Magnesium (Mg) based interference screws developed for promoting tendon graft incorporation in bone tunnel in rabbits
[Display omitted] How to enhance tendon graft incorporation into bone tunnels for achieving satisfactory healing outcomes in patients with anterior cruciate ligament reconstruction (ACLR) is one of the most challenging clinical problems in orthopaedic sports medicine. Several studies have recently r...
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| creator | Wang, Jiali Xu, Jiankun Song, Bin Chow, Dick Hokiu Shu-hang Yung, Patrick Qin, Ling |
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How to enhance tendon graft incorporation into bone tunnels for achieving satisfactory healing outcomes in patients with anterior cruciate ligament reconstruction (ACLR) is one of the most challenging clinical problems in orthopaedic sports medicine. Several studies have recently reported the beneficial effects of Mg implants in bone fracture healing, indicating the use potential of Mg devices in promoting the tendon graft osteointegration. Here, we developed an innovative Mg-based interference screws for fixation of the tendon graft in rabbits underwent ACLR and investigated the biological role of Mg-based implants in the graft healing. The titanium (Ti) interference screw was used as the control. We demonstrated that Mg interference screw significantly accelerated the incorporation of the tendon graft into bone tunnels via multiscale analytical methods including scanning electronic microscopy/energy dispersive spectrometer (SEM/EDS), micro-hardness, micro-Fourier transform infrared spectroscopy (μFTIR), and histology. Our in vivo study showed that Mg implants enhanced the recruitment of bone marrow stromal stem cells (BMSCs) towards peri-implant bone tissue, which may be ascribed to the upregulation of local TGF-β1 and PDGF-BB. Besides, the in vitro study revealed that higher Mg ions was beneficial to the improvement of capability in cell adhesion and osteogenic differentiation of BMSCs. Thus, the enhancement in cell migration, cell adhesion and osteogenic differentiation of BMSCs may contribute to an improved tendon graft osteointegration in the Mg group. Our findings in this work may further facilitate clinical applications of Mg-based interference screws for enhancing tendon graft-bone junction healing in patients indicated for ACLR.
How to promote tendon-bone junction healing is one of the major challenging issues for satisfactory clinical outcomes in patients after ACL reconstruction. The improvement of bony ingrowth into the tendon graft-bone interface can enhance the tendon graft osteointegration. In this study, we applied Mg based interference screws to fix the tendon graft in rabbits and found the use of Mg screws could accelerate and significantly increase mineralized matrix formation at the tendon-bone interface in animals when compared to those with Ti screws. We elucidated the mechanism behind the favorable effects of Mg screws on the graft healing in both in vitro and in vivo studies from multiscale technologies. The optim |
| doi_str_mv | 10.1016/j.actbio.2017.09.018 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1940195612</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1742706117305822</els_id><sourcerecordid>1940195612</sourcerecordid><originalsourceid>FETCH-LOGICAL-c427t-fdc1588e4ce5b12c6520fea92a70572ea8f6d04c8eaf0f80b19d28f103fc5d903</originalsourceid><addsrcrecordid>eNp9kU-PFCEQxYnRuOvqNzCGxMt66LZguhu4mJiN_5LdeNEzoaGYMOmGEeg1fnuZzOrBgycK6veKynuEvGTQM2DT20NvbJ1D6jkw0YPqgclH5JJJITsxTvJxq8XAOwETuyDPSjkA7CTj8im54FIxNTK4JNud2UcsYVvp9d3-DZ1NQUdDrJg9ZowWabEZfxbq8B6XdGxdnzI95rSmGuKeVowuRbrPxtcmtCkfUzY1tLcQ6Zwi0rrFiMvpms08h1qekyfeLAVfPJxX5PvHD99uPne3Xz99uXl_29mBi9p5Z9koJQ4Wx5lxO40cPBrFjYBRcDTSTw4GK9F48BJmphyXnsHO29Ep2F2R6_Pctu6PDUvVaygWl8VETFvRTA3QjJgYb-jrf9BD2nJs2zVKgBomyWWjhjNlcyolo9fHHFaTf2kG-hSLPuhzLPoUiwalWyxN9uph-Dav6P6K_uTQgHdnAJsb9wGzLjac3Hcho63apfD_H34D7dmhlQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1970946828</pqid></control><display><type>article</type><title>Magnesium (Mg) based interference screws developed for promoting tendon graft incorporation in bone tunnel in rabbits</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Wang, Jiali ; Xu, Jiankun ; Song, Bin ; Chow, Dick Hokiu ; Shu-hang Yung, Patrick ; Qin, Ling</creator><creatorcontrib>Wang, Jiali ; Xu, Jiankun ; Song, Bin ; Chow, Dick Hokiu ; Shu-hang Yung, Patrick ; Qin, Ling</creatorcontrib><description>[Display omitted]
How to enhance tendon graft incorporation into bone tunnels for achieving satisfactory healing outcomes in patients with anterior cruciate ligament reconstruction (ACLR) is one of the most challenging clinical problems in orthopaedic sports medicine. Several studies have recently reported the beneficial effects of Mg implants in bone fracture healing, indicating the use potential of Mg devices in promoting the tendon graft osteointegration. Here, we developed an innovative Mg-based interference screws for fixation of the tendon graft in rabbits underwent ACLR and investigated the biological role of Mg-based implants in the graft healing. The titanium (Ti) interference screw was used as the control. We demonstrated that Mg interference screw significantly accelerated the incorporation of the tendon graft into bone tunnels via multiscale analytical methods including scanning electronic microscopy/energy dispersive spectrometer (SEM/EDS), micro-hardness, micro-Fourier transform infrared spectroscopy (μFTIR), and histology. Our in vivo study showed that Mg implants enhanced the recruitment of bone marrow stromal stem cells (BMSCs) towards peri-implant bone tissue, which may be ascribed to the upregulation of local TGF-β1 and PDGF-BB. Besides, the in vitro study revealed that higher Mg ions was beneficial to the improvement of capability in cell adhesion and osteogenic differentiation of BMSCs. Thus, the enhancement in cell migration, cell adhesion and osteogenic differentiation of BMSCs may contribute to an improved tendon graft osteointegration in the Mg group. Our findings in this work may further facilitate clinical applications of Mg-based interference screws for enhancing tendon graft-bone junction healing in patients indicated for ACLR.
How to promote tendon-bone junction healing is one of the major challenging issues for satisfactory clinical outcomes in patients after ACL reconstruction. The improvement of bony ingrowth into the tendon graft-bone interface can enhance the tendon graft osteointegration. In this study, we applied Mg based interference screws to fix the tendon graft in rabbits and found the use of Mg screws could accelerate and significantly increase mineralized matrix formation at the tendon-bone interface in animals when compared to those with Ti screws. We elucidated the mechanism behind the favorable effects of Mg screws on the graft healing in both in vitro and in vivo studies from multiscale technologies. The optimized interface structure and function in Mg group may be ascribed to the improved cell migration capability, enhanced cell adhesion strength and promoted osteogenic differentiation ability of BMSCs under the stimuli of Mg ions degraded from implanted Mg screws. Our findings may help us broaden our thinking in the application potential of Mg interference screws in future clinical trials.</description><identifier>ISSN: 1742-7061</identifier><identifier>EISSN: 1878-7568</identifier><identifier>DOI: 10.1016/j.actbio.2017.09.018</identifier><identifier>PMID: 28919510</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>ACL ; Adhesion ; Analytical methods ; Animals ; Anterior cruciate ligament ; Anterior Cruciate Ligament - drug effects ; Anterior Cruciate Ligament - surgery ; Anterior Cruciate Ligament Reconstruction ; Biocompatibility ; Biodegradable ; Biomedical materials ; Bone and Bones - drug effects ; Bone and Bones - physiology ; Bone healing ; Bone implants ; Bone marrow ; Bone Screws ; Bones ; Cell adhesion ; Cell Adhesion - drug effects ; Cell Differentiation - drug effects ; Cell migration ; Cells, Cultured ; Corrosion ; Differentiation ; Fourier transforms ; Grafting ; Hardness ; Healing ; Histology ; Implants, Experimental ; In vivo methods and tests ; Infrared spectroscopy ; Interference ; Knee Joint - diagnostic imaging ; Knee Joint - pathology ; Magnesium ; Magnesium - pharmacology ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - drug effects ; Microhardness ; Microscopy ; Minerals - metabolism ; Multiscale analysis ; Osseointegration ; Osseointegration - drug effects ; Osteoclasts - drug effects ; Osteoclasts - metabolism ; Osteogenesis - drug effects ; Patients ; Platelet-derived growth factor ; Platelet-derived growth factor BB ; Rabbits ; Rats ; Recruitment ; Scanning transmission electron microscopy ; Screws ; Spectroscopy, Fourier Transform Infrared ; Sports medicine ; Stem cells ; Structure-function ; Studies ; Surgical implants ; Tartrate-Resistant Acid Phosphatase - metabolism ; Tendon graft-bone junction ; Tendons - drug effects ; Tendons - transplantation ; Therapeutic applications ; Titanium ; Tomography, X-Ray Computed ; Transforming growth factor-b1 ; Transplants & implants ; Tunnels ; Wound Healing - drug effects</subject><ispartof>Acta biomaterialia, 2017-11, Vol.63, p.393-410</ispartof><rights>2017 Acta Materialia Inc.</rights><rights>Copyright © 2017 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier BV Nov 2017</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c427t-fdc1588e4ce5b12c6520fea92a70572ea8f6d04c8eaf0f80b19d28f103fc5d903</citedby><cites>FETCH-LOGICAL-c427t-fdc1588e4ce5b12c6520fea92a70572ea8f6d04c8eaf0f80b19d28f103fc5d903</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.actbio.2017.09.018$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28919510$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Jiali</creatorcontrib><creatorcontrib>Xu, Jiankun</creatorcontrib><creatorcontrib>Song, Bin</creatorcontrib><creatorcontrib>Chow, Dick Hokiu</creatorcontrib><creatorcontrib>Shu-hang Yung, Patrick</creatorcontrib><creatorcontrib>Qin, Ling</creatorcontrib><title>Magnesium (Mg) based interference screws developed for promoting tendon graft incorporation in bone tunnel in rabbits</title><title>Acta biomaterialia</title><addtitle>Acta Biomater</addtitle><description>[Display omitted]
How to enhance tendon graft incorporation into bone tunnels for achieving satisfactory healing outcomes in patients with anterior cruciate ligament reconstruction (ACLR) is one of the most challenging clinical problems in orthopaedic sports medicine. Several studies have recently reported the beneficial effects of Mg implants in bone fracture healing, indicating the use potential of Mg devices in promoting the tendon graft osteointegration. Here, we developed an innovative Mg-based interference screws for fixation of the tendon graft in rabbits underwent ACLR and investigated the biological role of Mg-based implants in the graft healing. The titanium (Ti) interference screw was used as the control. We demonstrated that Mg interference screw significantly accelerated the incorporation of the tendon graft into bone tunnels via multiscale analytical methods including scanning electronic microscopy/energy dispersive spectrometer (SEM/EDS), micro-hardness, micro-Fourier transform infrared spectroscopy (μFTIR), and histology. Our in vivo study showed that Mg implants enhanced the recruitment of bone marrow stromal stem cells (BMSCs) towards peri-implant bone tissue, which may be ascribed to the upregulation of local TGF-β1 and PDGF-BB. Besides, the in vitro study revealed that higher Mg ions was beneficial to the improvement of capability in cell adhesion and osteogenic differentiation of BMSCs. Thus, the enhancement in cell migration, cell adhesion and osteogenic differentiation of BMSCs may contribute to an improved tendon graft osteointegration in the Mg group. Our findings in this work may further facilitate clinical applications of Mg-based interference screws for enhancing tendon graft-bone junction healing in patients indicated for ACLR.
How to promote tendon-bone junction healing is one of the major challenging issues for satisfactory clinical outcomes in patients after ACL reconstruction. The improvement of bony ingrowth into the tendon graft-bone interface can enhance the tendon graft osteointegration. In this study, we applied Mg based interference screws to fix the tendon graft in rabbits and found the use of Mg screws could accelerate and significantly increase mineralized matrix formation at the tendon-bone interface in animals when compared to those with Ti screws. We elucidated the mechanism behind the favorable effects of Mg screws on the graft healing in both in vitro and in vivo studies from multiscale technologies. The optimized interface structure and function in Mg group may be ascribed to the improved cell migration capability, enhanced cell adhesion strength and promoted osteogenic differentiation ability of BMSCs under the stimuli of Mg ions degraded from implanted Mg screws. Our findings may help us broaden our thinking in the application potential of Mg interference screws in future clinical trials.</description><subject>ACL</subject><subject>Adhesion</subject><subject>Analytical methods</subject><subject>Animals</subject><subject>Anterior cruciate ligament</subject><subject>Anterior Cruciate Ligament - drug effects</subject><subject>Anterior Cruciate Ligament - surgery</subject><subject>Anterior Cruciate Ligament Reconstruction</subject><subject>Biocompatibility</subject><subject>Biodegradable</subject><subject>Biomedical materials</subject><subject>Bone and Bones - drug effects</subject><subject>Bone and Bones - physiology</subject><subject>Bone healing</subject><subject>Bone implants</subject><subject>Bone marrow</subject><subject>Bone Screws</subject><subject>Bones</subject><subject>Cell adhesion</subject><subject>Cell Adhesion - drug effects</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell migration</subject><subject>Cells, Cultured</subject><subject>Corrosion</subject><subject>Differentiation</subject><subject>Fourier transforms</subject><subject>Grafting</subject><subject>Hardness</subject><subject>Healing</subject><subject>Histology</subject><subject>Implants, Experimental</subject><subject>In vivo methods and tests</subject><subject>Infrared spectroscopy</subject><subject>Interference</subject><subject>Knee Joint - diagnostic imaging</subject><subject>Knee Joint - pathology</subject><subject>Magnesium</subject><subject>Magnesium - pharmacology</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - drug effects</subject><subject>Microhardness</subject><subject>Microscopy</subject><subject>Minerals - metabolism</subject><subject>Multiscale analysis</subject><subject>Osseointegration</subject><subject>Osseointegration - drug effects</subject><subject>Osteoclasts - drug effects</subject><subject>Osteoclasts - metabolism</subject><subject>Osteogenesis - drug effects</subject><subject>Patients</subject><subject>Platelet-derived growth factor</subject><subject>Platelet-derived growth factor BB</subject><subject>Rabbits</subject><subject>Rats</subject><subject>Recruitment</subject><subject>Scanning transmission electron microscopy</subject><subject>Screws</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>Sports medicine</subject><subject>Stem cells</subject><subject>Structure-function</subject><subject>Studies</subject><subject>Surgical implants</subject><subject>Tartrate-Resistant Acid Phosphatase - metabolism</subject><subject>Tendon graft-bone junction</subject><subject>Tendons - drug effects</subject><subject>Tendons - transplantation</subject><subject>Therapeutic applications</subject><subject>Titanium</subject><subject>Tomography, X-Ray Computed</subject><subject>Transforming growth factor-b1</subject><subject>Transplants & implants</subject><subject>Tunnels</subject><subject>Wound Healing - drug effects</subject><issn>1742-7061</issn><issn>1878-7568</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU-PFCEQxYnRuOvqNzCGxMt66LZguhu4mJiN_5LdeNEzoaGYMOmGEeg1fnuZzOrBgycK6veKynuEvGTQM2DT20NvbJ1D6jkw0YPqgclH5JJJITsxTvJxq8XAOwETuyDPSjkA7CTj8im54FIxNTK4JNud2UcsYVvp9d3-DZ1NQUdDrJg9ZowWabEZfxbq8B6XdGxdnzI95rSmGuKeVowuRbrPxtcmtCkfUzY1tLcQ6Zwi0rrFiMvpms08h1qekyfeLAVfPJxX5PvHD99uPne3Xz99uXl_29mBi9p5Z9koJQ4Wx5lxO40cPBrFjYBRcDTSTw4GK9F48BJmphyXnsHO29Ep2F2R6_Pctu6PDUvVaygWl8VETFvRTA3QjJgYb-jrf9BD2nJs2zVKgBomyWWjhjNlcyolo9fHHFaTf2kG-hSLPuhzLPoUiwalWyxN9uph-Dav6P6K_uTQgHdnAJsb9wGzLjac3Hcho63apfD_H34D7dmhlQ</recordid><startdate>20171101</startdate><enddate>20171101</enddate><creator>Wang, Jiali</creator><creator>Xu, Jiankun</creator><creator>Song, Bin</creator><creator>Chow, Dick Hokiu</creator><creator>Shu-hang Yung, Patrick</creator><creator>Qin, Ling</creator><general>Elsevier Ltd</general><general>Elsevier BV</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20171101</creationdate><title>Magnesium (Mg) based interference screws developed for promoting tendon graft incorporation in bone tunnel in rabbits</title><author>Wang, Jiali ; Xu, Jiankun ; Song, Bin ; Chow, Dick Hokiu ; Shu-hang Yung, Patrick ; Qin, Ling</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c427t-fdc1588e4ce5b12c6520fea92a70572ea8f6d04c8eaf0f80b19d28f103fc5d903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>ACL</topic><topic>Adhesion</topic><topic>Analytical methods</topic><topic>Animals</topic><topic>Anterior cruciate ligament</topic><topic>Anterior Cruciate Ligament - drug effects</topic><topic>Anterior Cruciate Ligament - surgery</topic><topic>Anterior Cruciate Ligament Reconstruction</topic><topic>Biocompatibility</topic><topic>Biodegradable</topic><topic>Biomedical materials</topic><topic>Bone and Bones - drug effects</topic><topic>Bone and Bones - physiology</topic><topic>Bone healing</topic><topic>Bone implants</topic><topic>Bone marrow</topic><topic>Bone Screws</topic><topic>Bones</topic><topic>Cell adhesion</topic><topic>Cell Adhesion - drug effects</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell migration</topic><topic>Cells, Cultured</topic><topic>Corrosion</topic><topic>Differentiation</topic><topic>Fourier transforms</topic><topic>Grafting</topic><topic>Hardness</topic><topic>Healing</topic><topic>Histology</topic><topic>Implants, Experimental</topic><topic>In vivo methods and tests</topic><topic>Infrared spectroscopy</topic><topic>Interference</topic><topic>Knee Joint - diagnostic imaging</topic><topic>Knee Joint - pathology</topic><topic>Magnesium</topic><topic>Magnesium - pharmacology</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Mesenchymal Stromal Cells - drug effects</topic><topic>Microhardness</topic><topic>Microscopy</topic><topic>Minerals - metabolism</topic><topic>Multiscale analysis</topic><topic>Osseointegration</topic><topic>Osseointegration - drug effects</topic><topic>Osteoclasts - drug effects</topic><topic>Osteoclasts - metabolism</topic><topic>Osteogenesis - drug effects</topic><topic>Patients</topic><topic>Platelet-derived growth factor</topic><topic>Platelet-derived growth factor BB</topic><topic>Rabbits</topic><topic>Rats</topic><topic>Recruitment</topic><topic>Scanning transmission electron microscopy</topic><topic>Screws</topic><topic>Spectroscopy, Fourier Transform Infrared</topic><topic>Sports medicine</topic><topic>Stem cells</topic><topic>Structure-function</topic><topic>Studies</topic><topic>Surgical implants</topic><topic>Tartrate-Resistant Acid Phosphatase - metabolism</topic><topic>Tendon graft-bone junction</topic><topic>Tendons - drug effects</topic><topic>Tendons - transplantation</topic><topic>Therapeutic applications</topic><topic>Titanium</topic><topic>Tomography, X-Ray Computed</topic><topic>Transforming growth factor-b1</topic><topic>Transplants & implants</topic><topic>Tunnels</topic><topic>Wound Healing - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Jiali</creatorcontrib><creatorcontrib>Xu, Jiankun</creatorcontrib><creatorcontrib>Song, Bin</creatorcontrib><creatorcontrib>Chow, Dick Hokiu</creatorcontrib><creatorcontrib>Shu-hang Yung, Patrick</creatorcontrib><creatorcontrib>Qin, Ling</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Acta biomaterialia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Jiali</au><au>Xu, Jiankun</au><au>Song, Bin</au><au>Chow, Dick Hokiu</au><au>Shu-hang Yung, Patrick</au><au>Qin, Ling</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Magnesium (Mg) based interference screws developed for promoting tendon graft incorporation in bone tunnel in rabbits</atitle><jtitle>Acta biomaterialia</jtitle><addtitle>Acta Biomater</addtitle><date>2017-11-01</date><risdate>2017</risdate><volume>63</volume><spage>393</spage><epage>410</epage><pages>393-410</pages><issn>1742-7061</issn><eissn>1878-7568</eissn><abstract>[Display omitted]
How to enhance tendon graft incorporation into bone tunnels for achieving satisfactory healing outcomes in patients with anterior cruciate ligament reconstruction (ACLR) is one of the most challenging clinical problems in orthopaedic sports medicine. Several studies have recently reported the beneficial effects of Mg implants in bone fracture healing, indicating the use potential of Mg devices in promoting the tendon graft osteointegration. Here, we developed an innovative Mg-based interference screws for fixation of the tendon graft in rabbits underwent ACLR and investigated the biological role of Mg-based implants in the graft healing. The titanium (Ti) interference screw was used as the control. We demonstrated that Mg interference screw significantly accelerated the incorporation of the tendon graft into bone tunnels via multiscale analytical methods including scanning electronic microscopy/energy dispersive spectrometer (SEM/EDS), micro-hardness, micro-Fourier transform infrared spectroscopy (μFTIR), and histology. Our in vivo study showed that Mg implants enhanced the recruitment of bone marrow stromal stem cells (BMSCs) towards peri-implant bone tissue, which may be ascribed to the upregulation of local TGF-β1 and PDGF-BB. Besides, the in vitro study revealed that higher Mg ions was beneficial to the improvement of capability in cell adhesion and osteogenic differentiation of BMSCs. Thus, the enhancement in cell migration, cell adhesion and osteogenic differentiation of BMSCs may contribute to an improved tendon graft osteointegration in the Mg group. Our findings in this work may further facilitate clinical applications of Mg-based interference screws for enhancing tendon graft-bone junction healing in patients indicated for ACLR.
How to promote tendon-bone junction healing is one of the major challenging issues for satisfactory clinical outcomes in patients after ACL reconstruction. The improvement of bony ingrowth into the tendon graft-bone interface can enhance the tendon graft osteointegration. In this study, we applied Mg based interference screws to fix the tendon graft in rabbits and found the use of Mg screws could accelerate and significantly increase mineralized matrix formation at the tendon-bone interface in animals when compared to those with Ti screws. We elucidated the mechanism behind the favorable effects of Mg screws on the graft healing in both in vitro and in vivo studies from multiscale technologies. The optimized interface structure and function in Mg group may be ascribed to the improved cell migration capability, enhanced cell adhesion strength and promoted osteogenic differentiation ability of BMSCs under the stimuli of Mg ions degraded from implanted Mg screws. Our findings may help us broaden our thinking in the application potential of Mg interference screws in future clinical trials.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>28919510</pmid><doi>10.1016/j.actbio.2017.09.018</doi><tpages>18</tpages></addata></record> |
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| ispartof | Acta biomaterialia, 2017-11, Vol.63, p.393-410 |
| issn | 1742-7061 1878-7568 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1940195612 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | ACL Adhesion Analytical methods Animals Anterior cruciate ligament Anterior Cruciate Ligament - drug effects Anterior Cruciate Ligament - surgery Anterior Cruciate Ligament Reconstruction Biocompatibility Biodegradable Biomedical materials Bone and Bones - drug effects Bone and Bones - physiology Bone healing Bone implants Bone marrow Bone Screws Bones Cell adhesion Cell Adhesion - drug effects Cell Differentiation - drug effects Cell migration Cells, Cultured Corrosion Differentiation Fourier transforms Grafting Hardness Healing Histology Implants, Experimental In vivo methods and tests Infrared spectroscopy Interference Knee Joint - diagnostic imaging Knee Joint - pathology Magnesium Magnesium - pharmacology Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - drug effects Microhardness Microscopy Minerals - metabolism Multiscale analysis Osseointegration Osseointegration - drug effects Osteoclasts - drug effects Osteoclasts - metabolism Osteogenesis - drug effects Patients Platelet-derived growth factor Platelet-derived growth factor BB Rabbits Rats Recruitment Scanning transmission electron microscopy Screws Spectroscopy, Fourier Transform Infrared Sports medicine Stem cells Structure-function Studies Surgical implants Tartrate-Resistant Acid Phosphatase - metabolism Tendon graft-bone junction Tendons - drug effects Tendons - transplantation Therapeutic applications Titanium Tomography, X-Ray Computed Transforming growth factor-b1 Transplants & implants Tunnels Wound Healing - drug effects |
| title | Magnesium (Mg) based interference screws developed for promoting tendon graft incorporation in bone tunnel in rabbits |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T04%3A34%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Magnesium%20(Mg)%20based%20interference%20screws%20developed%20for%20promoting%20tendon%20graft%20incorporation%20in%20bone%20tunnel%20in%20rabbits&rft.jtitle=Acta%20biomaterialia&rft.au=Wang,%20Jiali&rft.date=2017-11-01&rft.volume=63&rft.spage=393&rft.epage=410&rft.pages=393-410&rft.issn=1742-7061&rft.eissn=1878-7568&rft_id=info:doi/10.1016/j.actbio.2017.09.018&rft_dat=%3Cproquest_cross%3E1940195612%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1970946828&rft_id=info:pmid/28919510&rft_els_id=S1742706117305822&rfr_iscdi=true |