Molecular Cloning and Characterization of Ribosomal Protein RPS9 in Echinococcus granulosus
Ribosomal protein S9 (RPS9) is an essential functional gene that participates in DNA repair and developmental regulations. A sequence homolog of RPS9 has been found to be upregulated in the protoscoleces (PSCs) of Echinococcus granulosus treated with artemisinin. However, E. granulosus RPS9 (EgRPS9)...
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| Veröffentlicht in: | The Journal of parasitology 2017-12, Vol.103 (6), p.699-707 |
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| container_title | The Journal of parasitology |
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| creator | Wen, L. M Lü, G. D Zhao, J Lu, S Gao, H. J Chen, B Ma, Y. F Xiao, Y. F Yuan, Y Zhang, H. B Liu, H Wang, J. H |
| description | Ribosomal protein S9 (RPS9) is an essential functional gene that participates in DNA repair and developmental regulations. A sequence homolog of RPS9 has been found to be upregulated in the protoscoleces (PSCs) of Echinococcus granulosus treated with artemisinin. However, E. granulosus RPS9 (EgRPS9) has not been identified before. In the present study, the 657-base pair (bp) cDNA encoding EgRPS9 was cloned. Amino acid sequence analysis showed that EgRPS9 was similar to the RSP9 proteins from Schistosoma japonicum (SjRPS9, 86%) and Schistosoma mansoni (SmRPS9, 79%). Phylogenetic tree analysis showed that EgRPS9, SmRPS9, and SjRPS9 were clustered together. We detected the EgRPS9 gene and protein expression in PSCs exposed to artesunate (AS) which displayed a dose-dependent reduction in PSC viability for 24 hr. The results showed that the EgRPS9 ratio of the 10-μM AS-treated (P < 0.01) and 40-μM AS-treated (P < 0.05) groups were increased from that of the control group. In addition, the level of reactive oxygen species (ROS) in the AS-treated groups increased in a dose-dependent manner compared to the level in the control group. In conclusion, the expression of EgRPS9 could be induced by ROS and might participate in the oxidative damage-based anti-parasite mechanism of AS treatment. |
| doi_str_mv | 10.1645/16-164 |
| format | Article |
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M ; Lü, G. D ; Zhao, J ; Lu, S ; Gao, H. J ; Chen, B ; Ma, Y. F ; Xiao, Y. F ; Yuan, Y ; Zhang, H. B ; Liu, H ; Wang, J. H</creator><creatorcontrib>Wen, L. M ; Lü, G. D ; Zhao, J ; Lu, S ; Gao, H. J ; Chen, B ; Ma, Y. F ; Xiao, Y. F ; Yuan, Y ; Zhang, H. B ; Liu, H ; Wang, J. H</creatorcontrib><description>Ribosomal protein S9 (RPS9) is an essential functional gene that participates in DNA repair and developmental regulations. A sequence homolog of RPS9 has been found to be upregulated in the protoscoleces (PSCs) of Echinococcus granulosus treated with artemisinin. However, E. granulosus RPS9 (EgRPS9) has not been identified before. In the present study, the 657-base pair (bp) cDNA encoding EgRPS9 was cloned. Amino acid sequence analysis showed that EgRPS9 was similar to the RSP9 proteins from Schistosoma japonicum (SjRPS9, 86%) and Schistosoma mansoni (SmRPS9, 79%). Phylogenetic tree analysis showed that EgRPS9, SmRPS9, and SjRPS9 were clustered together. We detected the EgRPS9 gene and protein expression in PSCs exposed to artesunate (AS) which displayed a dose-dependent reduction in PSC viability for 24 hr. The results showed that the EgRPS9 ratio of the 10-μM AS-treated (P < 0.01) and 40-μM AS-treated (P < 0.05) groups were increased from that of the control group. In addition, the level of reactive oxygen species (ROS) in the AS-treated groups increased in a dose-dependent manner compared to the level in the control group. In conclusion, the expression of EgRPS9 could be induced by ROS and might participate in the oxidative damage-based anti-parasite mechanism of AS treatment.</description><identifier>ISSN: 0022-3395</identifier><identifier>EISSN: 1937-2345</identifier><identifier>DOI: 10.1645/16-164</identifier><identifier>PMID: 28902565</identifier><language>eng</language><publisher>United States: American Society of Parasitologists</publisher><subject>Amino acid sequence ; Amino acids ; Antimicrobial agents ; Artemisinin ; Artesunate ; Chemotherapy ; Cloning ; Cysts ; Deoxyribonucleic acid ; DNA ; DNA repair ; Drug dosages ; Echinococcus granulosus ; Gene expression ; GENETICS-EVOLUTION ; Genomics ; Homology ; Insects ; Nucleotide sequence ; Oxidative stress ; Parasites ; Parasitic diseases ; Parasitology ; Phylogeny ; Proteins ; Reactive oxygen species ; Regulation ; Ribosomal protein S9 ; Schistosoma japonicum ; Schistosoma mansoni ; Viability</subject><ispartof>The Journal of parasitology, 2017-12, Vol.103 (6), p.699-707</ispartof><rights>American Society of Parasitologists 2017</rights><rights>Copyright Allen Press Publishing Services Dec 2017</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b358t-17a4fe63156a9778ad736b0bb3ac98d88d1f598845da7ee081e8a6a9c91097563</citedby><cites>FETCH-LOGICAL-b358t-17a4fe63156a9778ad736b0bb3ac98d88d1f598845da7ee081e8a6a9c91097563</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/44810483$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/44810483$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28902565$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wen, L. M</creatorcontrib><creatorcontrib>Lü, G. D</creatorcontrib><creatorcontrib>Zhao, J</creatorcontrib><creatorcontrib>Lu, S</creatorcontrib><creatorcontrib>Gao, H. J</creatorcontrib><creatorcontrib>Chen, B</creatorcontrib><creatorcontrib>Ma, Y. F</creatorcontrib><creatorcontrib>Xiao, Y. F</creatorcontrib><creatorcontrib>Yuan, Y</creatorcontrib><creatorcontrib>Zhang, H. B</creatorcontrib><creatorcontrib>Liu, H</creatorcontrib><creatorcontrib>Wang, J. H</creatorcontrib><title>Molecular Cloning and Characterization of Ribosomal Protein RPS9 in Echinococcus granulosus</title><title>The Journal of parasitology</title><addtitle>J Parasitol</addtitle><description>Ribosomal protein S9 (RPS9) is an essential functional gene that participates in DNA repair and developmental regulations. A sequence homolog of RPS9 has been found to be upregulated in the protoscoleces (PSCs) of Echinococcus granulosus treated with artemisinin. However, E. granulosus RPS9 (EgRPS9) has not been identified before. In the present study, the 657-base pair (bp) cDNA encoding EgRPS9 was cloned. Amino acid sequence analysis showed that EgRPS9 was similar to the RSP9 proteins from Schistosoma japonicum (SjRPS9, 86%) and Schistosoma mansoni (SmRPS9, 79%). Phylogenetic tree analysis showed that EgRPS9, SmRPS9, and SjRPS9 were clustered together. We detected the EgRPS9 gene and protein expression in PSCs exposed to artesunate (AS) which displayed a dose-dependent reduction in PSC viability for 24 hr. The results showed that the EgRPS9 ratio of the 10-μM AS-treated (P < 0.01) and 40-μM AS-treated (P < 0.05) groups were increased from that of the control group. In addition, the level of reactive oxygen species (ROS) in the AS-treated groups increased in a dose-dependent manner compared to the level in the control group. In conclusion, the expression of EgRPS9 could be induced by ROS and might participate in the oxidative damage-based anti-parasite mechanism of AS treatment.</description><subject>Amino acid sequence</subject><subject>Amino acids</subject><subject>Antimicrobial agents</subject><subject>Artemisinin</subject><subject>Artesunate</subject><subject>Chemotherapy</subject><subject>Cloning</subject><subject>Cysts</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA repair</subject><subject>Drug dosages</subject><subject>Echinococcus granulosus</subject><subject>Gene expression</subject><subject>GENETICS-EVOLUTION</subject><subject>Genomics</subject><subject>Homology</subject><subject>Insects</subject><subject>Nucleotide sequence</subject><subject>Oxidative stress</subject><subject>Parasites</subject><subject>Parasitic diseases</subject><subject>Parasitology</subject><subject>Phylogeny</subject><subject>Proteins</subject><subject>Reactive oxygen species</subject><subject>Regulation</subject><subject>Ribosomal protein S9</subject><subject>Schistosoma japonicum</subject><subject>Schistosoma mansoni</subject><subject>Viability</subject><issn>0022-3395</issn><issn>1937-2345</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp10E1LHTEUBuBQKvVq6z-wBCriZtpkkkxOlnLxC5SKbVddDJlMRnOZm6PJzML-eiPXuhBcJO_ifTgJh5A9zr7zRqofvKlKfiALboSuaiHVR7JgrK4rIYzaJjs5rxhjqpxPZLsGw2rVqAX5e4Wjd_NoE12OGEO8pTb2dHlnk3WTT-GfnQJGigO9CR1mXNuRXiecfIj05vqXoSVP3F2I6NC5OdPbZOM8Yp7zZ7I12DH7Ly-5S_6cnvxenleXP88ulseXVScUTBXXVg6-EVw11mgNttei6VjXCesM9AA9H5QBkKq32nsG3IMt1BnOjFaN2CVHm7n3CR9mn6d2HbLz42ijxzm3ZSUAqtagCv32hq5wTrH8rihQUEvQsqjDjXIJc05-aO9TWNv02HLWPq-7XM9Z4NeXcXO39v0r-7_fAvY3YJUnTK-9lMCZBFH6g03fBcTo33vnCbz4jZk</recordid><startdate>201712</startdate><enddate>201712</enddate><creator>Wen, L. 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M</au><au>Lü, G. D</au><au>Zhao, J</au><au>Lu, S</au><au>Gao, H. J</au><au>Chen, B</au><au>Ma, Y. F</au><au>Xiao, Y. F</au><au>Yuan, Y</au><au>Zhang, H. B</au><au>Liu, H</au><au>Wang, J. H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Cloning and Characterization of Ribosomal Protein RPS9 in Echinococcus granulosus</atitle><jtitle>The Journal of parasitology</jtitle><addtitle>J Parasitol</addtitle><date>2017-12</date><risdate>2017</risdate><volume>103</volume><issue>6</issue><spage>699</spage><epage>707</epage><pages>699-707</pages><issn>0022-3395</issn><eissn>1937-2345</eissn><abstract>Ribosomal protein S9 (RPS9) is an essential functional gene that participates in DNA repair and developmental regulations. A sequence homolog of RPS9 has been found to be upregulated in the protoscoleces (PSCs) of Echinococcus granulosus treated with artemisinin. However, E. granulosus RPS9 (EgRPS9) has not been identified before. In the present study, the 657-base pair (bp) cDNA encoding EgRPS9 was cloned. Amino acid sequence analysis showed that EgRPS9 was similar to the RSP9 proteins from Schistosoma japonicum (SjRPS9, 86%) and Schistosoma mansoni (SmRPS9, 79%). Phylogenetic tree analysis showed that EgRPS9, SmRPS9, and SjRPS9 were clustered together. We detected the EgRPS9 gene and protein expression in PSCs exposed to artesunate (AS) which displayed a dose-dependent reduction in PSC viability for 24 hr. The results showed that the EgRPS9 ratio of the 10-μM AS-treated (P < 0.01) and 40-μM AS-treated (P < 0.05) groups were increased from that of the control group. In addition, the level of reactive oxygen species (ROS) in the AS-treated groups increased in a dose-dependent manner compared to the level in the control group. In conclusion, the expression of EgRPS9 could be induced by ROS and might participate in the oxidative damage-based anti-parasite mechanism of AS treatment.</abstract><cop>United States</cop><pub>American Society of Parasitologists</pub><pmid>28902565</pmid><doi>10.1645/16-164</doi><tpages>9</tpages></addata></record> |
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| subjects | Amino acid sequence Amino acids Antimicrobial agents Artemisinin Artesunate Chemotherapy Cloning Cysts Deoxyribonucleic acid DNA DNA repair Drug dosages Echinococcus granulosus Gene expression GENETICS-EVOLUTION Genomics Homology Insects Nucleotide sequence Oxidative stress Parasites Parasitic diseases Parasitology Phylogeny Proteins Reactive oxygen species Regulation Ribosomal protein S9 Schistosoma japonicum Schistosoma mansoni Viability |
| title | Molecular Cloning and Characterization of Ribosomal Protein RPS9 in Echinococcus granulosus |
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