Autographa californica multiple nucleopolyhedrovirus PK1 is a factor that regulates high-level expression of very late genes in viral infection

The remarkable ability of baculovirus is to hyperexpress very late genes, but the mechanisms remain unclear. Here we report the effect of PK1, a baculovirus-encoded serine/threonine kinase, on very late gene hyperexpression. PK1 knockout does not completely disrupt very late gene expression, but dow...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Virology (New York, N.Y.) N.Y.), 2017-12, Vol.512, p.56-65
Hauptverfasser: Liang, Changyong, Su, Xia, Xu, Guodong, Dai, Xuejuan, Zhao, Shuling
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 65
container_issue
container_start_page 56
container_title Virology (New York, N.Y.)
container_volume 512
creator Liang, Changyong
Su, Xia
Xu, Guodong
Dai, Xuejuan
Zhao, Shuling
description The remarkable ability of baculovirus is to hyperexpress very late genes, but the mechanisms remain unclear. Here we report the effect of PK1, a baculovirus-encoded serine/threonine kinase, on very late gene hyperexpression. PK1 knockout does not completely disrupt very late gene expression, but down regulates the hyperexpression. Those truncated PK1s that exhibit kinase activity in vitro rescue the decline of very late hyperexpression, while other truncated PK1s and a point mutant PK1 (D137A) without kinase activity fail to rescue the decline of very late hyperexpression, suggesting that PK1 regulates very late gene expression by its kinase activity. In addition, those PK1 mutants that can rescue the hyperexpression are able to interact with very late promoter containing 5′ UTR. Based on the above data, we hypothesize that PK1 binds to very late promoter containing 5′ UTR to regulate the hyperexpression of very late genes by its kinase activity. •PK1 knockout does not completely disrupt very late gene expression.•PK1 knockout down regulates very late gene hyperexpression.•The catalytic domain of PK1 is essential to very late gene hyperexpression.•PK1 interacts with very late promoter with 5′ UTR containing burst sequence.•Kinase activity of PK1 is involved in very late hyperexpression and infectivity.
doi_str_mv 10.1016/j.virol.2017.09.004
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1940054582</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0042682217303057</els_id><sourcerecordid>1940054582</sourcerecordid><originalsourceid>FETCH-LOGICAL-c404t-8c4b4ff5b565d24727bc5a1fafef98d24f8ae9148958d51e47b17dc5cb6ede943</originalsourceid><addsrcrecordid>eNp9kcFu3CAQhlGVqNmkfYJKFcdc7IIXbHPoIVqlbZRIySE5I4yHNSvWuIBX2afIK4ftbnvsaZjRN_OL_0foCyUlJbT-til3NnhXVoQ2JRElIewDWlAi6oIsGT1Dizypirqtqgt0GeOG5L5pyEd0UbWCckbqBXq7mZNfBzUNCmvlrPFhtFrh7eySnRzgcdYO_OTdfoA--Cw5R_x0T7GNWGGjdPIBp0ElHGA9O5Ug4sGuh8LBDhyG1ylAjNaP2Bu8g7DHBwavYcygHXE-qFx-GNApU5_QuVEuwudTvUIvP26fV7-Kh8efd6ubh0IzwlLRatYxY3jHa95XrKmaTnNFjTJgRJsnplUgKGsFb3tOgTUdbXrNdVdDD4Itr9D18e4U_O8ZYpJbGzU4p0bwc5RUMEI4422V0eUR1cHHGMDIKditCntJiTwkITfyTxLykIQkQmaf89bXk8DcbaH_t_PX-gx8PwKQv7mzEGTUFkYNvQ3ZC9l7-1-BdwIpn1s</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1940054582</pqid></control><display><type>article</type><title>Autographa californica multiple nucleopolyhedrovirus PK1 is a factor that regulates high-level expression of very late genes in viral infection</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Liang, Changyong ; Su, Xia ; Xu, Guodong ; Dai, Xuejuan ; Zhao, Shuling</creator><creatorcontrib>Liang, Changyong ; Su, Xia ; Xu, Guodong ; Dai, Xuejuan ; Zhao, Shuling</creatorcontrib><description>The remarkable ability of baculovirus is to hyperexpress very late genes, but the mechanisms remain unclear. Here we report the effect of PK1, a baculovirus-encoded serine/threonine kinase, on very late gene hyperexpression. PK1 knockout does not completely disrupt very late gene expression, but down regulates the hyperexpression. Those truncated PK1s that exhibit kinase activity in vitro rescue the decline of very late hyperexpression, while other truncated PK1s and a point mutant PK1 (D137A) without kinase activity fail to rescue the decline of very late hyperexpression, suggesting that PK1 regulates very late gene expression by its kinase activity. In addition, those PK1 mutants that can rescue the hyperexpression are able to interact with very late promoter containing 5′ UTR. Based on the above data, we hypothesize that PK1 binds to very late promoter containing 5′ UTR to regulate the hyperexpression of very late genes by its kinase activity. •PK1 knockout does not completely disrupt very late gene expression.•PK1 knockout down regulates very late gene hyperexpression.•The catalytic domain of PK1 is essential to very late gene hyperexpression.•PK1 interacts with very late promoter with 5′ UTR containing burst sequence.•Kinase activity of PK1 is involved in very late hyperexpression and infectivity.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1016/j.virol.2017.09.004</identifier><identifier>PMID: 28915406</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>AcMNPV ; Activity of kinase ; Animals ; Baculovirus ; Burst sequence ; Gene Expression Regulation, Enzymologic - physiology ; Gene Expression Regulation, Viral - physiology ; Nucleopolyhedrovirus - classification ; Nucleopolyhedrovirus - genetics ; Nucleopolyhedrovirus - metabolism ; PK1 ; Protein-Serine-Threonine Kinases - genetics ; Protein-Serine-Threonine Kinases - metabolism ; Sf9 Cells ; Very late gene hyperexpression ; Very late promoter ; Viral Proteins - genetics ; Viral Proteins - metabolism ; Virus Replication - physiology</subject><ispartof>Virology (New York, N.Y.), 2017-12, Vol.512, p.56-65</ispartof><rights>2017 Elsevier Inc.</rights><rights>Copyright © 2017 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c404t-8c4b4ff5b565d24727bc5a1fafef98d24f8ae9148958d51e47b17dc5cb6ede943</citedby><cites>FETCH-LOGICAL-c404t-8c4b4ff5b565d24727bc5a1fafef98d24f8ae9148958d51e47b17dc5cb6ede943</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.virol.2017.09.004$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28915406$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liang, Changyong</creatorcontrib><creatorcontrib>Su, Xia</creatorcontrib><creatorcontrib>Xu, Guodong</creatorcontrib><creatorcontrib>Dai, Xuejuan</creatorcontrib><creatorcontrib>Zhao, Shuling</creatorcontrib><title>Autographa californica multiple nucleopolyhedrovirus PK1 is a factor that regulates high-level expression of very late genes in viral infection</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>The remarkable ability of baculovirus is to hyperexpress very late genes, but the mechanisms remain unclear. Here we report the effect of PK1, a baculovirus-encoded serine/threonine kinase, on very late gene hyperexpression. PK1 knockout does not completely disrupt very late gene expression, but down regulates the hyperexpression. Those truncated PK1s that exhibit kinase activity in vitro rescue the decline of very late hyperexpression, while other truncated PK1s and a point mutant PK1 (D137A) without kinase activity fail to rescue the decline of very late hyperexpression, suggesting that PK1 regulates very late gene expression by its kinase activity. In addition, those PK1 mutants that can rescue the hyperexpression are able to interact with very late promoter containing 5′ UTR. Based on the above data, we hypothesize that PK1 binds to very late promoter containing 5′ UTR to regulate the hyperexpression of very late genes by its kinase activity. •PK1 knockout does not completely disrupt very late gene expression.•PK1 knockout down regulates very late gene hyperexpression.•The catalytic domain of PK1 is essential to very late gene hyperexpression.•PK1 interacts with very late promoter with 5′ UTR containing burst sequence.•Kinase activity of PK1 is involved in very late hyperexpression and infectivity.</description><subject>AcMNPV</subject><subject>Activity of kinase</subject><subject>Animals</subject><subject>Baculovirus</subject><subject>Burst sequence</subject><subject>Gene Expression Regulation, Enzymologic - physiology</subject><subject>Gene Expression Regulation, Viral - physiology</subject><subject>Nucleopolyhedrovirus - classification</subject><subject>Nucleopolyhedrovirus - genetics</subject><subject>Nucleopolyhedrovirus - metabolism</subject><subject>PK1</subject><subject>Protein-Serine-Threonine Kinases - genetics</subject><subject>Protein-Serine-Threonine Kinases - metabolism</subject><subject>Sf9 Cells</subject><subject>Very late gene hyperexpression</subject><subject>Very late promoter</subject><subject>Viral Proteins - genetics</subject><subject>Viral Proteins - metabolism</subject><subject>Virus Replication - physiology</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFu3CAQhlGVqNmkfYJKFcdc7IIXbHPoIVqlbZRIySE5I4yHNSvWuIBX2afIK4ftbnvsaZjRN_OL_0foCyUlJbT-til3NnhXVoQ2JRElIewDWlAi6oIsGT1Dizypirqtqgt0GeOG5L5pyEd0UbWCckbqBXq7mZNfBzUNCmvlrPFhtFrh7eySnRzgcdYO_OTdfoA--Cw5R_x0T7GNWGGjdPIBp0ElHGA9O5Ug4sGuh8LBDhyG1ylAjNaP2Bu8g7DHBwavYcygHXE-qFx-GNApU5_QuVEuwudTvUIvP26fV7-Kh8efd6ubh0IzwlLRatYxY3jHa95XrKmaTnNFjTJgRJsnplUgKGsFb3tOgTUdbXrNdVdDD4Itr9D18e4U_O8ZYpJbGzU4p0bwc5RUMEI4422V0eUR1cHHGMDIKditCntJiTwkITfyTxLykIQkQmaf89bXk8DcbaH_t_PX-gx8PwKQv7mzEGTUFkYNvQ3ZC9l7-1-BdwIpn1s</recordid><startdate>201712</startdate><enddate>201712</enddate><creator>Liang, Changyong</creator><creator>Su, Xia</creator><creator>Xu, Guodong</creator><creator>Dai, Xuejuan</creator><creator>Zhao, Shuling</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201712</creationdate><title>Autographa californica multiple nucleopolyhedrovirus PK1 is a factor that regulates high-level expression of very late genes in viral infection</title><author>Liang, Changyong ; Su, Xia ; Xu, Guodong ; Dai, Xuejuan ; Zhao, Shuling</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c404t-8c4b4ff5b565d24727bc5a1fafef98d24f8ae9148958d51e47b17dc5cb6ede943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>AcMNPV</topic><topic>Activity of kinase</topic><topic>Animals</topic><topic>Baculovirus</topic><topic>Burst sequence</topic><topic>Gene Expression Regulation, Enzymologic - physiology</topic><topic>Gene Expression Regulation, Viral - physiology</topic><topic>Nucleopolyhedrovirus - classification</topic><topic>Nucleopolyhedrovirus - genetics</topic><topic>Nucleopolyhedrovirus - metabolism</topic><topic>PK1</topic><topic>Protein-Serine-Threonine Kinases - genetics</topic><topic>Protein-Serine-Threonine Kinases - metabolism</topic><topic>Sf9 Cells</topic><topic>Very late gene hyperexpression</topic><topic>Very late promoter</topic><topic>Viral Proteins - genetics</topic><topic>Viral Proteins - metabolism</topic><topic>Virus Replication - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liang, Changyong</creatorcontrib><creatorcontrib>Su, Xia</creatorcontrib><creatorcontrib>Xu, Guodong</creatorcontrib><creatorcontrib>Dai, Xuejuan</creatorcontrib><creatorcontrib>Zhao, Shuling</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liang, Changyong</au><au>Su, Xia</au><au>Xu, Guodong</au><au>Dai, Xuejuan</au><au>Zhao, Shuling</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Autographa californica multiple nucleopolyhedrovirus PK1 is a factor that regulates high-level expression of very late genes in viral infection</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>2017-12</date><risdate>2017</risdate><volume>512</volume><spage>56</spage><epage>65</epage><pages>56-65</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><abstract>The remarkable ability of baculovirus is to hyperexpress very late genes, but the mechanisms remain unclear. Here we report the effect of PK1, a baculovirus-encoded serine/threonine kinase, on very late gene hyperexpression. PK1 knockout does not completely disrupt very late gene expression, but down regulates the hyperexpression. Those truncated PK1s that exhibit kinase activity in vitro rescue the decline of very late hyperexpression, while other truncated PK1s and a point mutant PK1 (D137A) without kinase activity fail to rescue the decline of very late hyperexpression, suggesting that PK1 regulates very late gene expression by its kinase activity. In addition, those PK1 mutants that can rescue the hyperexpression are able to interact with very late promoter containing 5′ UTR. Based on the above data, we hypothesize that PK1 binds to very late promoter containing 5′ UTR to regulate the hyperexpression of very late genes by its kinase activity. •PK1 knockout does not completely disrupt very late gene expression.•PK1 knockout down regulates very late gene hyperexpression.•The catalytic domain of PK1 is essential to very late gene hyperexpression.•PK1 interacts with very late promoter with 5′ UTR containing burst sequence.•Kinase activity of PK1 is involved in very late hyperexpression and infectivity.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>28915406</pmid><doi>10.1016/j.virol.2017.09.004</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0042-6822
ispartof Virology (New York, N.Y.), 2017-12, Vol.512, p.56-65
issn 0042-6822
1096-0341
language eng
recordid cdi_proquest_miscellaneous_1940054582
source MEDLINE; Elsevier ScienceDirect Journals Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals
subjects AcMNPV
Activity of kinase
Animals
Baculovirus
Burst sequence
Gene Expression Regulation, Enzymologic - physiology
Gene Expression Regulation, Viral - physiology
Nucleopolyhedrovirus - classification
Nucleopolyhedrovirus - genetics
Nucleopolyhedrovirus - metabolism
PK1
Protein-Serine-Threonine Kinases - genetics
Protein-Serine-Threonine Kinases - metabolism
Sf9 Cells
Very late gene hyperexpression
Very late promoter
Viral Proteins - genetics
Viral Proteins - metabolism
Virus Replication - physiology
title Autographa californica multiple nucleopolyhedrovirus PK1 is a factor that regulates high-level expression of very late genes in viral infection
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T23%3A53%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Autographa%20californica%20multiple%20nucleopolyhedrovirus%20PK1%20is%20a%20factor%20that%20regulates%20high-level%20expression%20of%20very%20late%20genes%20in%20viral%20infection&rft.jtitle=Virology%20(New%20York,%20N.Y.)&rft.au=Liang,%20Changyong&rft.date=2017-12&rft.volume=512&rft.spage=56&rft.epage=65&rft.pages=56-65&rft.issn=0042-6822&rft.eissn=1096-0341&rft_id=info:doi/10.1016/j.virol.2017.09.004&rft_dat=%3Cproquest_cross%3E1940054582%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1940054582&rft_id=info:pmid/28915406&rft_els_id=S0042682217303057&rfr_iscdi=true