Urinary WT1‐positive cells as a non‐invasive biomarker of crescent formation
Objective The purpose of this study was to assess the relationship between urinary WT1‐positive cells (podocytes and active parietal epithelial cells) and WT1‐positive cells in renal biopsy to investigate whether urinary WT1‐positive cells are useful for detection of crescent formation. Methods Fift...
Gespeichert in:
| Veröffentlicht in: | Cytopathology (Oxford) 2017-12, Vol.28 (6), p.524-530 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 530 |
|---|---|
| container_issue | 6 |
| container_start_page | 524 |
| container_title | Cytopathology (Oxford) |
| container_volume | 28 |
| creator | Fujita, T. Sofue, T. Moritoki, M. Nishijima, Y. Tokuhara, Y. Wakisaka, H. Kushida, Y. Haba, R. Ohsaki, H. |
| description | Objective
The purpose of this study was to assess the relationship between urinary WT1‐positive cells (podocytes and active parietal epithelial cells) and WT1‐positive cells in renal biopsy to investigate whether urinary WT1‐positive cells are useful for detection of crescent formation.
Methods
Fifty‐two patients with kidney disease were investigated (15 cases with crescentic lesions and 37 cases with non‐crescentic lesions) for immunoenzyme staining using anti‐WT1 antibody for urine cytology and renal biopsy. Numbers of WT1‐positive cells in urine and renal biopsy were counted.
Results
There was no correlation between urinary WT1‐positive cells and WT1‐positive cells in renal biopsy. However, the number of urinary WT1‐positive cells in patients with crescentic lesions was significantly higher than in patients with non‐crescentic lesions. In addition, the best cut‐off value to detect patients with crescentic lesions using urinary was 5 cells/10‐mL (area under the concentration‐time curve=0.735).
Conclusions
The results of our study suggest urinary WT1‐positive cells can be used to detect patients with crescent formation using 5 cells/10‐mL cutoff value. WT1‐positive glomerular podocytes and parietal epithelial cells may be shed into urine in active glomerular disease. This study, investigating the relationship between WT1‐positive cells in urine and in the renal biopsy found no correlation; however, the results do suggest that, using a cutoff value of 5 cells/10 mL, WT1 positive urinary cells can be used to detect patients with crescent formation.
WT1 positive glomerular podocytes and parietal epithelial cells may be shed into urine in active glomerular disease. This study, investigating the relationship between WT1 positive cells in urine and in the renal biopsy found no correlation; however, the results do suggest that, using a cutoff value of 5 cells/10 mL, WT1 positive urinary cells can be used to detect patients with crescentic formation. |
| doi_str_mv | 10.1111/cyt.12460 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_1940059027</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1966847190</sourcerecordid><originalsourceid>FETCH-LOGICAL-j4240-fd879a7178d633efcfe813cfb2ba43cdcbed2d13d8df91b34944bd0d793afbd93</originalsourceid><addsrcrecordid>eNpdkUtLAzEQx4MotlYPfgFZ8OJla177yFGKLyjooUU8LckmgdTdpCbbyt78CH5GP4npQw8OAzPw_zHMzB-AcwTHKMZ13XdjhGkOD8AQkTxLMYHkEAwhy_I0y2AxACchLCBEmGFyDAa4ZIiUNBuC57k3lvs-eZmh78-vpQumM2uV1KppQsJjJtbZqBi75mGjCONa7t-UT5xOaq9CrWyXaOdb3hlnT8GR5k1QZ_s6AvO729nkIZ0-3T9ObqbpgmIKUy3LgvECFaXMCVG61qpEpNYCC05JLWuhJJaIyFJqhgShjFIhoSwY4VpIRkbgajd36d37SoWuak3YbM2tcqtQIUYhzBjERUQv_6ELt_I2bhepPC9pgRiM1MWeWolWyWrpTbyzr35_FYHrHfBhGtX_6QhWGxOqaEK1NaGavM62DfkBHjp7kA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1966847190</pqid></control><display><type>article</type><title>Urinary WT1‐positive cells as a non‐invasive biomarker of crescent formation</title><source>MEDLINE</source><source>Wiley Journals</source><creator>Fujita, T. ; Sofue, T. ; Moritoki, M. ; Nishijima, Y. ; Tokuhara, Y. ; Wakisaka, H. ; Kushida, Y. ; Haba, R. ; Ohsaki, H.</creator><creatorcontrib>Fujita, T. ; Sofue, T. ; Moritoki, M. ; Nishijima, Y. ; Tokuhara, Y. ; Wakisaka, H. ; Kushida, Y. ; Haba, R. ; Ohsaki, H.</creatorcontrib><description>Objective
The purpose of this study was to assess the relationship between urinary WT1‐positive cells (podocytes and active parietal epithelial cells) and WT1‐positive cells in renal biopsy to investigate whether urinary WT1‐positive cells are useful for detection of crescent formation.
Methods
Fifty‐two patients with kidney disease were investigated (15 cases with crescentic lesions and 37 cases with non‐crescentic lesions) for immunoenzyme staining using anti‐WT1 antibody for urine cytology and renal biopsy. Numbers of WT1‐positive cells in urine and renal biopsy were counted.
Results
There was no correlation between urinary WT1‐positive cells and WT1‐positive cells in renal biopsy. However, the number of urinary WT1‐positive cells in patients with crescentic lesions was significantly higher than in patients with non‐crescentic lesions. In addition, the best cut‐off value to detect patients with crescentic lesions using urinary was 5 cells/10‐mL (area under the concentration‐time curve=0.735).
Conclusions
The results of our study suggest urinary WT1‐positive cells can be used to detect patients with crescent formation using 5 cells/10‐mL cutoff value. WT1‐positive glomerular podocytes and parietal epithelial cells may be shed into urine in active glomerular disease. This study, investigating the relationship between WT1‐positive cells in urine and in the renal biopsy found no correlation; however, the results do suggest that, using a cutoff value of 5 cells/10 mL, WT1 positive urinary cells can be used to detect patients with crescent formation.
WT1 positive glomerular podocytes and parietal epithelial cells may be shed into urine in active glomerular disease. This study, investigating the relationship between WT1 positive cells in urine and in the renal biopsy found no correlation; however, the results do suggest that, using a cutoff value of 5 cells/10 mL, WT1 positive urinary cells can be used to detect patients with crescentic formation.</description><identifier>ISSN: 0956-5507</identifier><identifier>EISSN: 1365-2303</identifier><identifier>DOI: 10.1111/cyt.12460</identifier><identifier>PMID: 28913845</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Adult ; Biomarkers - urine ; Biopsy ; crescent formation ; Cytology ; Epithelial cells ; Female ; Humans ; immunocytochemistry ; Keratins - urine ; Kidney Diseases - diagnosis ; Kidney Diseases - urine ; Kidney transplantation ; liquid‐based cytology ; Male ; Middle Aged ; Podocytes - pathology ; Studies ; Urine ; urine cytology ; WT1 ; WT1 Proteins - metabolism</subject><ispartof>Cytopathology (Oxford), 2017-12, Vol.28 (6), p.524-530</ispartof><rights>2017 John Wiley & Sons Ltd</rights><rights>2017 John Wiley & Sons Ltd.</rights><rights>Copyright © 2017 John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0001-6046-3785</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fcyt.12460$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fcyt.12460$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28913845$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fujita, T.</creatorcontrib><creatorcontrib>Sofue, T.</creatorcontrib><creatorcontrib>Moritoki, M.</creatorcontrib><creatorcontrib>Nishijima, Y.</creatorcontrib><creatorcontrib>Tokuhara, Y.</creatorcontrib><creatorcontrib>Wakisaka, H.</creatorcontrib><creatorcontrib>Kushida, Y.</creatorcontrib><creatorcontrib>Haba, R.</creatorcontrib><creatorcontrib>Ohsaki, H.</creatorcontrib><title>Urinary WT1‐positive cells as a non‐invasive biomarker of crescent formation</title><title>Cytopathology (Oxford)</title><addtitle>Cytopathology</addtitle><description>Objective
The purpose of this study was to assess the relationship between urinary WT1‐positive cells (podocytes and active parietal epithelial cells) and WT1‐positive cells in renal biopsy to investigate whether urinary WT1‐positive cells are useful for detection of crescent formation.
Methods
Fifty‐two patients with kidney disease were investigated (15 cases with crescentic lesions and 37 cases with non‐crescentic lesions) for immunoenzyme staining using anti‐WT1 antibody for urine cytology and renal biopsy. Numbers of WT1‐positive cells in urine and renal biopsy were counted.
Results
There was no correlation between urinary WT1‐positive cells and WT1‐positive cells in renal biopsy. However, the number of urinary WT1‐positive cells in patients with crescentic lesions was significantly higher than in patients with non‐crescentic lesions. In addition, the best cut‐off value to detect patients with crescentic lesions using urinary was 5 cells/10‐mL (area under the concentration‐time curve=0.735).
Conclusions
The results of our study suggest urinary WT1‐positive cells can be used to detect patients with crescent formation using 5 cells/10‐mL cutoff value. WT1‐positive glomerular podocytes and parietal epithelial cells may be shed into urine in active glomerular disease. This study, investigating the relationship between WT1‐positive cells in urine and in the renal biopsy found no correlation; however, the results do suggest that, using a cutoff value of 5 cells/10 mL, WT1 positive urinary cells can be used to detect patients with crescent formation.
WT1 positive glomerular podocytes and parietal epithelial cells may be shed into urine in active glomerular disease. This study, investigating the relationship between WT1 positive cells in urine and in the renal biopsy found no correlation; however, the results do suggest that, using a cutoff value of 5 cells/10 mL, WT1 positive urinary cells can be used to detect patients with crescentic formation.</description><subject>Adult</subject><subject>Biomarkers - urine</subject><subject>Biopsy</subject><subject>crescent formation</subject><subject>Cytology</subject><subject>Epithelial cells</subject><subject>Female</subject><subject>Humans</subject><subject>immunocytochemistry</subject><subject>Keratins - urine</subject><subject>Kidney Diseases - diagnosis</subject><subject>Kidney Diseases - urine</subject><subject>Kidney transplantation</subject><subject>liquid‐based cytology</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Podocytes - pathology</subject><subject>Studies</subject><subject>Urine</subject><subject>urine cytology</subject><subject>WT1</subject><subject>WT1 Proteins - metabolism</subject><issn>0956-5507</issn><issn>1365-2303</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkUtLAzEQx4MotlYPfgFZ8OJla177yFGKLyjooUU8LckmgdTdpCbbyt78CH5GP4npQw8OAzPw_zHMzB-AcwTHKMZ13XdjhGkOD8AQkTxLMYHkEAwhy_I0y2AxACchLCBEmGFyDAa4ZIiUNBuC57k3lvs-eZmh78-vpQumM2uV1KppQsJjJtbZqBi75mGjCONa7t-UT5xOaq9CrWyXaOdb3hlnT8GR5k1QZ_s6AvO729nkIZ0-3T9ObqbpgmIKUy3LgvECFaXMCVG61qpEpNYCC05JLWuhJJaIyFJqhgShjFIhoSwY4VpIRkbgajd36d37SoWuak3YbM2tcqtQIUYhzBjERUQv_6ELt_I2bhepPC9pgRiM1MWeWolWyWrpTbyzr35_FYHrHfBhGtX_6QhWGxOqaEK1NaGavM62DfkBHjp7kA</recordid><startdate>201712</startdate><enddate>201712</enddate><creator>Fujita, T.</creator><creator>Sofue, T.</creator><creator>Moritoki, M.</creator><creator>Nishijima, Y.</creator><creator>Tokuhara, Y.</creator><creator>Wakisaka, H.</creator><creator>Kushida, Y.</creator><creator>Haba, R.</creator><creator>Ohsaki, H.</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QP</scope><scope>7TK</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6046-3785</orcidid></search><sort><creationdate>201712</creationdate><title>Urinary WT1‐positive cells as a non‐invasive biomarker of crescent formation</title><author>Fujita, T. ; Sofue, T. ; Moritoki, M. ; Nishijima, Y. ; Tokuhara, Y. ; Wakisaka, H. ; Kushida, Y. ; Haba, R. ; Ohsaki, H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j4240-fd879a7178d633efcfe813cfb2ba43cdcbed2d13d8df91b34944bd0d793afbd93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adult</topic><topic>Biomarkers - urine</topic><topic>Biopsy</topic><topic>crescent formation</topic><topic>Cytology</topic><topic>Epithelial cells</topic><topic>Female</topic><topic>Humans</topic><topic>immunocytochemistry</topic><topic>Keratins - urine</topic><topic>Kidney Diseases - diagnosis</topic><topic>Kidney Diseases - urine</topic><topic>Kidney transplantation</topic><topic>liquid‐based cytology</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Podocytes - pathology</topic><topic>Studies</topic><topic>Urine</topic><topic>urine cytology</topic><topic>WT1</topic><topic>WT1 Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fujita, T.</creatorcontrib><creatorcontrib>Sofue, T.</creatorcontrib><creatorcontrib>Moritoki, M.</creatorcontrib><creatorcontrib>Nishijima, Y.</creatorcontrib><creatorcontrib>Tokuhara, Y.</creatorcontrib><creatorcontrib>Wakisaka, H.</creatorcontrib><creatorcontrib>Kushida, Y.</creatorcontrib><creatorcontrib>Haba, R.</creatorcontrib><creatorcontrib>Ohsaki, H.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cytopathology (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fujita, T.</au><au>Sofue, T.</au><au>Moritoki, M.</au><au>Nishijima, Y.</au><au>Tokuhara, Y.</au><au>Wakisaka, H.</au><au>Kushida, Y.</au><au>Haba, R.</au><au>Ohsaki, H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Urinary WT1‐positive cells as a non‐invasive biomarker of crescent formation</atitle><jtitle>Cytopathology (Oxford)</jtitle><addtitle>Cytopathology</addtitle><date>2017-12</date><risdate>2017</risdate><volume>28</volume><issue>6</issue><spage>524</spage><epage>530</epage><pages>524-530</pages><issn>0956-5507</issn><eissn>1365-2303</eissn><abstract>Objective
The purpose of this study was to assess the relationship between urinary WT1‐positive cells (podocytes and active parietal epithelial cells) and WT1‐positive cells in renal biopsy to investigate whether urinary WT1‐positive cells are useful for detection of crescent formation.
Methods
Fifty‐two patients with kidney disease were investigated (15 cases with crescentic lesions and 37 cases with non‐crescentic lesions) for immunoenzyme staining using anti‐WT1 antibody for urine cytology and renal biopsy. Numbers of WT1‐positive cells in urine and renal biopsy were counted.
Results
There was no correlation between urinary WT1‐positive cells and WT1‐positive cells in renal biopsy. However, the number of urinary WT1‐positive cells in patients with crescentic lesions was significantly higher than in patients with non‐crescentic lesions. In addition, the best cut‐off value to detect patients with crescentic lesions using urinary was 5 cells/10‐mL (area under the concentration‐time curve=0.735).
Conclusions
The results of our study suggest urinary WT1‐positive cells can be used to detect patients with crescent formation using 5 cells/10‐mL cutoff value. WT1‐positive glomerular podocytes and parietal epithelial cells may be shed into urine in active glomerular disease. This study, investigating the relationship between WT1‐positive cells in urine and in the renal biopsy found no correlation; however, the results do suggest that, using a cutoff value of 5 cells/10 mL, WT1 positive urinary cells can be used to detect patients with crescent formation.
WT1 positive glomerular podocytes and parietal epithelial cells may be shed into urine in active glomerular disease. This study, investigating the relationship between WT1 positive cells in urine and in the renal biopsy found no correlation; however, the results do suggest that, using a cutoff value of 5 cells/10 mL, WT1 positive urinary cells can be used to detect patients with crescentic formation.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>28913845</pmid><doi>10.1111/cyt.12460</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0001-6046-3785</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0956-5507 |
| ispartof | Cytopathology (Oxford), 2017-12, Vol.28 (6), p.524-530 |
| issn | 0956-5507 1365-2303 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1940059027 |
| source | MEDLINE; Wiley Journals |
| subjects | Adult Biomarkers - urine Biopsy crescent formation Cytology Epithelial cells Female Humans immunocytochemistry Keratins - urine Kidney Diseases - diagnosis Kidney Diseases - urine Kidney transplantation liquid‐based cytology Male Middle Aged Podocytes - pathology Studies Urine urine cytology WT1 WT1 Proteins - metabolism |
| title | Urinary WT1‐positive cells as a non‐invasive biomarker of crescent formation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T07%3A46%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Urinary%20WT1%E2%80%90positive%20cells%20as%20a%20non%E2%80%90invasive%20biomarker%20of%20crescent%20formation&rft.jtitle=Cytopathology%20(Oxford)&rft.au=Fujita,%20T.&rft.date=2017-12&rft.volume=28&rft.issue=6&rft.spage=524&rft.epage=530&rft.pages=524-530&rft.issn=0956-5507&rft.eissn=1365-2303&rft_id=info:doi/10.1111/cyt.12460&rft_dat=%3Cproquest_pubme%3E1966847190%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1966847190&rft_id=info:pmid/28913845&rfr_iscdi=true |