Autographa californica multiple nucleopolyhedrovirus PK1 is a factor that regulates high-level expression of very late genes in viral infection
The remarkable ability of baculovirus is to hyperexpress very late genes, but the mechanisms remain unclear. Here we report the effect of PK1, a baculovirus-encoded serine/threonine kinase, on very late gene hyperexpression. PK1 knockout does not completely disrupt very late gene expression, but dow...
Gespeichert in:
Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 2017-12, Vol.512, p.56-65 |
---|---|
Hauptverfasser: | , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 65 |
---|---|
container_issue | |
container_start_page | 56 |
container_title | Virology (New York, N.Y.) |
container_volume | 512 |
creator | Liang, Changyong Su, Xia Xu, Guodong Dai, Xuejuan Zhao, Shuling |
description | The remarkable ability of baculovirus is to hyperexpress very late genes, but the mechanisms remain unclear. Here we report the effect of PK1, a baculovirus-encoded serine/threonine kinase, on very late gene hyperexpression. PK1 knockout does not completely disrupt very late gene expression, but down regulates the hyperexpression. Those truncated PK1s that exhibit kinase activity in vitro rescue the decline of very late hyperexpression, while other truncated PK1s and a point mutant PK1 (D137A) without kinase activity fail to rescue the decline of very late hyperexpression, suggesting that PK1 regulates very late gene expression by its kinase activity. In addition, those PK1 mutants that can rescue the hyperexpression are able to interact with very late promoter containing 5′ UTR. Based on the above data, we hypothesize that PK1 binds to very late promoter containing 5′ UTR to regulate the hyperexpression of very late genes by its kinase activity.
•PK1 knockout does not completely disrupt very late gene expression.•PK1 knockout down regulates very late gene hyperexpression.•The catalytic domain of PK1 is essential to very late gene hyperexpression.•PK1 interacts with very late promoter with 5′ UTR containing burst sequence.•Kinase activity of PK1 is involved in very late hyperexpression and infectivity. |
doi_str_mv | 10.1016/j.virol.2017.09.004 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1940054582</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0042682217303057</els_id><sourcerecordid>1940054582</sourcerecordid><originalsourceid>FETCH-LOGICAL-c404t-8c4b4ff5b565d24727bc5a1fafef98d24f8ae9148958d51e47b17dc5cb6ede943</originalsourceid><addsrcrecordid>eNp9kcFu3CAQhlGVqNmkfYJKFcdc7IIXbHPoIVqlbZRIySE5I4yHNSvWuIBX2afIK4ftbnvsaZjRN_OL_0foCyUlJbT-til3NnhXVoQ2JRElIewDWlAi6oIsGT1Dizypirqtqgt0GeOG5L5pyEd0UbWCckbqBXq7mZNfBzUNCmvlrPFhtFrh7eySnRzgcdYO_OTdfoA--Cw5R_x0T7GNWGGjdPIBp0ElHGA9O5Ug4sGuh8LBDhyG1ylAjNaP2Bu8g7DHBwavYcygHXE-qFx-GNApU5_QuVEuwudTvUIvP26fV7-Kh8efd6ubh0IzwlLRatYxY3jHa95XrKmaTnNFjTJgRJsnplUgKGsFb3tOgTUdbXrNdVdDD4Itr9D18e4U_O8ZYpJbGzU4p0bwc5RUMEI4422V0eUR1cHHGMDIKditCntJiTwkITfyTxLykIQkQmaf89bXk8DcbaH_t_PX-gx8PwKQv7mzEGTUFkYNvQ3ZC9l7-1-BdwIpn1s</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1940054582</pqid></control><display><type>article</type><title>Autographa californica multiple nucleopolyhedrovirus PK1 is a factor that regulates high-level expression of very late genes in viral infection</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Liang, Changyong ; Su, Xia ; Xu, Guodong ; Dai, Xuejuan ; Zhao, Shuling</creator><creatorcontrib>Liang, Changyong ; Su, Xia ; Xu, Guodong ; Dai, Xuejuan ; Zhao, Shuling</creatorcontrib><description>The remarkable ability of baculovirus is to hyperexpress very late genes, but the mechanisms remain unclear. Here we report the effect of PK1, a baculovirus-encoded serine/threonine kinase, on very late gene hyperexpression. PK1 knockout does not completely disrupt very late gene expression, but down regulates the hyperexpression. Those truncated PK1s that exhibit kinase activity in vitro rescue the decline of very late hyperexpression, while other truncated PK1s and a point mutant PK1 (D137A) without kinase activity fail to rescue the decline of very late hyperexpression, suggesting that PK1 regulates very late gene expression by its kinase activity. In addition, those PK1 mutants that can rescue the hyperexpression are able to interact with very late promoter containing 5′ UTR. Based on the above data, we hypothesize that PK1 binds to very late promoter containing 5′ UTR to regulate the hyperexpression of very late genes by its kinase activity.
•PK1 knockout does not completely disrupt very late gene expression.•PK1 knockout down regulates very late gene hyperexpression.•The catalytic domain of PK1 is essential to very late gene hyperexpression.•PK1 interacts with very late promoter with 5′ UTR containing burst sequence.•Kinase activity of PK1 is involved in very late hyperexpression and infectivity.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1016/j.virol.2017.09.004</identifier><identifier>PMID: 28915406</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>AcMNPV ; Activity of kinase ; Animals ; Baculovirus ; Burst sequence ; Gene Expression Regulation, Enzymologic - physiology ; Gene Expression Regulation, Viral - physiology ; Nucleopolyhedrovirus - classification ; Nucleopolyhedrovirus - genetics ; Nucleopolyhedrovirus - metabolism ; PK1 ; Protein-Serine-Threonine Kinases - genetics ; Protein-Serine-Threonine Kinases - metabolism ; Sf9 Cells ; Very late gene hyperexpression ; Very late promoter ; Viral Proteins - genetics ; Viral Proteins - metabolism ; Virus Replication - physiology</subject><ispartof>Virology (New York, N.Y.), 2017-12, Vol.512, p.56-65</ispartof><rights>2017 Elsevier Inc.</rights><rights>Copyright © 2017 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c404t-8c4b4ff5b565d24727bc5a1fafef98d24f8ae9148958d51e47b17dc5cb6ede943</citedby><cites>FETCH-LOGICAL-c404t-8c4b4ff5b565d24727bc5a1fafef98d24f8ae9148958d51e47b17dc5cb6ede943</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.virol.2017.09.004$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28915406$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liang, Changyong</creatorcontrib><creatorcontrib>Su, Xia</creatorcontrib><creatorcontrib>Xu, Guodong</creatorcontrib><creatorcontrib>Dai, Xuejuan</creatorcontrib><creatorcontrib>Zhao, Shuling</creatorcontrib><title>Autographa californica multiple nucleopolyhedrovirus PK1 is a factor that regulates high-level expression of very late genes in viral infection</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>The remarkable ability of baculovirus is to hyperexpress very late genes, but the mechanisms remain unclear. Here we report the effect of PK1, a baculovirus-encoded serine/threonine kinase, on very late gene hyperexpression. PK1 knockout does not completely disrupt very late gene expression, but down regulates the hyperexpression. Those truncated PK1s that exhibit kinase activity in vitro rescue the decline of very late hyperexpression, while other truncated PK1s and a point mutant PK1 (D137A) without kinase activity fail to rescue the decline of very late hyperexpression, suggesting that PK1 regulates very late gene expression by its kinase activity. In addition, those PK1 mutants that can rescue the hyperexpression are able to interact with very late promoter containing 5′ UTR. Based on the above data, we hypothesize that PK1 binds to very late promoter containing 5′ UTR to regulate the hyperexpression of very late genes by its kinase activity.
•PK1 knockout does not completely disrupt very late gene expression.•PK1 knockout down regulates very late gene hyperexpression.•The catalytic domain of PK1 is essential to very late gene hyperexpression.•PK1 interacts with very late promoter with 5′ UTR containing burst sequence.•Kinase activity of PK1 is involved in very late hyperexpression and infectivity.</description><subject>AcMNPV</subject><subject>Activity of kinase</subject><subject>Animals</subject><subject>Baculovirus</subject><subject>Burst sequence</subject><subject>Gene Expression Regulation, Enzymologic - physiology</subject><subject>Gene Expression Regulation, Viral - physiology</subject><subject>Nucleopolyhedrovirus - classification</subject><subject>Nucleopolyhedrovirus - genetics</subject><subject>Nucleopolyhedrovirus - metabolism</subject><subject>PK1</subject><subject>Protein-Serine-Threonine Kinases - genetics</subject><subject>Protein-Serine-Threonine Kinases - metabolism</subject><subject>Sf9 Cells</subject><subject>Very late gene hyperexpression</subject><subject>Very late promoter</subject><subject>Viral Proteins - genetics</subject><subject>Viral Proteins - metabolism</subject><subject>Virus Replication - physiology</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFu3CAQhlGVqNmkfYJKFcdc7IIXbHPoIVqlbZRIySE5I4yHNSvWuIBX2afIK4ftbnvsaZjRN_OL_0foCyUlJbT-til3NnhXVoQ2JRElIewDWlAi6oIsGT1Dizypirqtqgt0GeOG5L5pyEd0UbWCckbqBXq7mZNfBzUNCmvlrPFhtFrh7eySnRzgcdYO_OTdfoA--Cw5R_x0T7GNWGGjdPIBp0ElHGA9O5Ug4sGuh8LBDhyG1ylAjNaP2Bu8g7DHBwavYcygHXE-qFx-GNApU5_QuVEuwudTvUIvP26fV7-Kh8efd6ubh0IzwlLRatYxY3jHa95XrKmaTnNFjTJgRJsnplUgKGsFb3tOgTUdbXrNdVdDD4Itr9D18e4U_O8ZYpJbGzU4p0bwc5RUMEI4422V0eUR1cHHGMDIKditCntJiTwkITfyTxLykIQkQmaf89bXk8DcbaH_t_PX-gx8PwKQv7mzEGTUFkYNvQ3ZC9l7-1-BdwIpn1s</recordid><startdate>201712</startdate><enddate>201712</enddate><creator>Liang, Changyong</creator><creator>Su, Xia</creator><creator>Xu, Guodong</creator><creator>Dai, Xuejuan</creator><creator>Zhao, Shuling</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201712</creationdate><title>Autographa californica multiple nucleopolyhedrovirus PK1 is a factor that regulates high-level expression of very late genes in viral infection</title><author>Liang, Changyong ; Su, Xia ; Xu, Guodong ; Dai, Xuejuan ; Zhao, Shuling</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c404t-8c4b4ff5b565d24727bc5a1fafef98d24f8ae9148958d51e47b17dc5cb6ede943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>AcMNPV</topic><topic>Activity of kinase</topic><topic>Animals</topic><topic>Baculovirus</topic><topic>Burst sequence</topic><topic>Gene Expression Regulation, Enzymologic - physiology</topic><topic>Gene Expression Regulation, Viral - physiology</topic><topic>Nucleopolyhedrovirus - classification</topic><topic>Nucleopolyhedrovirus - genetics</topic><topic>Nucleopolyhedrovirus - metabolism</topic><topic>PK1</topic><topic>Protein-Serine-Threonine Kinases - genetics</topic><topic>Protein-Serine-Threonine Kinases - metabolism</topic><topic>Sf9 Cells</topic><topic>Very late gene hyperexpression</topic><topic>Very late promoter</topic><topic>Viral Proteins - genetics</topic><topic>Viral Proteins - metabolism</topic><topic>Virus Replication - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liang, Changyong</creatorcontrib><creatorcontrib>Su, Xia</creatorcontrib><creatorcontrib>Xu, Guodong</creatorcontrib><creatorcontrib>Dai, Xuejuan</creatorcontrib><creatorcontrib>Zhao, Shuling</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liang, Changyong</au><au>Su, Xia</au><au>Xu, Guodong</au><au>Dai, Xuejuan</au><au>Zhao, Shuling</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Autographa californica multiple nucleopolyhedrovirus PK1 is a factor that regulates high-level expression of very late genes in viral infection</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>2017-12</date><risdate>2017</risdate><volume>512</volume><spage>56</spage><epage>65</epage><pages>56-65</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><abstract>The remarkable ability of baculovirus is to hyperexpress very late genes, but the mechanisms remain unclear. Here we report the effect of PK1, a baculovirus-encoded serine/threonine kinase, on very late gene hyperexpression. PK1 knockout does not completely disrupt very late gene expression, but down regulates the hyperexpression. Those truncated PK1s that exhibit kinase activity in vitro rescue the decline of very late hyperexpression, while other truncated PK1s and a point mutant PK1 (D137A) without kinase activity fail to rescue the decline of very late hyperexpression, suggesting that PK1 regulates very late gene expression by its kinase activity. In addition, those PK1 mutants that can rescue the hyperexpression are able to interact with very late promoter containing 5′ UTR. Based on the above data, we hypothesize that PK1 binds to very late promoter containing 5′ UTR to regulate the hyperexpression of very late genes by its kinase activity.
•PK1 knockout does not completely disrupt very late gene expression.•PK1 knockout down regulates very late gene hyperexpression.•The catalytic domain of PK1 is essential to very late gene hyperexpression.•PK1 interacts with very late promoter with 5′ UTR containing burst sequence.•Kinase activity of PK1 is involved in very late hyperexpression and infectivity.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>28915406</pmid><doi>10.1016/j.virol.2017.09.004</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0042-6822 |
ispartof | Virology (New York, N.Y.), 2017-12, Vol.512, p.56-65 |
issn | 0042-6822 1096-0341 |
language | eng |
recordid | cdi_proquest_miscellaneous_1940054582 |
source | MEDLINE; Elsevier ScienceDirect Journals Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
subjects | AcMNPV Activity of kinase Animals Baculovirus Burst sequence Gene Expression Regulation, Enzymologic - physiology Gene Expression Regulation, Viral - physiology Nucleopolyhedrovirus - classification Nucleopolyhedrovirus - genetics Nucleopolyhedrovirus - metabolism PK1 Protein-Serine-Threonine Kinases - genetics Protein-Serine-Threonine Kinases - metabolism Sf9 Cells Very late gene hyperexpression Very late promoter Viral Proteins - genetics Viral Proteins - metabolism Virus Replication - physiology |
title | Autographa californica multiple nucleopolyhedrovirus PK1 is a factor that regulates high-level expression of very late genes in viral infection |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T23%3A53%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Autographa%20californica%20multiple%20nucleopolyhedrovirus%20PK1%20is%20a%20factor%20that%20regulates%20high-level%20expression%20of%20very%20late%20genes%20in%20viral%20infection&rft.jtitle=Virology%20(New%20York,%20N.Y.)&rft.au=Liang,%20Changyong&rft.date=2017-12&rft.volume=512&rft.spage=56&rft.epage=65&rft.pages=56-65&rft.issn=0042-6822&rft.eissn=1096-0341&rft_id=info:doi/10.1016/j.virol.2017.09.004&rft_dat=%3Cproquest_cross%3E1940054582%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1940054582&rft_id=info:pmid/28915406&rft_els_id=S0042682217303057&rfr_iscdi=true |