Effects of Immobilized rhBMP-2/atelocollagen in vivo and in vitro
The use of recombinant human bone morphogenetic protein - 2 (rhBMP-2) to induce ectopic bone formation requires a carrier. Atelocollagen, a biomaterial with a porous structure, excellent operational features and biocompatibility, is known to be an effective carrier for rhBMP-2. However, conventional...
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| Veröffentlicht in: | Journal of Hard Tissue Biology 2006, Vol.15(1), pp.27-33 |
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| container_title | Journal of Hard Tissue Biology |
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| creator | Tsujigiwa, Hidetsugu Rodriguez, Andrea P. Takagi, Tohru Long, Hu Hai Rui, Kan Lu, Zhenfu Li, Xie Wei Wen-Xin, Gao Tan, Jin Xiao, Jing |
| description | The use of recombinant human bone morphogenetic protein - 2 (rhBMP-2) to induce ectopic bone formation requires a carrier. Atelocollagen, a biomaterial with a porous structure, excellent operational features and biocompatibility, is known to be an effective carrier for rhBMP-2. However, conventionally used lyophilized rhBMP-2/atelocollagen mixture does not necessarily give adequate bone induction effect. In the present study, we evaluated the effect of immobilizing rhBMP-2 to succinylated type I atelocollagen on the cellular activity of ST2 cells and immobilized rhBMP-2/ atelocollagen and non immobilized rhBMP-2/atelocollagen implanted in subcutaneous pockets of Wister rats. Our results revealed that 1) Alkaline phosphatase activity confirmed the effectiveness of rhBMP-2/ succinylated type I atelocollagen immobilization in augmenting cellular activity. 2) Intracellular signaling continued for prolonged period when rhBMP-2 was immobilized to succinylated type I atelocollagen. 3) In rhBMP-2/atelocollagen implants were completely fully with new bone formation and cell proliferation. Whereas, in non immobilized rhBMP-2/atelocollagen implants showed new hard tissue in the periphery of the carrier with only collagen membrane in its center. This study indicated that immobilizing rhBMP-2 is an efficient method to increase bone induction. |
| doi_str_mv | 10.2485/jhtb.15.27 |
| format | Article |
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Atelocollagen, a biomaterial with a porous structure, excellent operational features and biocompatibility, is known to be an effective carrier for rhBMP-2. However, conventionally used lyophilized rhBMP-2/atelocollagen mixture does not necessarily give adequate bone induction effect. In the present study, we evaluated the effect of immobilizing rhBMP-2 to succinylated type I atelocollagen on the cellular activity of ST2 cells and immobilized rhBMP-2/ atelocollagen and non immobilized rhBMP-2/atelocollagen implanted in subcutaneous pockets of Wister rats. Our results revealed that 1) Alkaline phosphatase activity confirmed the effectiveness of rhBMP-2/ succinylated type I atelocollagen immobilization in augmenting cellular activity. 2) Intracellular signaling continued for prolonged period when rhBMP-2 was immobilized to succinylated type I atelocollagen. 3) In rhBMP-2/atelocollagen implants were completely fully with new bone formation and cell proliferation. Whereas, in non immobilized rhBMP-2/atelocollagen implants showed new hard tissue in the periphery of the carrier with only collagen membrane in its center. This study indicated that immobilizing rhBMP-2 is an efficient method to increase bone induction.</description><identifier>ISSN: 1341-7649</identifier><identifier>EISSN: 1880-828X</identifier><identifier>DOI: 10.2485/jhtb.15.27</identifier><language>eng</language><publisher>THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY</publisher><subject>Alkaline phosphatase ; Collagen ; Immobilization ; rhBMP-2 ; Smad family</subject><ispartof>Journal of Hard Tissue Biology, 2006, Vol.15(1), pp.27-33</ispartof><rights>2006 by The Hard Tissue Biology Network Association(JHTBNet)</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3937-b86fcae5199a2c1fdab3867f41d87932e576beae57a461d1424df9e026fbbed53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,27901,27902</link.rule.ids></links><search><creatorcontrib>Tsujigiwa, Hidetsugu</creatorcontrib><creatorcontrib>Rodriguez, Andrea P.</creatorcontrib><creatorcontrib>Takagi, Tohru</creatorcontrib><creatorcontrib>Long, Hu Hai</creatorcontrib><creatorcontrib>Rui, Kan</creatorcontrib><creatorcontrib>Lu, Zhenfu</creatorcontrib><creatorcontrib>Li, Xie Wei</creatorcontrib><creatorcontrib>Wen-Xin, Gao</creatorcontrib><creatorcontrib>Tan, Jin</creatorcontrib><creatorcontrib>Xiao, Jing</creatorcontrib><creatorcontrib>China Medical University</creatorcontrib><creatorcontrib>Dentistry and Pharmaceutical Sciences</creatorcontrib><creatorcontrib>Faculty of Dentistry</creatorcontrib><creatorcontrib>Ji Lin University</creatorcontrib><creatorcontrib>Graduate School of Medicine</creatorcontrib><creatorcontrib>Harbin Medical University</creatorcontrib><creatorcontrib>Dalian Medical University</creatorcontrib><creatorcontrib>Department of Virology</creatorcontrib><creatorcontrib>Okayama University</creatorcontrib><title>Effects of Immobilized rhBMP-2/atelocollagen in vivo and in vitro</title><title>Journal of Hard Tissue Biology</title><addtitle>J. Hard Tissue Biology.</addtitle><description>The use of recombinant human bone morphogenetic protein - 2 (rhBMP-2) to induce ectopic bone formation requires a carrier. Atelocollagen, a biomaterial with a porous structure, excellent operational features and biocompatibility, is known to be an effective carrier for rhBMP-2. However, conventionally used lyophilized rhBMP-2/atelocollagen mixture does not necessarily give adequate bone induction effect. In the present study, we evaluated the effect of immobilizing rhBMP-2 to succinylated type I atelocollagen on the cellular activity of ST2 cells and immobilized rhBMP-2/ atelocollagen and non immobilized rhBMP-2/atelocollagen implanted in subcutaneous pockets of Wister rats. Our results revealed that 1) Alkaline phosphatase activity confirmed the effectiveness of rhBMP-2/ succinylated type I atelocollagen immobilization in augmenting cellular activity. 2) Intracellular signaling continued for prolonged period when rhBMP-2 was immobilized to succinylated type I atelocollagen. 3) In rhBMP-2/atelocollagen implants were completely fully with new bone formation and cell proliferation. Whereas, in non immobilized rhBMP-2/atelocollagen implants showed new hard tissue in the periphery of the carrier with only collagen membrane in its center. This study indicated that immobilizing rhBMP-2 is an efficient method to increase bone induction.</description><subject>Alkaline phosphatase</subject><subject>Collagen</subject><subject>Immobilization</subject><subject>rhBMP-2</subject><subject>Smad family</subject><issn>1341-7649</issn><issn>1880-828X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNo9kE9LxDAQxYsouK5e_AQ9eRC65l-b5iTrsuqCogcFbyFNJm6WtnGTrqCf3ixVLzMP8pt5k5dl5xjNCKvLq816aGa4nBF-kE1wXaOiJvXbYdKU4YJXTBxnJzFuEKowF2KSzZfWgh5i7m2-6jrfuNZ9g8nD-ubxuSBXaoDWa9-26h363PX5p_v0uerNqIfgT7Mjq9oIZ799mr3eLl8W98XD091qMX8oNBWUF01dWa2gxEIoorE1qqF1xS3DpuaCEih51UACuGIVNpgRZqwARCrbNGBKOs0uxr0fwW93EAfZuaghXdaD30WJBRVMlCSBlyOog48xgJUfwXUqfEmM5D4luU9J4lISnuDbEe7AOK1a37euB7nxu9Cn30izZWszuEaSFJlECJcIy1ESngqloiKI7l2vx0WbOKSs_j1VGJxu4d8TjyUN_73otQoSevoDcI2H2g</recordid><startdate>20060101</startdate><enddate>20060101</enddate><creator>Tsujigiwa, Hidetsugu</creator><creator>Rodriguez, Andrea P.</creator><creator>Takagi, Tohru</creator><creator>Long, Hu Hai</creator><creator>Rui, Kan</creator><creator>Lu, Zhenfu</creator><creator>Li, Xie Wei</creator><creator>Wen-Xin, Gao</creator><creator>Tan, Jin</creator><creator>Xiao, Jing</creator><general>THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY</general><general>The Society for Hard Tissue Regenerative Biology</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope></search><sort><creationdate>20060101</creationdate><title>Effects of Immobilized rhBMP-2/atelocollagen in vivo and in vitro</title><author>Tsujigiwa, Hidetsugu ; Rodriguez, Andrea P. ; Takagi, Tohru ; Long, Hu Hai ; Rui, Kan ; Lu, Zhenfu ; Li, Xie Wei ; Wen-Xin, Gao ; Tan, Jin ; Xiao, Jing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3937-b86fcae5199a2c1fdab3867f41d87932e576beae57a461d1424df9e026fbbed53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Alkaline phosphatase</topic><topic>Collagen</topic><topic>Immobilization</topic><topic>rhBMP-2</topic><topic>Smad family</topic><toplevel>online_resources</toplevel><creatorcontrib>Tsujigiwa, Hidetsugu</creatorcontrib><creatorcontrib>Rodriguez, Andrea P.</creatorcontrib><creatorcontrib>Takagi, Tohru</creatorcontrib><creatorcontrib>Long, Hu Hai</creatorcontrib><creatorcontrib>Rui, Kan</creatorcontrib><creatorcontrib>Lu, Zhenfu</creatorcontrib><creatorcontrib>Li, Xie Wei</creatorcontrib><creatorcontrib>Wen-Xin, Gao</creatorcontrib><creatorcontrib>Tan, Jin</creatorcontrib><creatorcontrib>Xiao, Jing</creatorcontrib><creatorcontrib>China Medical University</creatorcontrib><creatorcontrib>Dentistry and Pharmaceutical Sciences</creatorcontrib><creatorcontrib>Faculty of Dentistry</creatorcontrib><creatorcontrib>Ji Lin University</creatorcontrib><creatorcontrib>Graduate School of Medicine</creatorcontrib><creatorcontrib>Harbin Medical University</creatorcontrib><creatorcontrib>Dalian Medical University</creatorcontrib><creatorcontrib>Department of Virology</creatorcontrib><creatorcontrib>Okayama University</creatorcontrib><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><jtitle>Journal of Hard Tissue Biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tsujigiwa, Hidetsugu</au><au>Rodriguez, Andrea P.</au><au>Takagi, Tohru</au><au>Long, Hu Hai</au><au>Rui, Kan</au><au>Lu, Zhenfu</au><au>Li, Xie Wei</au><au>Wen-Xin, Gao</au><au>Tan, Jin</au><au>Xiao, Jing</au><aucorp>China Medical University</aucorp><aucorp>Dentistry and Pharmaceutical Sciences</aucorp><aucorp>Faculty of Dentistry</aucorp><aucorp>Ji Lin University</aucorp><aucorp>Graduate School of Medicine</aucorp><aucorp>Harbin Medical University</aucorp><aucorp>Dalian Medical University</aucorp><aucorp>Department of Virology</aucorp><aucorp>Okayama University</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Immobilized rhBMP-2/atelocollagen in vivo and in vitro</atitle><jtitle>Journal of Hard Tissue Biology</jtitle><addtitle>J. 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Our results revealed that 1) Alkaline phosphatase activity confirmed the effectiveness of rhBMP-2/ succinylated type I atelocollagen immobilization in augmenting cellular activity. 2) Intracellular signaling continued for prolonged period when rhBMP-2 was immobilized to succinylated type I atelocollagen. 3) In rhBMP-2/atelocollagen implants were completely fully with new bone formation and cell proliferation. Whereas, in non immobilized rhBMP-2/atelocollagen implants showed new hard tissue in the periphery of the carrier with only collagen membrane in its center. This study indicated that immobilizing rhBMP-2 is an efficient method to increase bone induction.</abstract><pub>THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY</pub><doi>10.2485/jhtb.15.27</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Alkaline phosphatase Collagen Immobilization rhBMP-2 Smad family |
| title | Effects of Immobilized rhBMP-2/atelocollagen in vivo and in vitro |
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