Osmoprotection of Salmonella enterica serovar Typhimurium by N γ -acetyldiaminobutyrate, the precursor of the compatible solute ectoine
N γ -acetyl-2,4-diaminobutyrate (NADA), the precursor of the compatible solute ectoine, was shown to function as an osmoprotectant for the non-halophilic bacterium Salmonella enterica serovar Typhimurium. The addition of NADA-containing extracts of an ectoine synthase mutant of the broad salt-growin...
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| Veröffentlicht in: | Systematic and applied microbiology 2006-12, Vol.29 (8), p.626-633 |
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| container_title | Systematic and applied microbiology |
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| creator | García-Estepa, Raul Cánovas, David Iglesias-Guerra, Fernando Ventosa, Antonio Csonka, Laszlo N. Nieto, Joaquín J. Vargas, Carmen |
| description | N
γ
-acetyl-2,4-diaminobutyrate (NADA), the precursor of the compatible solute ectoine, was shown to function as an osmoprotectant for the non-halophilic bacterium
Salmonella enterica serovar Typhimurium. The addition of NADA-containing extracts of an ectoine synthase mutant of the broad salt-growing halophile
Chromohalobacter salexigens DSM 3043
T could alleviate the inhibitory effects of high salinity in
S. enterica, which lacks the ectoine biosynthetic pathway. NADA, purified from extracts of the mutant, protected
S. enterica against salinity stress. This osmoprotective effect was slightly lower than that of ectoine, but more potent than that of hydroxyectoine. Accumulation of purified NADA by
S. enterica was demonstrated by
13C-NMR spectroscopy and HPLC analysis. In addition, it was shown that NADA was taken up by
S. enterica via the ProP and ProU transport systems, which are known to transport glycine betaine and proline. This finding provides evidence that these permeases can recognize a diaminoacid that carries an unsubstituted
α
-amino group. This is the first time that NADA has been connected with osmoprotective functions in non-halophilic bacteria. |
| doi_str_mv | 10.1016/j.syapm.2006.01.008 |
| format | Article |
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γ
-acetyl-2,4-diaminobutyrate (NADA), the precursor of the compatible solute ectoine, was shown to function as an osmoprotectant for the non-halophilic bacterium
Salmonella enterica serovar Typhimurium. The addition of NADA-containing extracts of an ectoine synthase mutant of the broad salt-growing halophile
Chromohalobacter salexigens DSM 3043
T could alleviate the inhibitory effects of high salinity in
S. enterica, which lacks the ectoine biosynthetic pathway. NADA, purified from extracts of the mutant, protected
S. enterica against salinity stress. This osmoprotective effect was slightly lower than that of ectoine, but more potent than that of hydroxyectoine. Accumulation of purified NADA by
S. enterica was demonstrated by
13C-NMR spectroscopy and HPLC analysis. In addition, it was shown that NADA was taken up by
S. enterica via the ProP and ProU transport systems, which are known to transport glycine betaine and proline. This finding provides evidence that these permeases can recognize a diaminoacid that carries an unsubstituted
α
-amino group. This is the first time that NADA has been connected with osmoprotective functions in non-halophilic bacteria.</description><identifier>ISSN: 0723-2020</identifier><identifier>EISSN: 1618-0984</identifier><identifier>DOI: 10.1016/j.syapm.2006.01.008</identifier><identifier>CODEN: SAMIDF</identifier><language>eng</language><publisher>Jena: Elsevier GmbH</publisher><subject>Bacteriology ; Biological and medical sciences ; Chromohalobacter salexigens ; Compatible solutes ; Ectoine ; Fundamental and applied biological sciences. Psychology ; Hydroxyectoine ; Microbiology ; Miscellaneous ; N γ-acetyldiaminobutyrate ; Osmoregulation ; Salmonella enterica</subject><ispartof>Systematic and applied microbiology, 2006-12, Vol.29 (8), p.626-633</ispartof><rights>2006 Elsevier GmbH</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c364t-b55640592a679e50b72ea8b3bb4ad19cb9b35816fc5bd3dac5f296413ccee3d93</citedby><cites>FETCH-LOGICAL-c364t-b55640592a679e50b72ea8b3bb4ad19cb9b35816fc5bd3dac5f296413ccee3d93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.syapm.2006.01.008$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18329290$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>García-Estepa, Raul</creatorcontrib><creatorcontrib>Cánovas, David</creatorcontrib><creatorcontrib>Iglesias-Guerra, Fernando</creatorcontrib><creatorcontrib>Ventosa, Antonio</creatorcontrib><creatorcontrib>Csonka, Laszlo N.</creatorcontrib><creatorcontrib>Nieto, Joaquín J.</creatorcontrib><creatorcontrib>Vargas, Carmen</creatorcontrib><title>Osmoprotection of Salmonella enterica serovar Typhimurium by N γ -acetyldiaminobutyrate, the precursor of the compatible solute ectoine</title><title>Systematic and applied microbiology</title><description>N
γ
-acetyl-2,4-diaminobutyrate (NADA), the precursor of the compatible solute ectoine, was shown to function as an osmoprotectant for the non-halophilic bacterium
Salmonella enterica serovar Typhimurium. The addition of NADA-containing extracts of an ectoine synthase mutant of the broad salt-growing halophile
Chromohalobacter salexigens DSM 3043
T could alleviate the inhibitory effects of high salinity in
S. enterica, which lacks the ectoine biosynthetic pathway. NADA, purified from extracts of the mutant, protected
S. enterica against salinity stress. This osmoprotective effect was slightly lower than that of ectoine, but more potent than that of hydroxyectoine. Accumulation of purified NADA by
S. enterica was demonstrated by
13C-NMR spectroscopy and HPLC analysis. In addition, it was shown that NADA was taken up by
S. enterica via the ProP and ProU transport systems, which are known to transport glycine betaine and proline. This finding provides evidence that these permeases can recognize a diaminoacid that carries an unsubstituted
α
-amino group. This is the first time that NADA has been connected with osmoprotective functions in non-halophilic bacteria.</description><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Chromohalobacter salexigens</subject><subject>Compatible solutes</subject><subject>Ectoine</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydroxyectoine</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>N γ-acetyldiaminobutyrate</subject><subject>Osmoregulation</subject><subject>Salmonella enterica</subject><issn>0723-2020</issn><issn>1618-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNp9kEtu1TAUhi0EEpfCCph4Qkck-JH4xgMGVcVLquiAMraOnRPVV3EcbKdSdsB-2AdrIuFWYsboSEf_Q_9HyGvOas64eneq8wpzqAVjqma8Zqx7Qg5c8a5iumuekgM7ClkJJthz8iLnE2O80YofyM_bHOKcYkFXfJxoHOg3GEOccByB4lQweQc0Y4oPkOjdOt_7sCS_BGpX-pX-_kUrcFjWsfcQ_BTtUtYEBd_Sco90TuiWlGPag_eHi2GG4u2INMdxKUi34ugnfEmeDTBmfPV4L8j3jx_urj9XN7efvlxf3VROqqZUtm1Vw1otQB01tsweBUJnpbUN9Fw7q61sO64G19pe9uDaQWjVcOkcouy1vCCX59xt9I8FczHBZ7evnTAu2XAtdcd1twnlWehSzDnhYObkA6TVcGZ26uZk_lI3O3XDuNmob643j_GQHYxDgsn5_M_aSaGFZpvu_VmH29YHj8lk53Fy2PsNWTF99P_t-QMQtZ45</recordid><startdate>20061201</startdate><enddate>20061201</enddate><creator>García-Estepa, Raul</creator><creator>Cánovas, David</creator><creator>Iglesias-Guerra, Fernando</creator><creator>Ventosa, Antonio</creator><creator>Csonka, Laszlo N.</creator><creator>Nieto, Joaquín J.</creator><creator>Vargas, Carmen</creator><general>Elsevier GmbH</general><general>Elsevier</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>20061201</creationdate><title>Osmoprotection of Salmonella enterica serovar Typhimurium by N γ -acetyldiaminobutyrate, the precursor of the compatible solute ectoine</title><author>García-Estepa, Raul ; Cánovas, David ; Iglesias-Guerra, Fernando ; Ventosa, Antonio ; Csonka, Laszlo N. ; Nieto, Joaquín J. ; Vargas, Carmen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c364t-b55640592a679e50b72ea8b3bb4ad19cb9b35816fc5bd3dac5f296413ccee3d93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Chromohalobacter salexigens</topic><topic>Compatible solutes</topic><topic>Ectoine</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydroxyectoine</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>N γ-acetyldiaminobutyrate</topic><topic>Osmoregulation</topic><topic>Salmonella enterica</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>García-Estepa, Raul</creatorcontrib><creatorcontrib>Cánovas, David</creatorcontrib><creatorcontrib>Iglesias-Guerra, Fernando</creatorcontrib><creatorcontrib>Ventosa, Antonio</creatorcontrib><creatorcontrib>Csonka, Laszlo N.</creatorcontrib><creatorcontrib>Nieto, Joaquín J.</creatorcontrib><creatorcontrib>Vargas, Carmen</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Systematic and applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>García-Estepa, Raul</au><au>Cánovas, David</au><au>Iglesias-Guerra, Fernando</au><au>Ventosa, Antonio</au><au>Csonka, Laszlo N.</au><au>Nieto, Joaquín J.</au><au>Vargas, Carmen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Osmoprotection of Salmonella enterica serovar Typhimurium by N γ -acetyldiaminobutyrate, the precursor of the compatible solute ectoine</atitle><jtitle>Systematic and applied microbiology</jtitle><date>2006-12-01</date><risdate>2006</risdate><volume>29</volume><issue>8</issue><spage>626</spage><epage>633</epage><pages>626-633</pages><issn>0723-2020</issn><eissn>1618-0984</eissn><coden>SAMIDF</coden><abstract>N
γ
-acetyl-2,4-diaminobutyrate (NADA), the precursor of the compatible solute ectoine, was shown to function as an osmoprotectant for the non-halophilic bacterium
Salmonella enterica serovar Typhimurium. The addition of NADA-containing extracts of an ectoine synthase mutant of the broad salt-growing halophile
Chromohalobacter salexigens DSM 3043
T could alleviate the inhibitory effects of high salinity in
S. enterica, which lacks the ectoine biosynthetic pathway. NADA, purified from extracts of the mutant, protected
S. enterica against salinity stress. This osmoprotective effect was slightly lower than that of ectoine, but more potent than that of hydroxyectoine. Accumulation of purified NADA by
S. enterica was demonstrated by
13C-NMR spectroscopy and HPLC analysis. In addition, it was shown that NADA was taken up by
S. enterica via the ProP and ProU transport systems, which are known to transport glycine betaine and proline. This finding provides evidence that these permeases can recognize a diaminoacid that carries an unsubstituted
α
-amino group. This is the first time that NADA has been connected with osmoprotective functions in non-halophilic bacteria.</abstract><cop>Jena</cop><pub>Elsevier GmbH</pub><doi>10.1016/j.syapm.2006.01.008</doi><tpages>8</tpages></addata></record> |
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| ispartof | Systematic and applied microbiology, 2006-12, Vol.29 (8), p.626-633 |
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| source | ScienceDirect Journals (5 years ago - present) |
| subjects | Bacteriology Biological and medical sciences Chromohalobacter salexigens Compatible solutes Ectoine Fundamental and applied biological sciences. Psychology Hydroxyectoine Microbiology Miscellaneous N γ-acetyldiaminobutyrate Osmoregulation Salmonella enterica |
| title | Osmoprotection of Salmonella enterica serovar Typhimurium by N γ -acetyldiaminobutyrate, the precursor of the compatible solute ectoine |
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