Biophysical Characterization of the Sterol Demethylase P450 from Mycobacterium tuberculosis, Its Cognate Ferredoxin, and Their Interactions

Mycobacterium tuberculosis encodes a P450 of the sterol demethylase family (CYP51) chromosomally located adjacent to a ferredoxin (Fdx). CYP51 and Fdx were purified to homogeneity and characterized. Spectroscopic analyses were consistent with cysteinate- and aqua-ligated heme iron in CYP51. An ε419...

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Veröffentlicht in:	Biochemistry (Easton) 2006-07, Vol.45 (27), p.8427-8443
Hauptverfasser:	McLean, Kirsty J, Warman, Ashley J, Seward, Harriet E, Marshall, Ker R, Girvan, Hazel M, Cheesman, Myles R, Waterman, Michael R, Munro, Andrew W
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Bacterial Proteins - chemistry Bacterial Proteins - genetics Carbon Monoxide - chemistry Cytochrome P-450 Enzyme System - chemistry Cytochrome P-450 Enzyme System - genetics Ferredoxins - chemistry Heme - chemistry Hydrogen-Ion Concentration Iron - chemistry Kinetics Molecular Sequence Data Mycobacterium tuberculosis Mycobacterium tuberculosis - enzymology Oxidation-Reduction Oxidoreductases - chemistry Oxidoreductases - genetics Potentiometry Spectrum Analysis Sterol 14-Demethylase
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