gelatin</topic><topic>Gelatin - chemistry</topic><topic>Humans</topic><topic>Osteoblasts - cytology</topic><topic>Osteoblasts - metabolism</topic><topic>Scaffold</topic><topic>Silk fibroin</topic><topic>Tissue engineering</topic><topic>Tissue Scaffolds - chemistry</topic><topic>Tricalcium phosphate</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huh, JunTae</creatorcontrib><creatorcontrib>Lee, JiUn</creatorcontrib><creatorcontrib>Kim, WonJin</creatorcontrib><creatorcontrib>Yeo, Miji</creatorcontrib><creatorcontrib>Kim, GeunHyung</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of biological macromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huh, JunTae</au><au>Lee, JiUn</au><au>Kim, WonJin</au><au>Yeo, Miji</au><au>Kim, GeunHyung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation and characterization of gelatin/α-TCP/SF biocomposite scaffold for bone tissue regeneration</atitle><jtitle>International journal of biological macromolecules</jtitle><addtitle>Int J Biol Macromol</addtitle><date>2018-04-15</date><risdate>2018</risdate><volume>110</volume><spage>488</spage><epage>496</epage><pages>488-496</pages><issn>0141-8130</issn><eissn>1879-0003</eissn><abstract>In this study, we suggest a new biocomposite scaffold composed of gelatin/α-TCP (tricalcium phosphate)/SF (silk-fibroin) (GTS) which has enhanced mechanical strength and high level of cellular activity. To fabricate GTS scaffold, a temperature-controlled 3D printing process was used and appropriate printing conditions were selected based on rheological data. To show the feasibility as a biomedical scaffold for bone tissue regeneration, the various physical and biological results, using MG63 (osteoblast-like cells), of the GTS scaffold were compared with those of a pure gelatin (G) and gelatin/α-TCP (GT) composite scaffold. GTS scaffolds showed enhanced mechanical properties in dry and wet state compared to those of the G and GT scaffolds. Also, significantly high cell-proliferation and differentiation of MG63 cells were observed in the GTS scaffold. Therefore, the GTS composite scaffold will be one of highly potential biomaterials to be used in bone regeneration.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>28917939</pmid><doi>10.1016/j.ijbiomac.2017.09.030</doi><tpages>9</tpages></addata></record> |
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| ispartof | International journal of biological macromolecules, 2018-04, Vol.110, p.488-496 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Biocomposite Bone and Bones - cytology Bone and Bones - metabolism Bone Regeneration Calcium Phosphates - chemistry Cell Line Fibroins - chemistry Gelatin Gelatin - chemistry Humans Osteoblasts - cytology Osteoblasts - metabolism Scaffold Silk fibroin Tissue engineering Tissue Scaffolds - chemistry Tricalcium phosphate |
| title | Preparation and characterization of gelatin/α-TCP/SF biocomposite scaffold for bone tissue regeneration |
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