Multiple determinants in the coding region of Pea seed- borne mosaic virus P3 are involved in virulence against sbm-2 resistance
Viral determinants for overcoming Pisum sativum recessive resistance, sbm-2, against the potyvirus Pea seed-borne mosaic virus (PSbMV) were identified in the region encoding the N-terminal part of the P3 protein. Codons conserved between sbm-2 virulent isolates in this region: Q21, K30 and H122 were...
Gespeichert in:
| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 2006-11, Vol.355 (1), p.52-61 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 61 |
|---|---|
| container_issue | 1 |
| container_start_page | 52 |
| container_title | Virology (New York, N.Y.) |
| container_volume | 355 |
| creator | Hjulsager, Charlotte Kristiane Olsen, Birgit Schlichter Jensen, Ditte Marie Kjær Cordea, Mirela Irina Krath, Britta N. Johansen, I. Elisabeth Lund, Ole Søgaard |
| description | Viral determinants for overcoming
Pisum sativum recessive resistance,
sbm-2, against the potyvirus
Pea seed-borne mosaic virus (PSbMV) were identified in the region encoding the N-terminal part of the P3 protein. Codons conserved between
sbm-2 virulent isolates in this region: Q21, K30 and H122 were found to specifically impair
sbm-2 virulence when mutated in selected genetic backgrounds. The corresponding amino acids, Gln21 and Lys30, are neighbored by P3 residues strongly conserved among potyviruses and His122 is conserved particularly in potyviral species infecting legumes. The strongest selective inhibition of
sbm-2 virulence, however, was observed by elimination of isolate specific length polymorphisms also located in the N-terminal part of the P3 protein. Length variation in N-terminal P3 is common between potyviral species. However, intra-species length polymorphism in this region was found only among PSbMV isolates. Our findings comply with a model for PSbMV pathotypes having evolved by a diversification of the P3 protein likely to extend to the level of function. |
| doi_str_mv | 10.1016/j.virol.2006.07.016 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_19395087</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0042682206004740</els_id><sourcerecordid>19395087</sourcerecordid><originalsourceid>FETCH-LOGICAL-c523t-78fd9f710284113f145417597241fcbb85f932a68f1e2b4eeaf41787d7191ed13</originalsourceid><addsrcrecordid>eNp9kE2LFDEQhoMo7rj6CwTNyVu3VemPdA4eZFk_YMUF3XNIdypjhu7OmHQPePOnm3YGvHkKqfepl-Jh7CVCiYDt20N58jGMpQBoS5Blnj1iOwTVFlDV-JjtAGpRtJ0QV-xZSgfIfynhKbvCVkEHdb1jv7-s4-KPI3FLC8XJz2ZeEvczX34QH4L1855H2vsw8-D4PRmeiGzB-xBn4lNIxg88H7Imfl9xEynvnsJ4IruVbMFI80Dc7I2f08JTPxUiNyafFpOD5-yJM2OiF5f3mj18uP1-86m4-_rx8837u2JoRLUUsnNWOYkguhqxclg3NcpGSVGjG_q-a5yqhGk7hyT6msi4nHfSSlRIFqtr9ubce4zh50pp0ZNPA42jmSmsSaOqVAOdzGB1BocYUork9DH6ycRfGkFv4vVB_xWvN_EapM6zvPXqUr_2E9l_OxfTGXh9BpwJ2uyjT_rhmwCsAGSHSnWZeHcmKGs4eYo6DX5zZ32kYdE2-P-e8AewN58y</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19395087</pqid></control><display><type>article</type><title>Multiple determinants in the coding region of Pea seed- borne mosaic virus P3 are involved in virulence against sbm-2 resistance</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Hjulsager, Charlotte Kristiane ; Olsen, Birgit Schlichter ; Jensen, Ditte Marie Kjær ; Cordea, Mirela Irina ; Krath, Britta N. ; Johansen, I. Elisabeth ; Lund, Ole Søgaard</creator><creatorcontrib>Hjulsager, Charlotte Kristiane ; Olsen, Birgit Schlichter ; Jensen, Ditte Marie Kjær ; Cordea, Mirela Irina ; Krath, Britta N. ; Johansen, I. Elisabeth ; Lund, Ole Søgaard</creatorcontrib><description>Viral determinants for overcoming
Pisum sativum recessive resistance,
sbm-2, against the potyvirus
Pea seed-borne mosaic virus (PSbMV) were identified in the region encoding the N-terminal part of the P3 protein. Codons conserved between
sbm-2 virulent isolates in this region: Q21, K30 and H122 were found to specifically impair
sbm-2 virulence when mutated in selected genetic backgrounds. The corresponding amino acids, Gln21 and Lys30, are neighbored by P3 residues strongly conserved among potyviruses and His122 is conserved particularly in potyviral species infecting legumes. The strongest selective inhibition of
sbm-2 virulence, however, was observed by elimination of isolate specific length polymorphisms also located in the N-terminal part of the P3 protein. Length variation in N-terminal P3 is common between potyviral species. However, intra-species length polymorphism in this region was found only among PSbMV isolates. Our findings comply with a model for PSbMV pathotypes having evolved by a diversification of the P3 protein likely to extend to the level of function.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1016/j.virol.2006.07.016</identifier><identifier>PMID: 16908044</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Amino Acid Substitution - genetics ; Codon - genetics ; codons ; Conserved Sequence ; conserved sequences ; disease resistance ; Immunity, Innate ; Molecular Sequence Data ; Mutation ; P3 protein ; Pathotype ; Pea seed-borne mosaic virus ; peas ; Pisum sativum ; Pisum sativum - virology ; Plant Diseases - virology ; plant viruses ; Polymorphism, Genetic ; Potyviridae ; Potyvirus ; Potyvirus - genetics ; Potyvirus - pathogenicity ; Recessive resistance ; Sequence alignment ; Sequence Deletion ; Sequence Homology, Amino Acid ; viral proteins ; Viral Proteins - chemistry ; Viral Proteins - genetics ; Viral Proteins - physiology ; virulence ; Virulence - genetics</subject><ispartof>Virology (New York, N.Y.), 2006-11, Vol.355 (1), p.52-61</ispartof><rights>2006 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c523t-78fd9f710284113f145417597241fcbb85f932a68f1e2b4eeaf41787d7191ed13</citedby><cites>FETCH-LOGICAL-c523t-78fd9f710284113f145417597241fcbb85f932a68f1e2b4eeaf41787d7191ed13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0042682206004740$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16908044$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hjulsager, Charlotte Kristiane</creatorcontrib><creatorcontrib>Olsen, Birgit Schlichter</creatorcontrib><creatorcontrib>Jensen, Ditte Marie Kjær</creatorcontrib><creatorcontrib>Cordea, Mirela Irina</creatorcontrib><creatorcontrib>Krath, Britta N.</creatorcontrib><creatorcontrib>Johansen, I. Elisabeth</creatorcontrib><creatorcontrib>Lund, Ole Søgaard</creatorcontrib><title>Multiple determinants in the coding region of Pea seed- borne mosaic virus P3 are involved in virulence against sbm-2 resistance</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>Viral determinants for overcoming
Pisum sativum recessive resistance,
sbm-2, against the potyvirus
Pea seed-borne mosaic virus (PSbMV) were identified in the region encoding the N-terminal part of the P3 protein. Codons conserved between
sbm-2 virulent isolates in this region: Q21, K30 and H122 were found to specifically impair
sbm-2 virulence when mutated in selected genetic backgrounds. The corresponding amino acids, Gln21 and Lys30, are neighbored by P3 residues strongly conserved among potyviruses and His122 is conserved particularly in potyviral species infecting legumes. The strongest selective inhibition of
sbm-2 virulence, however, was observed by elimination of isolate specific length polymorphisms also located in the N-terminal part of the P3 protein. Length variation in N-terminal P3 is common between potyviral species. However, intra-species length polymorphism in this region was found only among PSbMV isolates. Our findings comply with a model for PSbMV pathotypes having evolved by a diversification of the P3 protein likely to extend to the level of function.</description><subject>Amino Acid Sequence</subject><subject>Amino Acid Substitution - genetics</subject><subject>Codon - genetics</subject><subject>codons</subject><subject>Conserved Sequence</subject><subject>conserved sequences</subject><subject>disease resistance</subject><subject>Immunity, Innate</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>P3 protein</subject><subject>Pathotype</subject><subject>Pea seed-borne mosaic virus</subject><subject>peas</subject><subject>Pisum sativum</subject><subject>Pisum sativum - virology</subject><subject>Plant Diseases - virology</subject><subject>plant viruses</subject><subject>Polymorphism, Genetic</subject><subject>Potyviridae</subject><subject>Potyvirus</subject><subject>Potyvirus - genetics</subject><subject>Potyvirus - pathogenicity</subject><subject>Recessive resistance</subject><subject>Sequence alignment</subject><subject>Sequence Deletion</subject><subject>Sequence Homology, Amino Acid</subject><subject>viral proteins</subject><subject>Viral Proteins - chemistry</subject><subject>Viral Proteins - genetics</subject><subject>Viral Proteins - physiology</subject><subject>virulence</subject><subject>Virulence - genetics</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE2LFDEQhoMo7rj6CwTNyVu3VemPdA4eZFk_YMUF3XNIdypjhu7OmHQPePOnm3YGvHkKqfepl-Jh7CVCiYDt20N58jGMpQBoS5Blnj1iOwTVFlDV-JjtAGpRtJ0QV-xZSgfIfynhKbvCVkEHdb1jv7-s4-KPI3FLC8XJz2ZeEvczX34QH4L1855H2vsw8-D4PRmeiGzB-xBn4lNIxg88H7Imfl9xEynvnsJ4IruVbMFI80Dc7I2f08JTPxUiNyafFpOD5-yJM2OiF5f3mj18uP1-86m4-_rx8837u2JoRLUUsnNWOYkguhqxclg3NcpGSVGjG_q-a5yqhGk7hyT6msi4nHfSSlRIFqtr9ubce4zh50pp0ZNPA42jmSmsSaOqVAOdzGB1BocYUork9DH6ycRfGkFv4vVB_xWvN_EapM6zvPXqUr_2E9l_OxfTGXh9BpwJ2uyjT_rhmwCsAGSHSnWZeHcmKGs4eYo6DX5zZ32kYdE2-P-e8AewN58y</recordid><startdate>20061110</startdate><enddate>20061110</enddate><creator>Hjulsager, Charlotte Kristiane</creator><creator>Olsen, Birgit Schlichter</creator><creator>Jensen, Ditte Marie Kjær</creator><creator>Cordea, Mirela Irina</creator><creator>Krath, Britta N.</creator><creator>Johansen, I. Elisabeth</creator><creator>Lund, Ole Søgaard</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20061110</creationdate><title>Multiple determinants in the coding region of Pea seed- borne mosaic virus P3 are involved in virulence against sbm-2 resistance</title><author>Hjulsager, Charlotte Kristiane ; Olsen, Birgit Schlichter ; Jensen, Ditte Marie Kjær ; Cordea, Mirela Irina ; Krath, Britta N. ; Johansen, I. Elisabeth ; Lund, Ole Søgaard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c523t-78fd9f710284113f145417597241fcbb85f932a68f1e2b4eeaf41787d7191ed13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Amino Acid Sequence</topic><topic>Amino Acid Substitution - genetics</topic><topic>Codon - genetics</topic><topic>codons</topic><topic>Conserved Sequence</topic><topic>conserved sequences</topic><topic>disease resistance</topic><topic>Immunity, Innate</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>P3 protein</topic><topic>Pathotype</topic><topic>Pea seed-borne mosaic virus</topic><topic>peas</topic><topic>Pisum sativum</topic><topic>Pisum sativum - virology</topic><topic>Plant Diseases - virology</topic><topic>plant viruses</topic><topic>Polymorphism, Genetic</topic><topic>Potyviridae</topic><topic>Potyvirus</topic><topic>Potyvirus - genetics</topic><topic>Potyvirus - pathogenicity</topic><topic>Recessive resistance</topic><topic>Sequence alignment</topic><topic>Sequence Deletion</topic><topic>Sequence Homology, Amino Acid</topic><topic>viral proteins</topic><topic>Viral Proteins - chemistry</topic><topic>Viral Proteins - genetics</topic><topic>Viral Proteins - physiology</topic><topic>virulence</topic><topic>Virulence - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hjulsager, Charlotte Kristiane</creatorcontrib><creatorcontrib>Olsen, Birgit Schlichter</creatorcontrib><creatorcontrib>Jensen, Ditte Marie Kjær</creatorcontrib><creatorcontrib>Cordea, Mirela Irina</creatorcontrib><creatorcontrib>Krath, Britta N.</creatorcontrib><creatorcontrib>Johansen, I. Elisabeth</creatorcontrib><creatorcontrib>Lund, Ole Søgaard</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hjulsager, Charlotte Kristiane</au><au>Olsen, Birgit Schlichter</au><au>Jensen, Ditte Marie Kjær</au><au>Cordea, Mirela Irina</au><au>Krath, Britta N.</au><au>Johansen, I. Elisabeth</au><au>Lund, Ole Søgaard</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multiple determinants in the coding region of Pea seed- borne mosaic virus P3 are involved in virulence against sbm-2 resistance</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>2006-11-10</date><risdate>2006</risdate><volume>355</volume><issue>1</issue><spage>52</spage><epage>61</epage><pages>52-61</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><abstract>Viral determinants for overcoming
Pisum sativum recessive resistance,
sbm-2, against the potyvirus
Pea seed-borne mosaic virus (PSbMV) were identified in the region encoding the N-terminal part of the P3 protein. Codons conserved between
sbm-2 virulent isolates in this region: Q21, K30 and H122 were found to specifically impair
sbm-2 virulence when mutated in selected genetic backgrounds. The corresponding amino acids, Gln21 and Lys30, are neighbored by P3 residues strongly conserved among potyviruses and His122 is conserved particularly in potyviral species infecting legumes. The strongest selective inhibition of
sbm-2 virulence, however, was observed by elimination of isolate specific length polymorphisms also located in the N-terminal part of the P3 protein. Length variation in N-terminal P3 is common between potyviral species. However, intra-species length polymorphism in this region was found only among PSbMV isolates. Our findings comply with a model for PSbMV pathotypes having evolved by a diversification of the P3 protein likely to extend to the level of function.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>16908044</pmid><doi>10.1016/j.virol.2006.07.016</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0042-6822 |
| ispartof | Virology (New York, N.Y.), 2006-11, Vol.355 (1), p.52-61 |
| issn | 0042-6822 1096-0341 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_19395087 |
| source | MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Amino Acid Sequence Amino Acid Substitution - genetics Codon - genetics codons Conserved Sequence conserved sequences disease resistance Immunity, Innate Molecular Sequence Data Mutation P3 protein Pathotype Pea seed-borne mosaic virus peas Pisum sativum Pisum sativum - virology Plant Diseases - virology plant viruses Polymorphism, Genetic Potyviridae Potyvirus Potyvirus - genetics Potyvirus - pathogenicity Recessive resistance Sequence alignment Sequence Deletion Sequence Homology, Amino Acid viral proteins Viral Proteins - chemistry Viral Proteins - genetics Viral Proteins - physiology virulence Virulence - genetics |
| title | Multiple determinants in the coding region of Pea seed- borne mosaic virus P3 are involved in virulence against sbm-2 resistance |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T19%3A33%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Multiple%20determinants%20in%20the%20coding%20region%20of%20Pea%20seed-%20borne%20mosaic%20virus%20P3%20are%20involved%20in%20virulence%20against%20sbm-2%20resistance&rft.jtitle=Virology%20(New%20York,%20N.Y.)&rft.au=Hjulsager,%20Charlotte%20Kristiane&rft.date=2006-11-10&rft.volume=355&rft.issue=1&rft.spage=52&rft.epage=61&rft.pages=52-61&rft.issn=0042-6822&rft.eissn=1096-0341&rft_id=info:doi/10.1016/j.virol.2006.07.016&rft_dat=%3Cproquest_cross%3E19395087%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19395087&rft_id=info:pmid/16908044&rft_els_id=S0042682206004740&rfr_iscdi=true |